치성 각화낭종의 임상적 연구

성화식(Hwa-Sik Seong),이주민(Ju-Min Lee),황대석(Dae-Seok Hwang),김용덕(Yong-Deok Kim),김욱규(Uk-kyu Kim),김종렬(Jong-Ryoul Kim),정인교(In-Kyo Chung),신상훈(Sang-Hun Shin) 대한구강악안면외과학회 2009 대한구강악안면외과학회지 Vol.35 No.2

Purpose: The purpose of this study is to investigate the clinical and histological features of odontogenic keratocyst Patients and Methods: A retrosective review of 100 patients who were diagnosed as odontogenic keratocyst by hitological findings during the period of January 2000 and December 2005 in the Dept. of Oral and Maxillofacial surgery Pusan National University was consecuted. For each patient, age, sex, location of lesion, initial diagnosis by radiographic features, treatment procedure, hitologic findings and recurrance rate were evaluated. Results: In this study, OKC has male prevalance to female by 1.38:1, and most likely occurs during third decade. The most common site of lesion was mandibular ramus region(34.6%) and the most common symptom was swelling(50%). The most common initial diagnosis by radiographic findings was OKC and cyst enucleation was the most common treatment method. The recurrance rate was 28% and existence of daugther cyst is thought to be most convincing factor for prediction of recurrence. Conclusion: In this study, total recurrence rate was 28% and existence of daugther cyst is thought to be most convincing factor for prediction of recurrence. But, since 97% of patients were treated by enucleation and adjuntive excision, further styudy is need about concordance of recurrence rate with surgical method.

Recombinant human bone morphogenic protein-2 Induces the Differentiation and Mineralization of Osteoblastic Cells Under Hypoxic Conditions via Activation of Protein Kinase D and p38 Mitogen-Activated Protein Kinase Signaling Pathways

하우훈,성화식,최나래,박봉수,김용덕 한국조직공학과 재생의학회 2017 조직공학과 재생의학 Vol.14 No.4

Hypoxia suppresses osteoblastic differentiation and the bone-forming capacity. As the leading osteoinductive growth factor used clinically in bone-related regenerative medicine, recombinant human bone morphogenic protein-2 (rhBMP- 2) has yielded promising results in unfavorable hypoxic clinical situations. Although many studies have examined the effects of rhBMP-2 on osteoblastic differentiation, mineralization and the related signaling pathways, those of rhBMP-2 on osteoblastic cells remain unknown, particularly under hypoxic conditions. Therefore, this study was conducted under a 1% oxygen tension to examine the differentiating effects of rhBMP-2 on osteoblastic cells under hypoxia. rhBMP-2 could also induce the differentiation and mineralization of Osteoblastic (MC3T3-E1) cells under1%hypoxic conditions. rhBMP-2 could also induce the differentiation and mineralization of MC3T3-E1 cells under 1% hypoxic conditions. rhBMP-2 increased the alkaline phosphatase {ALP} activity in a time dependent manner, and expression of ALP, collagen type-1 (Col-1) and osteocalcin (OC) mRNAwere up-regulated significantly in a time- and concentration-dependent manner. In addition, the area of the mineralized nodules increased gradually in a concentration-dependent manner. Western blot analysis, which was performed to identify the signaling pathways underlying rhBMP-2-induced osteoblastic differentiation under hypoxic conditions, showed that rhBMP-2 significantly promoted the phosphorylation of the p38 mitogen-activated protein kinase (MAPK) in a time-dependent manner. A pretreatment with SB203580, a p38 MAPK inhibitor, inhibited the rhBMP-2-mediated differentiation and mineralization. Moreover, the phosphorylation of p38 induced by rhBMP-2 was inhibited in response to a pretreatment of the cells with Go6976, a protein kinase D {PKD) inhibitor. These findings suggest that rhBMP-2 induces the differentiation and mineralization of MC3T3-E1 cells under hypoxic conditions via activation of the PKD and p38 MAPK signaling pathways.

