27. 한국인 신상남아에서 관찰된 VATER 증후군 1례

류임주,신영철 대한체질인류학회 1990 대한체질인류학회 학술대회 연제 초록 Vol.33 No.-

전자게시판을 이용한 문헌상호대차시스템의 제안

류임주,김덕훈 한국의학도서관협의회 1990 한국의학도서관협의회지 Vol.17 No.1-2

전자게시판을 이용한 문헌상호대차시스템의 제안

류임주,김덕훈 한국의학도서관협회 1990 한국의학도서관 Vol.17 No.1-2

흰쥐 부신수질 아민성세포의 분비과정에 관한 전자현미경적 관찰

류임주,엄창섭,서영석,Rhyu, Im-Joo,Uhm, Chang-Sub,Suh, Young-Suk 한국현미경학회 1992 Applied microscopy Vol.22 No.1

To clarify the exocytotic features in adrenal medullary aminergic cells, the authors observed rat adrenal medulla prepared by the TAGO method with transmission electron microscope. Rat adrenal medulla contains two types of aminergic cells, adrenergic and noradrenergic, as described. They were present as a group. In a single group both adrenergic and noradrenergic cells were present, but the same kind of cells showed the tendency forming small groups. Adrenergic cells were characterized with the granules having relatively electroluscent cores. These granules were relatively uniform in size, and the cores filled the granules with only thin halos. Noradrenergic cells were characterized with the granules of various size and forms. Most of the cores of these granules were generally more electron-dense than those of the adrenergic cells and only partly filled the granules without forming the halos. But, some granules were very similar in the shape and electron density as those of the adrenergic cells. Even empty-looking granules were present. Exocytotic figures with the classical omega figures were observed in both types of aminergic cells, but they were more frequent in adrenergic cells. These figures were mainly present along the plasma membranes toward the capillary. The excreted materials could be identified in the cleft of the omega figures. Apocrine-like secretory patterns but without cytoplasmic rims were identified in noradrenergic cells. Some vesicles, possibly formed from the cytoplsmic tubular systems were released. Some irregular lamellar structures of varying sizes were also observed. They looked like membranous structures sneaking through the plasma membranes. We could not, however, found any evidences of their involvement in exocytotic processes. These were present toward the capillaries and found only in the adrenergic cells. The authors conclude that the secretory processes in adrenal chromaffin cells may include not only the classical exocytotic processes but also the unusual direct secretions of granules or parts of cellular organelles. The membranous lamellar structures may indicate the remnants of excreted granules or functionally inactive excess membranes of the organelles removed from the cytoplasm.

흰쥐뇌에서 경련발작에 의한 heat shock protein 70 유전자 발현양상

김현,류임주,정혜선,서영석 대한체질인류학회 1995 대한체질인류학회 학술대회 연제 초록 Vol.38 No.-

자궁근종에서 Phospholipase C 동종효소들의 발현에 관한 연구

윤혜원,류임주,김창미,심재철,서영석 대한해부학회 1995 Anatomy & Cell Biology Vol.28 No.4

고양이 심장에서 관상동맥 결찰과 재관류로 인한 ATPase 변화에 관한 세포화학적 연구

김은기,류임주,엄창섭,서영석 고려대학교 의과대학 1991 고려대 의대 잡지 Vol.28 No.1

To understand the behaviors of Ca^(++)-ATPase and Na^(+)-K^(+) ATPase in ischemic and ischemic-reperfused heart, and to elucidate their roles in those conditions, the authors observed cat myocardium ultracytochemically. Acute ischemia was induced by ligation of the anterior interventricular branch of left coronary artery. Reperfusion was begun after 20minutes of ischemia and discontinued after 1-2 minutes when ventricular fibrillation appeared on EKG. The reaction products of Ca^(++)-ATPase were irregular In shape, and distributed mainly in myocytes in the vicinity of capillaries. In normal cardiac myocytes, they were present at plasmalemma, sarcoplasmic reticulum, transverse tubules, intercalated discs including intermediate junctions, desmosomes and gap junctions. Some were scattered along with inner mitochondrial membranes and myofibrils. After 20 minutes of ischemia. almost all structures had negligible reaction products. except some dilated sarcoplasmic reticulum beneath the plasmalemma, which retained strong reactivities. After reperfusion, the reaction products showed a tendency to be Increased. But there was hardly any reaction products in mitochondria. Patches of myofibrils with much increased enzyme activity than other myofibrils were found scattered. In addition, macrophages showed strong reactivity throughout their nuclei. Endothelial cell nuclei, however, were free of reactions in all conditions. The reaction products of Na^(+)-K^(+) ATPase were fine In nature. In normal cardiac myocytes, they were present at plasmalemma, transverse tubules, sarcoplasmic reticulum, various structures of intercalated discs, myofibrils, mitochondria, inner nuclear membranes and heterochrormatin. In ischemic heart, the reactions decreased generally and increased after reperfusion. Mitochondria in normal states had many fine reaction products scattered throughout them to the degree of masking their details. In ischemic conditions, the reaction products, decreased in number, were located contiguous to inner mitochondrial membrane. After reperfusion each reaction product was increased in size, although the amount was less than normal conditions. Gap junctions were unique structures'in that they lacked reactivity both in ischemic and ischemic-reperfused conditions. Other structures of the intercalated discs, especially desmesomes, showed increased reactions. Some myofibrils showed much strong reactions in ischemic and ischemic-reperfused groups, even though the reactivity at myofibrils as a whole was decreased. Under all conditions, the nuclei of interstitial cails and endothelial cells had their reaction products related to the nuclear membranes and heterochrormatin. The authors, based on the above observations, conclude that Ca^(++)-ATPase may have little role in ischemic and ischemic-reperfused conditions. Na^(+)-K^(+) ATPase during reperfusion, however, even depressed in their activities, may try to normalize the decreased intramitochondrial ATP contents and the increased intracellular Na^(+) concentrations resulted from ischemic conditions.

