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양극화 타이타늄 표면처리가 골모세포 증식에 미치는 영향
허인식,박준봉,권영혁,허익,김형선,조병원,조원일,Hur, Yin-Shik,Park, Joon-Bong,Kwon, Young-Hyuk,Herr, Yeek,Kim, Hyung-Sun,Cho, Byung-Won,Cho, Won-Il 대한치주과학회 2003 Journal of Periodontal & Implant Science Vol.33 No.3
The surface characteristics of titanium have been shown to have an important role in contact ossseointegration around the implant. Anodizing at high voltage produces microporous structure and increases thickness of surface titanium dioxide layer. The aim of present study was to analyse the response of rat calvarial osteoblast cell to commercially pure titanium and Ti-6A1-4V anodized in 0.06 mol/l ${\beta}$-glycerophosphate and 0.03 mol/l sodium acetate. In this study, rat calvarial osteoblasts were used to assay for cell viability and cell proliferation on the implant surface at 1,2,4,7 days. 1. Surface roughness was 1.256${\mu}m$ at 200V, and 1.745${\mu}m$ at 300V. 2. The thickness of titanium oxide layer was increased 1 ${\mu}m$ with the increase of 50V. 3. The proliferation rate of osteoblastic cells was increased with the increase of the surface roughness and the thickness of titanium oxide layer. 4. There was no difference in cell viability and cell proliferation between commercially pure titanium and Ti-6A1-4V anodized at the same condition. In conclusion, the titanium surface modified by anodizing was biocompatible, produced enhanced osteoblastic response. The reasons of enhanced osteoblast response might be due to reduced metal ion release by thickened and stabilized titanium dioxide layer and microporous rough structures.
허인식,권영혁,이만섭,박준봉,허익,Hur, Yin-Shik,Kwon, Young-Hyuk,Lee, Man-Sup,Park, Joon-Bong,Herr, Yeek 대한치주과학회 1999 Journal of Periodontal & Implant Science Vol.29 No.1
The purpose of this study was to compare the clinical results of guided tissue regeneration(GTR) using a resorbable barrier manufactured from an copolymer of polylactic acid (PLA) and polylaetic-glycolic acid(PLGA) with those of nonresorbable ePTFE barrier. Thirty two patients(25 to 59 years old) with one radiographically evident intrabony lesion of probing depth ${\geq}$6mm participated in a 6-month controlled clinical trial. The subjects were randomly divided into three independent groups. The first group(n=8) received a ePTFE barrier. The second group (n=12) received a resorbable PLA/PLGA barrier. The third group (n=12) received a resorbable PLA/PLGA barrier combined with an alloplastic bone graft. Plaque index (PI), gingival index(GI), probing depth(PD), gingival recession, clinical attachment level(CAL), and tooth mobility were recorded prior to surgery and at 3, 6 months postsurgery, Statistical tests used to analyze these data included independent t-test, paired t-test, one-way ANOVA. The results were as follows : 1. Probing depth was significantly reduced in all groups at 3, 6 months postsurgery and there were not significant differences between groups. 2. Clinical attachment level was significantly increased in all groups at 3, 6 months postsurgery and there were not significant differences between groups. 3. There were not significant differences in probing depth, clinical attachment level, gingival recession, tooth mobility between second group (PLA/PLGA barrier) and third group (PLA/PLGA barrier combined with alloplastic bone graft) 4. Tooth mobility was not significantly increased in all groups at 3, 6 months postsurgery and there were not significant differences between groups. In conclusion, PLA/PLGA resorbable barrier has similar clinical potential to eP'IFE barrier in GTR procedure of intrabony pockets under the present protocol.
황인식(Insik Hwang),김도형(Dohyoung Kim),허승진(SeungJin Heo),정재일(Jay Il Jeong) 한국자동차공학회 2010 한국자동차공학회 학술대회 및 전시회 Vol.2010 No.11
This paper presents an openness evaluation method of sunroof systems. Recently, panorama type sunroof systems that adopt top-sliding mechanism and are wider than the in-slidng types are very popular to consumers since the higher openess of the panorama sunroof systems is very attractive. In this study, an openness index is defined to evaluate the openness and to improve the design of the sunroof systems . In consideration of ergonomics, the size, position, and shape of the sunroof window are studied as main factors for the openness. The openness of the several sunroof systems is checked by experiments.
전기적 자극이 배양 두개관 골세포의 석회화에 미치는 영향에 관한 연구
박준봉,허인식,이혜자,최영철,Park, Joon-Bong,Hur, In-Sik,Lee, Hye-Ja,Choi, Young-Chul 대한치주과학회 1997 Journal of Periodontal & Implant Science Vol.27 No.4
To date, various clinical procedures have been used to restore periodontal apparatus destroyed by periodontal disease. And then, many experimental approaches have been proceeded to develop treatment methods to promote periodontal regeneration. Mechanical, chemical treatments to enhance the attachment of periodontal tissue cells as changing the physical properties of root surfaces, bone graft procedure, and treatments for guided tissue regeneration have been used for periodontal regeneration. However, recent studies have revealed that biologic factors such as growth factors promote biologic mechanism associated with periodontal regeneration. This study was done to enucleate how ELF stimulus affect the periodontal regeneration. We can have following conclusions from this experimental results. The influence of low frequency(ELF) electric stimulus (30Hz at $lO{\mu}A$) known to promote bone formation in vivo, was evaluated for its ability to affect bone cell function in vitro. After 12 hour exposure of ELF stimulus at most appropriate densities ($5{\times}10^4\;cells/cm^2$) to increase osteoblastic cells normally, rat calvarial cells were incubated for 60 hours were used in this study. We have found ELF stimulus suppress calvarial cell proliferation and the ability of protein synthesis, enhance the alkaline phosphatase activity significantly.