Dextran sulfate sodium으로 유발된 큰창자염에 대한 조직학적 연구

박인식(In-Sick Park) 대한해부학회 2006 Anatomy & Cell Biology Vol.39 No.1

큰창자염(대장염, colitis)은 큰창자 점막에 다발성 궤양을 동반한 원인 불명의 질환으로 과거에는 유럽 및 북미지역에 국한된 질환으로 알려졌으나 최근에는 우리나라에도 발생빈도가 점점 증가추세를 나타내고 있다. 큰창자염의 원인은 아직 잘 알려지지 않고 있으며 증상은 설사, 복통, 체중감소 등 여러 가지가 있다. 본 연구는 흰쥐에 5% dextran sulfate sodium (이하 DSS, MW; 4,000)을 5일 동안 음용시켜, 인위적으로 궤양성 큰창자염을 유발시킨 후 다시 7일 동안 생수를 음용시켜 재생과정에서의 큰창자상피의 조직학적인 변화를 살펴보았다. 본 연구의 실험군에서 심한 체중감소와 큰창자상피에서 창자샘의 짓무름, 표면상피세포의 탈락들을 관찰할 수 있었다. 그리고 창자샘의 감소로 인하여 창자샘에서 관찰되는 점액질도 감소하였다. 회복군에서는 설사가 줄고 체중이 증가하며 큰창자상피의 창자샘의 재생현상을 관찰할 수 있었다. 조직의 재생정도를 알 수있는 Ki 67 면역염색은 정상군에서 창자샘의 아래쪽 1/3에 국한되어 나타났으나 회복군에서는 재생되는 창자샘의 표면전층에서 강한 양성반응을 나타내었다. 그러므로 본 연구에서 사용된 DSS는 인위적인 궤양성 큰창자염을 연구하는데 유용하며 큰창자염의 회복시 창자샘의 왕성한 재생활동을 관찰할 수 있었다. Inflamatory bowel disease is the general medical terminology on chronic inflammatory illness of unknown origin, but it is considered that environmental, genetical, immunological factors may develop this chronic disease. I examined the histological changes on the experimental ulcerative colitis induced by dextran sulfate sodium (DSS) in rats. Experimental colitis was induced with 5% DSS in the drinking water for 5days in rats (experimental group). And the repair group were treated with 5% DSS for 5days and with pure water after 7days in rats. In experimental group, there are many inflammatory finding in colon of rat which contained loss of body weight, crypt erosion, recruitment of inflammatory cells, submucosal edema. In repair group, the inflammation was recovered that the body weight was incerased, the crypt was recovered. And Ki 67 immunoreaction were restricted lower 1/3 crypt in normal group but positive Ki 67 reaction appeared in the repaired all region. In this study, DSS was induced experimental colitis and the colitis was repaired when we stoped DSS supply. And the Ki 67 during repaired period were overproduction.

