백마강수역(白馬江水域)에 있어서 오염세균(汚染細菌)에 대(對)한 조사연구(調査硏究)

박양원,김형석,Park, Yang-Won,Kim, Hyung-Suk 대한예방의학회 1974 Journal of Preventive Medicine and Public Health Vol.7 No.1

The purpose of this study was to survey bacteria which was known as a fecal bacteria living in the human and animal gastro and intestinal tract. The colony density of the fecal coliform and fecal streptococci was 280 and 130 in 100ml sample amounts reapectively. Staphylococcus aureus and Pseudomonas aeruginosa which is usually exist in swimming pool and was known as indicator of the pool water contamination was detected.

Induction of Escherichia coli $oh^8$Gua Endonuclease by Some Chemicals in the Wild Type and mutM Mutant Strains

박양원,강경화,김훈식,정명희,최경희,Park, Yang-Won,Gang, Gyeong-Hwa,Kim, Hun-Sik,Jeong, Myeong-Hui,Choe, Gyeong-Hui The Korean Society for Integrative Biology 1997 Korean journal of biological sciences Vol.1 No.3

The effects of nalidixic acid, mitomycin C, and cadmium chloride $(CdCI_2)$ on the activity of 8-hydroxyguanine $(oh^8Gua)$ endonuclease, a DNA repair enzyme for oxidatively modified guanine, $(oh^8Gua$ were studied. Nalidixic acid and mitomycin C, typical inducers of the S0S DNA repair response in E. coli, showed different effects. Nalidixic acid raised the activity of this enzyme, but mitomycin C did not show such an effect. Cadmium chloride also induced the enzyme activity, These results show that the expression of $oh^8$ Gua endonuclease is regulated by multiple factors and can be induced under stressful conditions. In an attempt to demonstrate the importance of this enzyme in defense against DNA damage and mutagenesis, we also characterized mutM mutant for its oh8 Gua endonuclease activity. The mutM mutant showed no detectable $oh^8$ Gua endonuclease activity, unlike its wild type showing high activity. In addition, paraquat, a superoxide producing compound, failed to elevate $oh^8Gua$ endonuclease activity in this mutant. These results suggest that the mutM gene is identical to the $oh^8Gua$ endonuclease gene of E. coli. Taken together with previous reports, these results suggest that $oh^8Gua$ endonuclease plays a crucial role in the protection of aerobically growing organisms from threats of oxidative DNA damage and mutation.

대두 단백질의 특성과 그 이용

박양원(Yang-Won Park) 한국식품영양과학회 1993 한국식품영양과학회지 Vol.22 No.5

대두의 조성은 다른 두류나 곡류와는 달리 35~40%의 단백질, 15~20%의 유지 및 20~25%의 당을 함유하고 있다. 예로부터 대두는 두부, 간장, 된장과 같은 전통 식품의 원료로써 넓게 쓰여져왔다. 초원심분리에 의한 대두 단백의 성분은 침강정수에 의해 2, 7, 11및 15S의 4가지의 주요한 성분으로 나타난다. 저장단백질중 중요한 2성분인 7S 및 11S globulin은 많은 연구자들에 의해 분리되고, 특성이 알려졌다. 미생물 효소에 의해 제조된 응고물은 금속이온 및 산처리에 의해 만들어진 것보다 훨씬 조밀한 구조를 나타내었다. 효소작용으로 얻어진 curd는 식품재료로써 개발되어지고, 식품가공분야에서도 폭넓게 이용될 것으로 기대된다. Soybean composition, which is different from those of other beans and grains, gives from 35 to 40 percent protein, 15 to 20 percent oil, and 20 to 25 percent sugar. Soybean has been extensively used as the raw material for traditional foods such as bean curd, soy sauce, soy paste and so on, since ancient times in Korea. Ultracentrifugal components of the soybean proteins represent four major peaks with sedimentation constants of about 2, 7, 11 and 15S. The two major reserve protein of soybean, 7S and 11S globulins, have been isolated and characterized by many workers. The curd made with microbial enzyme exhibited minuter structure than those of the metal ion- and acid-treatment. Thus, the curd obtained by enzymatic operation serves as a material for further development of food items, and the procedure may be widely applicable in food processing.

