제Ⅱa형 다발성 내분비선종증(MEN type Ⅱa) 3예

박제훈(Je Hoon Park),이해경(Hae Kyung Lee),남석진(Seok Jin Nam),정재훈(Jae Hoon Jung),이명식(Myung Shick Lee),양정현(Jung-Hyun Yang) 대한외과학회 2000 Annals of Surgical Treatment and Research Vol.59 No.1

소련의 경제성장정책 전환과 시베리아 개발

박제훈(Jae Hoon Park) 한양대학교 아태지역연구센터 1985 중소연구 Vol.9 No.2

천마 추출물을 처리한 간암 세포에서 Bcl-2, Bax 및 AMPKα 발현과 세포 자멸사에 미치는 영향 분석

박재현(Jae Hyun Park),강민호(Min Ho Kang),홍지우(Ji Woo Hong),김소희(So Hee Kim),황윤선(Yoon Seon Hwang),박제훈(Jae Hoon Park),김진우(Jin Woo Kim) 한국약용작물학회 2022 한국약용작물학회지 Vol.30 No.5

Background: This study was carried out to investigate the anticancer effects of Gastrodia elata Blume extract (GEE) in human hepatoma-derived Hep3B cells by apoptotic cell death. Methods and Results: To evaluate the anticancer effect, a Hep3B cell viability assay and a real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) on GEE were performed, which was obtained extracted by ultrasound-assisted extraction and then used to treat the Hep3B cells. Cell viability was inhibited to 48.2% (4.0 ㎎/㎖). To identify anticancer mechanisms using qRT-PCR, mRNA expressions of B-cell lymphoma-2, its associated Bax protein, and 5’-AMP-activated protein kinase were examined with a comparative △C<SUB>T</SUB> method. A qualitative analysis of the bioactive compounds in GEE was coducted using liquid chromatography tandem mass spectrometry, gastrodin and phloretin were identified as the two major compounds. Conclusions: These findings demonstrate that GEE can decrease liver cancer cell viability and control apoptosis gene expression to have anticancer effects, GEE could be employed as a natural anticancer agent with less side effects than those of traditional anticancer medications.

간세포구상체의 냉동보존 후 냉동보존액에 따른 세포 생존도와 기능에 대한 연구

박재범(Jae Berm Park),박제훈(Je Hoon Park),이광웅(Kwang-Woong Lee),우돈희(Don Hee Woo),김성주(Sung-Joo Kim),조재원(Jae-Won Joh),이석구(Suk-Koo Lee) 대한외과학회 2003 Annals of Surgical Treatment and Research(ASRT) Vol.65 No.3

반응표면분석법을 이용한 김으로부터 생리활성물질 생산증대를 위한 초음파 추출 조건 최적화

염서희(Suh Hee Yeom),박제훈(Jae Hoon Park),박하영(Ha Young Park),김소희(So Hee Kim),김진우(Jin Woo Kim) 한국산학기술학회 2023 한국산학기술학회논문지 Vol.24 No.8

