그럼에도 불구하고 나는 내일을 꿈꾼다

신선주,Sin, Seon-Ju 한국도서관협회 2006 圖協會報 Vol.47 No.4

Genome-wide identification and characterization of drought responsive MicroRNAs in Solanum tuberosum L.

신선주,이재희,권혁빈 한국유전학회 2017 Genes & Genomics Vol.39 No.11

MicroRNAs (miRNAs) are small non-coding RNAs that function as negative post-transcriptional regulators during plant growth and development in response to biotic and abiotic stresses. The objective of this study was to use next-generation sequencing (miRNA-sequencing) technology to identify drought responsive miRNAs from potato (Solanum tuberosum L.). After inducing small RNA under experimental conditions of aeration without watering for 1, 3, and 6 h compared to those at 0 h control, NGS was used to identify drought responsive miRNAs. From these drought responsive miRNAs, 21 known miRNAs and 19 novel miRNAs were selected. Of them, 38 were subjected to expression level analysis under four abiotic stresses (drought, salt, cold and abscisic acid) treatment through northern blot while two novel miRNAs were subjected to quantitative reverse transcription PCR (qRT-PCR) to determine their expression levels. The effects of other abiotic stresses such as cold, salt, and ABA treatments on the expression levels of the 38 drought responsive miRNAs were also analyzed. Putative target gene regulatory network of novel miRNAs were determined through computational analysis.

서울시 자치구 대표도서관 성인대상 프로그램 활성화를 위한 개선방안 연구

신선주,강순애,Shin, Seon-Ju,Kang, Soon-Ae 한국비블리아학회 2016 한국비블리아학회지 Vol.27 No.1

도서관은 자료의 제공, 수집, 정리, 보존이라는 전통적인 역할 뿐 아니라 사회적 변화의 흐름과 이용자의 요구사항을 반영한 다양한 프로그램을 운영하고 그 역할을 확장시키는 추세이다. 본 연구는 서울시 자치구 대표도서관들의 최근 5년간 성인대상 프로그램 운영현황을 파악하고, 성인대상 프로그램 담당자와 참여자를 대상으로 만족도 설문조사를 실시하였다. 이를 통해 프로그램 운영에 대한 개선사항과 이용자 요구사항을 종합적으로 분석함으로써 실제 현장에서 필요한 성인대상 프로그램의 활성화 방안을 제안하는 데 그 목적이 있다. 개선방안으로 지역 내 타 기관과의 프로그램 차별화, 새로운 프로그램 기획과 운영에 대한 운영역량교육 강화, 개별 도서관들의 차별화된 프로그램 정책, 이용자 요구사항에 대한 보다 전문적인 분석, 선호하는 프로그램에 대한 확대와 다양한 운영방식의 변화 등이 있다. As the needs of library users change diversely along with the rapid developments and changes in society, libraries have gone beyond the traditional role of providing, gathering, organizing and preserving materials and information to provide various program services in culture, interests, and practicality that accommodate cultural and educational needs. Public libraries, in particular, are reflecting their role as the representative educational and cultural institutions of the community and following social changes to expand the role of libraries and manage various programs. This study aims to vitalize adult-targeted programs in representative Seoul libraries by understanding the current situation of the aforementioned programs through the past five years, and ran a survey on the satisfaction levels of those who are in charge of the programs and those who utilize the programs. The purpose of the study is to comprehensively analyze the improvements needed for running the programs and the demands of users in order to propose ways to activate library programs for adults.

플래그쉽 스토어의 기호학적 해석에 관한 연구

신선주(Shin, Sun-Ju),류호창(Lyu, Ho-Chang) 한국실내디자인학회 2008 한국실내디자인학회 학술대회논문집 Vol.10 No.1

