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초청 총설 : 바이오에너지 및 바이오화학원료인 C4-C6 생산
김병천 ( Byung Chun Kim ),이성철 ( Sung Chul Yi ),상병인 ( Byoung In Sang ) 한국공업화학회 2011 공업화학 Vol.22 No.5
석유자원의 고갈이 에너지 및 화학원료물질로 재생 가능한 바이오매스의 이용성을 증가시키고 있다. 본 총설에서는 바이오에너지 및 바이오화학원료인 C4-C6 생산에 관해 논하고자 한다. 주요한 C4 물질인 n-butanol과 n-butyric acid를 다량 생산하는 미생물은 Clostridium tyrobutyricum, Clostridium beijerinckii, Clostridium acetobutylicum이다. 대표적인 C6 물질인 n-hexanoic acid는 Clostridium kluyveri와 Megasphaera elsdenii가 다량 생산한다. 미생물 발효에 의해 보고된 n-butanol, n-butyric acid, n-hexanoic acid의 최대 생산량은 각각 21, 55, 19 g/L이었다. 배양과정에서 이들 생산물의 제거는 최종산물억제의 감소로 미생물에 의한 n-butanol, n-butyric acid, n-hexanoic acid의 생산량을 증가시켰다. 특히 C6 물질인 n-hexanoic acid는 n-hexanol로 될 수 있는 고 부가가치 물질로 생물학적 생산 연구가 꾸준히 진행 중인데, 신규한 미생물인 Clostridium sp. BS1은 galactitol을 이용하여 5 g/L의 n-hexanoic acid를 생산하였다. Depletion of petroleum increased the need of alternative energy and chemical resources. Biomass, a renewable resource, can be transformed to bioenergy and biomaterials, and the materials from biomass will ultimately substitute petroleum based energy and chemical compounds. In this perspective, production of C4-C6 compounds for bioenergy and biomaterials are described for understating of current research progress. n-Butanol and n-butyric acid, the major C4 compounds, are produced by Clostridium tyrobutyricum, Clostridium beijerinckii, and Clostridium acetobutylicum. n-Hexanoic acid, a typical C6 compound, is produced by Clostridium kluyveri and Megasphaera elsdenii. Reported maximum amount of n-butanol, n-butyric acid and n-hexanoic acid was 21, 55, and 19 g/L, respectively, and extraction of these C4-C6 compounds are induced increase production by those anaerobic bacteria. In addition, a new bacterium Clostridium sp. BS-1 produced 5 g/L of n-hexanoic acid using galactitol.
생물학적 수소생산을 위한 Trickling Bed Biofilter에서의 친수성과 소수성 담체의 영향
전병승(Jeon, Byung-Seung),이선미(Lee, Sun-Mi),김용환(Kim, Yong-Hwan),채희정(Chae, Hee-Jeong),상병인(Sang, Byoung-In) 한국신재생에너지학회 2006 한국신재생에너지학회 학술대회논문집 Vol.2006 No.06
Two mesophilic trickling bed bioreactors filled with two different types of media, hydrophilic- and hydrophobic-cubes, were designed and tested for hydrogen production via anaerobic fermentation of sucrose. Each reactor consisted of a column packed with polymeric cubes and inoculated with heat-treated sludge obtained from anaerobic digestion tank. A defined medium containing sucrose was fed with changing flow rate into the capped reactor, hydraulic retention time and recycle rate. Hydrogen concentrations in gas-phase were constant, averaging 40% for all conditions tested. Hydrogen production rates increased up to 10.5 L{cdot};h^{-1}{cdot}L^{-1} of reactor when influent sucrose concentrations and recycle rates were varied. Hydrophobic media provided higher value of hydrogen production rate than hydrophilic media at the same operation conditions. No methane was detected when the reactor was under a normal operation. The major fermentation by-products in the liquid effluent of the both trickling biofilters were acetate and butyrate. The reactor filled with hydrophilic media became clogged with biomass and bio gas, requiring manual cleaning of the system, while no clogging occurred in the reactor with hydrophobic media. In order to make long-term operation of the reactor filled with hydrophilic media feasible, biofilm accumulation inside the media in the reactor with hydrophilic media and biogas produced from the reactor will need to be controlled through some process such as periodical backwashing or gas-purging. These tests using trickling bed biofilter with hydrophobic media demonstrate the feasibility of the process to produce hydrogen gas in a trickle-bed type of reactor. A likely application of this reactor technology could be hydrogen gas recovery from pre-treatment of high carbohydrate-containing wastewaters.