본 연구에서는 김으로부터 총 폴리페놀 함량(total polyphenol content, TPC), 총 플라보노이드 함량(total flavonoid content, TFC) 및 항산화 활성(radical scavenging activity, RSA)과 같은 생리활성물질의 최적 추출 조건을 확립하는 것이 목적이며 추출 시간, 추출 온도 및 에탄올 농도를 주요 독립변수로 설정하였다. 주요 독립변수의 추출 조건은 반응 표면 분석법의 일종인 중심합성설계를 이용하였으며 초음파 추출을 위한 17개의 독립변수 조건을 설정하였다. 이때 추출 시간 31.0 분, 60.3℃ 및 에탄올 60.0%가 최적 추출 조건으로 설정되었으며 예측값은 TPC 4.44 mg GAE/g DM, TFC 1.25 mg QE/g DM과 RSA 43.5%로 예측되었고, 검증 실험을 진행하였을 때 실험 값이 유의적으로 차이가 나지 않는 것으로 확인되었다. 따라서 최적 추출 조건을 통해 김으로부터 생리활성물질 추출이 증대될 수 있음이 사료되며 향후 식품 및 의약품 산업에 생리활성 물질로서 활용 가능하고 상업화에 효과적인 추출 조건이 확립되었다고 예상된다. In this study, the extraction conditions of bioactive compounds, such as total polyphenol ontent (TPC), total flavonoid content (TFC), and radical scavenging activity (RSA), from Porphyra tenera were optimized using response surface methodology. A central composite design was employed to establish 17 distinct conditions for ultrasound-assisted extraction (UAE), with the extraction time, extraction temperature, and ethanol concentration as the key independent variables. Under the optimal UAE parameters of 31.0 min, 60.3℃, and 60.0% ethanol, the study achieved consistent predicted and experimental values of 4.44 mg GAE/g DM for TPC, 1.25 mg QE/g DM for TFC, and 43.5% for RSA. Overall, this study showed that ultrasound-assisted extraction under optimized conditions could effectively extract bioactive compounds from Porphyra tenera, which may have potential applications in the food and pharmaceutical industries. Overall, this study showed that ultrasound-assisted extraction under optimized conditions could effectively extract bioactive compounds from Porphyra tenera, which may have potential applications in the food and pharmaceutical industries.

소아 간이식에서 Posttansplant Lymphoproliferative Disorder (PTLD): 삼성서울병원의 경험

최연호,이석구,서정민,조재원,김성주,이광웅,박제훈,고영혜,권기영,Choe, Yon-Ho,Lee, Suk-Koo,Seo, Jeong-Meen,Joh, Jae-Won,Kim, Sung-Joo,Lee, Kwang-Woong,Park, Je-Hoon,Ko, Young-Hye,Kwon, Ki-Young 대한소아소화기영양학회 2003 Pediatric gastroenterology, hepatology & nutrition Vol.6 No.1