박물관의 감성적 전시 커뮤니케이션 증진방안에 관한 연구 -루돌프 슈타이너의 감각이론을 중심으로-

신선주 ( Sun Ju Shin ),최익서 ( Ik Seo Shin ) 한국공간디자인학회 2013 한국공간디자인학회논문집 Vol.8 No.4

(연구배경 및 목적)현대의 박물관은 전시를 매개로 하여 관람객과 시공간적 소통의 장으로 역할을 하고 있다. 전시를 통한 소통이란 내용의 전달 뿐만 아니라 주제와 메시지에 대한 공감을 통해 관람객의 반응을 유도하는 것이다. 따라서 본 연구는 박물관 유형에 따른 감성요소와 표현, 반응의 분석을 통해 감성적 전시 커뮤니케이션 증진 방안을 모색하는 것을 목적으로 한다. (연구방법)이러한 목적 달성을 위해 본 연구는 루돌프 슈타이너의 12감각론과 커뮤니케이션 특성에 대한 이론 고찰을 통해 박물관의 감성적 전시 커뮤니케이션 모델을 도출하고 분석매트릭스를 구성하였다. 그리고 박물관의 세 가지 유형을 대표하는 박물관들을 선정하여 매트릭스를 적용시켜 평가하였다. (결과) 그 결과 박물관의 전시는 주제의 유형에 따라 전시공간에 나타나는 감성요소와 표현의 차이가 있으며 감성반응도 다름을 도출하여 세 가지 차별화 방안에 대한 방향성을 제시하였다. 본 연구가 감성적 전시 커뮤니케이션을 위한 연출에의 적용 및 후속 연구에 기초자료로 활용되길 기대한다. (Background and Purpose)Modern museums facilitate temporal and spatial communications with visitors through the medium of the exhibitions. Communication through exhibitions is designed to elicit responses from visitors by conveying contents and arousing sympathy with themes and messages. Therefore, the aim of this study is to identify methods for improving sensibility communication through exhibitions by analyzing elements, sensibility expressions and responses according to the types of museums.(Method)A model was extracted for this study based on the theoretical consideration of Rudolf Steiner`s twelve senses and communications characteristics; ultimately an analysis matrix was constructed. Then, the matrix was applied to exhibitions in three types of museums. (Result)Differences in sensibility elements, expressions and responses in exhibition spaces were based on themes. Consequently, directivity for three differentiated schemes was suggested. In conclusion, it is anticipated that this study will benefit museum directors who wish to communicate through sensibility exhibitions; additionally, the study provides basic data for further research.

뉴스 빅 데이터에 나타난 ‘독서’ 담론 분석 : 11개 중앙 일간지를 중심으로

신선주 ( Shin¸ Seonju ) 한국독서학회 2020 독서연구 Vol.0 No.57

본 연구는 뉴스 빅 데이터 분석시스템 ‘빅카인즈(BigKinds)’에 나타난 ‘독서’ 담론이 무엇인지 알아보기 위해 정부가 추진하고 있는 ‘독서문화정책’시행 원년인 2009년을 분기점으로 이전 10년(1999년~2008년)과 이후 10년(2009년~2018년)으로 나누어 독서담론이 어떻게 나타나고 있는지 분석하고자 하였다. 또한 빅카인즈를 활용한 4가지 분석기법(키워드 검색, 연도별키워드 트렌드, 관계도 분석, 연관어 분석)을 통해 시기별 독서담론에서 어떤 지배적 담론이 들어가 있는지를 알아보기 위해 각 단계별 분석과정에서 나타나는 특징들을 대상으로 ‘독서’와 관련된 사회적 현상과 지배적인 독서담론을 들여다보고자 하였다. 연구 결과 다음과 같은 분석결과를 나타냈다. 첫째, 1999년부터 2018년까지 11개 중앙 일간지에 나타난 ‘독서’ 키워드의 기사가 갖는 독서담론의 비중을 확인한 결과 1차시기(1999~2008)에 비해 2차시기(2009~2018)의 키워드 추출 건수는 증가하였으나, 전체 기사 게재율 대비‘독서’키워드 추출건수 비율은 1,2차시기 모두 0.4%로 동일하게 나타났다. 이는 일간지에서 다루는 ‘독서’ 담론이 아주 미비함을 뜻하며, 뉴스기사에서 다루는 비율도 정부가 적극적으로 추진하는 ‘독서문화진흥정책’에 비해 지난 20년간큰 변화가 없음을 보여주었다. 둘째, 11개 중앙 일간지에 나타난 ‘독서’ 키워드 트렌드 분석 결과 ‘독서’가 다른 사회적 이슈들에 밀려 다뤄지지 않는 키워드에 속했으며, 일부 트렌드 추이가 올라가는 부분의 독서분야 이슈들을 살펴보면 정부가 추진하는 정책과 뉴스기사에서 다뤄지는 토픽과 밀접한 관련이 있음을 알 수 있다. 이는 ‘독서’가 개인의 담론으로는 한계점을 가지고 있으며, 정부가 적극적으로 정책적 이슈를 이끄는 것이 사회적인 독서담론에도 영향을 미치는 것으로 나타났다. 셋째, ‘독서’ 관계도 분석과 연관어 분석은 1,2차 시기별로 큰 차이를 보이지 않았다. 다만 정부나 민간이 추진하는 독서관련 사업에 따라 관계도와 연관어 분석에서는 키워드 빈도에서 약간의 변화가 있을 뿐이었다. 마지막으로 본 연구에서 제한점은 주제별 토픽분석기법인 ‘LDA’를 활용해서 토픽구조분석을 실시해야 했으나 실행하지 못함으로써 키워드 분석 중심으로 ‘독서’담론을 들여다보는 데 그쳤다. 또한 뉴스기사의 담론분석은 사회적 이슈가 큰 분야에서 보다 유용하게 분석을 이끌어낼 수 있다는 점과 ‘독서’와 같이 사회적 이슈가 적은 분야에서는 이슈분석보다 정부의 독서정책과 방향에 대한분석에 초점을 맞추는 것이 독서현장의 실행력을 높이는 데 더 주요한 연구가 될 것으로 생각된다. In 2009, the “Reading Culture Policy” was implemented in X, promoted by the Government. This study addresses the ten years before (1999-2008) and after (2009-2018) and analyzes how the reading discourses in each period appear to investigate the “reading” discourses in “Bigkinds,” a news-based big data analysis system. Further, the study investigates, by stage, the social phenomena related to “reading” for the characteristics in the analysis process. It also identifies the dominant reading discourses by period, through four analysis techniques (Keyword search, keyword trend by year, relationship analysis, and related word analysis), utilizing “Bigkinds.” Through this analysis, the following results were obtained. The importance of the reading discourses was examined by identifying articles containing the keyword “reading” in 11 major daily newspapers from 1999 through 2018. More articles were identified in Period 2 (2009- 2018) than in Period 1 (1999-2008), but, as a percentage of the total number of articles, the occurrence of the keyword “reading” remained the same (0.4%). This means that “reading” discourses infrequently feature in daily newspapers. Despite the “Reading Culture Promotion Policy” being actively promoted by the Government, their frequency of occurrence in news articles has remained largely unchanged for the past20 years. Second, analysis of the trend of the keyword “reading” in these 11 major daily newspapers revealed that it was not dealt with behind other social issues. Examining the issues in the field of reading during the period in which there was an increase in the trend revealed that they had close relationships with policies promoted by the Government and with the topics dealt with in the news articles. This shows that “reading” has a limitation as an individual’s discourse and that the Government’s active leading of policy issues affects social reading discourses. Third, relationship analysis (and related word analysis) concerning “reading” revealed that there was no significant difference between Periods 1 and 2. However, there was a slight change in the frequency of the keyword in the relationship and related word analysis, according to whether the reading-related projects were promoted by the government or the private sector. One limitation of this study is that topic structure analysis was not conducted utilizing “LDA,” a topic analysis technique. Rather, this study solely examined “reading” discourses, focusing on keyword analysis. Furthermore, analysis of the discourses of news articles could draw a more useful analysis in the areas that have more social issues. Additionally, focusing on the analysis of the Government’s reading policies and directions in areas that have fewer social issues would be more important regarding increasing executive ability in the field of reading.