파일럿 규모 연소기에서 밀짚 펠렛 혼소 시 첨가제에 따른 회 점착의 특성 연구

박유미(Yumi Park),임호(Ho Lim),채태영(Taeyoung Chae),이재욱(Jaewook Lee),고대홍(Dae-hong Ko),양원(Won Yang) 한국열환경공학회 2019 한국열환경공학회 학술대회지 Vol.2019 No.춘계

최근 국내 미활용 바이오매스에 대한 관심이 증가하고 있고 미분탄 보일러에 적용 가능성을 타진하고 있다. 그러나 미분탄 보일러에 바이오매스 혼소 적용 시 회 발생은 중요한 문제로 이를 감소시키는 것은 보일러의 효율적인 운전을 위해 반드시 필요하다. 따라서 본 연구의 목적은 회 점착에 영향을 미칠 수 있는 다양한 첨가제에 따라 석탄, 바이오매스 혼소 시 발생하는 회 점착 특성, 특히 증저감 특성을 규명하는 것이다. 본 연구에서 사용된 바이오매스는 초본계로 밀짚 펠렛(straw pellet)이다. 첨가제로는 카올린(Al2Si2O5(OH)4), 황산암모늄((NH4)2SO4), 인산수소칼슘(CaHPO4), 산화 알루미늄 (Al2O3), 산화 칼슘(CaO)을 선택하여 비교하였다. 연료의 공업분석, 원소분석 및 ICP 등의 기초 분석을 수행하여 연료 특성을 확인하였다. 분석 결과를 바탕으로 80kWth 설비를 사용하여 바이오매스와 석탄 혼소 시 회 점착 거동을 조사하였다. 회 점착 생성 정도는 실시간 무게 측정이 가능한 측정 장비(on-line deposit measuring system)를 활용하여 회 점착량을 측정하였으며, 첨가제에 따른 회 점착 무게를 비교하였다. 그리고 다양한 첨가제 사용에 따른 메커니즘을 분석하기 위해 회 점착을 SEM/EDS 분석을 수행하였다. 결과적으로 동일한 연료가 사용되더라도 첨가제의 종류에 따라 회 점착 형성 메커니즘의 차이로 인해 회 점착을 촉진 또는 억제하는 다양한 결과가 발생함을 확인하였다.

광주지역 고등학생의 수면부족에 따른 간식 및 군것질 섭취에 대한 실태조사

박인자 ( In Ja Park ),박양원 ( Yang Won Park ) 한국식생활문화학회 2009 韓國食生活文化學會誌 Vol.24 No.3

The purpose of this study was to determine conditions of dining behaviors, sleep patterns, rates of obesity and intake of snacks and self-purchased snacks deriving from the lack of sleep in academic high school students. The anthropometric characteristics, height and weight (p<0.05), and the distribution of obesity index (p<0.001) showed significant differences between males and females. Dining scores for both male and female students decreased as the frequency of day time napping increased. Although, there was no significant difference in obesity-related "lack of sleep" between males and females, the rate of "being overweight" was slightly higher in female students who indicated that they experienced a "lack of sleep" than female students that indicated "no lack of sleep". While, whether intake of snacks and self-purchased snacks or not, day time napping frequency affected not to male students. However, the frequency of day time napping among females who ate snacks one or more times a day was higher (p<0.05), and a greater frequency of eating self-purchased snacks correlated with a greater frequency of day napping (p<0.01). Consequently, lack of sleep was identified as one of the factors increasing the frequency of intake of snacks and self-purchased snacks in females. Thus, this study suggests that dining behavior guidance should be given along with systematic and continual nutrition education so that the intake of snacks and self-purchased snacks can be moderated to optimize the physical and mental growth and development of adolescents.