목 적: EBV의 초감염과 면역억제의 강도는 소아 간이식에서 PTLD 발생의 주요 위험인자로 알려져 있다. 삼성서울병원에서 6년간 경험한 PTLD 5례의 임상양상을 분석해보고자 한다. 대 상: 1996년부터 2002년 6월까지 41례의 소아간이식 환아를 대상으로 하였는데 이중 7명이 사망하였고, PTLD로 사망한 1명을 포함한 35명을 대상으로 분석하였다. 방 법: 공여자의 EBV 항체가 양성이고 수혜자가 음성일 경우 고위험군으로, 그 외의 경우를 저위험군으로 정의하였다. 위험군 분류, 면역억제제 종류, 간이식이 행하여진 나이, 수술 후 PTLD 진단까지의 기간, 수술 후 EBV 항체의 양전 시기, 거부반응에 대한 치료 여부, PTLD의 증상들인 발열, 설사, 빈혈, 장출혈 식욕부진 저알부민혈증 등의 발현 여부를 후향적으로 조사하였다. 결 과: 1) PTLD는 전체 소아 간이식 환아 41명 중 5명에서 발생하였다(12.2%). 고위험군은 16명으로 이 중 5명(31.3%)에서 PTLD가 발생하였고 저위험군 19명중에서는 한 명도 발생하지 않았다. 2) 사망례를 제외한 4명은 tacrolimus로 면역억제 치료를 받았으며 수술 당시 나이는 평균 10.8개월이었고 수술 후 PTLD 진단까지 평균 9.8개월이 걸렸 다. EBV 양전 시기는 수술 후 평균 6개월이었다. 3) 사망례를 포함한 5명 중 3명에서 PTLD 진단 이전에 간이식 거부반응이 있었고 스테로이드 pulse 치료 등 면역억제를 증가시키는 치료를 행하였었다. 4) 한 명에서 쉰 목소리 증상이 발견되어 조직검사 상 후두 PTLD와 장 PTLD로 진단하였고 나머지 네 명은 모두 혈변이 있었으며 대장 조직검사 상 장 PTLD로 진단하였다. 5) 빈혈과 저알부민 혈증은 모든 환아에서 나타나고 있었고 발열, 설사, 장출혈이 5명 중 4명에서 보였으며 식욕부진은 5명 중 3명에서 나타났다. 결 론: 10%가 넘는 발병률을 보이는 PTLD는 소아 간이식 후 매우 중요한 합병증으로서 특히 EBV의 고위험군에서 31%에 이르는 발병률을 가지고 있는 바 이들에 대하여 EBV 상태와 면역억제 유지에 대한 철저한 대비가 있어야 할 것으로 생각된다. 특히 빈혈, 저알부민혈증, 발열, 설사, 장출혈 등이 동반할 때에는 PTLD를 의심해 볼 수 있다. PTLD의 공통적인 발병양상으로는 첫째, 공여자가 EBV 양성이고 수혜자가 음성이면서, 둘째, EBV가 수술 후 6개월 경 양전되며, 셋째, 1세 전후에 수술을 시행하고 넷째, 간이식 거부반응을 겪으면서 면역억제의 강도가 높아졌을 경우 등이다. Purpose: In a retrospective study for the pediatric patients who underwent liver transplantation in the past 6 years at Samsung Medical Center, the clinical features of 5 patients with posttransplant lymphoproliferative disorder (PTLD) were analyzed. Methods: Between June 1996 and June 2002, 41 pediatric patients underwent liver transplantation. Seven of them died in the postoperative period. Thirty-five including one patient who died of PTLD were finally reviewed. Patients were divided into two groups: high risk group, EBV naive recipients of EBV-positive grafts; low risk group, the patients other than those in high risk group. The authors reviewed age at operation, immunosuppressive agent, postoperative duration until diagnosis, postoperative duration until EBV seroconversion, presence of treatment against rejection, and presenting symptoms of PTLD. Results: Five of 41 patients (12.2%) developed PTLD. All of them belonged to high risk group, and the incidence of PTLD in high risk group was 31.3% (5/16). The mean age at operation was 10.8 months old and the mean duration between operation and diagnosis for PTLD was 9.8 months. Primary EBV infection developed after a median of 6 months after transplantation. One patient was diagnosed as laryngeal and gastrointestinal PTLD and the other four, gastrointestinal PTLD. The following symptoms and signs were seen in the patients: anemia (100%), hypoalbuminemia (100%), fever (80%), diarrhea (80%), gastrointestinal bleeding (80%), and anorexia (60%). Conclusion: PTLD is one of the major complications after pediatric liver transplantation, especially in the group of high-risk recipients. Anemia, hypoalbuminemia, fever, diarrhea and gastrointestinal bleeding were features that are characteristic of PTLD. The common features of PTLD development were: (i) EBV-positive donors placed into EBV naive recipients, (ii) primary EBV infection about 6 months after transplantation, (iii) young age, about 1 year old at operation, and (iv) the requirement for intensive posttransplant immunosuppression.

장관 기종의 임상적 고찰

민선영(Sun Young Min),김재일(Jae Il Kim),최평화(Pyong Wha Choi),박제훈(Je Hoon Park),허태길(Tae Gil Heo),이명수(Myung Soo Lee),김철남(Chul Nam Kim),장석효(Surk Hyo Chang),김한성(Han Seong Kim) 대한외과학회 2010 Annals of Surgical Treatment and Research(ASRT) Vol.79 No.6

Purpose: Pneumatosis intestinalis (PI) is increasingly being detected in recent years with the more frequent use of computerized tomography (CT). The present study was performed to evaluate the clinico-radiologic characteristic presentation of PI and to determine the prognostic factors for mortality. Methods: Fifteen patients who were diagnosed with PI on CT between June 2000 and May 2010 were retrospectively reviewed. Age, sex, location of PI, presence of portal vein gas, time to diagnosis, American Society of Anesthesiologists (ASA) classification, Acute Physiology And Chronic Health Evaluation Ⅱ (APACHE Ⅱ), acidosis, shock, and other associated findings were analyzed for their association with outcome. Results: Fifteen patients (7 males and 8 females, average age, 60.3 years) were diagnosed with PI. Mortality rate was 47% (7 patients). The mortality rate in patients with septic shock, APACHE Ⅱ score (18), acidosis (pH<7.36) were all 100%, 87%, 100%, respectively (P<0.05). Age, sex, location of PI, portal vein gas, time to diagnosis, ASA classification, associated findings did not show statistical difference. Conclusion: Shock, APACHE Ⅱ score (18) and acidosis were associated with high mortality in patients with PI. So, more intensive management and interest are recommended in patients with shock, APACHE Ⅱ score (18), and acidosis due to PI.

