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지방자치단체 광고효용성에 대한 탐색적 연구: KTX 광고노출 환경을 중심으로
송승열,임상국,김정규 한국멀티미디어학회 2020 멀티미디어학회논문지 Vol.23 No.1
Along side with the rapid growth of local governments' advertising bills, there are few studies focused on the effectiveness of these ads. Especially one of the media being used by the local governments is the Korea Express Train (KTX), where they advertise in the train coaches’ KTX video monitor. Unfortunately the ads in KTX are exposed without audio mostly. The current study, therefore, probed on the effectiveness of these ads. This study utilized transportation theory and content analysis methodology to give insight to its discourse. We established two analysis units (camera and subtitles), and then analyzed 107 local government ads. From the camera analysis, it is observed that local governments’ festival and tour promotion ads more often employ dynamic angles such as drone shot and long shot. Also, from subtitles usage analysis, it is observed that many of the ads make use of large size titles and subtitles which could prevent viewers seeing visual shots. In the special case audio-less KTX ads, this study recommends emphasis on subtitles which will enhance the ad effectiveness of the ad messages.
소독제 과산화수소에 노출된 Legionella pneumophila의 생존력
송승열,오계헌 순천향대학교 기초과학연구소 2000 순천향자연과학연구 논문집 Vol.6 No.2
Survival of L. pneumophila exposed by hydrogen peroxide as a disinfectant was monitored. Initially morphological and physiological characteristics were examined. The strain was small Gram-negative cocco-bacillus and could not degrade carbohydrate. Survival of L. pneumophila was examined in the buffered charcoal yeast extract medium containing 0-5% hydrogen peroxide. The survival rates of cells decreased with increasing concentrations of hydrogen peroxide. The growth of L. pneumophila was significantly inhibited by the exposure of hydrogen peroxide. When the strain was treated with >0.01% hydrogen peroxide, the cells could not grow on the medium. Survival rate of cells decreased when trated with >0.01% of concentrations of hydrogen peroxide. Time-survival studies showed that exposure of L. pneumophila cells to 0.3% hydrogen peroxide resulted in at least 80% killing after 90 min and not detected the survivors at 120 min. No cells were grown at the concentrations of 1%> hydrogen peroxide. As the result, hydrogen peroxide suggested a good candidate for the control agent of L. pneumophila.
Benzoate에서 배양된 Acinetobacter baumannii로부터 Catechol 1,2-dixoygenase의 정제와 촉매적 성질
송승열,오계헌 순천향대학교 기초과학연구소 2001 순천향자연과학연구 논문집 Vol.7 No.2
The purpose of this work was to perform the catalytic properties and sequence analysis of catechol 1,2-dioxygenase (C1,2O) purified from Acinetobacter baumannii which was grown on benzoate as a carbon source. C1,20 demonstrated its enzyme activity to other substrates, catechol and 4-methylcatechol. The optimum temperature of C1,20 was 35℃, and the optimal pH was in the range from pH 7.5 to 9. Ag^+, Hg^+, and Cu^+2 showed inhibitory effect on the activity of C1,20. Molecular weight of the enzyme was determined to approximately 36 kDa by SDS-PAGE. N-terminal amino acid sequence of C1,20 was analysed as ^1MNYQQIDALVKQMNVDTAKG^20 and exhibited 95% sequence homology with that of C1,20 from Acinetobacter radioresistens. For internal sequencing analysis, trypsin digestion and peptide mapping were performed. Molecular weights of three digested peptide fragments were analyzed as 966.3 dalton, 1933.8 dalton and 2081.7 dalton by MALDI-TOF, which were matched with internal sequences (^1SQSDFNLRR^9, ^1TIEGPLYVAGAPESVGFAR^19, ^1HGNRPSHVHYFVSAPGYR^18)of Acinetobacter radioresistens.