실험적으로 염증이 유발된 흰생쥐의 큰창자에서 염증관련물질들의 면역조직화학적 변화 연구

박인식(In-Sick Park) 대한해부학회 2006 Anatomy & Cell Biology Vol.39 No.6

염증성 창자질환(inflammatory bowel disease)은 창자에 만성적인 염증을 일으키는 질환으로 아직 그 정확한 원인과 병리상태가 알려지지 않고 있다. 본 연구는 생쥐에서 dextran sulfate sodium (DSS)를 이용하여 인위적으로 염증성 창자질환을 유발시킨 후 큰창자상피의 염증관련 물질들의 면역조직학적인 변화를 살펴보았다. 본 연구는 생쥐에 3% DSS (mw; 40,000)를 5일 동안 음용시켜, 인위적으로 큰창자염을 유발시켰다. 큰창자염이 유발된 생쥐의 큰창자를 보면, 육안해부학적으로 큰창자의 직경이 증가하고 체중이 감소하였으며 조직학적 소견으로는 창자샘의 짓무름, 표면상피세포 탈락, 고유판내에 염증세포 침착, 점막밑조직에 많은 염증세포, 점막밑조직의 부종 등의 현상을 보이고 있었다. 면역조직화학적으로 Cyclooxygen ase1 (COX-1)은 별다른 변화를 보이지 않았으나 실험군에서 COX-2의 면역반응이 증가하였으며, Heat shock protein 70 (HSP-70)의 면역반응도 증가하였다. Macrophage387 (MAC387)의 면역양성세포수가 증가하였으며 proliferating cell nuclear antigen (PCNA)의 반응성도 점막전체에서 증가하였다. 그리고 TUNEL 반응결과 apoptosis도 증가하였다. 이상의 결과에서 큰창자염이 유발되면 염증현상과 더불어 조직방어기작이 일어나며 조직의 재생관련물질도 관찰할 수 있었다. The experimental ulcerative colitis is chronic inflammatory illness in colon, which didn’t reveal the exact reason and pathophysiological situation. In this study, the colitis was induced by 3% DSS (mw; 40,000) for 5 days in mouse which was resemble to inflammatory bowel disease in human, and immunohistochemical changes were observed in the mucosa. In inflammatory group, thickness of the colon was increased and the length of colon was shorter than that of the normal group and the body weight decreased. In microscopic aspect, the crypt erosion, many inflammatory cells and submucosal edema were occurred. In immunohistochemical study, the immunity of COX1 in the inflammatory group was not changed to comparing the normal group, but COX2 immunoreactivity was increased than the normal group, HSP70 immunoreactivity were also increased than the normal group. MAC387 which used to detect the macrophage was increased than the normal group and PCNA immunoreactivity were increased along to the mucal layer. And the number of apoptosis cells detected by TUNEL was increased. In these results, the experimental colitis revealed the tissue defense mechanism as well as inflammatory system and observed the stuffs related to the regeneration.

뇌실곁핵 (paraventricular nuclei)에서 탈수자극에 의한 neuropeptide 면역반응양상 변화

박인식(In-Sick Park),김진택(Jin-Taek Kim) 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.1

Dehydration induced an increase in plasma osmotic pressure that causes the release of the neurohypophysial hormone (Vasopresin, Oxytocin) which are synthesized in neurons of the paraventricular (PVN) and supra optic (SON) nuclei in the hypothalamus. On the other hand, PVN which plays an important role as an integration site for the neuroendocrine and autonomic nervous system neurons responded to osmotic stimulation. In this experiment, we studied that the change of several neuropeptidies (AVP: arginine vasopressin, CRF: corticotrophin releasing factor, GAL: galanin, NT: neurotensin. NPY: neuropeptide Y) immunoreactivity in the PVN according to the dehydration. The body weight of the rats decreased during dehydration and various changes were detected in hypothalamic neuropeptidies immunoreactivity.Our results show that: 1. Dehydration significantly increased AVP, CRF and GAL immunoreactivity in the PVN. 2. Dehydration slowly decreased NT immunoreactivity in the PVN. 3. NPY immunoreactive cell bodys were appeared during dehydration which did not observed in PVN at normal group. 인위적인 탈수자극이 뇌실곁핵 (paraventricular nuclei; PVN)에 존재하는 여러 신경전달물질 (arginine vasopressin; AVP, corticotropin releasing factor; CRF, galaine; GAL, neurotensin; NT, neuropeptide Y; NPY)의 분포양상에 어떤 영향을 미치는지를 조사하기 위해 면역조직화학적 기법을 이용하여 본 연구를 시행하였다. 실험동물은 체중 200 g 내외의 숫컷 흰쥐를 사용했으며 대조군과 7일동안 식수의 공급을 중지시킨 탈수군으로 나누어 시행한 결과 다음과 같은 결과를 얻었다. 탈수기간동안 실험동물의 체중은 급격하게 감소하여 탈수 7일군에서는 약 46%의 체중감소를 나타내었다. 1. 탈수자극에 영향을 받는다고 알려진 AVP, CRF 그리고 GAL 면역반응 양성신경세포와 신경섬유는 뇌실곁핵에서 실험 기간동안 계속하여 증가하는 경향이 관찰되었다. 2. 뇌실곁핵에서 NT 면역양성 세포체와 신경섬유는 탈수기간 동안 점차적으로 감소하였으며 7일군에서는 급속히 감소하 여 나타났다. 3. NPY 면역양성신경세포는 정상군에서는 신경섬유만이 나타났는데 탈수자극 7일군에서는 뇌실곁핵의 거대신경세포지역 (magnocellular part)에서 많은 신경세포체가 관찰되었다.