서울근교(近郊)의 산악수(山岳水) 및 약수(藥水)에 관(關)한 연구(硏究)

박양원,이병갑,김형석,박순영,Park, Yang-Won,Lee, Pyong-Kap,Kim, Hyung-Suk,Park, Soon-Young 대한예방의학회 1972 Journal of Preventive Medicine and Public Health Vol.5 No.1

Mountain Wells are very important water source for the most of the climbers in Seoul area. But the facilities around the wells are not modernized to prevent from contamination of microorganism and the other pollutants. We focussed our intention on the detection of some harmful chemical ingredients and contaminants. A total of 58 wells were examined according to the Standard Methods(APHA) and the following results were obtained: 1) Among the examined 58 wells, only 10 wells were adequate to the drinking water standard, 2) 7.9mg of liberated $CO_2$ was found in the Tchun-Ho-Dong Mineral Well, 3) Chemically harmful ingredients were not found, 4) The Mountain Wells were contaminated by Coliform groups as many as 18 times than rural wells.

일부사립대학교(一部私立大學校)의 학생(學生)의 성장(成長).발육(發育) 및 건강상태(健康狀態)에 관(關)한 조사연구(調査硏究)

박양원,이병갑,박순영,김형석,김재명,Park, Yang-Won,Lee, Pyong-Kap,Park, Soon-Young,Kim, Hyong-Suk,Kim, Jae-Myung 대한예방의학회 1972 Journal of Preventive Medicine and Public Health Vol.5 No.1

For the purpose of ascertaining the status of physical growth and health status of the University Students, an intensive survey was conducted by the authors towards a total of 1,250 (Male 792, Female 458) who passed the written entrance examination at the Kyung Hee University in 1972. The items included the measurements on physical growth, various physical and nutritional indices, status of visual distourbance, dental status and tuberculosis. The findings and results cad be summarized as follows; 1. Physical Growth and Develspment. i) The average of body height by anthropometric was $169.39{\pm}5.05cm$ in male and $157.45{\pm}4.43cm$ in female. ii) The averages of body weight by sex were $59.42{\pm}6.47Kg\;and\;51.81{\pm}5.21Kg$, respectively. iii) The averages of chest-girth were $87.18{\pm}5.30cm\;and\;80.51{\pm}4.53cm$. iv) The averages of sitting-height were $92.21{\pm}2.79cm\;and\;86.28{\pm}2.57cm$. In all cases the male measurements were higher than female. 2. Various ludices about Physical Growth and Development; i) Relative body weights by sex were 35.08 and 32.91, relative chestgirth 51.47 and 51.13, and relative sitting-height 54.43, 54.79, respectively. ii) Rohrer index was 1.222 in males and 1.327 in females, Kaup index 2.071 and 2.089, Verveck index 86.54 and 84.04, and Pelidisi index 91.15 and 93.08, respectively 3. Status of visual distourbance The vision of the students under 0.8 with Landolt's testing chart was 45.4% in left eye and 46.6% in right eye(male: 44.4% and 45.7%, female: 47.2% and 48.0%), while under 0.6 in both visions was 41.0% and 40.5% (male: 40.8% and 40.7%, female: 41.5% and 40.2%), respectively. 4. Dental Status Out of total 19.4% (male 19.2%, female 19.6%) had gingivitis, the female incidence rate of gingivitis was higher than male. Average number of teeth in each subject was 29.6 teeth: male had 29.9 teeth and female had 29.1 teeth. The caries rate was 78.9%(male 75.6%, female 84.5%), the female caries rate was higher than male. Total filing per subject was 35.6% (male 34.0%, female 37.1%), female incidence was higher shan male. The average number of i) Caries per tooth rate was 8.5% in male and 9.2% in female, ii) Missing per tooth rate was 0.3% and 0.2%, iii) Filling per tooth rate was 3.9% and 4.2%. in all cases, the female incidences were higher than the male. The average number of D.M.F. was 3.8 teeth in male and 4.0 teeth in female, female was higher than male. The average rate of D.M.F. was 12.7% in male and 13.7% in female, female was higher than male. 5. Pulmonary infiltration Among the total, 53 students were diagnosed as pulmonary infiltration (tuberculosis), of whom 51 were minimal cases, one were moderately advanced case and one were far advanced case.

