Enhanced Cellular Responses of Vascular Endothelial Cells on Poly-γ-Glutamic Acid/PU Composite Film Treated with Microwave-Induced Plasma at Atmospheric Pressure

박봉주,권병주,강재경,이미희,한인호,김정구,박종철 한국고분자학회 2011 Macromolecular Research Vol.19 No.6

Poly-γ-glutamic acid (γ-PGA), which is produced by microbial fermentation, is a biodegradable, hydrophilic and non-toxic biomaterial. γ-PGA has many carboxyl groups that makes it a polyanionic biopolymer with swelling ability, biocompatibility and anticoagulant activity. On the other hand, few studies have examined the effect of γ-PGA on the cellular activity of human umbilical vein endothelial cells (HUVECs). The present study evaluated the effects of γ-PGA and fabricated γ-PGA/PU composite films on the attachment and proliferation of HUVECs after treatment with microwave plasma at atmospheric pressure. The results confirmed that γ-PGA is capable of increasing the proliferation and differentiation of HUVECs to form capillary tubes with enhanced alignment and organization. Moreover, the microwave plasma modified γ-PGA/PU composite film was more hydrophilic and the surface roughness was enhanced. In addition, the attachment and proliferation of the HUVECs were increased by the plasma treatment. These results suggest that γ-PGA and surface modified γ-PGA/PU composite can be applied as bioactive and biocompatible materials in vascular tissue engineering but further testing of γ-PGA and γ-PGA/PU composite films will be needed to confirm the potential effects for use in vascular applications.

天保 改革의 展開 과정과 性格

朴俸住 서울대학교 동양사학과 1995 서울大 東洋史學科 論集 Vol.19 No.-

In Vitro Antifungal Activity of Epigallocatechin 3-O-Gallate against Clinical Isolates of Dermatophytes

박봉주,박종철,Hideaki Taguchi,Katsuhiko Kamei,Tetsuhiro Matsuzawa,현성휴 연세대학교의과대학 2011 Yonsei medical journal Vol.52 No.3

Previously, we reported that epigallocatechin 3-O-gallate (EGCg) has growth-inhibitory effect on clinical isolates of Candida species. In this study, we investigated the antifungal activity of EGCg and antifungal agents against thirty-five of dermatophytes clinically isolated by the international guidelines (M38-A2). All isolates exhibited good susceptibility to EGCg (MIC_50, 2-4 μg/mL, MIC_90, 4-8 μg/mL, and geometric mean (GM) MICs, 3.36-4 μg/mL) than those of fluconazole (MIC_50, 2-16 μg/mL, MIC_90, 4-32 μg/mL, and GM MICs, 3.45-25.8 μg/mL) and flucytosin (MIC_50, MIC_90, and GM MICs, >64 μg/mL), although they were less susceptible to other antifungal agents, such as amphotericin B, itraconazole, and miconazole. These activities of EGCg were approximately 4-fold higher than those of fluconazole, and were 4 to 16-fold higher than flucytosin. This result indicates that EGCg can inhibit pathogenic dermatophyte species. Therefore, we suggest that EGCg may be effectively used solely as a possible agent or combined with other antifungal agents for antifungal therapy in dermatophytosis.

先秦 楚文化의 擴散 과정과 多元的 構造

朴俸住 동양사학회 1999 동양사학회 학술대회 발표논문집 Vol.1999 No.-

〈시〉 사모곡

박봉주 샘터사 1996 월간 샘터 Vol.27 No.10

갈 곳 없는 이들의 보금자리

박봉주 새가정사 1993 새가정 Vol.- No.-

Surgical Treatment of Undisplaced Femur Neck Fractures in Dementia Patients Using Proximal Femoral Nail Antirotation

박봉주,조홍만,민웅배 대한고관절학회 2015 Hip and Pelvis Vol.27 No.3

Purpose: People with dementia have poor mobility and discharge outcomes following hip fractures. The purpose of this study was to evaluate the clinical and radiological results of internal fixation of undisplaced femur neck fractures (Garden types 1 and 2) by proximal femoral nail antirotation (PFNA) in dementia patients. Materials and Methods: We studied retrospectively 19 patients with undisplaced femur neck fracture. All patients were over 70 years of age, walked independently with a cane or crutches and suffered moderate-tosevere dementia. Patients were treated with PFNA and followed-up for more than 2 years. Revision, loss of fixation, complications, and walking ability outcomes were measured. Results: In walking-ability evaluation, patients showed an average decrease of just 0.2 points at the final followup. Walking ability was evaluated from before injury to 4 weeks after surgery and decreased by less than 0.5 points. Radiological bone union was achieved in 17 cases; the average time to bone union was 4.14 months (range, 2.5-7 months). Complications included non-union in two cases and femoral head avascular necrosis in one case of non-union. Conclusion: We found that for patients with osteoporotic bone tissues in their femoral heads or patients (e.g.,those suffering dementia) for whom cooperating with medical workers for postoperative walking control or rehabilitation exercises is difficult, implanting a mechanically stable spiral blade for fixation of femoral neck fractures could facilitate walking after surgery.