중간대뇌동맥 폐쇄가 해마형성체의 피라미드 신경세포에 미치는 영향

박인식(In Sick Park) 대한해부학회 2001 Anatomy & Cell Biology Vol.34 No.2

뇌혈관폐쇄 후 재관류를 시행한 표본에서 뇌세포의 손상을 관찰하기 위하여, 흰쥐에서 속목동맥속으로 단일가닥의 나 이론실을 넣어 실험적으로 중간대뇌동맥을 폐쇄하여 1시간 동안 뇌경색을 유발시키고 그 후 재관류시킨 표본을 이용하여 뇌의 변화와 해마형성체내의 피라미드 신경세포의 변화를 살펴보았다. 본 연구에서는 다음과 같은 결과를 얻었다. 1) 본 연구를 위한 시술은 약 20~30분 정도가 소요되었는데 시술받은 동물의 약 30%만이 신경학적인 조건에 적합하 여 실험에 사용할 수 있었다. 2) TTC (triphenyl tetrazolium chloride; Tetrazolium Red) 염색결과 재관류 72시간 표본에서 전체 뇌면적의 약 23.9% 정도의 상해부위를 관찰할 수 있었다. 3) 뇌부종의 정도를 측정한 결과 왼쪽 뇌가 오른쪽 뇌에 비해 약 17% 정도 커져있었다. 4) 해마형성체의 피라미드 신경세포는 실험군에서 많이 위축되는 등 괴사소견을 나타내고 있었다. 5) 해마형성체의 생존세포수를 조사한 결과 1mm2 내에 평균 93개이던 세포가 실험 3일군에서는 평균 21개, 실험 7일군 에서는 평균 3개로 나타났다. 그러므로 일시적인 중간대뇌동맥의 폐쇄가 뇌조직의 사망에 직접적으로 영향을 미치며, 해마형성체의 피라미드 신경세 포의 지연성 신경세포사망에 영향을 미침을 알 수 있었다. In order to study damages on brain cells upon occlusion and reperfusion, brain infarction was induced by insertion of a single nylon thread, through the internal carotid artery, into a middle cerebral artery. After occlusion of 1 hr and reperfusion for variable duration, brain slices were used to observe changes in the brain morphology and in the pyramidal neurons of the himppocampal formation. In this study we found following results. 1) The operation took 20~30 min and about 30% of the operated animals were suitable for studying neurological aspects. 2) The TTC stain showed that about 23.9% of the total brain area was damaged in the 72 hr-reperfusioned sample. 3) The degree of brain edema was larger in the left hemisphere (damaged side) than in the right one (contralateral undamaged side). 4) Pyramidal cells of the damaged hippocampal formation showed features of necrosis such as shrinkage, large vacuole, swelling, and cell debris. 5) The numbers of survived cells per mm2 of the hippocampal formation were 93 in the undamaged animal, and 23 and 3 in the 3 and 7 days after reperfusion, respectively.