맥주박 첨가 섬유질 배합사료가 거세흑염소의 생산성 및 영양소 이용율에 미치는 영향
최순호(Sun Ho Choi),황보순(Soon Hwangbo),김상우(Sang Woo Kim),상병돈(Byung Don Sang),김영근(Young Keun Kim),조익환(Ik Hwan Jo) 한국초지조사료학회 2006 한국초지조사료학회지 Vol.26 No.4
본 연구는 맥주박을 첨가한 섬유질 배합사료가 육성기 거세흑염소의 생산성과 영양소 이용율에 미치는 영향을 조사하여 맥주박의 사료가치를 평가하고자 실시하였다. 공시축은 사양시험은 거세흑염소 40두를 4처리구(관행사료구, 맥주박 20, 30 및 40% 첨가구)로 나누어 처리구당 10두씩, 소화율 시험에서는 처리구당 3두씩 완전임의 배치하여 시험을 실시하였다. 그 결과는 다음과 같다. 맥주박 첨가 수준별 섬유질 배합사료의 일반 조성분은 건물 함량이 62.35~66.02%, 조단백질 함량은 14.49~15.36%의 범위로 나타났으며, ADF와 NDF 함량은 28.24~29.08 및 53.27~54.85%로 나타나 맥주박 첨가 비율에 따른 섬유소 함량의 차이는 없었으나, NFC 함량은 맥주박 첨가 비율이 높을수록 NFC 함량이 낮아졌다. 일당증체량은 대조구가 맥주박 첨가구보다 유의하게 높았다(p<0.05). 건물섭취량, 유기물섭취량, NFC 섭취량은 맥주박 20% 첨가구가 가장 높았고(p<0.05), 조단백질과 가소화 조단백질 섭취량은 맥주박 첨가구가 대조구 보다 높은 경향이었다. 건물, 유기물, NDF 소화율은 대조구가 맥주박 첨가구 보다 유의하게 높았으며(p<0.05), 조단백질 소화율은 맥주박 첨가구가 대조구 보다 높은 경향이었고, 질소 축적율은 대조구 보다 맥주박 첨가구가 높은 경향이었다. This study was conducted to investigate the effects of Total Mixed Ration (TMR) with wet brewer’s grain on feed intake, nutrient digestibility and nitrogen retention of castrated Korean black goat. For feeding trial, forty castrated Korean black goats were divided into four treatment groups, which were fed TMR containing 20, 30, 40% of wet brewer’s grain. Fo r digestibility trial, twelve castrated Korean black goats were allotted to treatments in four groups of three goats. Results are summarized as follows. Dry matter (DM) contents of TMR with wet brewer’s grain was 63.35~66.02%, that of crude protein was 14.49~15.36%, Acid detergent fiber (ADF) and Neutral detergent fiber (NDF) were 28.24~29.08, 53.27~54.85%, each. These were not different by supplemental level of brewer’s grain in TMR. However, Non-fibrous carbohydrate (NFC) contents were lower according to increase wet brewer’s grain in TMR. Average daily gain of control group were higher than those from TMR with wet brewer’s grain (p<0.05). Intake of DM, Organic matter (OM) and NFC of TMR supplemented with 20% of brewer’s grain were higher than those of other treatments (p<0.05). Crude protein (CP) and digestible CP intake of TMR diets supplemented wet brewer’s grain was intended to highe r than those of a control group. Digestibilities of DM, OM and NDF in control group were higher than those from TMR with wet brewer’s grain (p<0.05). Digestibilities of CP of TMR with supplemented wet brewer’s grain was tend to be higher than those of a control group. Nitrogen retention of TMR supplemented wet brewer’s grain was tend to be somewhat higher compared to the control group.
