전기적 자극이 배양 두개관 골세포의 석회화에 미치는 영향에 관한 연구

박준봉,허인식,이혜자,최영철,Park, Joon-Bong,Hur, In-Sik,Lee, Hye-Ja,Choi, Young-Chul 대한치주과학회 1997 Journal of Periodontal & Implant Science Vol.27 No.4

To date, various clinical procedures have been used to restore periodontal apparatus destroyed by periodontal disease. And then, many experimental approaches have been proceeded to develop treatment methods to promote periodontal regeneration. Mechanical, chemical treatments to enhance the attachment of periodontal tissue cells as changing the physical properties of root surfaces, bone graft procedure, and treatments for guided tissue regeneration have been used for periodontal regeneration. However, recent studies have revealed that biologic factors such as growth factors promote biologic mechanism associated with periodontal regeneration. This study was done to enucleate how ELF stimulus affect the periodontal regeneration. We can have following conclusions from this experimental results. The influence of low frequency(ELF) electric stimulus (30Hz at $lO{\mu}A$) known to promote bone formation in vivo, was evaluated for its ability to affect bone cell function in vitro. After 12 hour exposure of ELF stimulus at most appropriate densities ($5{\times}10^4\;cells/cm^2$) to increase osteoblastic cells normally, rat calvarial cells were incubated for 60 hours were used in this study. We have found ELF stimulus suppress calvarial cell proliferation and the ability of protein synthesis, enhance the alkaline phosphatase activity significantly.

황금(Scutellaria Radix)의 에타놀 추출물이 백서 치조골 형성에 미치는 영향

박준봉,허익,권영혁,배기환,정종평,Park, Joon-Bong,Herr, Yeek,Kwon, Young-Hyuk,Bae, Ki-Hwan,Chung, Chong-Pyung 대한치주과학회 1997 Journal of Periodontal & Implant Science Vol.27 No.3

The purpose of this study was to evaluate the effects of ethanolic extracts of Scutellaria Radix on the alveolar bone formation in the extract socket of rat. Thirty-six Sprague-dawley rats were used in this study. Mean body weight of rat was $130{\pm}5g$. Experimental animal were administered 0.4% ,${\beta}-aminopropionitril$(Sigma, USA) with the solid commercial food for 5 days. All the maxillary 1st molar of the rats were extracted by using of the tissue forcep under the general anesthesia with Pentobarbital sodium(Tokyo Chemical Co, Japan) injection into intraperitoneal space. All the extracted rats were divided into two group, experimental group which were feeded the solid food mixed ethanolic extracts of Scutellaria Radix, and control group which were feeded same food without reagent. At 1, 3, 5, 7, 9 and 14th days after tooth extraction, rats in both groups were serially sacrificed respectively. All the specimen were treated as usual method and prepared Hematoxylin-eosin stain for the light microscopic observation. The results were as follows : 1. Bone formation of extracted socket starts from the area on remained periodontal ligament than other area. 2. In the case of administration of the extracted Scutellaria Radix showed rapid healing process of connective tissue than non-administrated group. 3. In the case of administration of the extracted Scutellaria Radixshowed rapid osteogenesis than non-administrated With above results, it was concluded that ethanolic extracts of Scutellaria Radix may play a favorable role on the healing process of exatraction socket after extraction in rats. It was suggested that further study to evaluate the different concentration and administration method of ethanolic extracts of the Scutellaria Radix into same experimental model.

Bioceramic제제의 성견 치조골결손부의 재생에 관한 실험적 연구

박준봉,이만섭,Park., Joon-Bong,Lee, Man-Sup 대한치주과학회 1985 Journal of Periodontal & Implant Science Vol.15 No.1

구강솔의 발달과 구강위생관리의 고찰

박준봉(Joon Bong Park) 대한치과의사협회 2014 대한치과의사협회지 Vol.52 No.11

The purpose of this article is reviewed the historical background and development of oral care devices in the ancient civilization. Through an evolutional process of oral care devices, the prevalence of periodontal diseases and dental caries has revealed decreasing tendency. Because of the changing the role of the toothbrush, the name of brush should be alter from toothbrush to oral brush. Recent we can apply toothbrush to tongue cleaner to diminish the oral breathe odor. Selection of the toothbrush for fittable for each and every person is the one of important point to maintain the oral hygiene. including check up the oral hygiene status after toothbrush with disclosing solution. This review of literature suggest that the most important way to maintain the oral health shoul be included the selection of proper toothbrush for each person and confirmation of result of toothbrush in oral cavity after had learned tooth brush instruction from professional person.

