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3-NP에 의해 유발된 신경교세포의 산화적 손상에 대한 남성(南星)의 보호효과
이정섭,신용진,전지영,설재균,최철원,신선호,이인,남상규,Lee, Jung-Sup,Shin, Yong-Jin,Jeon, Ji-Young,Seol, Jae-Gyun,Choi, Chul-Won,Shin, Sun-Ho,Lee, In,Nam, Sang-Kyu 대한한방내과학회 2007 大韓韓方內科學會誌 Vol.28 No.3
Objectives : This study aimed to investigate the underlying protective mechanism of Rhizoma Arisaematis(RA) on 3-NP-induced Cytotoxicity in rat C6 glial cells. Methods : We investigated treatment ofC6 cells with 20mM 3-NP and pretreatment with RA to cause loss of cell viability. and morphological change. which was associated with elevation of ROS level. increase in Bax/Bcl2 ratio and HIF-a protein expression Results : RA inhibited 3-NP-induced cell death in C6 glial cells and inhibited the changes of the : MMPT (mitochondria membrane potential transition) and inhibited the decrease of mitochondria complex II activity and 3-NP-induced ROS generation in C6 cells. And RA decreased the activity of HIF-a and Bax. and increased the activity of $Bcl_2$ in C6 glial cells Conclusions : RA markedly protects C6 glial cells from 3-NP-induced oxidative injury.
자금정(紫金錠)이 간암세포주 HepG2의 세포고사 및 세포주기에 미치는 영향
조영기,전지영,신용진,설재균,이재화,원진희,문구,Cho, Young-Kee,Jeon, Ji-Young,Shin, Yong-Jeen,Seol, Jae-Kyun,Rhee, Jae-Hwa,Won, Jin-Hee,Moon, Goo 대한한방내과학회 2007 大韓韓方內科學會誌 Vol.28 No.4
Objectives : Jageum-Jung is used as an anti-cancer agent in oriental medicine, but the mechanism by which it induces cell death in cancer cells is still unclear. The purpose of this study was to investigate the effects of Jageum-Jung on apoptosis and cell cycle arrest in HepG2 hepatoma cells. Methods : Various cancer cell lines including HepG2, C6 glioma, SH-SY5Y, PANC-1, and MCF-7 cells, were used. Apoptosis was determined by DAPI nuclei staining and flow cytometry in HepG2 cells treated with various concentrations (from 25 to 200 ${\mu}g/ml$) of $H_2O$ extract of Jageum-Jung (JGJ) for 48 hrs. Expression of cell cycle arrest mediators including Rb, p53, p21, cyclin B1, cdk4, and cyclin E proteins were measured by Western blot analysis. To estimate intracellular hydrogen peroxide levels and intracellular nitric oxide levels, HepG2 cells were stained with DCFH-DA dye and DAF dye, subjected on flow cytometric analysis. Results : 1. Jageum-Jung decreased the viability of HepG2 cells in a dose-dependent manner. 2. Jageum-Jung induced the catalytic activation of caspase-3 in HepG2 cells. 3. Jageum-Jung increased the intracellular hydrogen peroxide and NO in HepG2 cells. 4. Jageum-Jung increased the expression of Rb, p53 and p21 in HepG2 cells. 5. Jageum-Jung induced the expression of cyclin B1, cdk4, and cyclin E in HepG2 cells. Conclusions : Taken together, we suggest that Jageum-Jung exhibits cytotoxic effects on HepG2 cells, causing apoptosis and cell cycle arrest. The results showed that Jageum-Jung may do so by regulating the expression of specific target molecules that promote efficient apoptotic cell death following $G_2$/M phase arrest in a dose-dependent manner.