韓半島 南部地方에 分布된 毒草類와 人體에 나타나는 症勢에 關한 調査

서병부,서재욱,남기홍 대구대학교 기초과학연구소 1987 基礎科學硏究 Vol.4 No.-

The magnitude of human hazard from plant ingestion is suggested by the fact that plants are the second most frequent hazardous material ingested by children under 5 years of age and the fifth most frequent hazardous material ingested by humans of all ages. Common household and garden plants are among those most commonly ingested. A variety of potential clinical entities can be produced by most parts of these plants, including digestive tract disorders, cardiac irregularities, hallucinogenic effects, convulsions, and liver damage. There is much to be learned about the relationship between experimentally induced plant ingestion in animals and actual human exposure.

Development of Chronic Neutrophilic Leukemia

서병부,박흠대 사단법인 한국동물생명공학회 2011 Reproductive & developmental biology Vol.35 No.4

The experimental manipulation of protooncogenes and their gene products is a valuable research tool for the study of human neoplasia. In this study, the recently identified human cervical cancer protooncogene (HccR-2) was expressed in transgenic mice under the control of the tetracycline regulatory system. Mice expressing the HccR-2 transgene showed an altered myeloid development characterized by an increased percentage of mature and band-form neutrophils in the peripheral blood, liver and spleen. This phenotype is similar to human chronic neutrophilic leukemia (CNL) in many ways, which is a rare chronic myeloproliferative disorder (CMD) that presents as a sustained leukocytosis of mature neutrophils with a few or no circulating immature granulocytes, an absence of peripheral blood monocytosis, basophilia, or eosinophilia, and an infiltration of neutrophils into the liver, spleen and kidney. Thus, the HccR-2 transgenic mouse model is imperative not only for investigating the biological properties of the HccR-2protooncogene in vivo, but also for analyzing the mechanisms involved in the progression of CNL.

배양액 종류가 B6D2F1 마우스 배아발생능력에 미치는 영향

유창석,박기상,서병부 한국수정란이식학회 2016 한국동물생명공학회지 Vol.31 No.1

This study was conducted to evaluate the effects of type of culture media (BM, G2, OS, TCM, and MEM) on B6D2F1 mice oogenesis. In the present study, B6D2F1/CrljOri F1 mice were utilized in order to maximize oogenesis. Also we used TCM-199, Dulbecco's medified Eagle's medium (DMEM), embryo culture medium (Fertilization medium, Cleavage medium, Blastocyst medium), G series medium and One step medium. In vitro maturation was highest in BM followed by the order of OS, MEM, TCM and G2 (90±2.8% > 88±3.2% > 85±4.9% > 78±10.2% > 64±7.7%, respectively). To note, the G2 group was statistically different compared to other groups (p<0.05). On the other hand the fertilization rate was highest in the G2 group followed by BM, OS, TCM, and MEM (87±7.2% > 85±6.9% > 74±14.0% > 71±13.8% > 2±1.4%, respectively). The MEM group was significantly lower compared to other groups (p<0.05). The developmental rate was highest in the OS group followed by the G2 group and the BM group albeit no statistical significance was noted (73±11.6% > 71±9.2% > 66±10.4%). Of note, all cells of the TCM and MEM groups were died during embryonic development. The zona hatched rate (51±9.8% vs. 50±9.1% vs. 47±7.2% for BM, G2, and OS respectively) and attached rate (45±12.3% vs. 38±16.1% vs. 37±11.5% for BM, G2, and OS respectively) were not different amongst groups. No difference was found in total cell numbers (74±13.9 vs. 64±9.2 vs. 76±6.7 for BM, G2, and OS respectively), ICM cell numbers (20±1.9 vs. 14±1.8 vs. 15±2.1), TE cell numbers (55±12.5 vs. 49±10.7 vs. 61±5.9), % ICM (30±2.8% vs. 24±7.0% vs. 22.8±2.2%) and ICM:TE ratio (1:2±0.5 vs. 1:3.1±0.8 vs. 1:3.1 ±0.5) amongst groups. In summary, these results can provide fundamental data to maximize culture condition for in vitro fertilization on B6D2F1 mice.

