현대인물탐구:우사 김규식의 민족통일노선과 좌우합작운동

송남현 북한연구소 1988 北韓 Vol.- No.196

복숭아 有名品種의 果實生長에 따른 生理解剖學的 硏究

宋南顯 圓光大學校大學院 1986 學位論叢 Vol.16 No.-

A series of experiments were carried out to understand more thoroughly the developmental pattern of "Yumyeong" peach fruit by measuring the growth rate and the relative growth rate (RGR) from diameter and weight measurements during the period from late of May to late of August, and also obtain the anatomical and physiological characteristics of pre-harvest drop of peach fruits throughout the effect of chemical treatments such as ethephon(400ppm), 2, 4, 5-TP(25 ppm, 50 ppm), and 2, 4, 5-TP(25 ppm) +GA(20 ppm) at one month before harvesting in 1985. The results obtained were summarized as follows: Experiment I. Growth and development pattern and pre-harvest drop rate In natural situation. 1. Peach fruits showed a single sigmoid curve in terms of the growth of diameter and fresh weight of the fruit. Fruit development could be classified into three stage as the stage I, increasing rapidly diameter and weight of fruit from late May to late June, and the stage II, increasing slowly to middle to late July, and then the stage III, increasing rapidly again at August. 2. During early stage, fruit growth on the suture and length diameter were greater than that on the cheek diameter, whereas the growth of cheek diameter was greater after August. 3. The increase to a peak in the growth velocity of fruit diameter was about two weeks earlier than that in the growth velocity of fruit fresh weight. The growth peak of fresh and dry weight also was one to two weeks earlier than that of fruit diameter. 4. Dry matter percent of whole fruit decreased rapidly as the fruit diameter increased, tended to be increased on the overall growth rate slowed down markedly. 5. The maximum of fruit stalk diameter and length were reached at late June and late July, respectively. And the fruit stalk length was coincided with the sunken-depth of fruit at late July. 6. The growth pattern of fruit sunken-depth was very similar to that of fruit fresh weight. The relationship between fruits sunken-depth and fresh weight was highly significant(r=0.89** ). 7. The maximum of fruit pith was reached at late June. The maximum peak of the percentage of pith to total fruit weight was coincided with the first peak of fruit fresh weight growth rate at late June. At harvest, the percentage of pith to total fruit weight was only 3.6%. 8. About 58% of the total fruit drop were observed August 10-25 in control. Fruit removal force(FRF) was decreased and rate of fruit drop increased gradually as fruits were mature. The decrease of FRF was slow down and 0.91 kg at harvesting in control. Experiment II. Determination of formation and development pattern of abscission layer. 1. About 86% of pre-harvest drop "Yumyeong" peach fruits were occurred by separation at the junction of fruit and fruit stalk, 58, 76, 63 and 100% by 2, 4, 5 - TP 25 ppm, 50 ppm, 2, 4, 5-TP+ GA and ethephon respectively. 2. Formation of abscission layer was already differentiated at late July. The layer was formed the several layer of cell peripherally from outer cortex inward a t the junction of fruit and fruit stalk, and then the abscission layer developed at early August. Drop of fruit from fruit stalk might occur by separation according to middle lamella of intact cell wall. or by a mechanical fructuring of the vascular strands, and dissolution of the middle lamella followed by a collapse of the cells. Experiment III. Effects of growth regulatons on fruit growth and pre- harvest drop. 1. Ethephon spray increased fruit drop and more than 74% of total fruit drop were observed within 10 days after treatment. But 2, 4, 5-TP spray decreased fruit drop and marked only 12.3-26.4% at that time. 2. The FRF was sharply decreased by ethephon treatment, but it was much slowly decreased by 2, 4, 5-TP or 2, 4, 5-TP + GA treatments and over 1 kg at harvesting. A highly significant negative correlation was found between FRF and accumulative rate of fruits drop(r=-0.919 **). 3. Chemical treatments resulted in increased in fruit color developed, but decreased in fruit firmness. The relationship between fruit color and firmness was a significantly negative (r=-0.857**). Chemical treatments also proceeded maturation early, thus enable to harvest at the middle August. Especially, 2, 4, 5-TP 50 ppm spray resulted in better fruit color, however it resulted in lower fruit firmness, and occurred the symptom of dieback. Also severe drop of fruits and gummosis were observed by ethephon spray. 4. Treatments of 2, 4, 5-TP and 2,4, 5-TP+ GA increased the growth velocity of diameter and fresh weight of fruit, as compared to control. 5. Cells around exocarp in fruit flesh tissue began to show the swollen cell from 3 days after chemical sprays and became elliptical giant cells within 10-15 days, however similar elliptical giant cell were only found at harvesting period (late August) in control. 6. The Ca, Mg, and pectin contents in fruit were lower in chemical treatments than in control and were gradually decreased as fruits were mature. A significant negative correlation(r=-0.606*) was found between Ca content in fruit and accumulative rate of fruits drop. Experiment IV. Measurement of enzyme activities, ethylene and ABA content. 1. Peroxidase activity was increased by ethephon spray, or as fruits were mature,and decreased by 2, 4, 5-TP spray. Similar pattern was observed in pectinase activity. The peroxidase and pectinase activity, and accumulative rate of fruit drop showed a significant relationship(r=O.785**, r=0.703 *). 2. Ethylene production in ethephon treatment and control at severe drop period of fruits( early-middle August) was 0.58-1.82 and 0.75nmol /50g/hr. respectively. The ethylene production and accumulative rate of fruits drop showed a significant relationship (r=0.654*). 3. The peak of ethylene production and CO₂respiration was observed at August 18. But that of N₂O released was observed at August 25. A significant positive correlation (r=0.51**. r=0.41 **) was found between CO₂as well as N₂O evolution at respiration and ethylene production. 4. ABA content in "Yumyeong" fruits was the range from 0.18 ppm to 1.85 ppm, lower at early mature, and reached to peak at August 18. 5. A significant positive correlation was not found between ABA content and ethylene production in fruit. But the period of maximum ABA production coincided with period of heavy drop in fruit population. ABA content in fruits was increased directly after spray by ethephon.

