Some Properties of Partially Purified Alkaline Inorganic Pyrophosphatase from Mung-bean (Phaseolus radiatus) Seedling

박우철,남민희,Park, Woo-Churl,Nam, Min-Hee 한국토양비료학회 1982 한국토양비료학회지 Vol.15 No.4

발아초기 녹두의 부위별 alkaline inorganic PPase의 활성변화 및 잎 부위에서 부분 정제하여 얻은 효소를 이용하여 효소적 성질을 조사한 결과를 요약하면 다음과 같다. 1. 잎 부위가 타 부위보다 약 2~4배의 더 높은 활성을 나타냈으며 발아가 진행됨에 따라 잎과 뿌리 그리고 지엽에서는 효소 활성이 초기에 증가하다가 점차 감소하는 경향을 보인 반면, 상배축에서는 초기부터 계속 감소하였으며 하배축에서는 계속 증가하는 경향을 보였다. 2. 정제 과정동안 23.9%의 수율로 86배가 정제되었으며 전기 영동상의 Rm value는 0.35였으며 homogenenity는 아니었고, Km value는 0.89mM로 나타났다. 3. 이 효소는 $Mg^{2+}$에 대해 대단히 Specific하였으며 $Cu^{2+}$와 $Fe^{2+}$도 $Mg^{2+}$에 비해 각각 56%, 55%의 activating effect를 나타냈다. 그러나, $Cu^{2+}$, $Zn^{2+}$, $Mn^{2+}$, $Co^{2+}$와 $Ni^{2+}$은 이 효소에 대해 저해체로 작용하였다. 4. 이 효소는 pH 8-9와 $50^{\circ}C$에서 최대의 활성을 보였으며 열에 대해서도 상당히 안정하였다. Time course of alkaline inorganic PPase activity with different parts of mung-bean sprout and some properties of partially purified enzyme from mung-bean leaves were investigated. The enzyme activity in leaf, root and cotyledon showed a tendency to increase at an early stage and then decrease gradually as germination continued. However, the crude homogenate of epicotyl showed the continuous decline of the enzyme activity but that of hypocotyl showed the continuous increase. In particular, the enzyme activity of leaf fraction was about 2-4 times as high as those of other fractions. The specific activity of the leaf enzyme was increased 86-fold, with a 23.9% yield, upon purification procedures. The purified enzyme from leaves had the Rm value of 0.35 and was not homogenous when judged by disc gel electrophoresis. Using tetrasodium pyrophosphate as a substrate, the apparent Km value for the partially purified enzyme was 0.89 mM. The enzyme was highly specific for $Mg^{2+}$ $CU^{2+}$ and $Fe^{2+}$ was also showed the activating effect of 56% and 55% with $Mg^{2+}$, respectively. However, $Ca^{2+}$), $Zn^{2+}$, $Mn^{2+}$, $Co^{2+}$ and $Ni^{2+}$ acted as inhibitors for the enzyme. The pH optimum for the enzyme shifted from 9.0 to 8.0 as the concentration of $Mg^{2+}$ was increased. The enzyme from mun-bean leaf was the most active at $50^{\circ}C$ and considerably stable on heat.

정례적 결혼검진의 가족센터 적용에 관한 효과성 연구

박우철,Park, Woochul 대한가정학회 2022 Human Ecology Research(HER) Vol.60 No.1

The present study examined the effectiveness of repeated marriage checkups at intervals of six months in a Family Center in Seoul. To achieve this, the pilot study protocol (Park, 2019) was adapted, emphasizing strength-based, counseling-oriented approaches. Two couples and family therapists with master's degrees were trained and their practice was monitored to ensure program fidelity. The methods employed for the assessment of marriage checkup were an online survey, a video recording of couple problem-solving, and a clinical interview. A total of 14 couples participated in the 1st checkup, 10 of whom also completed the 2nd checkup. Couples with varying levels of marital satisfaction participated in the program. Descriptive statistics indicated gradual increases over 8-9 months in diverse indices such as marital satisfaction and relationship strength, while other indices fluctuated slightly but converged to higher levels over time. A non-parametric analysis appropriate for small samples demonstrated statistically significant increases over the 8-9 months in marital satisfaction among couples. The current study evidenced the effectiveness of repeated marriage checkups in the Family Center, which is the primary public institution for family policy and services in Korea. Repeated marriage checkups are therefore a promising way to enhance couples' relationship health and improve the family management system of the Family Center.

