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Regulation of Adipocyte Differentiation via MicroRNAs
손유화,가소정,김재범 대한내분비학회 2014 Endocrinology and metabolism Vol.29 No.2
Adipocyte differentiation, termed adipogenesis, is a complicated process in which pluripotent mesenchymal stem cells differentiate into mature adipocytes. The process of adipocyte differentiation is tightly regulated by a number of transcription factors, hormones and signaling pathway molecules. Recent studies have demonstrated that microRNAs, which belong to small noncoding RNA species, are also involved in adipocyte differentiation. In vivo and in vitro studies have revealed that various microRNAs affect adipogenesis by targeting several adipogenic transcription factors and key signaling molecules. In this review, we will summarize the roles of microRNAs in adipogenesis and their target genes associated with each stage of adipocyte differentiation.
다중회귀분석을 이용한 원전해체 방사성폐기물 발생량 예측 식 개발에 관한 연구
손유화,하재철,조천형 (사)한국방사선산업학회 2021 방사선산업학회지 Vol.15 No.4
In order to reduce the cost of radioactive waste disposal and to manage it efficiently,it is necessary to estimate the amount of radioactive decommissioning waste from nuclear powerplant. In this paper a predictive model was developed by a multi-regression analysis using Rstatistics package with reference to previous research. Decommissioning waste amount data for12 pressurized water reactors, 4 pressurized heavy water reactors and 3 boiling water reactorswere collected for a sample of multi-regression model. In regression analysis, amount of estimatedradioactive decommissioning waste was set as the dependent variable, and design net capacity,effective age, decommissioning strategy were selected as independent variables through threevariable selection methods. Test statistics: determination coefficient, adjusted determinationcoefficient, p-value, variance inflation factor of regression analysis were checked to verifysignificance of derived predictive model. It is expected that a more accurate predictive model canbe developed by obtaining additional decommissioning data of nuclear power plants.
Pharmacokinetic characterization of CK2 inhibitor CX-4945
손유화,송진숙,김성환,김지연 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.7
Over-expression of protein kinase CK2 ishighly linked to the survival of cancer cells and the poorprognosis of patients with cancers. CX-4945, a potent andselective orally bioavailable ATP-competitive inhibitor ofCK2, inhibits the oncogenic cellular events such as proliferationand angiogenesis, and the increase of tumorgrowth in mouse xenograft model. In this study, thepharmacokinetic information about CX-4945 was provided;at 10 lM, CX-4945 with high stability in human andrat liver microsome exhibited low percentage of inhibition(\10 %) in CYP450 isoforms (1A2, 2C19, 3A4), butconsiderable inhibition (*70 %) in CYP450 2C9 and 2D6. In hERG potassium channel inhibition assay, CX-4945exhibited relatively low inhibition rate. Additionally,CX-4945 showed high MDCK cell permeability ([10 910-6 cm/s) and above 98 % of plasma protein binding inthe rat. After intravenous administration, Vss (1.39 l/kg)and extremely low CL (0.08 l/kg/h) were observed. Moreover, orally administrated CX-4945 showed highbioavailability ([70 %) and these data might be related tothe MDCK cell permeability results.
The Protein Kinase 2 Inhibitor CX-4945 Regulates Osteoclast and Osteoblast Differentiation In Vitro
손유화,김지연,문성희 한국분자세포생물학회 2013 Molecules and cells Vol.36 No.5
Drug repositioning can identify new therapeutic applica-tions for existing drugs, thus mitigating high R&D costs. The Protein kinase 2 (CK2) inhibitor CX-4945 regulates human cancer cell survival and angiogenesis. Here we found that CX-4945 significantly inhibited the RANKL-indu-ced osteoclast differentiation, but enhanced the BMP2-induced osteoblast differentiation in a cell culture model. CX-4945 inhibited the RANKL-induced activation of TRAP and NFATc1 expression accompanied with sup-pression of Akt phosphorylation, but, in contrast, it enhanced the BMP2-mediated ALP induction and MAPK ERK1/2 phosphorylation. CX-4945 is thus a novel drug candidate for bone-related disorders such as osteoporosis.
시스템 다이내믹스를 이용한 중 · 저준위 방사성폐기물 처분량예측 모델에 관한 연구
손유화,하재철,조천형 (사)한국방사선산업학회 2022 방사선산업학회지 Vol.16 No.4
Gyeongju radioactive waste repository is a complex disposal facility planned to dispose ofup to 800,000 drums of very low, low and intermediate level radioactive waste in Korea. This complexdisposal facility are composed of several different types of disposal facilities. In order to operate disposalfacility efficiently, it is necessary to establish a disposal plan considering the amount of radioactivewaste diposal. The amount of radioactive waste disposal can be predicted by analyzing the correlationbetween the amount of radioactive waste generated, temporary storage and disposal capacity. Thesystem dynamics modeling is one of suitable tools for correlation analyze and it is specialized in modelingof time dependent material flow. The research procedure was determined through the analysis ofpreceding studies about the prediction of the amount of radioactive waste disposal, and a predictionmodel for disposal amount of low and intermediate level waste was designed. As a result of predictivemodeling, it was predicted that the generation of radioactive waste would reach 742,064 drums at 2095and the optimized annual disposal amount would reach should be 12,436 drums. In addition, throughscenario analysis about nuclear power plant operation, it wast found that additional nuclear powerplant construction increases annual disposal amount, whereas extending nuclear power plant lifetimedecreases annual disposal amount.
Identification of Mitotic Blockers by Phenotypic Screening of a Clinical Compound Library
손유화,Chi Hoon Park,최상운 대한화학회 2016 Bulletin of the Korean Chemical Society Vol.37 No.12
A phenotypic assay was performed using a clinical compound library to identify the new anticancer agents. HeLa cells were treated with the clinical compounds for 20 h, and then screened for cell morphology to check the mitotic arrest. A total of 2320 clinical compounds were tested, of which two anthelmintic compounds, oxibendazole and oxfendazole, were found to induce mitotic arrest in HeLa cells. Additionally, cell-cycle analysis revealed strong G2/M arrest by these anthelmintics. Moreover, the two compounds, especially oxibendazole, exhibited highly cytotoxic effects on several cancer cell lines. In conclusion, we suggest repositioning of the two anthelmintics, oxibendazole and oxfendazole, if normal cells are not affected by these compounds, to cancer treatment.
3-Amino-1H-pyrazolopyridine Derivatives as a Maternal Embryonic Leucine Zipper Kinase Inhibitor
박철민,Vithal B. Jadhav,송종환,이선경,원희영,최상운,손유화 대한화학회 2017 Bulletin of the Korean Chemical Society Vol.38 No.6
Maternal embryonic leucine zipper kinase (MELK) has been implicated in various cellular processes and is highly upregulated in diverse cancers, then it is thought to be a promising anticancer target. 3-Anilino-1H-pyrazolo[3,4-b]pyridine scaffold was identified as a novel scaffold for MELK kinase inhibitors in high throughput screening (HTS). From exploration for structure–activity relationship (SAR) studies mainly by the modification of the 3 (C3), and 5-positions (C5), 4-(4-methylpiperazin-1-yl)-N-{5-[1-(piperidin-4-yl)-1H-pyrazol-4-yl]-1H-pyrazolo[3,4-b]pyridin-3-yl}benzamide (21a) was found to exhibit good activity (IC50 = 0.15 μM) on MELK as a lead in early state.