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인체분변으로부터 분리한 유산균 Lactobacillus pentosus Miny-148의 생균제 특성 연구
정민영,박용하,김현수,부하령,장영효,Jung, Min-Young,Park, Yong-Ha,Kim, Hyun-Soo,Poo, Ha-Ryoung,Chang, Young-Hyo 한국미생물학회 2009 미생물학회지 Vol.45 No.2
우수한 생균제를 개발하기 위하여 안전성이 알려진 유산균을 대상으로 인체의 분변으로부터 300여 균주를 분리하고 내산성, 내담즙성, 내열성, 항균력, 항암 및 항바이러스 효과를 가지는 균주들을 선발하여 생균제 특성을 나타내는지를 알아보기 위해 본 실험을 수행하였다. 인체에서 분리한 여러 균주 중 Miny-148 균주는 낮은 pH 및 높은 담즙산에 대한 내성, 열처리에 대한 열안정성을 지녀 기초적인 probiotic 특성을 가진 균주로 선발되어, Lactobacillus pentosus (99.9% 상동성)로 동정되었다. 항균력 실험에서 Escherichia coli O157:H7을 비롯한 Shigella flexneri, Bacillus anthracis, Staphylococcus aureus, E. coli, Vibrio cholerae, V. vulnificus, Salmonella typhimurium, 그리고 및 methicillin-resistant S. aureus (MRSA) 균주 8종 등 총 16종의 병원성세균을 억제하였다. 또한 Miny-148은 결장암 세포인 HT-29 cell을 억제하였을 뿐 아니라, transmissible gastroenteritis virus의 생육을 저해하여 세포변성 억제효과를 가진 우수한 probiotic 특성을 지닌 균주로 분석되었다. Three hundred lactic acid bacteria isolated from human feces were studied their probiotic characters to develop potential probiotics. The properties were tested on the basis of guideline for probiotic selection protocol such as tolerance for acid or bile salt, thermal stability, antimicrobial, anticancer cell, and antiviral activity. Strain Miny-148 was selected as a potential probiotic bacterium which showed resistance to low pH, bile salts and thermal stability. On the basis of fatty acid profiles and 16S rDNA sequences analysis, the strain was identified as Lactobacillus pentosus (similarity 99.9%). The strain, L. pentosus Miny-148, showed broad antimicrobial spectrum against E. coli O157:H7, Shigella flexneri, Bacillus anthracis, Staphylococcus aureus, E. coli, Vibrio cholerae, V. vulnificus, Salmonella typhimurium, and Methicillin-resistant S. aureus (MRSA). Cell-free culture supernatant of the strain also inhibited against the growth of HT-29 colon cancer cell and transmissible gastroenterits virus.
Antiviral Activity of 3,4'-Dihydroxyflavone on Influenza A Virus
Mohammed Kawser Hossain,최혜연,황재선,Ahmed Abdal Dayem,김정현,김영봉,부하령,조쌍구 한국미생물학회 2014 The journal of microbiology Vol.52 No.6
Influenza virus infection causes thousands of deaths and millionsof hospitalizations worldwide every year and the emergenceof resistance to anti-influenza drugs has promptedscientists to seek new natural antiviral materials. In this study,we screened 13 different flavonoids from various flavonoidgroups to identify the most potent antiviral flavonoid againsthuman influenza A/PR/8/34 (H1N1). The 3-hydroxyl groupflavonoids, including 3,2'-dihydroxyflavone (3,2'-DHF) and3,4 -dihydroxyflavone (3,4'-DHF), showed potent anti-influenzaactivity. They inhibited viral neuraminidase activityand viral adsorption onto cells. To confirm the anti-influenzaactivity of these flavonoids, we used an in vivo mousemodel. In mice infected with human influenza, oral administrationof 3,4'-DHF significantly decreased virus titers andpathological changes in the lung and reduced body weightloss and death. Our data suggest that 3-hydroxyl group flavonoids,particularly 3,4'-DHF, have potent antiviral activityagainst human influenza A/PR/8/34 (H1N1) in vitro and invivo. Further clinical studies are needed to investigate thetherapeutic and prophylactic potential of the 3-hydroxylgroup flavonoids in treating influenza pandemics.