‘Romance’ 스프레이국의 절화후 전ㆍ후처리가 저장성 및 절화수명에 미치는 영향

송남현(Nam Hyun Song),이선화(Sun Hwa Lee),서정근(Jung Keun Suh) 한국원예학회 1997 한국원예학회 학술발표요지 Vol.15 No.1

遮光程度와 入室時期가 改良映山紅의 生育 및 開花에 미치는 影響

宋南顯(Song Nam Hhyun),宋天永(Song Cheon Young),申東起(Shin Dong Gi) 한국원예학회 1989 한국원예학회 학술발표요지 Vol.7 No.1

복숭아 유명품종의 과실생장에 따른 생리해부학적 연구

송남현(N . H . Song) 원광대학교 생명자원과학연구소 1986 농대논문집 Vol.9 No.-

들깨 개화장해 한계 조도구명

이희덕,송남현,서관석,신복우,김홍규 한국작물학회 1989 한국작물학회 학술발표대회 논문집 Vol.1989 No.-

인삼 육성계통 캘러스부터 Polyacetylene의 분석 및 생합성에 미치는 배양조건

양덕춘,송남현,양계진,배창휴 한국식물생명공학회 2001 식물생명공학회지 Vol.28 No.3

인삼 callus의 기내배양에 의해서 항암물질을 대량생산하기 위한 연구의 일환으로 인삼 우수계통으로부터 기내에서 callus를 유기하여 polyacetylene 고함유 세포주를 선발하고, 인삼 callus의 polyacetylene 생산에 미치는 식물호르몬 및 배지의 영향을 조사하였다. 식물호르몬 CPA가 첨가된 MS배지에서 유도된 인삼 우수계통 callus에서는 polyacetylene 중에서 panaxynol은 TLC와 GC에 의해서 전혀 검출할 수 없었으나, callus에 따라서는 panaxydol이 형성됨을 확인할 수 있었다. 특히, 강력한 항암효과를 가지고 있는 panaxydol은 우수계통 callus 18종 중에서 5종이 형성하였으며, 30201계통 callus에서 가장 많이 검출되었다. 또한 인삼 10301계통 callus는 CPA가 단독으로 첨가된 배지에서 panaxydol을 생성하지 못하였으나, CPA 2 mg/L와 BA 0.05 mg/L 첨가구에서는 callus의 생장도 양호하였으며, panaxydol도 생성하였다. 인삼 callus의 생장과 panaxydol의 합성에는 MS배지보다 SH 배지가 더 양호하였으며, NAA와 sucrose는 전혀 영향을 미치지 않았다. In order to develop the biotechnological methods for the mass production of anticancer compounds from tissue culture of Panax ginseng C.A. Mayer, these studies were carried out for the selection of ginseng cell lines containing higher concentration of polyacetylene compounds and optimal condition for their biosynthesis. Panaxynol, one of ginseng polyacetylene, was not detected in any callus induced from ginseng superior cell lines cultured on MS medium supplemented with $\beta$-chlorophenoxy acetic acid (CPA). Panaxydol, another one of polyacetylene and anticancer compounds, were detected in calli of 5 cell lines by thin layer chromatogram and gas chromatogram. Among the 18 ginseng superior lines, the cell line 30201 has higher content of panaxydol. Especially, panaxydol was not detected in the callus induced from cell line 10301 which cultured on the medium containing CPA only, however, it was detected on the same callus cultured on mixed medium containing CAP 2 mg/L and BA 0.05 mg/L. SH medium was better than MS medium for ginseng callus growth and biosynthesis of polyacetylene, and also found that it was not effected by NAA and sucrose concentration in the culture medium.