NCW대비 군 위성통신 구조 분석

박우철,차재룡,김재현,Park, Woo-Chul,Cha, Jae-Ryong,Kim, Jae-Hyun 한국위성정보통신학회 2009 한국위성정보통신학회논문지 Vol.4 No.1

본 미래 전쟁 양상은 정보화 시대의 도래와 함께 다른 분야와 마찬가지로 전쟁수행 여건에도 많은 변화를 가져왔다. 이에 따라 정보우위와 정보전이 중요시되고 네트워크 중심전(Network Centric Warfare) 등 새로운 전쟁수행 개념이 등장하게 되었다. 군 위성통신체계는 미래 NCW 전장환경에서 전장정보의 실시간 전송이 가능하며, 전장의 광역화, 자동화, 신속화 개념을 지원하고, 광역성, 이동성, 생존성 및 융통성이 보장된 전천후 핵심전략 통신체계이다. 이러한 군 위성통신시스템은 실시간 전장정보전송을 위한 광역 대용량 링크, 생존성, 이동간 통신을 제공하는 체계로 분류된다. 본 논문에서는 이러한 기능을 통합하여 지원하는 미군의 차세대 TSAT체계의 주요 운용개념과 핵심기술을 분석하였다. 분석 결과를 바탕으로 NCW 대비한 차세대 군 위성통신체계 상위 구조는 재밍이나 간섭 등의 채널환경에 최적으로 적응하여 서비스가 가능한 전송율, 항재밍 능력, 망 제어 및 망 관리 능력을 갖추어야 하며 고속 이동 차량, 초소형단말, 초고속 무인기 등의 다양한 플랫폼을 지원해야 하는 등 향상방안을 제안하고, 또한 이러한 체계 구조를 위한 소요기술로 적응형 다중안테나, 레이저 링크, 차세대 항재밍 waveform을 제안한다 As the information age comes out, the aspect of future war brings about the many changes in terms of war-fighting environment. Accordingly, information superiority and intelligence-centric warfare have been important and new war-fighting concept such as NCW(network centric warfare) have been turned up. This paper proposed all-weather core-strategy communications systems guaranteeing not only the real-time transmission of the information collected in a battlefield and expansion, automation, and rapidity of a battlefield but also broadband, mobility, survivability, and flexibility. The proposed military satellite communications system is classified into wideband mass capacity link, survivability, and the system supporting OTM(on the move) communication for the real-time transmission of battlefield information. This paper analyzed the essential operation concepts and core schemes of the U.S. Army's next generation system, TSAT(Transformational Satellite Communication System). Base on the analysis results, this paper proposed that the architecture of next generation military satellite communications systems for NCW have to provide the data rate, anti-jamming capability, network control and management capability which are optimally adaptable for the wireless channel environments such as jamming and interference and to support the variety of platforms like high-speed mobile vehicles, micromini devices, super-high speed unmanned aerial vehicles. Finally, this paper also proposed that next generation military satellite communications systems need the technologies such as the adaptable multi-antenna, laser link, and next-generation anti-jamming waveform.

전기자동차 및 플러그인 하이브리드 전기자동차 용 전력모듈형 차량 탑재형 완속충전기

박우철(Woo-chol Park),유석정(Seog-jeong Yoo),전준영(Joon-young Jeon),황선민(Sun-min Hwang),문진동(Jin-dong Moon) 대한전기학회 2013 대한전기학회 학술대회 논문집 Vol.2013 No.10

부부 결혼검진: 부부의 관계건강을 위한 새로운 접근

박우철 ( Park Woochul ) 한국가족관계학회 2019 한국가족관계학회지 Vol.24 No.2

Objectives: It is well-established in the public health literature that marriage relationship health is a powerful predictor of physical and mental health. Inspired by the annual health checkup model in public health, marriage relationship health checkup was developed by Cordova et al.(2001). The present study applied the marriage checkup at one of the Healthy Family and Multicultural Family Support Centers which are nationwide community-based institutions delivering Korean Healthy Family Policy to the community. Method: Sixteen married couples participated in the marriage checkup. They attended the assessment session where survey, observation of problem-solving discussion, and short interview were conducted. Then, the couples participated in the feedback session two weeks later which provided results of the checkup and some recommendations for the relationship. Results: It was found that the marriage checkup couples differentiated from the couples who sought couple therapy in their level of marital adjustment and other characteristics, such as, mental health. The marriage checkup significantly improved the couples’ understanding of relationship, knowing how to improve relationship, and self-efficacy. Participants reported high level of satisfaction with the marriage checkup at the posttest. Conclusions: This study shows that the marriage checkup is a promising program for attracting new families to the center and improving their relationship health. To fit the context of Korean Healthy Family Policy, a new operation system, ‘Family Relationship Health Management System’ in which the marriage checkup plays a pivotal role of attracting and assessing couples/families is proposed.