방풍당귀음(防風當歸飮)이 중대뇌동맥 폐쇄 후 재관류 모델에 미치는 영향 -육안.광학현미경 소견-

홍천표,박인식,신길조,이원철,정승현,Hong, Cheon-Pyo,Park, In-Sick,Shin, Gil-Cho,Lee, Won-Chul,Jeong, Sung-Hyun 대한한방내과학회 2000 大韓韓方內科學會誌 Vol.21 No.2

To evaluate the effect of Bangpungdangkwi-eum extracts on reperfusion following the middle cerebral artery occulsion in Sprague Dawley rats, the neuron protection effect were investigated through examining the size of cerebral infarction, cerebral edema, and the morphologic change of neuron. The results were obtained as follows; 1. The size of cerabral infarction in sample group is significantly decreased compared with that in control group. Sample group has approximately 17% cerebral infarction parts induced by ischemia in cerebrum while control group has approximately 22%. 2. The volume of cerebral edema in sample group is significantly decreased compared with that in control group. The volumn in sample group is increased by approximately 4.4% compared with that in normal group while that in control group is increased by approximately 7.7%. 3. The optical microscopic examination reveals that the damage of neurons in the ischemic parts and CA1 and CA3 region of hippocampus in the same side of the ischemic parts was the most high and the damage in sample group is decreased compared with that in control group.

거풍지보단(祛風至寶丹)이 Mongolian Gerbil의 가역성 전뇌허혈 모델에 미치는 영향

정완우,박인식,신길조,이원철,정승현,Jeong, Wan-Woo,Park, In-Sick,Shin, Gil-Cho,Lee, Won-Chul,Jeong, Sung-Hyun 대한한방내과학회 2001 大韓韓方內科學會誌 Vol.22 No.2

Objectives : The purpose of this investigation is to evaluate the effect of Geupoongjibo-dan Extracts on Reversible Forebrain Ischemia in Mongolian Gerbils. Methods : The change rate of water content in cerebral tissues, the numercal change of the CA1 pyramidal neuron in the hippocampus, the change of delayed neuronal death(necrosis apoptosis) through light microscopy, the reactivity change of glycoprotein in neuronal membrane and the ultrastructural change of pyramidal neuron through electron microscopy caused by dalayed neuronal death were investigated. Results : 1. The change rate of water content in the normal group showed 78.90% on the third day, and 79.12% on the seventh day after an attack of ischemia. The rate in the control group showed 82.25% and 85.13%, respectively. The rate in the sample group showed a significant decrease: 81.72% and 83.66%. 2. Light microscopy revealed that the cells, continuous and systematic forms in the pyramidal cells of hippocampus, changed into discontinuous and unsystematic forms in the normal group when compared with the control group. The cells were less damaged in the sample group. 3. The mean of the numerical change of the CA1 pyramidal neurons in the hippocampus was 104 in the normal group. The mean of the control group was decreased to 27. The mean of the sample group was 44. 4. TUNEL staining examination reveals that the whole part of the hippocampus of the normal group had negative reactivity. As far as CA1 pyramidal neurons in the hippocampus, the control group had positive reactivity. The sample group was more positive than the control group. 5. Electron microscopy reveals that the ischemic injury of the control group had both necrotic and apoptotic morphology. The sample group was less necrotic, and more apoptotic morphology than the control group. 6. Lectin histochemisrical examination reveals that the normal group had positive reactivity to PNA and SBA in interneuron, and weak positive reactivity to WGA Con A LCA in intercelluar space. The reactivity to PNA and WGA decreased in the control group. The reactivity to PNA and WGA tended to increase in the sample group. Conclusions : The data shows that the effect of Geupoongjibo-dan Extracts on Reversible Forebrain Ischemia in MG is a significant result.

청폐사간탕(淸肺瀉肝湯)이 중대뇌동맥 폐쇄 후 재관류에 미치는 영향

오연환,박인식,신길조,이원철,정승현,Oh, Yean-Hwan,Park, In-Sick,Shin, Gil-Cho,Lee, Won-Chul,Jeong, Sung-Hyun 대한한방내과학회 2001 大韓韓方內科學會誌 Vol.22 No.2