동물매개치료가 자폐스펙트럼장애아동의 대인관계, 언어 및 행동에 미치는 사례연구

정순미(Jung, Soon-Mi),서병부(Seo, Byoung-Boo),김충희(Kim, Chung-Hui) 한국보건기초의학회 2020 한국보건기초의학회지 Vol.13 No.2

This study is a single case study that analyzed the effects of animal-assisted therapy (AAT) on a 5-years-old male child diagnosed with autism spectrum disorder class II. To verify the analysis, the Korean Childhood Autism Rating Scale Second Edition (K-SCAR 2), which measures the degree of autism, and the EHWA – Check List for Autistic Children (E-CLAC) scale, which is a behavioral development evaluation scale, were used. The AAT program consisted of a total of 12 sessions, for 50 minutes per session, and was carried out once a week for 12 weeks. As a result of the therapy, the autistic child showed increases in the frequency of word use along with increased language use and behavioral interactions. A K-SCAR 2 test was carried out and the results indicated that the child’s autistic condition had improved from severe autism to moderate autism bordering on minor autism. The E-CLAC indicated that the ability of the child to socially interact had improved as his human relationships were improved and the frequency of language use had increased. In conclusion, the results of this experiment indicated that when AAT was implemented with a child with autism spectrum disorder, the level of autism was lowered, while his human relationships improved as well as his language and use behavioral interactions.

배양액 용량이 B6D2F1 마우스 배아발생능력에 미치는 영향

유창석,박기상,서병부 한국수정란이식학회 2016 한국동물생명공학회지 Vol.31 No.1

This study was conducted to evaluate the effects of different volume (100 μl vs. 2 ml) of microdrop culture on B6D2F1 mice oogenesis. In the present study, B6D2F1/CrljOri F1 mice were utilized in order to maximize oogenesis. Also we used TCM-199, Dulbecco's medified Eagle's medium (DMEM), embryo culture medium (Fertilization medium, Cleavage medium, Blastocyst medium), G series medium and One step medium. Blastulation rate was not different between groups (58.4±2.9% vs. 61.2±4.8%). Zona hatched rate (38±15.4% vs. 27±3.4%) and attached rate (55±13.9% vs. 46±3.9%) did not differ by the volume of culture media. Total cell numbers (59.8±9.7 vs. 70.3±8.7), ICM cell numbers (15.8±0.6 vs. 16.8±1.5), TE cell numbers (44.0±9.7 vs. 53.6±7.3), % ICM (26.4±2.9% vs. 23.8±3.3%) and ICM:TE ratio (1: 2.8±0.4 vs. 1: 3.2±0.6) were not different between groups (i.e., 100 μl vs. 2 ml). These results show that the capacity of the culture medium did not effect the cell numbers of B6D2F1 mice blastocysts. In summary, these results can provide fundamental data to maximize culture condition for in vitro fertilization on B6D2F1 mice.

Sperm motility and viability of experimental animals using different cryopreservatives

박원용,서병부 사단법인 한국동물생명공학회 2023 Journal of Animal Reproduction and Biotechnology Vol.38 No.1

In this study, an experiment was conducted in order to determine what cryopreservatives (CPVs) were more effective in supporting the motility and viability of sperm from experimental animals. The sperm of mice, rats, beagle dogs, and rabbits were frozen using different CPVs, including DMSO, TYB, and Sperm CryoProtec. The results from freezing the sperm of each laboratory animal in Sperm CryoProtec showed a high level of sperm motility and viability in sperm samples from mice, rats, and beagle dogs melted at the end of the first week. For rabbits, a high level of motility was observed in sperm thawed during the first week, whereas a high level of viability was observed in sperm thawed during the second week. The results of analysis of sperm motility and viability using different CPVs according to laboratory animals showed a significantly higher level of sperm motility (26.28%) and viability (36.20%) for mice in Sperm CryoProtec and the lowest levels of motility and viability were observed in DMSO (p < 0.05). Significantly higher levels of motility (27.94%) and viability (37.94%) were observed for rats in Sperm CryoProtec compared with TYB, which showed the lowest levels of motility and viability (p < 0.05). The study findings described above suggest that the selection of appropriate cryopreservatives is required for each experimental animal. This is because there are differences in the levels of sperm motility and viability of experimental animals depending on the CPVs that are typically used for freezing human sperm, including Sperm CryoProtec and TYB.