일부도시지역(一部都市地域)(회기동(回基洞))의 출생(出生), 사망(死亡)에 관(關)한 실태(實態) 조사연구(調査硏究)

박양원,이병갑,박순영,고순자,Park, Yang-Won,Lee, Pyong-Kap,Park, Soon-Young,Koh, Soon-Ja 대한예방의학회 1971 Journal of Preventive Medicine and Public Health Vol.4 No.1

A survey was couducted by the staff of the Dept. of Preventive Medicine, Kyung Hee University, School of Medicine, from April through May 1971, on such events as delivery, death, abortion and family planning. The survey directed to a total population 6,552 (Male 3,133; Female 3,419) residing in this area (1,262 households) led us to the following findings: 1) Two year averages of crude birth rate, crude death rate and natural increase rates were 24.9, 5.0 and 19.9 respectively. 2) Infant death rates for the years 1969 and 1970 were 13.2 and 5.8 respectively, mean for the two year period being 9.3. 3) Hospital deliveries rated 68.5 per cent, Home deliveries 28.4 per cent and deliveries at midwives' offices 2.7 per cent. 4) Deliveries other than hospital and midwives' office deliveries were found to be attended more often by mothers. 5) About 41.4 per cent of all pregnant women during last two years received prenatal care once or more. 6) The induced abortion rate was 6.7 per cent in 1969 and 7.5 per cent in 1970. 7) The spontaneous abortion rate was 1.1 per cent in 1969 and 1.4 per cent in 1970. 8) Hypertension was the most frequent cause of adult death(21.6%). 9) The rate of current practice in family planning was 43.3 percent of all women.

파일럿 규모의 연소에서 밀짚 혼소시 첨가제에 따른 회점착에 관한 연구

박유미(Yumi Park),임호(Ho Lim),이재욱(Jaewook Lee),채태영(Taeyoung Chae),고대홍(Dae hong Ko),양원(Won Yang) 한국연소학회 2019 KOSCOSYMPOSIUM논문집 Vol.2019 No.5

Bacillus sp. IJ - 3가 생산하는 대두단백응고효소의 최적생산조건 및 7S Globulin에 대한 효소적 작용에 관한 연구

박양원(Yang-Won Park),김영전(Young-Jeon Kim) 한국식품영양과학회 1996 한국식품영양과학회지 Vol.25 No.5

Bacillus sp. IJ-3 균주는 균체외 효소로 대두단백응고효소를 생산하고, 이 박테리아의 최적 효소생산에 대한 배지조성 및 배양조건을 검토하였다. 최대의 대두단백 응고효소를 생산하는 배지조성은 soymilk 20%(w/v), glucose 2.0%, yeast extract 5.0%, peptone 4.0% 및 potassium phosphate, monobasic 1.0%였다. 초기 pH는 6.0이었고, 35℃ 배양에서 최대 효소활성을 보였다. 조정된 배지 조성에서 72시간 배양에 최대 효소활성을 나타내었으며, 기존배지 활성의 약 5.3배의 효소활성의 증가를 보였다. Bacillus sp. IJ-3 균주효소는 7S globulin에 대해 특이성을 갖고 있는 것으로 사료된다. A bacterial strain, designated as Bacillus sp. IJ-3 strain, was shown to produce the extracellular soy protein coagulating enzyme and culture conditions for the production of enzyme by this microbial strain was investigated. The culture medium giving a maximum soy protein coagulating activity was consist of 20%(w/v) soymilk, 2.0%(w/v) glucose, 4.0%(w/v) yeast extract, 5.0%(w/v) polypeptone and 1.0%(w/v) potassium phosphate, monobasic. Initial pH was optimal at 6.0 and the enzyme activity in the culture usually reached a maximal level of fermentation at 35℃. After the culture medium adjustment where required, enzyme activity was reached maximum at 72 hour of cultivation but this enzyme activity was reduced quickly. It can be assumed that Bacillus sp. IJ -3 strain enzyme has a specificity toward the 75 globulin.