[특집]리모델링 공사의 현황 및 전망

박준봉(Park Joon Bong) 대한건축학회 2005 建築 Vol.49 No.9

치아재식시 골형태형성단백이 치주조직 치유에 미치는 영향

김지숙,박준봉,이만섭,권영혁,Kim, Ji-Sook,Park, Joon-Bong,Lee, Man-Sup,Kwon, Young-Hyuk,Cho, Kyoo-Sung 대한치주과학회 2002 Journal of Periodontal & Implant Science Vol.32 No.3

The purpose of this study is to evaluate the influences of the bone morphogenetic protein (BMP) on the healing of periodontal ligament and alveolar bone after replantation of tooth, and to examine the possibility of its clinical application. 45 Sprague Dawley rats weighted about 100 gram were divided into 3 experimental groups by different dose of BMP. All the upper right and left 1st molar were extracted after 5 days feeding of 0.4% ${\beta}$-aminopropionitrile, and right molar were used as experimental group and left molar were used as control group. The root surface of experimental molar were treated with 25,50 and l00ng/ml of human recombinant Bone morphogenetic protein-4 (rh-BMP-4) with micropipet, and 1M Sodium hypochloride were used on control root surface. All the experimental animals were sacrificed as 1, 2, 4, 7 and 14 days after autoreplantation of upper 1st molar into their own position. The maxilla were disected included both side of 1st molar. The collected tissue were processed from demineralization to paraffin embeding as usual procedure, and the specimens were prepared with Hematoxylin-Eosin stain for the light microscopic evaluation. The results of this study were as follows : 1. There was no significant differences between control and experimental site on 1 and 2 days after replantation of tooth. In the case of 4th days, the evidence of tissue regeneration were observed on experimental site to compare the controls. New osteoid were revealed on high concentration of BMP at 7 days after replantation, and it became more obvious at 14 days, 2. The effect of the rh-BMP-4 coated on root surface was revealed slight influences for the prolifertion of cells of periodontium and tissue regeneration as dose-dependent pattern. 3. Bony ankylosis was observed between alveolar bone and root surface due to the remarkable amount of osteoid formation on the 14 days after replantation of root. In the conclusion, it was suggested that topical application of the rhBMP-4 on the root surface has influence on the periodontal ligament and alveolar bone. The application method of BMP on the root should be designed with calculation of proper concentration.

백서 치조골 천공결손부에서의 합성골 이식재의 재생효과

강윤경,박준봉,권영혁,허익,Gang, Yun-Kyung,Park, Joon_Bong,Kwon, Young-Hyuk,Herr, Yeek 대한치주과학회 2001 Journal of Periodontal & Implant Science Vol.31 No.2