Objectives : The purpose of this investigation is to evaluate the effect of Chungpesagan-Tang Extracts on reversible forebrain ischemia in Sprague-Dawley rats. Methods : the volume of cerebral infarction and edema, the pathohistological change of neurons, the number of survived neurons, neurotransmitters through immunohistochemical methods, proteins connected with neurotransmitters through immunohistochemical methods and the pathohistological change of neurons through electro-microscopy were investigated. From these reseach data, the protection of neurons and the activity of brain cells were examined. Results : 1. The infaction volume of the control group was 23.9%, and that of the sample group was 16%. 2. The brain edema volume of the control group increased by 17% compared to the normal group and that of the sample group increased by 10%. 3. The light microscopy revealed that the neurons in the ischemia-induced area and CA1 area of hippocampus were most heavily damaged and that the sample group was less damaged compared with the control group. Most pyramidal neurons died in 7 days when brain ischemia was induced. 4. The number of survived pyramidal neurons in the CA1 area of the hippocampus were studied. The normal group had 93 neurons/mm, survived the control group(after 3 days) had 21/mm, the control group(after 7 days) had 3/mm and the sample group 33/mm. 5. The immunohistochemical methods revealed that: (1) In the control group, the sensitivity of GABA, NOS, DBH were increased, and those of Synapsin, eEF-$1{\alpha}$ decreased. NOS and DBH had positive reactions in the control group, but negative in the normal group. (2) In thd sample group, the sensitivity of GABA, NOS, DBH were attenuated, and those of NPY, Synapsin, CaMKII, eEF-$1{\alpha}$ increased when compared to the control group. 6. The electro-microscopy revealed that most neurons died by necrosis and some neurons died by apoptosis. Several imflammation cells appeared in the injured area of neurons. The number of neurons in the sample group that died by ischemia decreased. But, the number that died by apoptosis did not significantly change. Conclusions : The data shows that the effect of Chungpesagan-Tang Extracts on reversible forebrain ischemia in Sprague-Dawley rats is significant.

The Inducible form of Heat Shock Protein 70 (Hsp70) is Expressed in the Rat Cerebellar Synapses in Normal Condition

조선정,정재섭,진익렬,정승현,박인식,문일수,Cho Sun-Jung,Jung Jae-Seob,Jin IngNyol,Jung Seung Hyun,Park In Sick,Moon Il Soo Korean Society of Life Science 2005 생명과학회지 Vol.15 No.4

Heat shock protein 70 (HSP70) is a multigene family composed of constitutively expressed members(Hsc70) and stress-inducible members (Hsp70). In the mammalian nervous system, a considerable amount of HSPs is also synthesized under normal conditions suggesting that they play an important role in the metabolism of unstressed cells. In this study we examined the expression of Hsp70 in the synapses of rat cerebellar neurons. Immunohistochemistry using specific antibodies revealed that both Hsp70 and Hsc70 are expressed in the cerebellar tissue, with strongest expression in Purkinje cells followed by granule cells. Neurons in deep cerebellar nuclei were also intensely stained by Hsp70 antibody. Immunocytochemical stainings of cultured cerebellar cells showed that Hsp70 is expressed in both Purkinje and granule cells. The expression was punctate in the soma and along dendritic trees, and the punctae were colocalized with those of PSD95, a postsynaptic marker. Immunoblotting also indicates that Hsp70 is associated with the postsynaptic density fraction. Taken together, our results indicate that the Hsp70 is expressed in cerebellar neurons in normal conditions, and that some are localized in the synapses. 열충격단백질 70 (HSP70)은 복수유전자족으로서 통상적으로 표현되는 Hsc70와 스트레스에 의하여 유도되는 Hsp70가 있다. 포유동물의 신경계통에서는 상당한 량의 HSP70가 정상조건에서도 표현되는 것으로 알려져 있다. 본 연구에서는 흰쥐의 소뇌 세포의 연접에서 Hsp70의 표현에 대한 연구를 하였다. 면역조직화학적으로 소뇌절편을 염색하여 관찰한 결과 Hsp70와 Hsc70 모두 표현되었는데, 소뇌 조롱박세포에서 가장 강하게 표현되었으며, 다음으로 소뇌 과립세포에서 강하게 표현되었다. 또한 깊은소뇌핵의 신경세포들도 강하게 염색되었다. 배양한 P1 소뇌신경 세포를 Hsp70 항체로 염색한 결과 Hsp70는 조롱박세포와 과립 세포에서 모두 표현되었으며, 세포체와 가지돌기를 따라 점박이를 형성하였다. 이들 점 박이들은 PSD95 점박이와 같이 위치하였다. 그리고 PSD 분획을 이용한 면역염색에서도 PSD70이 검출되었다. 본 연구결과는 Hsp70이 정상조건에서도 소뇌신경세포의 연접에 존재함을 의미한다.

