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RISS 인기검색어
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- 저자 : 박혜민(Hae-Min Park) (검색결과 10 건)
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박혜민,구자형,오만진,Park, Hae-Min,Gu, Ja-Hyeong,Oh, Man-Jin 충남대학교 농업과학연구소 2009 Korean Journal of Agricultural Science Vol.36 No.1
When the cattail pollen was identified by using fibrinolytic agents, we found that the fibrinolytic activity was controlled by an enzyme. Therefore, for determining the fibrinolytic activity of cattail pollen, the fibrinolytic enzyme in cattail pollen was purified by gel filtration using DEAE-cellulose, Sephadex G-150 and HPLC. Also, its purity was certified by polyacrylamide gel electrophoresis, and its physico-chemical properties, such as pH and temperature stabilities and effects of metal, inhibitors and substrates, were examined. The specific activity, purification fold, and molecular weight of the enzyme were 38U/mg, 86.4,and 75kDa, respectively. The optimum pH for the purified enzyme was at 4.0 and it was stable at pH 4.0-6.0. The optimum temperature was $55^{\circ}C$ and it was stable at $30-60^{\circ}C$. But the enzyme began to be inactivated at $70^{\circ}C$ and its activity was totally lost at temperatures above $80^{\circ}C$. As for substrate specificity, the enzyme was most effective in dissolving fibrin, followed by whole casein, ${\kappa}$-casein, ${\alpha}$-casein, ${\beta}$-casein, and BSA. With casein as the substrate, Km value was found to be 0.44mM and the enzyme showed a high affinity for casein. As for the metal ions affecting enzyme activity, $K^+$, $Na^+$, and $Mg^{2+}$ had no effect on enzyme reaction while $Zn^{2+}$ and $Fe^{2+}$ showed potent inhibitory activity. Judging from the fact that the purified enzyme was also strongly inhibited by PMSF, iodoacetic acid, and SDA, it assumed to be a serine protease.
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신고배(Pyrus pyrifolia Nakai)를 이용한 배술 제조 과정 중 성분 변화
박혜민,이가순,홍종숙,오만진,Park, Hae-Min,Lee, Ka-Soon,Hong, Jong-Sook,Oh, Man-Jin 충남대학교 농업과학연구소 2009 Korean Journal of Agricultural Science Vol.36 No.2
For increasing the consumption of pear, the pear wines were made by using eight different kinds of yeast. The chemical properties, such as pH, acidity, ethanol and sugar contents, and the consumer acceptance of various pear wines were analyzed. In analysis result of chemical properties, the value of pH was decreased and the acidity was increased until few days, and then retained during fermentation. The ethanol content was increased and the sugar content was decreased during fermentation. The pear wine brewed by B yeast (Lalnin, Saccharomyces cerevisiae) among eight different kinds of yeast had the highest content of ethanol, also this wine was represented the most consumer acceptance by the panels who consist of two age groups such as 20's to 30's and 50's to 60's. Therefore, we chose the yeast B for analyzing the effect of additives during fermentation. As the additives, the $K_2S_2O_5$ and ammonium phosphate as the disinfectant and hemicellulase and pectinase as fermenting conditioner were added into the pear wine brewed by yeast B. When additives are added, the chemical properties were the same as followed. Te values of pH was lower than when additives were not added(control). The acidity was he highest when ammonium phosphate was added, but the acidity was not changed after 4 day fermentation. Sugar contents when added $K_2S_2O_5$ 350ppm was shown the fastest deceasing, and it was orderly decreased as control, ammonium phosphate, hemicellulase, pectinase, and $K_2S_2O_5$ 175ppm. The decreasing rate of sugar content of these 6 test groups during fermentation gradually decreased without a big changes and the after 7 day fermentation, sugar contents were seldom decreased and the final sugar content was $9.5{\sim}9.8^{\circ}Brix$. In terms of change of the content of ethanol, all test groups generated 11~12.4% ethanol after 7 day fermentation. The group where ammonium phosphate was added generated 12.4% alcohol, which is the fastest speed and the group, which hemicellulase additive was added, showed generally slow alcohol generation rate. In terms of ethanol contents, the non-additive group and $K_2S_2O_5$ additive group showed 0.05%, which is very low, and hemicellulase and pectinase additive group is more than 0.5% which is rather high.