Novel drought-responsive regulatory coding and non-coding transcripts from Oryza Sativa L.

권혁빈,신선주,안홍렬,정인욱,이성민,김선 한국유전학회 2016 Genes & Genomics Vol.38 No.10

Drought-stress can cause major economic loss and is a serious issue to address in agriculture. Defining the molecular pathways in how a plant responds to droughtstress may prove valuable in developing new drought-resistant plants. In this study, we identified several novel drought-responsive regulatory coding and noncoding transcripts in rice, Oryza sativa L., using the next generation sequencing (NGS) technique and bioinformatics analyses. We produced comprehensive NGS RNA sequencing data for mRNA, small RNA, and long noncoding RNA at the experimental conditions of aeration without watering for 1 and 6 h in compared with the 0 h control. We performed bioinformatics analysis to identify novel drought-responsive transcription factors (TFs), novel drought-responsive micro RNA (miRNA), and novel drought-responsive long noncoding RNAs (lncRNAs). These transcripts were validated by quantitative real-time PCR (qRT-PCR). We identified 18 TFs, ten lncRNAs and one miRNA as being novel drought-responsive regulatory transcripts. Computational analysis using a gene regulatory network showed that these transcripts were related to regulation of response to drought-stress.

Molecular characterization of cold stress-related transcription factors, CaEREBP-C1, -C2, -C3, and CaWRKY1A from Capsicum annuum L.

유병국,이재희,신선주,황을원,권혁빈 한국식물학회 2013 Journal of Plant Biology Vol.56 No.2

EREBP (ethylene responsive element binding protein) and WRKY (wizz-like transcription factor) are known to play roles in plant tolerance to abiotic stresses, such as low temperatures. Previously, we used cDNA microarrays and Northern blot analysis to determine the mechanisms responsible for those underlying defenses. These analyses led to the identification of CaEREBP-C1, -C2, -C3 and CaWRKY1A genes that encode the ethylene responsive element binding protein and wizz-like transcription factors, respectively, from hot pepper (Capsicum annuum). In that study, we demonstrated that the CaEREBP-C1, -C2, -C3 and CaWRKY1A genes were strongly induced by cold stress. Here, we used Tiplasmid and Agrobecterium-mediated transformation to engineer CaEREBP-C1, -C2, -C3 and CaWRKY1A under control of the CaMV 35S promoter for constitutive expression in transgenic plants. The resultant transgenic plants exhibited significantly increased tolerance to low temperatures. In addition, none of the CaEREBP-C1, -C2, -C3 and CaWRKY1A transgenic plants showed visible phenotypic alteration when compared to wild type plants. Taken together, these results suggest that CaEREBP-C1, -C2, -C3 and CaWRKY1A play important biological roles in conferring plant cold stress tolerance.