The Effects of Yacon (Smallanthus sonchifolius) Extract on Pancreatic Fibrosis in the Rat

Nan Hee Choi(최난희),Jong Bong Kim(김종봉),Jin Teak Kim(김진택),In-Sick Park(박인식) 한국생명과학회 2012 생명과학회지 Vol.22 No.7

야콘(Smallanthus sonchifolius)은 저혈당에 민간요법으로 이용해 오고 있는 구근작물이다. 최근에는 야콘잎이 항산화, 항균, 항진균 활성과 세포보호 기능이 있다고 보고되고 있다. 본 실험에서는 이러한 생리활성을 지니는 야콘잎을 이용하여 흰쥐에 DBTC (8 mg/kg)를 주사하여 췌장염을 유발시킨 후 1% 야콘추출물이 췌장의 섬유화에 미치는 영향을 연구하였다. 유발 21일째 처리군에서 췌장의 실질조직의 많은 부분이 collagen으로 재구성되어 있었으나 유발군에서는 처리군에 비해 현저히 감소되어 있었다. COX-2 발현에서, 대조군은 반응이 나타나지 않거나 매우 약한 반응이었으나, 유발군에서는 14일째 매우 증가되었으며, 특히 21일째는 침윤하고 있는 많은 염증세포에서 COX-2의 발현이 확인되었다. 처리군은 유발군에 비해 발현이 감소되었다. TGF-β1 발현은 21일째 염증세포에서는 유발군이 처리군에 비해 현저한 증가 현상이 나타났으나, 샘꽈리세포에서의 TGF-β1의 발현은 처리군에서 증가되었다. VEGF 발현은 TGF-β1의 발현과 거의 유사한 경향으로 나타났다. 그러므로 야콘추출물이 DBTC로 유도된 췌장염의 섬유화 진행을 억제하는데 매우 효과적임을 확인하였다. Yacon has been used in folk medicines as a medicinal tea for hypoglycemia treatment. In a recent study described herein, antioxidative, antibacterial, antifungal activities, and cell-protective functions of yacon leaves have been reported. To evaluate the effects on fibrosis on pancreatitis, the efficacy of 1% of yacon extract (YE) on dibutyltin dichloride (DBTC) (8 mg/kg)-induced pancreatitis in rats was examined. On the 21st day after the DBTC treatment, a large increase in collagen was observed in the pancreas in the DBTC-treatment group (DT). But this was noticeably decreased with YE. In relation to the expression of COX-2, there was no response or a very weak response in the pancreas of the control group (CON). However, in DT, strong expression of COX-2 was observed in the pancreas on the 14th day, and COX-2 was present in inflammatory cells in the pancreas of the DT, especially on the 21st day. The expression was decreased for YE compared with DT. A remarkable increase in TGF-β1 expression was observed in inflammatory cells in the pancreas in DT on the 21st day, whereas the expression was not found in YE after 21 days. However, on the 21th day, TGF-β1 expression was increased in acinar cells of YE compared with DT. VEGF expression was very similar to the expression of in the pancreas. These results suggest that YE has an inhibitory effect on DBTC-induced pancreatic fibrosis.

