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손규희(Kyu Hee Son) 한국생태공학회 2016 한국생태공학회지 Vol.- No.S
The number of mud flat in Korea has been gradually decreased because of the coastal development occurred in last 40 years. However, the policy framework has been established with understanding of the values of marine environment and its ecosystem services. In particular, several mud flat restoration projects in 9 different sites including Suncheon, Sacheon and Gochang have been completed and also in process. The accomplishments produced from these projects assisted to develop technical guideline on restoration concepts, but found out limitations of legal frameworks supporting technical regulations, assessment of site designation and establishment of its objectives. Also, lack of monitoring system to assess the effects of restoration activities drives not to reach rational conclusions, extending the projects nationally. Therefore, it is needed to improve policy framework in order to extend restoration activities as well as understanding of restoration effects by following four points. Firstly, an item assessing effects should be added in selection process of mud flat projects. Secondly, a technical guideline should be in effective legally to follow. Thirdly, detailed roles and duties of stockholders including Ministry of Oceans and Fisheries, local authorities, research institutes, local residents and any industries involved should be described and considered. Fourthly, it is needed to differentiate the budgets depending on types and scales of restoration projects. By considering similar cases implemented inside and outside country, restoration projects applicable to our country will be carried out.
황복 , Takifugu obscurus , 초기 단계의 소화효소 변화
손규희(Kyu Hee Son),한경남(Kyung Nam Han),장정순(Chung Soon Chang) 한국수산과학회 2001 한국수산과학회지 Vol.34 No.6
황복의 소화효소의 변화과정을 부화직후부터 부화 후 65일까지 측정하였다. 발육단계에 따른 소화효소의 발현과 활성의 변화에서 α-amylase 비활성은 전장 10 ㎜에서 0.0493 U/㎎의 최소값을 나타낸 후, 전장 19 ㎜를 전후하여 0.1480 U/㎎의 최대값을 나타냈다. Trypsin과 pepsin 비활성은 전장 16 ㎜에서 각각 0.0264 U/㎎, 0.0258 U/㎎ 와 전장 24 ㎜에서 0.0178 U/㎎, 0.0201 U/㎎의 값을 가지는 두 번의 peak를 보였고, 이 시기에 황복 자치어의 성장률도 증가하는 경향을 보였다. 또한, trypsin과 pepsin의 비활성을 비교하여 보면, 자어기인 전장 4∼5 ㎜와 치어 II기인 전장 19∼24 ㎜에서는 pepsin이 높았고, 치어 I 기인 전장 11∼16 ㎜와 유어기인 전장 27 ㎜ 이후에서는 trypsin이 높았다. The digestive enzyme activities such as α-amylase, trypsin and pepsin from the laboratory-reared river puffer, Takifugu obscurus, were measured from the time course of 1 day until the 65 day after hatching. In the case of α-amylase, it was showed minimum activity of 0.0493 U/㎎ at the total length (TL) 10 ㎜, and showed maximum activity of 0.1480 U/㎎ at 19 ㎜TL. Trypsin and pepsin were showed their maximum activities of 0.0264, 0.0258 U/㎎ and 0.0178, 0.0201 U/㎎ when the total length of 16 and 24 ㎜, and represented remarkable correlations between the changes of enzyme activity and growth rate. The ontogenetic variations of digestive enzymes were represented clearly different patterns; i.e. the pepsin showed higher activity when the periods of larva (4∼5 ㎜TL) and juvenile II (19∼24 ㎜TL), however, the trypsin represented maximum activity at the stages of juvenile I (11∼16 ㎜TL) and young fish (27 ㎜TL), respectively.
닭 도축장에서 분리한 nalidixic acid 내성 Salmonella 균의 gyrA 유전자 돌연변이
조재근,손규희,김경희,김정미,박대현,이정우,Cho, Jae-Keun,Son, Kyu-Hee,Kim, Kyung-Hee,Kim, Jeong-Mi,Park, Dae-Hyun,Lee, Jung-Woo 한국동물위생학회 2019 韓國家畜衛生學會誌 Vol.42 No.3
The objective of this study was to identify mutations in the quinolone resistance determining region (QRDR) of the gyrA, gyrB, parC and parE genes, and the presence of plasmid-mediated quinolone resistance (PMQR) genes: qnrA, qnrB, qnrS, aac(6')-lb-cr and qepA in 40 nalidixic acid- resistant ($NA^R$) Salmonella isolates isolated from poultry slaughterhouse. The MIC of NA and ciprofloxacin for 40 $NA^R$ Salmonella isolates was $128{\sim}512{\mu}g/mL$ and < $0.125{\sim}0.25{\mu}g/mL$, respectively. The Salmonella isolates were resistant to NA (100%), gentamicin (5.0%) and ampicillin (2.5%). All $NA^R$ Salmonella isolates represented point mutation in codons Aspartic acid(Asp)-87 (90%) and Serine(Ser)-83 (10%) of QRDR of gyrA gene: $Asp87{\rightarrow}glycine$, $Ser83{\rightarrow}tyrosine$. No mutations were observed in QRDR of the gyrB, parC and parE gene. Moreover PMQR genes was not found in any of the tested isolates. Our findings showed that DNA gyrase is the primary target of quinolone resistance and a single mutation in codon Asp87 and Ser83 of the gyrA gene can confer resistance to NA and reduced susceptibility ciprofloxacin in Salmonella isolates.