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폴리리신을 함유한 Streptomyces albulus 배양액의 김치미생물 성장억제 효과
김광섭(Kwang-sub Kim),이갑배(Garpee Lee),선흥석(Heung-suk Sun),안치민(Chi-min Ahn),박찬영(Chanyoung Park) 한국산업융합학회 1999 한국산업융합학회 논문집 Vol.2 No.1
The Streptomyces albulus broth, when the polylysine in the broth, that has powerful growth inhibiting effect far many microbes, is its maximum, had filtered off the cells, to use the broth as preservative for keeping favorable taste of Korean Kimchi. Some microorganisms in their specific growth medium, known to deteriorate the useful nutrient of the Kimchi, has grown with different amounts of the inhibiting broth, to determine the minimum growth inhibition concentration. The ε-poly-L-lysine had been identified from the IR spectroscopic analysis of the purified poly lysine of the broth from ion exchange chromatographic separation. The content of the polylysine had been determined by methyl orange decoloration effect. Though the minimum inhibition concentration, evaluated by the naked eye based on the conventional method measuring the turbid feature after 18 hours of culture, has different values each other, the observed effects confirmed that the crude broth could be used as a natural preservative for the Kimchi in extending the fair taste.<br/>
Streptomyces albulus 세정균체에 의한 polylysine의 대량생산
선흥석,김광섭,안치민,박찬영 全南大學校 觸媒硏究所 1997 觸媒硏究 論文集 Vol.19 No.-
Streptomyces albulus 세정균체를 이용하여 항세균물질인 폴리리신의 대량생산 가능성에 대하여 조사하였다. 발효기 배양에서 배양액 pH는 6.8에서 3.2로 감소하는 동안에 pH 4.0에서 4.5사이에서 폴리리신이 최대로 축적되므로 대수말기의 균체를 멸균증류수로 세척하여 마련한 세정균체를 아미노산 생산을 향상할수 있는 구연산을 2%첨가하고 pH를 최적인 4.2로 조정한 배지로 옮겨 배양하였다. 이러한 2단계공정으로 세정균체를 배양한 경우 배양액내의 폴리리신은 일반 1단계 단순 배양에 비하여 최대 6배의 축적량을 나타내었다. The feasibility of mass production of polylysine, an antibacterial substance, employing a washed mycelium of Streptomyces albulus has been investigated. Since the maximum accumulation of polylysine had appeared between 4.0 to 4.5 of pH during fermentor cultivation of which the pH of the broth has declined to 3.2 from 6.8, the strain growing in the broth on late log phase had been washed with sterilized water, then the sole mycelium had been transferred to another medium containing 2% citric acid having pH adjusted with NaOH to optimum of 4.2 to improve the production of the amino acid. The total accumulation of polylysine by washed mycelium of Streptomyces albulus from this two step procedure has exhibited 6 times more than the normal production of the simple one step fermentation.
김광섭,심승우,나현정,조인철,정흥조,박찬영 전남대학교 촉매연구소 2001 觸媒硏究 論文集 Vol.22 No.-
Fruit are normally kept in a pouch of cold storage room in winter. In this work, a mathematical model that is emulating the fruit respiration in the pouch of the storage has been established. The calculated result is compared to the actual measured gaseous composition change of pear in the packaging pouch LDPE film incorporated with silver coated ceramic powder. The presented model, that is combining polysaccharide digestion kinetics in pear, Fickian diffusion for permeation of the film and sigmoidal adsorption equilibrium between the film and gases, predicts the respiration of the pear in the packing film fairly well.