야콘 (Smallanthus sonchifolius) 추출물이 Dibutyltin Dichloride로 유발된 췌장염에 미치는 영향

최난희(Nan Hee Choi),최성훈(Seong Hun Choi),임성우(Seong-Woo Lim),박인식(In-Sick Park) 대한해부학회 2007 Anatomy & Cell Biology Vol.40 No.3

야콘은 민간 요법에서 고혈당에 대한 차로 이용되고 있으며, 최근 연구에서, 야콘잎은 항산화, 항균, 항진균 효과 및 세포 보호기능이 있음이 보고되고 있다. 본 연구에서는 야콘 추출물에 의한 췌장염의 효과를 확인해 보기 위하여 DBTC (8 mg/kg)로 유발된 췌장염에서 그 효과를 실험하였다. 췌장염 유발군은 DBTC 투여 21일째 염증관련 세포의 급증과 샘꽈리세포의 괴사가 심해지면서 주변의 결합조직이 매우 증가된 반면, 1% 야콘추출물을 음용한 처리군에서는 유발군에 비해 염증 진행이 매우 억제되어 있었다. HSP27, HSP70, MAC387에 대한 면역반응에서 정상군은 모두 양성반응이 거의 나타나지 않았으나, 유발군의 HSP27과 HSP70에 대한 면역반응에서는 췌장염 유발 14일째까지 샘꽈리세포와 도관세포에서 양성반응이 증가되었으며, 21일째 HSP27에 대한 면역반응은 샘꽈리세포에서는 오히려 감소되고 도관과 혈관에서는 강한 양성반응을 나타낸 반면, HSP70의 면역반응은 샘꽈리세포와 도관 및 혈관에서도 거의 나타나지 않거나 매우 약한반응을 보였다. MAC387에 대한 면역반응에서 유발군은 시간이 경과되면서 면역반응을 나타내는 세포가 증가하였다. 반면, 야콘 추출물을 음용한 군의 HSP27과 HSP70에 대한 면역반응은 DBTC투여 후 14일째까지 약한반응을 보이다가 21일째 강한 양성반응을 나타내는 세포가 다소 증가되었으며, MAC387에 대한 반응은 7일째보다 14일째 오히려 감소한 경향을 보였으며 21일째 다시 증가되었으나 유발군에 비해 매우 낮은 정도였다. 이러한 결과로, 1% 야콘 추출물은 췌장염에 대한 염증진행을 억제 및 완화시키는 효과가 있음을 확인하였다. Yacon is used in folk medicines as a medicinal tea for hypoglycemia. In a recent study described herein, anti-oxidative, -bacterial, -fungal activities and cell protective functions of leaf extract of Smallanthus sonchifolius have been reported. In this study, to evaluate the effect on pancreatitis by using yacon extract (YE), we examined the efficacy of YE on DBTC (8 mg/kg)-induced pancreatitis in rats. After the treatment of DBTC on day 21, highly increased inflammatory cells, necrosis of acinar cells and connective tissues were observed. However, the inflammation was strongly inhibited in YE as compared to control group (CON). As a result of immunoreactions against HSP27, HSP70 and MAC387, weak or no possibilities of normal group (NOR) were observed in pancreas, whereas in immunoreactions against HSP27 and HSP70, strong expression of CON was observed in acinar and duct cells on day 14. However, the expression of HSP27 was decreased in acinar cells for CON treatment on day 21 as compared to 14 days observation, whereas strong expression of HSP27 was observed in duct cells and blood vessels on day 21. As regards the immunoreactions against HSP70, weak or no possibilities of CON were observed in duct cells and blood vessels as well as in acinar cells on day 21. In CON, time-dependent increase of MAC387 was observed. However, the expressions of HSP27 and HSP70 were weakly observed in YE as compared to CON on day 14 with strong positive reaction in acinar cells on day 21. Immunoreactions against MAC387 were decreased in YE on day 14 than day 7, which increased on day 21 as compared to day 14. These results suggested that yacon extract has inhibitory and mitigatory effects against DBTC-induced pancreatitis.