Mousr 난자의 체외배양과 체외수정에 있어서 Medium에 첨가하는 Calcium Chelate 물질의 효과

박기상,서병부,김광식,송해범 大邱大學校出版部 (대구대학, 한사대학 1993 農業科學硏究論文集 Vol.7 No.-

This research was conducted to comparison of the influences of the calcium chelate added in media on oocyte maturation and in vitro fertilization using out-bred ICR mice. The results obtained are as follows, 1. In vitro maturation rates were 42%, 33%, 36%, 51%, 55%, 53%, 54% and 34% when the medium was added with EDTA, EGTA, PMA, PMA-M, PHA-P, Vit-A, Butyric-acid and control, respectively. EDTA, PHA-M, PHA-P, Vit-A and Butyric-acid were the best among media. 2. In vitro fertilization rates were 80%, 74%, 37%, 53%, 83%, 35%, 84% and 81% when the medium was added with EDTA, EGTA, PMA, PHA-M, PHA-P, Vit-A, Butyric-acid and control, respectively. PHA-P and Butyric-acid were the best among the media.

한우 수정란이식에 의한 쌍자 생산

이준영(Jun Young Lee),정연길(Yun Gil Jung),서병부(Byoung Boo Seo) 한국동물보건학회 2023 한국동물보건학회지 Vol.2 No.3

Mouse의 연령과 체중이 배란율, 핵성숙도 및 체외수정에 미치는 영향

박기상,서병부,송해범 大邱大學校出版部 (대구대학, 한사대학 1992 農業科學硏究論文集 Vol.6 No.-

This research was conducted to investigate the interrelationship among age and body weight of mice to the number of ovulated eggs, percentage of matured oocytes and in vitro fertilization using out-bred ICR mice. The results obtained are as folows, 1) The number of ovulated eggs was 40±8~42±7, percentage of MII was 75~76% and in vitro fertilization rate was 79~81% in 3~6 weeks age of mice. 2) The number of ovulated eggs were 39±8 and 41±7, percentages of MII were 75 and 76% and in vitro fertilization rates were 81 and 80% in 15 and 25g weight of mice, respectively.

다양한 유리화 동결 방법이 각 시간대별 생쥐 전핵기 배아의 발달에 미치는 영향

김지철,서병부,박성백,김재명 韓國受精卵移植學會 2012 한국동물생명공학회지 Vol.27 No.1

The objective of this study was to investigate the effectiveness of cryopreservation methods for the effect of various vitrification containers, such as EM-grid, OPS, or cryo-loop on the survival and developmental rate of vitrified mouse pronuclear embryos, and mouse cleavage embryo, at 21, 24, 27 and 30 hr after hCG injection. Post-thaw cleavage was similar among treatments, while the developmental rates of mouse blastocyst and hatched blastocyst were higher ( <0.05) in 27 hr and 30 hr than 21 hr. The developmental rate of hatched blastocyst at vitrified cleavage mouse embryos in cryo-loop was significantly higher than vitrified pronuclear embryos of control group as well as EM-grid and OPS ( <0.05). The developmental rate using cryo-loop was higher than EM-grid, but in case of OPS at vitrified cleavage and mouse pronuclear embryos, no significant difference was noticed. These results of our study show that the developmental rates of mouse embryos were unaffected by various vitrification containers, but in case of mouse embryos and hatched blastocysts at late vitrified pronuclear embryos the developmental rates were higher than early vitrified pronuclear embryos. Moreover, the developmental rate of hatched blastocyst at vitrified cleavage mouse embryos was significantly higher than vitrified pronuclear embryos. For better execution of this study, it will be mandatory to include improvement of vitrification containers, cryopreservation methods and conditions, higher survival rate, safe preservation, contamination and embryo loss.