외상성 췌장 손상의 임상 결과 및 예후인자

이홍태 ( Hong Tae Lee ),김재일 ( Jae Il Kim ),최평화 ( Pyong Wha Choi ),박제훈 ( Je Hoon Park ),허태길 ( Tae Gil Heo ),이명수 ( Myung Soo Lee ),김철남 ( Chul Nam Kim ),장석효 ( Surk Hyo Chang ) 대한외상학회 2011 大韓外傷學會誌 Vol.24 No.1

Purpose: Even though traumatic pancreatic injuries occur in only 0.2% to 4% of all abdominal injuries, the morbidity and the mortality rates associated with pancreatic injuries remain high. The aim of this study was to evaluate the clinical outcomes of traumatic pancreatic injuries and to identify predictors of mortality and morbidity. Methods: We retrospectively reviewed the medical records of 26 consecutive patients with a pancreatic injury who underwent a laparotomy from January 2000 to December 2010. The data collected included demographic data, the mechanism of injury, the initial vital signs, the grade of pancreatic injury, the injury severity score (ISS), the revised trauma score (RTS), the Glasgow Coma Scale (GCS), the number of abbreviated injury scales (AIS), the number of associated injuries, the initial laboratory findings, the amount of blood transfusion, the type of operation, the mortality, the morbidity, and others. Results: The overall mortality rate in our series was 23.0%, and the morbidity rate was 76.9%. Twenty patients (76.9%) had associated injuries to either intra-abdominal organs or extra-abdominal organs. Two patients (7.7%) underwent external drainage, and 18 patients (69.3%) underwent a distal pancreatectomy. Pancreaticoduodenectomies were performed in 6 patients (23.0%). Three patients underwent a re-laparotomy due to anastomosis leakage or postoperative bleeding, and all patients died. The univariate analysis revealed 11 factors (amount of transfusion, AAST grade, re-laparotomy, associated duodenal injury, base excess, APACHE II score, type of operation, operation time, RTS, associated colon injury, GCS) to be significantly associated with mortality (p<0.05). Conclusion: Whenever a surgeon manages a patient with traumatic pancreatic injury, the surgeon needs to consider the predictive risk factors. And, if possible, the patient should undergo a proper and meticulous, less invasive surgical procedure. (J Korean Soc Traumatol 2011;24:1-6)

해당화 추출물의 주요물질 분석에 따른 폴리페놀 함량 및 항산화 활성 탐색

김준희(Jun Hee Kim),황윤선(Youn Sun Hwang),박제훈(Jae Hoon Park),강민호(Min Ho Kang),오예솔(Ye Sol Oh),김진우(Jin Woo Kim) 한국생명과학회 2022 생명과학회지 Vol.32 No.11