후기 아동기 및 초기 청소년의 신체상불만족과 자존감: 종단적 패널 연구

박우철 ( Woochul Park ) 대한가정학회 2020 Human Ecology Research(HER) Vol.58 No.2

Longitudinal studies have shown mixed findings on the association between body image dissatisfaction and self-esteem among adolescents. This study examined the longitudinal, reciprocal influences between body image dissatisfaction and self-esteem among Korean pre- and early adolescents by using nationally representative longitudinal panel data from the Korean Youth Panel Study (KYPS). Analyses based on 2,230 participants indicated that girls’ body image dissatisfaction was significantly higher than boys’. The trajectory of body image dissatisfaction increased sharply during pre- and early adolescence, but stabilized afterwards for both boys and girls. Self-esteem deteriorated rapidly initially, but began to recover as adolescent girls and boys matured. Structural equation modeling showed that reciprocal influences between body image dissatisfaction and self-esteem were detected for boys. Body image dissatisfaction among girls predicted self-esteem during the entire period, while the opposite direction from self-esteem to body image dissatisfaction emerged two years later. The present study found that, during pre- and early adolescence, body image dissatisfaction and self-esteem develop in a circular fashion by forming a vicious cycle. Implications for the development of intervention and prevention programs are discussed in consideration of age and gender.

포괄적 부부상호작용 코딩시스템(MICS-G) 국내 타당화 연구

박우철 ( Woochul Park ) 대한가정학회 2019 Human Ecology Research(HER) Vol.57 No.1

Few studies have utilized observational methods in the field of couple research even though using self-report questionnaires is prone to the subjective biases of the reporter. This study validates the Marital Interaction Coding System-Global (MICS-G), a global version of the well-established microanalytic observational coding system, Marital Interaction Coding System (MICS). Participants in the study consisted of 30 married couples with varied levels of marital adjustment who visited one of the Healthy Family and Multicultural Family Support Centers in Seoul, either for couple therapy or the “Marriage Checkup”program. Ten-minute problem-solving discussions were rated by two undergraduate student raters who were trained for 10 hours. Interobserver agreement based on percentage agreement and intraclass correlation coefficients showed a high level of agreement between raters in establishing interrater reliability. Convergent validity was established by: correlations among marital adjustment, psychological aggression, mental health, and MICS-G categories of conflicts, validation, invalidation, facilitation, and withdrawal. MICS-G categories also were successful in discriminating between distressed and nondistressed couples, which provides evidence of discriminant validity for MICS-G. This study showed that MICS-G is a promising method for researchers to observe couple interactions in a more cost-effective way. Methodological issues and practical applications are also discussed.

재생골재를 사용한 고강도 철근콘크리트 보의 전단성능에 관한 실험적 연구

박우철(Park, Woo Chul),이경희(Lee, Kyoung Hee),박완신(Park, Wan Shin),윤현도(Yun, Hyun Do),정수영(Chung, Soo Young) 한국콘크리트학회 2001 한국콘크리트학회 학술대회 논문집 Vol.2001 No.11

Lemna-Blue green algae에 依한 窒素固定에 関한 硏究

朴愚喆(W. C. Park),谷田澤 道彦(M. H. Yatazawa) 한국토양비료학회 1979 한국토양비료학회지 Vol.12 No.1

대시포트형 MR유체 마운트의 설계 인자에 대한 실험적 고찰

박우철(Park Woo-Chul),김일겸(Kim, Il-Gyoum),이현창(Lee Hyun-Chang) 한국산학기술학회 2009 한국산학기술학회논문지 Vol.10 No.12

본 연구에서는 MR유체를 특징으로 하는 대시포트형 마운트를 제안하고, 자극 형상 및 구조의 변화가 MR 유체 마운트의 감쇠력에 미치는 영향에 대하여 실험적으로 고찰하였다. 자극의 유효길이와 코어 중심부의 구조를 달 리하여 MR 유체 마운트를 제작하였다. MR유체 마운트에 자기장을 형성하기 위하여 공급하는 전류의 세기를 변화시 켰을 때의 감쇠력 변화와 전류를 공급하지 않은 경우의 감쇠력 변화를 측정하였다. 또한, 가진 변위는 일정하게 유지 한 상태에서 MR엔진마운트에 가해지는 가진 주파수를 변화시켜 감쇠력의 변화를 측정하였다. MR유체마운트로부터 전달되는 힘은 가진 주파수가 증가함에 따라 감소하는 경향을 나타냈으며, 공급하는 전류의 세기가 증가함에 따라 전 달되는 힘은 증가하여 나타났다. 자기장이 형성되는 MR마운트에 유효길이 변화에 대해서는 전달력의 변화가 나타나지 않았다. This research proposed a dashpot type mount design using MR fluids, and investigated experimentally the influence of the design parameters of the dashpot MR fluid mount, which affect the damping forces of the dashpot MR fluid mount. In order to observe the influence, the dashpot MR fluid mount which have the different effective length and the core structure is manufactured. The variations of the resistance forces according to different effective lengths of the magnetic pole of MR fluid mount, along which magnetic field is defined, was investigated. It was founded that the resistance forces from the MR mount decreased with increased input frequencies, while increased with increased applied electric current intensities. Nevertheless, there is no appreciable change in the resistance forces with respect to the effective length variations of the magnetic pole of MR fluid mount.