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N-butyl- N-(4-hydroxybutyl)nitrosamine에 의한 흰쥐 방광암 발생 과정에서 세포주기 관련인자 단백(Cyclin D1, A, E, and B)의 발현
권귀영,박언섭,봉성근,이태진,김미경,유재형,송계용 대한병리학회 2003 Journal of Pathology and Translational Medicine Vol.37 No.4
Background : Cell cycle deregulation plays a major role in chemical multistage carcinogenesis. Therefore, the evaluation of cell cycle proteins is important. Methods : In order to induce carcinogenesis in the rat urinary bladder, 0.05% N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) was administered to male Sprague-Dawley rats for 30 weeks. Expressions of cyclin D1, A, E, and B1 were examined by immunohistochemical stainings. Results : Urothelial cell hyperplasia appeared at 5 weeks, followed by papilloma at 10 weeks. Superficial carcinoma was observed at 20 weeks, and invasive carcinoma developed in 40% (4/10) of the rats at 30 weeks. Expressions of cyclin D1 and A increased sequentially from normal mucosa through hyperplasia, papilloma, and carcinoma (p<0.01). Expressions of cyclin D1, B1 and cyclin E were higher in invasive carcinomas than in superficial carcinomas (p<0.01). In contrast, there was no significant difference in the expression of cyclin B1 between hyperplasia, papilloma and superficial carcinoma. Conclusions : The present results indicate the important roles of cyclin D1 and A in the development of BBN-induced urothelial carcinoma of rats. Aberrant expression of cyclin B1 and E may contribute to the progression from superficial to invasive bladder cancer rather than tumorigenesis.
Expression of Metallothionein in Diethylnitrosamine-induced Rat Hepatocellular Carcinogenesis
Shin, Dong Yong,Park, Eon Sub,Choi, Byung Sun,Lee, Hyn Sun,Bong, Sung Gen,Lee, Tae Jin,Yoo, Jae Hyung 중앙대학교 의과대학 의과학연구소 2002 中央醫大誌 Vol.27 No.1
The object of this work was to investigate the role of expression of metallothionein(MT)in diethylnitrosamine(DEN)-induced rat liver carcinogenesis. Futhermore, to clarify biologic significance of positive foci of MT expression, cell proliferative and apoptotic indices were examined. Heaptocellular lesions were classified into two catogories, hyperplastic lesions(more detailed in clear cell nodules, eosinophilic nodules, and basophilic nodules)and heaptocellular carcinoma. MT expression was increased in the early heaptocellular hyperplastic lesions, especially in clear cell nodules and eosinophilic nodules. However, the expression was decreased in basophilic nodules and heaptocellular carcinoma lesions. In addition, MT expressing hyperplastic lesions showed increased cell proliferative activities and some resistance to apoptosis, and lacks of MT expression may contribute to tumor progression.