해당화로부터 초음파 추출을 이용한 기능성 물질 생산 및 이를 통한 화장품용 소재 발굴을 위해 HPLC와 HPLC-MS/MS를 이용하여 추출물의 주요물질을 탐색하고 항산화 활성에 기반한 기능성을 평가하였다. 해당화 추출물의 HPLC 분석을 통해 해당화 추출물 내 갈산과 에피갈로카테킨 갈레이트가 각각 17.4와 4.35 mg/g 포함됨을 확인하었으며 HPLC-MS/MS 분석을 통해 해당 물질이 해당화 추출물 내 주요물질임을 재확인하였다. 항산화 활성을 평가하기 위해 TPC, TFC와 RSA를 분석한 결과 72.3±1.5 mg GAE/g DM, 11.2±0.7 mg QE/g DM과 87.9±0.3%로 확인되었으며 HPLC 분석을 통해 확인된 갈산과 에피갈로카테킨이 해당화의 TPC와 TFC에 기여하는 주요물질임이 확인되었다. 주요물질의 RSA를 측정한 결과 갈산과 에피갈로 카테킨 갈레이트가 해당화 추출물의 항산화 활성에 기여하는 주요물질임이 재차 확인되었다. 따라서, 해당화 추출물이 우수한 생리활성 효능을 가지는 갈산과 에피갈로카테킨 갈레이트의 함량이 높고 이에 따른 높은 폴리페놀 함량과 함께 우수한 항산화 활성이 확인됨에 따라 건강기능성 식품과 화장품 천연 항산화제 소재로써 부가가치를 창출할 수 있을 것으로 사료된다. The purpose of this study was to evaluate the antioxidant properties of Rosa rugosa extract and to identify which of its components are responsible for these properties. Reactive oxygen species play an important role in diseases such as cancer, arteriosclerosis, and heart disease as a consequence of increased metabolic rates, gene mutations, and relative hypoxia. Therefore, the antioxidant effect of R. rugosa extract was confirmed by HPLC, HPLC-MS/MS, the total polyphenol content, the total flavonoid content, and the radical scavenging activity. HPLC and HPLC-MS/MS analyses were conducted to identify and quantify the main components of the R. rugosa extract. Gallic acid and epigallocatechin gallate were identified as the main components, with 17.4 and 4.35 mg/g dry matter (DM), respectively. The antioxidant activity of R. rugosa extract was evaluated based on its total polyphenol content, total flavonoid content, and radical scavenging activity, which were 72.3 mg gallic acid equivalent/g DM, 11.2 mg quercetin equivalent/g DM, and 87.9%, respectively. The radical scavenging activities of the main components, gallic acid and epigallocatechin gallate, were 80.5% and 89.7%, respectively. Therefore, R. rugosa has a high polyphenol content and antioxidant activity, and it can be used as a natural antioxidant in food, cosmetics, and pharmaceuticals.

비접촉 암호 분석용 루프 프로브 설계 및 전자파 신호 측정

최종균(Jong-Kyun Choi),김채영(Che-Young Kim),박제훈(Jea-Hoon Park),문상재(Sang-Jae Moon) 한국전자파학회 2007 한국전자파학회논문지 Vol.18 No.10

본 논문은 스마트카드에서 방사되는 전자파를 이용한 암호 분석용 소형 루프 프로브 설계와 프로브에 유기된 전자파 신호의 분석에 관한 연구이다. 암호 분석용 프로브는 일반적인 전자장 측정용 프로브와는 다르다. 그 이유는 제시된 프로브의 목적은 암호 장비의 비밀 키 해독에 필요한 정보를 얻기 위함이기 때문이다. 스마트카드에서 발생된 파형을 분석하여서 전자파 공격을 시도하기 때문에 프로브에 유기된 전압의 파형과 IC 칩에서 발생된 파형은 가능한 한 서로 동일해야 한다. 그 목적을 위해서 전자파를 이용한 암호 분석, 신호원의 근사모델링, 암호 분석용 프로브의 특징, 전자파 신호 측정 그리고 프로브의 교정에 대한 검토가 필요하다. 스마트카드 칩의 소비 전력 신호와 제안된 프로브를 이용한 전자파 신호를 측정하고 두 신호를 EMA의 관점에서 비교하였다. ARIA 알고리즘을 적용하여 제안된 비접촉 암호 분석용 프로브의 적합성 여부를 실험적으로 확인하였다. In this paper, a study has been performed on the design of small loop probe and analysis of induced electromagnetic wave signal from a smartcard for contactless cryptographic analysis. Probes for cryptographic analysis are different from conventional EM probes, because the purpose of proposed probe is to obtain the information for secret key analysis of cryptographic system. The waveform of induced voltage on probe must be very close to radiated waveform from IC chip on smartcard because electromagnetic attack makes an attempt to analyze the radiated waveform from smartcard. In order to obtain secret key information, we need to study about cryptographic analysis using electromagnetic waves, an approximate model of source, characteristic of probe for cryptographic analysis, measurement of electromagnetic waves and calibration of probes. We measured power consumption signal on a smartcard chip and electromagnetic wave signal using proposed probe and compared with two signals of EMA point of view. We verified experimently the suitability of the proposed small loop probe for contactless cryptographic analysis by applying ARIA algorithm.