섬유질배합사료 내 조단백질 수준이 임신초기 흑염소의 건물섭취량, 소화율 및 질소출납에 미치는 영향
황보순(Soon Hwangbo),최순호(Sun Ho Choi),이성훈(Sung Hoon Lee),김상우(Sang Woo Kim),김영근(Young Keun Kim),상병돈(Byung Don Sang),조익환(Ik Hwan Jo) 韓國草地學會 2007 한국초지조사료학회지 Vol.27 No.2
본 연구는 섬유질배합사료의 조단백질 수준이 임신초기 흑염소의 사료섭취량, 영양소 소화율 및 질소 축적율에 미치는 영향을 조사하여 임신흑염소의 적정 조단백질 수준을 구명하기 위하여 실시하였다. 공시축은 임신초기흑염소 12두를 4처리구(관행사료구, 조단백질 10, 12 및 15%)로 나누어 처리구당 3두씩 대사케이지에 완전임의 배치법으로 배치하여 시험을 실시하였으며 그 결과는 다음과 같다. 섬유질배합사료의 일반 조성분은 건물 함량이 89.0~91.0%를 나타내었고, ADF와 NDF 함량은 18.87~19.85 및 53.41~54.80%로 나타나 조단백질 수준에 따른 섬유소 함량의 차이는 없었다. 조단백질 함량은 TMR10, TMR12 및 TMR14구가 각각 10.61, 12.15 및 14.97% 이었다. 하지만, NFC 함량은 섬유질배합사료내 단백질수준이 증가함에 따라 감소하였다. 건물섭취량, 영양소 섭취량 및 가소화 영양소 섭취량은 TMR15와 대조구가 TMR10과 TMR12구 보다 유의하게 높았으며(p<0.05), 대사체중당 건물섭취량과 체중에 대한 건물섭취비율에서도 대조구와 TMR15구가 유의하게 높았다(p<0.05). 건물소화율은 69.01~70.33% 범위로 나타났다. 에테르 추출물 소화율은 세 수준의 단백질을 함유하는 섬유질배합사료군이 대조군 보다 유의하게 높았으나(p<0.05), 섬유질배합사료군간에는 유의한 차이가 나타나지 않았다. 질소축적량 및 축적율에서는 TMR15구와 대조구가 다른 처리구 보다 유의하게 높게 나타났다(p<0.05). 이상의 결과에서 임신초기 흑염소사료 내 증가하는 수준의 조단백질 함량은 건물섭취량 및 질소 축적을 증가시켰고, 특히 TMR 사양에서 임신초기 흑염소의 조단백질수준은 적어도 15% 이상은 되어야 할 것으로 추정된다. This study was conducted to determine the effects of different levels (10, 12 and 15%) of crude protein (CP) in total mixed ration (TMR) on dry matter intake, digestibility and nitrogen balance of Korean black goats in the stage of early pregnancy and to obtain information on their optimal dietary levels of CP. In the present study, 12 Does of Korean black goats in the early pregnancy were allotted to four unreplicated groups by dietary level of CP and then they were housed in individual metabolism cages with completely randomized design throughout 30 days with 20 days adaptation and 10 days collection periods. Does in Control were fed a conventional diet and does in TMR10, TMR12 and TMR15 were fed a diet adjusted to about 10, 12 and 15% CP, respectively. Dry matter(DM) contents ranged from 89 to 91% in treatments. There were no differences for fiber contents among three CP levels of TMR, showing that ADF and NDF had 18.87 to 19.85, and 53.41 to 54.80, respectively. Crude protein contents for three TMR treatments had 10.61, 12.15 and 14.97%, respectively. However, non-fibrous carbohydrate (NFC) contents decreased with increasing CP levels in treatments. Meanwhile, Intakes of DM, nutrients and digestible nutrients were significantly (p<0.05) higher in TMR15 and control than in TMR10 and TMR12. Moreover, DM intake per metabolic body weight and their ratio per body weight was significantly (p<0.05) higher for control and TMR15 than other treatments. DM digestibility was not significantly different among treatments, but ether extract digestibility of treatments was significantly (p<0.05) higher than that of control, but there was no significant difference among treatments. Nitrogen retention significantly (p<0.05) increased with increasing CP levels in TMR, and TMR15 was highest among treatments. Our results showed that the increasing CP levels in TMR increased DM intake and nitrogen retention and suggested that the optimal dietary CP levels under TMR feeding system in early pregnant Korean black goats could be estimated for at least 15%.
맥주박을 첨가한 섬유질 배합사료가 번식흑염소의 영양소 이용율에 미치는 영향
최순호(Sun Ho Choi),황보순(Soon Hwangbo),김상우(Sang Woo Kim),상병돈(Byung Don Sang),김영근(Young Keun Kim),조익환(Ik Hwan Jo) 한국초지조사료학회 2006 한국초지조사료학회지 Vol.26 No.3
This study was conducted to investigate the effects of Total Mixed Ration(TMR) with wet brewer’s grain on feed intake, nutrient digestibility and nitrogen retention of breeding Korean native goat. Twelve breeding Korean native goats were divided into four treatment groups, which were fed TMR containing 20, 30, 40% of wet brewer’s grain and a control group fed concentrate feed and hay, respectively. Results are summarized as follows. Dry matter contents of TMR with wet brewer’s grain was 61.46-65.81%, that of crude protein was 14.42-15.59%, ADF and NDF were 28.32-28.52, 53.46-54.16%, each. These were not different by supplemental level of brewer’s grain in TMR. However, NFC contents were lower according to increase wet brewer’s grain in TMR. Dry matter intake of TMR supplemented with 20% of brewer’s grain was tend to be higher than those of control group. CP and digestible CP intake of TMR diets supplemented wet brewer’s grain was intended to high er than those of a control group. NFC and digestible NFC intake were significantly higher in control group than in WBG40 treatment(p〈0.05). Digestibilities of DM, ADF, NDF and NFC in control group were higher than those from TMR with wet brewer’s grain(p〈0.05). Digestibilities of CP of TMR with supplemented wet brewer’s grain was tend to be higher than those of a control group. Nitrogen retention of TMR supplemented wet brewer’s grain was tend to be somewhat higher compared to the control group.