옥외영상광고 효과에 대한 탐색적 담론 : 정부 행정기관의 옥외영상광고 노출 환경을 중심으로
송승열,곽주영,김정규 한국멀티미디어학회 2020 멀티미디어학회논문지 Vol.23 No.11
Advertising is a communication that aims to persuade you through communication. Local governments are enforcing a lot of budget for outdoor advertisements and exposing advertisements to various media. However, in the case of outdoor advertisements, advertisements are executed without considering the specificity of the exposure environment, so it is inferior to the budget input. Therefore, this study aims to help local advertisement agents, producers, and related parties by classifying components by combining video analysis methodology and video ad analysis methodology for effective message delivery and immersion in local outdoor advertising. As a discussion to provide an efficient production methodology for outdoor advertising video production in the future, it is an extension of exploratory research through content analysi
환경오염원으로서 Benzoate에 노출된 Acinetobacter sp. KS-1의 생존과 형태변화
송승열,오계헌 순천향대학교 기초과학연구소 2002 순천향자연과학연구 논문집 Vol.8 No.2
The purpose of this work was to investigate the survival and morphological changes of Acinetobacter sp. KS-1 when exposed by benzoate as an environmental stressor. The strain KS-1 was isolated from environmental samples around hospitals and could utilize benzoate as the sole source of carbon and energy. Strain KS-1 was examined the physiological and biological characteristics. Physiological analysis using BIOLOG GN2 MicroPlate system was performed to identify the strain, which could be assigned to genus Acinetobacter and designated as Acinetobacter sp. KS-1. Microscopic examination of the strain revealed Gram-negative and short rod cells. Survival of Acinetobacter sp. KS-1 cells was examined in different concentrations of benzoate. Survival of the KS-1 cell had less effect on the concentrations of 10 mM, 100 mM, or 500 mM of benzoate, whereas significant decrease of survival rates was measured at 1 M benzoate. Scanning electron microscopy was used to examine the morphological changes of Acinetobacter sp. KS-1 cells depending on the different concentrations of benzoate. However little morphological changes of the cells were observed in this experiments.
Benzoate 분해세균 Acinetobacter sp. kS-1에서 분리된 catechol 1,2-dioxygenase의 특성 및 N 말단 아미노산 서열 분석
오계헌,송승열,김승일,윤경하 한국미생물학회 2002 미생물학회지 Vol.38 No.2
단일 탄소원 및 에너지원으로 benzoate를 이용하는 Acinetobacter sp. KS-1에서 분리 정제한 catechol 1,2-dioxygenase (Cl,2O)의 특성과 아미노산 서 열을 분석하였다. Cl,2O는 catechol과 4-methylcatechol에 대해서 효소활성을 나타내었으며, 활성 최적온도는 $35^{\circ}C$이고, 활성 최적 pH는 7.5-9.0의 범위 내에 있었다. 효소활성 저해제로서 은, 수은, 그리고 구리는 Acinetobacter sp. KS-1의 Cl,2O 활성을 억제하였다. SDS-PAGE에 의해 측정된 Cl,2O의 분자량은 약 36 kDa 였으며, N-말단 아미노산 서 열을 분석한 결과, $^{1}MNYQQIDALVKQMNVDTAKG^{20}$로 Acinetobacter radioresistens의 Cl,2O와 95%의 유사성을 보여주었다. In-gel 아미노산 서열 분석을 위하여 trypsin 처리와 peptide mapping을 실시하였다. MALDI-TOF를 이용하여 trypsin으로 처리된 세 개의 peptide flagmen써 분자량을 분석한 결과 966.3 Da, 2081.7 Da, 그리고 1933.8 Da으로 각각 나타났는데, 이는 A. radioresistens의 Cl,2O와 내부 서 열$^{1}SQSDFNLRR^{9}\, ^{1}HGNRPSHVHYFNSAPGYR^{18}\, ^{1}TIEGPLYVAGAPESVGFAR^{19}$ 이 일치하는 것으로 분석되었다. N-말단 서열과 내부 서열을 바탕으로 primer를 제작하여 polymerase chain reaction을 실시하였다. The purpose of this work was to investigate the characterization and sequence of catechol 1,2-dioxygenase (Cl,2O) purified from Acinetobacter sp. KS-1 which was grown on benzoate as a sole carbon source. Cl,2O demonstrated its enzyme activity to catechol and 4-methylcatechol. The optimum temperature of Cl,2O was $35^{\circ}C$, and the optimal pH was in the range from pH 7.5 to 9.0. $Ag^{+}$, $Hg^{+}$, and $Cu^{2+}$ showed inhibitory effect on the activity of Cl,2O. Molecular weight of the enzyme was determined to approximately 36 kDa by SDS-PAGE and 7-terminal amino acid sequence of Cl,2O was analyzed as $^{1}MNYQQIDALVKQMNVDTAKG^{20}$and exhibited 95% sequence homology with that of Cl,2O from Acinetobacter radioresistens In addition, trypsin digestion and peptide mapping were performed for internal sequencing analysis. Molecular weights of three digested peptide fragments were analyzed as 966.3 Da, 1933.8 Da and 2081.7 Da by MALDI-TOF, which were matched with each internal sequences $^{1}SQSDFNLRR^{9}\, ^{1}HGNRPSHVHYFNSAPGYR^{18}\, ^{1}TIEGPLYVAGAPESVGFAR^{19}$) of. A. radioresistens. PCR product was amplified with the degenerated primers derived from N-terminal and each internal amino acid sequences.