包山楚簡에 반영된 楚의 司法 제도

朴俸住 서울대학교 동양사학과 1997 서울大 東洋史學科 論集 Vol.21 No.-

말초 신경 감압술이 당뇨발에 미치는 효과

박봉주,김주오,양경호,최승준,Park, Bong-Ju,Kim, Ju-O,Yang, Gyoung-Ho,Choi, Soeng-Jun 대한족부족관절학회 2004 대한족부족관절학회지 Vol.8 No.1

Purpose: We evaluated the effect of nerve decompression for restoration of plantar sweating and sensation in diabetic neuropathic feet, and we selected diabetic neuropathic patients with the possibility of overlying entrapmental neuropathy. Materials and Methods: From June 2002 to May 2003, we have investigated and follow-up examed 10 patients with diabetic neuropathic feet, with decreased sensation in their lower limb, who underwent peripheral nerve decompression. The surgical procedure was multiple neurolysis of the common peroneal nerve, posterior tibial nerve and its three branches of one limb. We compared the operated limb with the opposite, unoperated limb. We performed history taking, physical examination, sweat secretion test, touch sensory test using Semmes-Weinstein monofilaments and electrodiagnostic study, pre-operatively and post-operatively. Results: On 6 months after the operation, the post-operative tests showed that there were noticeable improvements to sensation, statistically (P<0.05), but there was no change in the sweat secretion test. According to the Cseuz criteria, 7 patients out of the 10 patients who received the multiple neurolysis showed excellent or good results. Conclusion: We observed that the peripheral nerve neurolysis could be benefit for improving sensation and alleviating pain of the diabetic neuropathic feet with nerve entrapmental symptoms, but there was no change in the sweat secretion on short-term follow-up. To identify whether the effect will be continued or not, additional follow-up will be required.

잔디밤나방에 대한 난지형 잔디와 한지형 잔디의 내충성 평가

박봉주 한국조경학회 2003 韓國造景學會誌 Vol.31 No.4

The need for insect and mite resistant turfgrass cultivars arose because of problems associated with pesticide use. Representative cultivars and genotypes of 18 warm-season turfgrass [Zoysia japonica Steud., Z. japonica${\times}$Z. metrella hybrids, Z. japonica${\times}$Z. tenuifotia hybrids, Z. matrella (L.) Merr., Cynodon dactylon (L.) Pers., C. dactylon${\times}$C. transvallensis hybrids, Paspalum notatum Flugge., P. vaginatum Swartz., Stenotaphrum secundatum (Walt.) Kuntze, Eremochloa ophiuroides (Munro.) and Buchloe dactyloides (Nutt.) Engelm.] and 20 cool-season turfgrasses [Poa pratensis L., Festuca arundinacea Schreb., F. rubra L., F. rubra var. commutata Gaud., F. ovina var. duriuscula L. Koch. Agrostis tenuis Sibth., A. palustris Huds., and Latium perenne L.] were evaluated for host resistance to feeding by the Spodoptera depravata (Butler) in the laboratory. Two experiments were set up in the laboratory using 8.5cm diameter${\times}$4.0cm deep plastic petri dishes as larvae feeding chambers. In experiment 1, one neonate larvae were place on the grass in each dish and the dishes were arranged with 5 replicates each within an environmental chamber maintained at $25^{\circ}C$ and 15h light: 9h dark Larval survival and larval weights at 7d and 14d, pupal weights, and days to pupation were compared among turfgrasses. In Experiment 2, 4cm sections of all grasses were oriented equidistant from each other in a pattern resembling the spokes of a wheel. Five one neonate larvae were introduced to the center of each dish. Dishes were immediately placed in an environmental chamber held at $25^{\circ}C$, 15h light: 9h dark Larvae were allowed to feed for 24h. Damage was rated from 0(no damage) to 9(completely consumed) were made for eachturfgrass. Resistance as antibiosis (high mortality, slowed growth, and least preference) was identified in Z. japonica${\times}$Z. tenuifolia hybirids ‘Emerald’, Z. japonica${\times}$Z. metrella hybirds ‘Miyako’ and Eremochloa ophiuroides (Munro.). Cool-season turfgrasses tested were susceptible to feeding by Spodoptera depravata (Butler).