비보건계열 대학생의 고혈압 전단계에 관련된 지식, 태도, 건강증진행위가 자가간호역량에 미치는 영향

이채원,이지원,유민주,강지영,신선주,이지연,조은희 한국산학기술학회 2022 한국산학기술학회논문지 Vol.23 No.8

This study investigated the effects of pre-hypertension related to knowledge, attitude, and health-promoting behaviors on self-care competency in non-health department college students. Data were collected between August 25 to August 30, 2021, from college students in the 1st to 4th grades who were enrolled in non-health departments at two universities. The data collected were analyzed by percentage, mean and standard deviation, descriptive statistics, t-test, analysis of variance, difference analysis, correlation, and regression analysis using the SPSS/WIN 20.0 program. The results of this study revealed that the factor affecting self-care competency was health-promoting behaviors (β=.59, p<.001), and was determined to be statistically significant in the regression model (F=39.99, p<.001), having an explanatory power of 38.9%. Pre-hypertension related to knowledge and attitude had a positive correlation (r=.41, p<.001). Moreover, health-promoting behaviors also had a positive correlation with pre-hypertension related to knowledge (r=.24, p=.001) and attitude (r=.18, p=.014). The self-care competency had a statistically significant positive correlation with pre-hypertension related to knowledge (r=.23, p=.002), attitude (r=.24, p=.001), and health-promoting behaviors (r=.62, p<.001). Our results indicate that studies of pre-hypertension related to knowledge, attitude, and health-promoting behaviors are required to improve self-care competency among college students. This will contribute to the development of customized health-promoting programs. 본 연구는 비보건계열 대학생의 고혈압 전단계에 관련된 지식, 태도, 건강증진행위가 자가간호역량에 미치는 영향을 확인하기 위해 실시되었다. 2개 대학교 비보건계열 학과에 재학 중인 1~4학년 대학생을 대상으로 2021년 8월 25일부터 8월 30일까지 자료를 수집하였다. 수집된 자료는 SPSS/WIN 20.0 프로그램을 이용하여 백분율, 평균과 표준편차, 기술통계, t-검정과 분산분석, 상관관계, 그리고 회귀분석으로 분석하였다. 본 연구결과 자가간호역량의 영향요인은 건강증진행위(ꞵ=.59, p<.001)였고 회귀모형은 통계적으로 유의하였으며(F=39.99, p<.001), 모형의 설명력은 38.9%를 나타냈다. 고혈압 전단계에 관련된 지식과 태도는 정적 상관관계(r=.41, p<.001)로 나타났다. 건강증진행위는 고혈압 전단계에 관련된 지식(r=.24, p=.001)과 태도(r=.18, p=.014)의 정적 상관관계로 나타났다. 자가간호역량은 고혈압 전단계에 관련된 지식(r=.23, p=.002), 태도(r=.24, p=.001) 및 건강증진행위(r=.62, p<.001)와 통계적으로 유의한 정적 상관관계로 나타났다. 본 연구결과 대학생의 자가간호역량을 향상시키기 위해서는 대학생의 고혈압 전단계에 관련된 지식, 태도, 건강증진 행위에 대한 후속 연구가 필요하며, 이는 맞춤형 건강증진프로그램 개발로 이어져야 한다.

Identification of miR172 family members and their putative targets responding to drought stress in Solanum tuberosum

Eul-Won Hwang,신선주,박수철,Mi-Jeong Jeong,권혁빈 한국유전학회 2011 Genes & Genomics Vol.33 No.2

MicroRNAs (miRNAs) function as negative posttranscriptional regulators during plant development and in response to biotic and abiotic stress. In this study, we identified drought stress related miRNAs from sequencing reads obtained from second-generation sequencing. This method is useful for determination of miRNA expression profiles when genomic sequences are not available and for identification of putative miRNA by aligning reads with sequences from miRBase. Here, we present identification of a family of drought responsive miRNAs from potato plants, stu-miR172c,stu-miR172d, and stu-miR172e, and show expression profiles obtained in response to drought stress as well as their target mRNAs.