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이병찬,박혜민,심후성,김곤섭,구자형,오만진,Lee, Bung-Chan,Park, Hae-Min,Sim, Hu-Sung,Kim, Gon-Sup,Gu, Ja-Hyeong,Oh, Man-jin 충남대학교 농업과학연구소 2009 Korean Journal of Agricultural Science Vol.36 No.2
For utilizing Cattail pollen as a raw material for functional foods, the nutrients such as free sugar, free amino acid, fatty acid composition, flavonoid content, and the biological activity within Cattail pollen were measured. The results of proximate analysis within Cattail pollen included the following readings: 12.7-13.2% of moisture, 15.7-17.8% of crude protein, 1.3% of crude fat, 7.5-7.7% of free sugar, 13.7-18.6% of crude fiber, 3.4-4.9% of ash, and 49.7-55.9% of nitrogen free extracts. The composition of free amino acids consisted of 1.923% of T. orientalis, 0.907% of T. angustata, and 0.333% of T. latifolia, which were measurements that varied significantly among different species. However, all species showed considerable portions of GABA alanine, glutamic acid, and proline. Specifically, it was shown that the GABA composition, which is known for increasing immunity while simultaneously lowering blood pressure, exceeded 50%. Therefore, this result implies that Cattail pollens have potential as a powerful utilization for functional foods. The composition of the fatty acids mainly consisted of linoeic, palmitic acid, oleic acid, and linolenic acid, and didn't show many variances across different species. Also, the total contents of unsaturated fatty acid were particularly high with a measured ratio of 67.2-76.0% value. Mineral in Cattail pollen was composed of 0.354-0.492% of K, 0.0516-0.0546% of Mg, 0.045-0.0486% of Ca, and 0.0101-0.0204% of Na. Among the Cattail pollens known as anti-oxidants, flavonoid contains 0.169-0.186% of quercetin, and therefore is the largest constituent followed by rutin making up a measurement of 0.0094-0.0147%. For the purpose of the study, the Cattail pollen and its extracts were fed to SC class rats for a span of 4 weeks. Then, the DPPH radical scavenging activity was measured from the tested rats'serums and the results showed significant variances. Also, the results indicated that the cholesterol and glucose levels in the blood were decreased which in turn led to the conclusion that the cattail pollen can help hyperlipidemia and diabetic treatments.
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불응성 용혈성 빈혈을 지닌 소아 전신홍반루푸스에서의 Rituximab 치료 1례
박주환 ( Ju Hwan Park ),임재욱 ( Jae Wook Im ),전홍길 ( Hong Kil Jun ),박혜민 ( Hae Min Park ),최승원 ( Seung Won Choi ),박상규 ( Sang Kyu Park ),오지선 ( Ji Seon Oh ) 대한류마티스학회 2014 대한류마티스학회지 Vol.21 No.4
Autoimmune hemolytic anemia (AIHA) is a relatively common cause of anemia in children and adults with systemic lupus erythematosus (SLE). Although AIHA responds to steroids, in case of refractory or steroid-dependent AIHA, immunosuppressants and intravenous immunoglobulin have been used as second line agents. Rituximab, an anti-CD20 monoclonal antibody, is emerging in the treatment of SLE refractory to conventional therapy. Herein, we report a case of delayed and sustained remission of refractory hemolytic anemia in a child with SLE, post rituximab treatment. A 12-year-old female child with dizziness was referred to our department and was diagnosed with SLE combined with hemolytic anemia and renal tubular acidosis. Since frequent relapse of hemolytic anemia had occurred during the steroid tapering course, even though she had been treated with additional immunosuppressants (azathioprine, mycophenolate mofetil), the patient received 2 doses of rituximab 500 mg at 2 weeks interval at 18 months post diagnosis. After 15 months of rituximab administration, her anemia and renal tubular acidosis were fully recovered, enough to stop all medications. She remained well without recurrence for up to 3 years and 4 months after rituximab treatment.