가토의 측두하악관절원판 결손에서 간세포 성장인자가 치유에 미치는 영향

김복주,성화식,김철훈,김규천,황희성,신상훈,Kim, Bok-Joo,Seong, Hwa-Sik,Kim, Chul-Hoon,Kim, Gyoo-Cheon,Hwang, Hee-Sung,Shin, Sang-Hun 대한악안면성형재건외과학회 2009 Maxillofacial Plastic Reconstructive Surgery Vol.31 No.1

Purpose: The purpose of this study is to investigate the therapeutic use of Hepatocyte growth factor(Adv.CMV.HGF) in temporomandibular joint disc defect. Materials and methods: Twelve New Zealand white rabbits, weighing 2.5 - 3.0 kg, were used in this experiment. Defects(2 mm in diameter) were created in their TMJ discs. Recombinant Adv.CMV.HGF with gelatin sponge($Gelfoam^{(R)}$) as carrier was implanted in the defects. We divided the rabbits into four batches according to the duration of the implantation - of 1, 4, 8, 12 weeks - and both left and right TMJ of each rabbit in all groups were used in the research : left joints were used as experiment group and right were control group. Each batch of rabbits was killed one, four, eight and twelve weeks after the experimentation respectively, and called Group A, B, C, and D. (Group A = 1 wk, B = 4 wks, C = 8 wks, and D = 12 wks) Results: The experimental group showed a significant increase in the number of chondroblasts and active cell differentiation at the margin of the defects. Compared to the control group, in the experiment group chondroblasts increased and chondrocytes showed a columnar arrangement, which is witnessed at the time of cell differentiation. Conclusion: This study supports the case that Avd.CMV.HGF may be useful in the repair of articular disc of the rabbit TMJ.

각화낭성치성종양의 재발과 연관된 p53, Ki-67, PCNA 및 cytokeratin 17, cytokeratin 18의 발현

신상훈,성화식,이승환 부산대학교 병원 암연구소 2008 부산대병원학술지 Vol.- No.24

The keratocystic odontogenic tumor(OKC) represents approximately 11% of odontogenic cysts and is decided by microscopic and histopathologic determinant rather than by clinical apprearance. The OKC is noted for its capacity to recur after treatment. In this study, p53, Ki-67, PCNA and cytokeratin 17, cytokeratin 18 expression of OKC in relation to non-recurrenct lesion and recurrent lesion after cyst enucleation were examinated. The p53, Ki-67, PCNA and cytokeratin 17, cytokeratin 18 antibody staining were used. The obtained results were as follows. In proliferating rate of non-recurrent OKCs and recurrent OKCs using p53, Ki-67 and PCNA staing, there was not significant difference. In cytokeratin 17 staining, strong positive reaction was showed in all layers of recurrent OKCs rather than non-recurrent OKCs. In cytokeratin 18 staining, negative or weakly positive reaction was showed in recurrent OKCs rather than non-recurrent OKCs. In summary, it was suggested that the recurrence of OKCs was not related to proliferating rate of epithelium and the prognosis of the recurrence of OKCs is helped using cytokeratin 17 and cytokeratin 18.

응급실을 내원한 구강악안면외과 안면골 골절 환자의 변화에 대한 비교 연구

신상훈,성화식,강영훈,황대석,김용덕,김욱규,김종렬,정인교 부산대학교 병원 암연구소 2008 부산대병원학술지 Vol.- No.24

Although there have been many clinical studies about the facial trauma in the recent as well as in the past, severity and frequency of facial trauma are reported multifarious. It seemed to be because of variety of social, cultural, and environmental factors, and the view point of investigators. In this study, we investigated about the patients visiting emergency room during recent 4 years(2004-2007), and compared with retrospective study during former 10 years(1992-1996, 1999-2003) in Dept. OMS Pusan National University Hospital. We assessed the cause, type, demographic, etc, and analyzed alterations of facial bone fractures during past 3 periods. The results were as follows. The total number of patients was increased. 429 patients treated for maxillofacial fractures between January 1992 and December 1996, 466 patients treated between January 1992 and December 1996 and 764 patients treated between January 2004 and December 2007. The male-to-female ratio were 4.6:1, 3.9:1, and 4.16:1, respectively. Patient in the age groups of 10-19 years and 20-29 years increased in the latter two periods. Assaults and falls are decreased gradually, but slip down s are increased. Traffic accidents are still major cause of facial trauma. The mandibular fractures showed the highest incidence thorought three periods. Symphysis fractures of mandible was increased and condyle fractures of mandible was drecreased in the third period.