근신경 재생시 근방추와 전기관 변화에 관한 조직 계측학적 연구

강학정,서영석,류임주 고려대학교 의과대학 1991 고려대 의대 잡지 Vol.28 No.1

To understand the regenerative changes of muscle spindles and tendon organs after nerve injury of skeletal muscles, the distribution, shape, size and structure of muscle spindles and tendon organs were observed light microscopically at the reinnervated rat soleus muscles of the pretreated as followings : Group Ⅰ: crushing Injury of tibial nerve, Group Ⅱ: simple suture of cut tibial nerve, Group Ⅲ : exchanged suture of severed tibial and fibular nerves. Soleus muscles and nerves were inspected with light microscopy and electron microscopy for their quality of repairment. 1. The number of myelinated axon of control group were 161, the treated group were 128,145 and 117 respectively in group Ⅰ, Ⅱ, and Ⅲ. The group Ⅰ showed good recovery compared with cut and sutured groups from the view point of diameter of axons, thickness of myelin sheath and their distribution, although the number of myelinated and unmyelinated fibers of Group Ⅱ were most abundant. 2. The muscles of group Ⅰ marked much better recovery than the other groups by 90% of cross sectional area of control group. 3. The number of muscle spindles recovered more than 80% of control group and there were no significant differences among three experimental groups. 4. The lengths of muscle spindles did not show meaningful changes in almost cases except group Ⅲ with slightly long ones. 5. The tandem linkages of spindles were 1 in control and group Ⅰ, 3 in group Ⅱ and Ⅲ. The spindles with underdeveloped in their equatorial swellings were observed 2 in control, 4 in group Ⅰ and 6 in group Ⅱ and Ⅲ which showed the branched type muscle spindles and the muscle spindle continued with extrafusal muscle fibers. 6. The tendon organs were observed 8 in control, 3 in group Ⅰand none in the other groups. 7. No Paccinian corpuscles were found in all reinnervated muscles. Thus, the recovery state of skeletal muscles are remarkable in crushing injured group to be compared with two cut and sutured groups. The muscle spindles, however show relatively good regenerative activity in all groups without significant differences. It seems that the tendon organ and paccinian corpuscle are hardly regenerated.

[구연20]한국인 척수원뿔의 높이 연구 - MRI

김태식,박수연,류임주 대한체질인류학회 2004 대한체질인류학회 학술대회 연제 초록 Vol.47 No.-

의생물 연구 분야에서 집속이온빔장치의 응용

김기우,백생글,박병준,김현욱,류임주,Kim, Ki-Woo,Baek, Saeng-Geul,Park, Byung-Joon,Kim, Hyun-Wook,Rhyu, Im-Joo 한국현미경학회 2010 Applied microscopy Vol.40 No.4

집속이온빔장치(focused ion beam, FIB)는 전자보다 무거운 양이온빔을 이용하여 시료를 10~100 nm 정도로 깎아 낼 수 있는 장비로 주로 재료분야에서 활용되어 왔다. 최근 세계적으로 의생물 분야에서의 활용이 점차 늘어나고 있는 추세에 있어 국내연구자들의 이해를 돕기 위해 간단히 기기의 메커니즘과 그 활용예를 기술 하고자 한다. FIB에 주로 사용되는 갈륨(Ga)빔의 특성 때문에 시료의 표면을 효과적으로 쳐 낼 수 있고, 전계 방사형 주사전자현미경(FESEM)을 이용하면 그 표면의 영상을 얻을 수 있다. 이 두 가지 시스템을 하나의 시스템으로 묶어낸 것을 dual beam system이라고 한다. 최근 이러한 시스템을 이용하여 효모, 병원균에 감염된 식물 등이 소개되었으며, 통상적으로 경도가 높아 처리하기 힘든 상아나 어패류의 껍질 등의 시료를 효과적으로 분석한 연구도 있다. 또한 FIB를 이용한 밀링과 FESEM을 이용한 절단면 촬영을 반복하여 얻는 영상을 이용하여 신경계의 연결망을 재구성하려는 연구가 활발하게 진행되고 있다. FIB/FESEM dual beam은 의생물학 분야의 다양한 연구에 활용될 수 있는 유용한 도구며, 의생물 시료의 3차원 연구에 크게 기여할 수 있을 것으로 판단된다. A focused ion beam (FIB) system produces a beam of positive ions (usually gallium) which are heavier than electrons and can be focused by electrostatic lenses into a spot on the specimen. With its ability milling of the specimen material by 10 to 100 nm with each pass of the beam, FIB is widely adopted in materials science, semiconductor industry, and ceramics research. Recently, FIB has been increasingly employed in the field of biomedical sciences. Here we provide a brief introduction to FIB and its applications for a wide variety of biomedical research. The surface of specimen can be in situ processed and quasi-real time visualized by two beam combination of FIB and field emission scanning electron microscope (FESEM). Due to its milling process, internal structures can be exposed and analyzed: yeast cells, fungus-inoculated wheat leaf, mannitol particles in inhalation aerosols, and oyster shell. Serial blockface tomography with the system kindles 3-dimensional reconstruction researches in the realm of nervous system and life sciences. Two-beam system of FIB/FESEM is a versatile tool to be utilized in the biomedical sciences, especially in 3-dimensional reconstruction studies.