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충치균에 대한 생리활성 생약성분의 분리 및 약효평가(1) 튜립나무잎의 항균성 성분과 안전성에 대하여
배기환(Ki Whan Bae),김봉희(Bong Hee Kim),명평근(Pyung Keun Myung),변재화(Jae Hwa Byun) 대한약학회 1990 약학회지 Vol.34 No.2
The isolation and identification of an antibacterial component, from the leaves of Liriodendron tulipifera. K. Kotch against a cariogenic bacterium Streptococcus mutans OMZ 176, were carried out for developing of anticariogenic agents. The bioactive component was elucidated as beta-liriodenolide, which was isolated newly from the leaves of L. tulipifera. The minimal inhibitory concentration (MIC) of beta-liriodenolide was 100mcg/ml and the antibacterial activity was stronger than that of berberine. beta-Liriodenolide inhibited beta-lactamase activity, 50, 100 and 200mcM beta-liriodenolide did beta-lactamase activity as 0.7, 3.5 and 19.7%, respectively. The toxicity of beta-liriodenolide was not found with the method of photohemolysis.
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송규용,배기환,명창선,김문일,박용진,윤미영,김동희,Song Gyu Yong,Bae Ki Whan,Myung Chang-Seon,Jin Wen Yi,Park Yong Jin,Yun Mi Young,Kim Dong Hee 대한동의생리학회 2005 동의생리병리학회지 Vol.19 No.3
Antitumor and anti-metastatic effects of mineral powder(MP) were studied. In the present study, MP did not exhibit the any cytotoxic activity against leukemic cells such as L1210 and U937 tumor cell lines in vitro. Also, MP did not exhibit the any cytotoxic activity against solid cells such as A549 and B16-BL6 tumor cell lines in vitro. However, in vivo, MP exhibit a significant antitumor activity in BDF1 mice bearing Lewis lung carcinoma cells(LLC) with inhibition rates of 46 and $23\%$ at 200 and 100 mg/kg/day, respectively. Furthermore, in pulmonary colonization assay, MP exhibit the inhibitory effect of tumor metastasis. From these results, it was concluded that MP had antitumor and anti-metastatic activity suggesting its application for the prevention and treatment of cancer.
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홍화 추출물이 치주인대세포, 조골세포 활성도에 미치는 영향
류인철,이용무,구영,배기환,정종평,Rhyu, In-Chul,Lee, Yong-Moo,Ku, Young,Bae, Ki-Whan,Chung, Chong-Pyoung 대한치주과학회 1997 Journal of Periodontal & Implant Science Vol.27 No.4
Safflower(Carthamus tinctorius $Linn\acute{e}$ has been traditionally used for the treatment of blood stasis, and Dipsasi Radix has been used as a drug for fracture in Chinese medicine. The purpose of present study was to examine the biologic effects of safflower extract and Disasi radix extracts on the periodontal. ligament cells and osteoblastic cells and on the wound healing of rat calvarial defect. The ethanolic extract of safflower blossom, safflower seed and Dipsasi Radix(125, 250, and 500 ${\mu}g/ml$) were prepared as test group, and PDGF-BB(lOng/ml) and unsafonifiable fraction of Zea Mays L.(125, 250, and 500 ${\mu}g/ml$) were employed as positive control. The effects of each agents on the growth and survival, ALPase activity, expression of PDGF-BB receptor, chemotactic response of PDL cell and ATCC human osteosarcoma MG63 cells in vitro were examined. The tissue regenerative effect of each extracts was evaluated by histomorphometric measuring of newly formed bone on the 8mm defect in rat calvaria after oral administration of 3 different dosages groups : 0.02, 0.1 and 0.35g/kg, per day. It was also employed the same dosages of unsaponifiable fraction of Zea Mays L. as positive controls. Safflower blossom extract, safflower seed extract, and Dipsasi Radix extract stimulate the cellular activity of MG63 cells in concentration range of $125-500{\mu}g/ml$, and safflower bolssom extract and safflower seed extract stimulate also the cellular activity of periodontal ligament cells in concentration range of $250-500{\mu}g/ml$. In activity of ALPase, $250-500{\mu}g/ml$ of safflower blossom extracts showed significant stimulating effects on MG63 cells, and the same concentration range of safflower seed extracts showed significant effect on periodontal ligament cells. In the recovery on PDGF-BB receptor expression which was depressed by $IL-1{\beta}$, $125-250{\mu}g/ml$ of safflower blossom extracts and $250-500{\mu}g/ml$ of safflower seed extracts showed significant increasing effect on MG63 cells, and $500{\mu}g/ml$ of safflower blossom extract and $250-500{\mu}g/ml$ of safflower seed extracts showed significant effect on periodontal ligament cells. In chemotactic response, among all tested group, safflower seed extracts only were chemotactic to MG63 cells and periodontal ligament cells in concentration range of $125-500{\mu}g/ml$. Also in the view of bone regeneration in rat calvarial defect model, the only group that was orally administrated 0.35g/kg, day of safflower seed extract showed significant new bone formation. These results suggested that safflower extracts might have a potential possibilities as an useful drug for adjunct to treatment for regeneration of periodontal defect.
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傾斜地果樹園의 開園方法과 土壤管理法이 土壤浸蝕防止에 미치는 效果
姜炳九,裵基煥 진주산업대학교 1969 論文集 Vol.3 No.-
완 傾斜地에 있어서 開園方法과 土壤管理法이 土壤浸蝕防止에 미치는 效果를 調査하기 위한 方法으로 本實險에서는 傾斜地 果樹園에서 채용되리라 생각되는 몇가지 開園方法과 土壤管理法을 달리하는 試險區를 두어 降雨時 流去되는 流去水量과 流去土重을 調査하였다. 1. 流去水量은 開園方法間에 있어서는 계 단밭區가 가장 적고 土壤管理法間에 있어서는 敷草區와 草生區가 標準區에 比하여 적다. 敷草區와 草生區 間에는 有意差가 없었다. 2. 流去土重은 開園方法間에 있어서는 계단밭區-테라스밭區-標準區의 順位로 土壤管理法間에 있어서는 敷草區-草生區-淸耕區의 順位로 적었다. 3. 結論으로 10˚內外의 완경사지라 할지라도 傾斜밭區로 構築하고 敷草法 土壤管理를 하는 것이 土壤浸蝕防地面으로 가장 效果的인 方法이다. In order to find effects of different establishment methods and soil management system in the hillside orchard on the soil conversation. This study was designed to investigate water quantity and soil weight run off by rain of each treatment plots. As the result of this study, it was suggested that bench terraces with grass mulch system should be the most effective method on protection orchard soil from erosion, even though it is sight solope land as 10˚.
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