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백생글,박정호,나채선,이별하나,정효정,우수영 한국산림과학회 2018 Forest Science And Technology Vol.14 No.3
The present study was conducted to examine the response of Pterocarpus indicus to elevated ozone by comparing the effects between well-watered and soil drought treatments. The ozone concentration was 200 ppb and the exposure was conducted for a month with wellwatered and soil drought treatments in chamber environmental conditions. Elevated ozoneinduced visible injuries to P. indicus varied among the well-watered environmental conditions, with the appearance of light green chlorosis in the SWO seedlings over time. However, in the SDO seedlings, no difference was visible between drought and drought with ozone treatment. Shriveled leaves were evident because of the water deficiency. Moreover, elevated ozone induced leaf stomata injury and a harsh abaxial surface, whereas drought stress induced less injured stomata because of stomata closing. In addition, the palisade tissues exhibited smaller chloroplasts, damaged thylakoid, increased plastoglobuli, decreased starch grain and thinner cell walls on the upper leaf surface. Thus, the SDO seedlings were less influenced by elevated ozone stress than SWO seedlings due to the reduced ozone absorption which caused by stomata closing to prevent water loss.
김기우,백생글,박병준,김현욱,류임주,Kim, Ki-Woo,Baek, Saeng-Geul,Park, Byung-Joon,Kim, Hyun-Wook,Rhyu, Im-Joo 한국현미경학회 2010 Applied microscopy Vol.40 No.4
집속이온빔장치(focused ion beam, FIB)는 전자보다 무거운 양이온빔을 이용하여 시료를 10~100 nm 정도로 깎아 낼 수 있는 장비로 주로 재료분야에서 활용되어 왔다. 최근 세계적으로 의생물 분야에서의 활용이 점차 늘어나고 있는 추세에 있어 국내연구자들의 이해를 돕기 위해 간단히 기기의 메커니즘과 그 활용예를 기술 하고자 한다. FIB에 주로 사용되는 갈륨(Ga)빔의 특성 때문에 시료의 표면을 효과적으로 쳐 낼 수 있고, 전계 방사형 주사전자현미경(FESEM)을 이용하면 그 표면의 영상을 얻을 수 있다. 이 두 가지 시스템을 하나의 시스템으로 묶어낸 것을 dual beam system이라고 한다. 최근 이러한 시스템을 이용하여 효모, 병원균에 감염된 식물 등이 소개되었으며, 통상적으로 경도가 높아 처리하기 힘든 상아나 어패류의 껍질 등의 시료를 효과적으로 분석한 연구도 있다. 또한 FIB를 이용한 밀링과 FESEM을 이용한 절단면 촬영을 반복하여 얻는 영상을 이용하여 신경계의 연결망을 재구성하려는 연구가 활발하게 진행되고 있다. FIB/FESEM dual beam은 의생물학 분야의 다양한 연구에 활용될 수 있는 유용한 도구며, 의생물 시료의 3차원 연구에 크게 기여할 수 있을 것으로 판단된다. A focused ion beam (FIB) system produces a beam of positive ions (usually gallium) which are heavier than electrons and can be focused by electrostatic lenses into a spot on the specimen. With its ability milling of the specimen material by 10 to 100 nm with each pass of the beam, FIB is widely adopted in materials science, semiconductor industry, and ceramics research. Recently, FIB has been increasingly employed in the field of biomedical sciences. Here we provide a brief introduction to FIB and its applications for a wide variety of biomedical research. The surface of specimen can be in situ processed and quasi-real time visualized by two beam combination of FIB and field emission scanning electron microscope (FESEM). Due to its milling process, internal structures can be exposed and analyzed: yeast cells, fungus-inoculated wheat leaf, mannitol particles in inhalation aerosols, and oyster shell. Serial blockface tomography with the system kindles 3-dimensional reconstruction researches in the realm of nervous system and life sciences. Two-beam system of FIB/FESEM is a versatile tool to be utilized in the biomedical sciences, especially in 3-dimensional reconstruction studies.
Volumetric equation development and carbon storage estimation of urban forest in Daejeon, Korea
박정호,백생글,권민영,제선미,우수영 한국산림과학회 2018 Forest Science And Technology Vol.14 No.2
This study was performed to develop equations which are suitable for estimating carbon storage of urban forest in Daejeon, Korea and to evaluate carbon storage and sequestration of urban forest using developed equations. Among the urban forest, we only concern about the major street tree species (Platanus occidentalis, Ginko biloba, Zelkova serrata, Chionanthus retusa, and Acer pseudosieboldianum) which are planted alongside a road for vehicles as well as pedestrian in urban area. Developed equations showed R2 of 0.93 (Z. serrata), 0.67 (P. occidentalis), 0.96 (G. biloba), 0.93 (C. retusa), and 0.88 (A. pseudo-sieboldianum) when they were derived from diameter at breath height (DBH). Using the DBH-volume equation, the five street tree species (P. occidentalis, G. biloba, Z. serrata, C. retusa, and A. pseudo-sieboldianum), which account for 70% of total street trees in the Daejeon, stored about 4290 tons of carbon. Among the five street tree species, P. occidentalis constituted more than 50% of total stored carbon and followed by G. biloba. Based on the carbon storage, five street tree species can sequestrate 216 tons of carbon annually which means that 793 tons of carbon dioxide (CO2) removal from the air every year.
신정원,지용훈,김정민,이지원,채정현,양초현,백다슬,백생글,윤현원,이하나,서정민,김영률,남재익,안창호 인간식물환경학회 2022 인간식물환경학회지 Vol.25 No.6
Background and objective: Kirengeshoma koreana Nakai is an endemic and endangered species in South Korea. We conducted in vitro propagation and regeneration of K. koreana from stem cuttings to investigate the effects of nine different basal culture media and five different carbohydrate sources on its growth. Methods: Apical segments (at least 1 cm long) collected randomly from a six-week-old K. koreana plantlet grown in vitro were used as explants. In the first experiment, the explants were transferred into square vessels containing 50 mL of nine different basal culture media supplemented with 30 g⋅L-1 sucrose and 3 g⋅L-1 Phytagel. In the second experiment, the explants were transferred into square vessels containing 50 mL half-strength SH medium supplemented with five different carbohydrate sources at 30 g⋅L-1. Each medium was solidified with 3 g⋅L-1 Phytagel. All experiments contained 4 cultures, and the experiments were repeated four times to enhance reproducibility. Data on stem length, shoot fresh weight, leaf width, leaf length, root count, and root length were collected at the end of 8 weeks of culture. Results: ANOVA showed that the basal culture medium had a significant effect on K. koreana growth (p < .001). The half-strength SH medium was the best condition for stem length, shoot fresh weight, leaf width and root length (3.76 ± 0.12 cm, 0.60 ± 0.06 g, 1.19 ± 0.05 cm, and 2.83 ± 0.13 cm, respectively). However, the highest percentage increase in root count (13.00 ± 0.90) was found to occur with half-strength WPM. The effect of different carbohydrate sources on K. koreana growth was significantly different (p < .001), with the exception of stem length and leaf width (p = .26 and p = .09, respectively). Maltose was the best condition for shoot fresh weight (0.90 ± 0.09 g). Although there was no significant difference, sucrose was found to be best for leaf width, leaf length and root length (1.34 ± 0.07 cm, 2.34 ± 0.10 cm, and 3.86 ± 0.19 cm, respectively). Conclusion: This in vitro propagation and regeneration system for K. koreana shows promise in terms of scalability and could help greatly with germplasm conservation and restoration efforts for the species.