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Tylosin Production by Mutant Resistant to Oleic Acid
최두복,문옥란,윤미란,지성남,신대윤,Choi, Du-Bok,Choi, On-You,Moon, Ok-Ran,Yoon, Mi-Ran,Ji, Sung-Nam,Shin, Dae-Yewn Korean Society of Environmental Health 2005 한국환경보건학회지 Vol.31 No.5
Streptomyces fradiae로부터 Tylosin을 효율적으로 생산하기 위해 지방산 내성균주을 분리 했다. 여러 지방산중에서 oleic acid 1.6 g/l 이상이 첨가될 때 세포 성장이 완전히 저해 되었다. 그러나 oleic arid 1.2 g/l에서 얻어진 TM-224-1 균주는 최대 균체농도와 tylosin 생산이 얻어졌다. 또한 oleic acid 소비속도는 parent strain 비해 3.8배 증가했다. Oleic acid 내성균주, TM-224-1 균주와 parent strain을 이용해서 jar fermentor에서 균체농도, tylosin 생산, 그리고 rapeseed oil 소비를 표준조건하에 5일동안 비교하였다. TM-224-1 균주을 이용할 경우 균체 농도는 초기에 parent strain비해 증가했으나 배양중반부터는 감소하기 시작했다. rapeseed oil 소비의 경우는 거의 비슷했다. 그러나 Tylosin 생산 수율은 parent strain 비해 약 3.2배 증가했다. When rapeseed oil as the carbon source was used for tylosin production from Streptomyces fradiae TP-1239 was very sensitive to oleic acid. Cell growth was restrained by adding 0.8 g/l of oleic acid to the culture broth. Mutant strain TM-224-1 resistant to 1.2 g/l of oleic acid was obtained by screening in solid and liquid media containing oleic acid. The uptake rate of oleic acid by TM-224-1 was approximately 3.8 fold higher than the parent strain. For comparing the TM-224-1 and the parent strain, batch cultures were carried out in a jar fermentor. Cell growth of TM-224-1 strain was higher than the parent strain after two days of culturing. However, after four days of culturing, it was similar to that of the parent strain. The amount of rapeseed oil consumed by TM-224-1 and the parent strain were 60.5 and 78.2 g/l, respectively. The production and yield of tylosin was aproximately 2.0 and 3.2 fold higher than the parent strain, respectively. From these results, it was concluded that this mutant, which was resistant to oleic acid, has improved tylosin production.
염화철 코팅 참나무 탄화물을 이용한 질산성질소 흡착 특성
김정애 ( Jeong Ae Kim ),정경훈 ( Kyung Hoon Cheong ),신대윤 ( Dae Yun Shin ),최형일 ( Hyung Il Choi ),윤지현 ( Ji Hyun Yoon ) 한국수처리학회 2012 한국수처리학회지 Vol.20 No.1
In recent years, the nitrate nitrogen in lakes, rivers and underground water has become a serious issue. Charcoal processing is one of the techniques of effectively using wood chip. In this study, oak charcoal was coated with FeCl3 solution. The adsorption behavior of nitrate nitrogen was investigated from aqueous solution using coated oak charcoal. The removal efficiency of nitrate was increased with increasing of dosage of adsorbents. The degree of removal of nitrate nitrogen was found to be dependent on temperature and it is increased as the temperature increases. Adsorption equilibrium data of nitrate nitrogen on oak charcoal reasonably fitted Langmuir isotherm models. The adsorption energy obtained from D-R model was 10.3 kJ/mole at 20℃. indicating an ion exchange process as primary adsorption mechanism. The adsorbents was regenerated using KCI solution, and recovery rate was 75-76% at 1 mol of KCI. Overall, it is expected that the oak charcoal obtained by this method can contribute to the purification of water polluted nitrate nitrogen.
Disiloxane spacer를 갖는 액정 중합체의 합성 및 특성 연구
전윤,신성의,송연호,권규혁,김선,신대윤,최형일,최재곤 조선대학교 생산기술연구소 1983 生産技術硏究 Vol.1983 No.-
Two closely related copolymers that contain a di (oxyphenyl) terephthalate or a hydroquinone ring as a rnesogenic unit interconnected by a decamethylene and a disiloxane as flexible spacers in the main chain were synthesized and characterized for their mesogenic properties. These two polymers showed nematic mesogenic behavior and the phase transitions from solid to nematic phases and from nematic to isotropic were reversible, that is, antiotropic. The effect of spacers on the mesogenic unit on the stability of the mesophase or on the orientational order of polymer units in the mesophase could be rationalized on the bases of observed thermodynamic data.