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균일 LED조명을 위한 자유형상 2차 광학계 설계 및 공차 분석
양재석(Jae Suk Yang),박혜민(Hae Min Park),김대찬(Dae Chan Kim),오범환(Beom Hoan O),이승걸(Seung Gol Lee) 한국조명·전기설비학회 2011 한국조명·전기설비학회 학술대회논문집 Vol.2011 No.11
A freeform lens was optimally designed for achieving an uniform illumination and also its tolerance analysis was performed by considering alignement and fabrication errores. Divergent model was chosen for establishing the ray-arriving point relation for uniform illumination, and the differential equation for the lens shape was numerically solved. The illuminance distribution on the target area was calculated with LightTools™ and the uniformity was extracted from the distribution. From the tolerance analysis, it was confirmed that the alignment error could give harmful effect on the performance.
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이가순(Ka-Soon Lee),김관후(Gwan-Hou Kim),김현호(Hyun-Ho Kim),김은수(Eun-Soo Kim),박혜민(Hae-Min Park),오만진(Man-Jin Oh) 한국식품영양과학회 2009 한국식품영양과학회지 Vol.38 No.4
씀바귀뿌리를 이용하여 침출차를 제조하고자 건조온도 및 처리조건을 달리하여 생리활성을 검토하였다. 각각의 씀바귀뿌리 침출차의 침출액에 대한 생리활성을 측정한 결과, 전자공여능은 증숙처리 후 열풍건조한 것이 98%로 가장 높았고 뒤이어 천일건조>열풍건조 후 볶음처리>열풍건조의 순으로 높았으며, 침출액의 SOD유사활성은 열풍건조 후 볶음 처리한 씀바귀뿌리 추출물에서 35.61±0.67%의 활성을 보였다. ACE저해활성은 천일건조한 것이 44.60%로 가장 저해활성이 가장 높았고, 증숙 처리 후 열풍건조한 처리구가 가장 낮아 28.89%의 저해활성을 보였고, tyrosinase 저해활성은 천일건조한 것이 44.61%로 가장 높았고 열풍건조>열풍건조 후 볶음처리>증숙처리 후 열풍건조의 순으로 저해활성을 나타내었으며, 아질산염 소거능은 pH가 낮을수록 효과가 있었으며, 특히 열풍건조 후 볶음처리를 한 씀바귀뿌리 침출차의 침출액이 모든 pH 구간에서 가장 효과적인 것을 볼 수 있었다. This study was carried out to investigate physiological functionalities of tea prepared by various thermal processing methods from Ixeris dentata root in order to elevate utilization of Ixeris dentata root as food. These methods included natural drying (ND), hot-air drying (HAD, 75℃), hot-air drying after steam (HADS, steaming at 95℃ for 30 min) and roasting after hot-air drying (RHAD, roasting at 140℃ for 2~3 min). Physiological functionalities of tea prepared by ND, HAD, HADS, and RHAD were measured as hot-water extracts and each tea was extracted by 100 ㎖ water on 1 g dried Ixeris dentata root powder. Electron donating abilities were the highest in HADS treatment at 98%. SOD-like activities were the highest in RHAD at 35.61%. Both ACE and tyrosinase inhibitory activities were the highest on ND treatment at 52.34% and 44.60%, respectively. Nitrite scavenging abilities of all treatment were the highest at pH 1.2 and the highest activity among them was on RHAD treatment at 50.5%.
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