The purpose of this study was to investigate effects of the natural coral(NC) and the hydroxyapatite/calcium sulfate hemihydrate(HA/CS) on an early stages of wound healing in the rat periodontal fenestration defects. In this experiment, twelve male rats(Mean : 520g in BW) aged 8 to 9 months were used. Experimental periodontal fenestration defects were surgically created with tapered fissure bur at the buccal surface of the left mandibular 1st, 2nd molars. The buccal aspects of molar roots were carefully denuded of their periodontal ligament through a bony window created in the left mandibles of rats under general anesthesia. Each experimental periodontal fenestration defect was grafted with natural coral and HA/CS, randomly. An area without bone graft was assigned for negative control group. At 10,35 days, rats were serially sacrificed via intracardiac perfusion with 2.5% glutaraldehyde and specimens were processed with Hematoxylin-Eosin stain for light microscopic evaluation. The results of this study were as follows : 1. The defect areas were filled with dense connective tissues at 10 days in control group. But in the test(NC, HA/CS)groups, the connective tissues around graft materials were formed more loosely and the response of inflammation by graft materials itself was not found. 2. The defect areas were filled with new osteoid tissues and new cementum was not formed on the cut root surface at 35 days in the control group. 3. New osteoid tissue formation was more prominent at 35 days in control than test groups. 4. The NC and HA/CS particles were encapsulated by loose connective tissues at 10 days and by dense connective tissues at 35 days, respectively. 5. In the test groups, resorption of graft particles was not found through the experimental time. From the above results, natural coral and hydroxyapatite/calcium sulfate hemihydrate may be biocompatible and osteoconductive and have a weak adverse reaction to the periodontal tissues.

표면처리 시간에 따른 임플란트 미세구조의 변화;HA와 양극산화 표면 임플란트

안상호,박준봉,권영혁,허익,정종혁,An, Sang-Ho,Park, Joon-Bong,Kwon, Young-Hyuk,Herr, Yeek,Chung, Jong-Hyuk 대한치주과학회 2005 Journal of Periodontal & Implant Science Vol.35 No.4

The present study was performed to evaluate the effect of tetracycline-HCL on the change of implant surface microstructure according to application time. Implant with pure titanium machined surface. HA-coated surface and TiUniteTM surface were utilized. Implant surface was rubbed with 50mg/ml tetracycline-HCL solution for $\frac{1}{2}min.$, 1min., $1\frac{1}{2}min.$, 2min., and $2\frac{1}{2}min.$ respectively in the test group. Then, specimens were processed for scanning electron microscopic observation. The results of this study were as follows. 1. Both test and control group showed a few shallow grooves and ridges in pure titanium machined surface implants. There were not significant differences between two groups. 2. In HA-coated surfaces, round particles were deposited irregularly. The roughness of surfaces conditioned with tetracycline-HCL was lessened and the cracks were increased relative to the application time. 3. The anodic oxidized surfaces showed the craterous structures. The surface conditioning with tetracycline-HCl didn't influence on its micro-morphology. In conclusion, the detoxification with 50mg/ml tetracycline-HCL must be applied respectively with different time according to various implant surfaces.

치근면의 구연산 탈회가 치은 섬유아세포의 부착 및 증식에 미치는 영향

이향,박준봉,Lee, Hyang,Park, Joon-Bong 대한치주과학회 1988 Journal of Periodontal & Implant Science Vol.18 No.2

천공형 티타늄막과 탈회동결건조골의 신생골 형성에 대한 영향

이윤호,박준봉,권영혁,허익,김종관,Lee, Yun-Ho,Park, Joon-Bong,Kwon, Young-Hyuk,Herr, Yeek,Kim, Chong-Kwan 대한치주과학회 2004 Journal of Periodontal & Implant Science Vol.34 No.1

This study was performed to evaluate bone formation in the calvaria of rabbit by the concept of guided bone regeneration with titanium mesh membrane and demineralized freeze-dried bone. The animal was sacrificed at 2 weeks, 4 weeks, 8 weeks, and 12 weeks after the surgery. Non-decalcified specimens were processed for histologic analysis. 1. The titanium mesh but the biocompatibility was excellent the cell-occlusiveness was feeble. 2. The cell-occlusiveness was feeble and also the soft tissue growth of the upper part of the newly-formed bone after operating was excellent in early stage. 3. The maintenance ability of the space for the GBR very was excellent. 4. The titanium mesh the tissue-integration was superior the wound fixation ability excellent. 5. The demineralized freeze-dried bone did not promote the bone regeneration. 6. With the lapse of time, formation quantity of the bone some it increased, it increased quantity very it was feeble. Within the above results, the titanium mesh for the guided bone regeneration was excellent, the dεmineralized freeze-dried bone confirmed does not promote bone regeneration.