질소시용량 및 재배양식이 대맥의 생육 및 수량에 미치는 영향

이희덕,이주열,송남현,채제천,조장환,김봉구 한국작물학회 1988 한국작물학회 학술발표대회 논문집 Vol.1988 No.-

오이 캘러스 분화시 광의 영향, 형태발생 및 단백질함량

이은모,조만현,송남현,우인식,이영복,곽상수 한국식물생명공학회 2000 식물생명공학회지 Vol.27 No.3

기내에서 무균적으로 생장시킨 오이 유묘의 경단조직과 하배축을 배양하여 캘러스 분화 및 생장에 대해 조사했다. 오이 경단조직을 생장조절물질이 첨가하지 않은 배지에서는 유식 물체로 분화하였으나 2,4-D가 0.5∼2 mg/L 첨가된 MS배지에서는 비배발생캘러스와 점액성캘러스가 유도되었고 배발생 캘러스는 분화되지 않았다. 그러나 유묘 육성시 암조건에서 발아 후 5∼7일 된 개체의 하배축을 5∼10mm크기로 절단하여 2,4-D가 1.0 mg/L이 첨가된 MS배지에 50일간 광조건에서 배양했을 때에는 배발생캘러스와 배상체가 분화되었다. 아울러 하배축 유래 비배발생캘러스 중 백색캘러스는 45.1%가 발근된 반면에, 노랑색캘러스는 불과 0.6%만 발근되었으며. 조단백질의 함량은 백색캘러스보다 노랑색캘러스가 월등히 많았다. 배발생캘러스와 배상체에서 발달된 소식물체는 순화과정을 거쳐 비닐하우스 내의 고형배지경에서 양액재배로 정상적인 과실을 수확하였다. To investigate the cucumber regeneration from embryogenic calli, shoot tips of aseptically-grown cucumber seedlings were used as explants for establishing tissue cultures. Growth and differentiation of callus were studied by using Murashige and Skoog's (MS) medium containing 0.5 to 2 mg/L 2,4-D. Plantlets were induced from shoot tip culture on the plant growth regulators-free MS medium. Non-embryogenic calli and viscous calli were induced on the medium supplemented with 0.5 to 2 mg/L 2,4-D, but embryogenic callus was not induced on the same medium. Segments (ca. 5∼10 mm) of aseptically-grown hypocotyl from five to seven days old seedlings after germination were placed on MS medium supplemented with 1 mg/L 2,4-D for 50 days. Embryogenic calli and embryoids were induced only from the seedlings grown in dark condition, and hypocotyl was placed on the media explanted in light condition. Foully-five point one percent of white fragile calli and 0.6% yellowish compact calli formed roots. Yellowish callus lines were investigated to have a considerably higher concentration of crude proteins than white callus lines. Plantlets derived from embryogenic calli or embryoids have been transferred to pots containing sterile vermiculite and perlite. Normal fruits were harvested from nutrient culture on aggregated hydroponics in the F-clean house.

수도에 대한 완효성비료 METAP의 비효시험(1972)

이은웅,안수봉,이문희,이주열,송남현,권순목,김칠용,최병초,정현식,조병옥,이일주,박창서,고춘산,박건호,정성채,정환수,이희덕,박성태,이한생,최승락 韓國作物學會 1973 한국작물학회지 Vol.- No.13

완효성 비료로서 METAP이 수도의 생육 수량 및 그 구성요소에 미치는 영향을 검토하고 그 효과를 단비와 비교하고자 농촌진흥청산하 3개작물시험장과 7개 농촌진흥원에서 동일한 설계하에서 일련의 시험을 실시하였다. METAP의 효과는 토양의 물리, 화학적 성질, 시용시기, 기상조건, 품종 및 재배법에 따라서도 다른 것 같으며 각 시험장에서 얻은 결과를 요약하면 다음과 같다. 1. 사질토에서 METAP의 시용은 수수를 증가시켜 수량이 증가하였으며 분시가 전량기비시용보다 효과가 좋았다. 그러나 해성토, 배수불량한 토양 및 비옥한 토양에서는 단비시용과 차이가 없었다. 2. 등숙에 요하는 온도가 높고, 조식에서 다수성인 품종(통일)을 비식하고 출수전후에 일조가 부족하고 온도가 낮으면 METAP시용이 등숙률 향상이나 1,000 입중을 증가시키지 못한다. Experiments were carried out to study the effectiveness of METAP as a slow released fertilizer on the growth, grain yield and its components of paddy rice, and to compare its effects with those of single fertilizers in the fields of 3 Crop Experiment Stations and 7 provincial Offices of Rural Development. The effectiveness of METAP seems to be different according to the physical and chemical characteristics of soil, the time of application, climatic conditions, variety and cultural methods. Therefore, the experimental results obtained from the above experiment are summurized as follows: 1. When METAP was applied into sandy soil, grain yield was increased due to increase of the number of panicles, and split application of METAP was more effective than basal only. However, in the reclaimed soil, not heavily percolated soil and fertile soil, no differences were observed between MET AP and single fertilizer applied plot. 2. When a rice variety, 'TONGIL' which is sensitive to the low temperature and produce higher yield in the early transplanting, was not transplanted early and air temperature is relatively lower during the ripening stage, the percentage of ripened grains and 1,000 grains weight were not higher in the METAP applied plots than in the single fertilizer applied plots.