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차별화서비스를 위한 Mobile IPv6에서 AAA인증절차의 성능향상을 위한 Fast Handoff 적용방안 연구
변광호 ( Kwang-ho Byun ),문영성 ( Young-song Mun ) 한국정보처리학회 2004 한국정보처리학회 학술대회논문집 Vol.11 No.1
초고속 인터넷 서비스가 일반화되고, PDA 및 노트북 등의 휴대 이동 컴퓨팅 기기의 사용이 증가하면서 인터넷 환경이 유선망에서 무선망으로 점차 변화하고 있다. 무선 인터넷 제공을 위한 주요 인프라는 이동 통신망이나 무선 랜 망이며, 서비스 가입자들은 무선망에서도 유선망과 같은 높은 품질과 안전한 서비스를 요구하고 있다. 그러나 무선망은 유선망에 비해 외부로부터의 공격에 매우 취약하므로 사용자의 QoS 요구사항 뿐만이 아니라 안전한 통신을 보장해야 한다. 현재 표준화 기관인 IETF의 Mobile IPv6 워킹그룹에서도 보안문제를 가장 중요하게 다루고 있으며, 기존의 보안 기법들의 취약성을 극복하기 위한 방안으로 표준작업 그룹에서는 인프라 차원의 AAA인증 절차를 이용한 이동노드의 인증 방안이 연구되고 있다. 본 눈문에서는 무선인터넷 가입자의 안전성과 서비스 품질을 보장하기 위해 차별화 서비스를 적용한 Mobile IPv6와 AAA연동 방안을 제안 하였으며, AAA 인증 절차에 따르는 핸드오프 지연을 줄이기 위해 Fast Handoff를 적용한 방안을 제안한다.
대장균에서 재조합 인간 Interleukin 4 의 생산 , 정제 및 면역조절활성의 측정
양영,윤석란,이충은,변광호 ( Young Yang,Suk Ran Yoon,Choong Eun Lee,Kwang Ho Pyun ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.1
The recombinant human interleukin 4 (rhIL-4) has been over expressed in E. coli transformed with expression vector pET-3b containing bacteriophage T7 promoter, into which the hIL-4 cDNA was subclond. The insolubility of the recombinant protein offered an advantage of purification in only a few steps. The recombinant human IL-4 was refolded using reduced/oxidized glutathione to restore the proper conformation and purified to homogeneity by one passage over ion exchange column. The purified protein was shown as a single band on SDS-PAGE. The refolded rhIL-4 was characterized by nucleotide sequence analysis and bioassays. The purified rhIL-4 has biological activities on B cell proliferation and induction of B cell differentiation antigen, CD23, which strongly indicates that the protein is folded correctly.
대장균에서 재조합 인간 Interleukin 6의 대량생산 및 그 생물학적 활성의 측정
양영,강형식,나우진,이충은,변광호 ( Young Yang,Hyung Sik Kang,Woo Jin Na,Choong Eun Lee,Kwang Ho Pyun ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.2
Human interleukin 6 (hIL-6) is a multifunctional cytokine involved in acute phase and immune response. While a critical role of IL-6 in various immune disorders has been suggested, studies on its detailed functional mechanism are often hampered due to the low natural abundance of this cytokine. Thus, for a large scale production of hIL-6, we have made an attempt to directly express hIL-6 using pET-8c expression plasmid under the control of T7 promoter in Escherichia coli. A cDNA coding for hIL-6 without the signal sequence was amplified using PCR reaction, fused to pET-8c, and transformed into E. coli (λDE3). Upon induction with isopropyl thiogalactoside, recombinant human interleukin 6 (rhIL-6) was overexpressed as a form of inculsion bodies, with its expression level reaching over 30% of total E. coli proteins. The rhIL-6 was isolated from inclusion bodies by solubilization in 6 M guanidine hydrochloride followed by dialysis against 50 mM Tris buffer. A single passage over DEAE-Sepharose faciliated the purification of rhIL-6. The purified rhIL-6 was characterized by nucleotide sequence analysis and bioassays. The biological activity was confirmed by proliferation of B9 cells and immunoglobulin secretion of SAC-blast B cells.
김성숙(Sung Sook Kim),김도영(Doe Young Kim),문일환(Il Whan Moon),변광호(Kwang Ho Pyun),최인표(In Pyo Choi) 대한소화기학회 1995 대한소화기학회지 Vol.27 No.4
N/A Rackground/Aims: Interleukin-6 (lL-6), also known as B cell stimulatory factor 2(BSF-2), induces the final maturation of B cells to antitxxiy-producing cells. IL-6 has many biologic properties including the immune and intlammatory responses. This study wos aimed to evaluate the role of local interleukin 6(IL-6) in the pathogenesis of chronic hepatitis. Methods; We examined the cellular site and grade of IL-6 staining in paraffin sections of the liver from 24 patients with liver disease, using immunohistochemistry with a polyclonal antitwdy. The patient. Were divided into two groups; Group A(n=l3) with high histologic uctivi1y consisted of CAH-type B(n=10) ond active cirrhosis(n=3), whilc Group B(n= l l) with low hi.itologic activity consisted of CPH-type B(n=4), inactive cirrhosis(n=2) and fatty liver(n=S). Results: There was no staining of IL-6 in normal liver tissue. Thv grade.I of IL-6 staining in Group A were three positive in seven cases (53.81o), two positive in five ca.ics(38.3%) and one positive in only one case(7.7%), while those in Group B were one positivc in three cases(27.3%) ancl trace in eight case.(72.7ln). IL-6 stained cells in chronic hepatitis were hepatocytcs, cspecially in the areu ot' piecemeol necrosi.I, bilc duct cel1., infiltrating inflammatory cells and endothelial cell.I. The score of histological activity index(HAJ), piecemeal necrosis and fibrasis and thc gradv. Of 1L-6 staining of Group A were ull significantly higher than those of Group B. The grade of IL-6 staining and HAI werc well correlated(r =0.74, p 0.0l), Conclusion: Locally produced IL-6 in the liver may contribute to the inflammatory process and immunological response in chronic hepatiti.. (Korean 3 Gastroenterol 1995;27:403-411)