조피볼락, sebastes schlegeli의 Glutathione 포합효소에 미치는 유기주석의 영향

민은영,지정훈,김성길,황운기,강주찬 한국어병학회 2002 한국어병학회지 Vol.15 No.3

Thermal Effects on Bioaccumulation and Antioxidant Enzyme Response in the Liver and Gills of Tilapia Oreochromis niloticus Exposed to Arsenic

민은영,정지원,강주찬 한국수산과학회 2015 Fisheries and Aquatic Sciences Vol.18 No.2

The effects of waterborne arsenic (As) exposure on bioaccumulation and antioxidant defenses were examined in the liver and gills of tilapia, Oreochromis niloticus, under thermal stress. Tilapia were exposed to different As concentrations (0, 200, and 400 μg L-1) at three water temperatures (20, 25, and 30°C) for 20 days. After As exposure, higher levels of As accumulation were observed in the gills compared with the liver in elevated water temperatures. In terms of the antioxidant response, glutathione (GSH) levels and the activities of glutathione reductase (GR), glutathione peroxidase (GPx), and glutathione S-transferase (GST) significantly decreased in the liver and gills of tilapia exposed to As for 20 days, regardless of the As concentration (200 and 400 μg L-1) or water temperature. These enzyme activities exhibited greater fluctuations in the liver and gills of tilapia after As exposure in water warmer than 20°C. The present findings suggest that the simultaneous stress of temperature change and As exposure can accelerate As accumulation and alter the antioxidant enzymes activities of tilapia.

Aroclor 1254 May Induce Common DNA Effects in Developing Paralichthys olivaceus Embryos and Larvae

민은영,강주찬 한국수산과학회 2014 Fisheries and Aquatic Sciences Vol.17 No.4

Polychlorinated biphenyls (PCBs) are persistent pollutants in aquatic environments, often causing the decline or disappearance of wild populations. In this study, we used a random amplified polymorphic DNA (RAPD) assay to evaluate the effects on the genomic DNA of olive flounder embryo and larval stages of exposure to Aroclor 1254 at concentrations of 1, 5, 10, 20, and 40 μg/L. We compared RAPD fingerprints of exposed and non-exposed samples. Polymorphisms were revealed as the presence and/or absence of DNA fragments between the two samples. A dose-dependent increase in the number of polymorphic bands was observed with Aroclor 1254 treatment. Also, RAPD profiles of animals exposed to Aroclor 1254 exhibited an increase in the frequency values (FV) compared to the control. A phenogram constructed using neighbor-joining method indicated that genomic template stability in developing embryo and larval stages was significantly affected at ≥ 5 μg/L. This study suggested that DNA polymorphisms detected by RAPD analysis could be used as an investigative tool for environmental toxicology and as a useful biomarker in early life stages for the detection of potential genotoxicants.

Combined Effects of Copper and Temperature on Antioxidant Enzymes in the Black Rockfish Sebastes schlegeli

민은영,백수경,강주찬 한국수산과학회 2014 Fisheries and Aquatic Sciences Vol.17 No.3

Copper has been widely used to control algae and pathogens in fish culture ponds. However, its toxic effects on fish depend not only on its concentration in the water but also on the water quality. A laboratory experiment was conducted to assess copper toxicity in the black rockfish Sebastes schlegeli using a panel of antioxidant enzymes, including glutathione (GSH), glutathione S-transferase (GST), glutathione peroxidase (GPx), glutathione reductase (GR) and superoxide dismutase (SOD), at different levels of copper at three water temperatures (WT, 18, 23, 28°C) for 4 days. After exposure to two copper concentrations (100 and 200 μg/L), GSH levels and GST activities increased significantly, depending on WT (P < 0.05) in the liver, gill, and kidney of the black rockfish. GPx and SOD activities decreased significantly with both increasing WT and copper treatment in the organs of black rockfish (P < 0.05). These changes can be seen as initial responses to temperature stress and as a sustained response to copper exposure. This also indicates that GSH and related enzymes activities were sensitive indexes to stress by toxicants such as copper. The present findings suggest that simultaneous stress due to temperature change and copper exposure can accelerate changes in enzymes activities in the black rockfish. This provides another example of synergism between environmental temperature and pollutants, which may have important implications for the survival of fish in polluted environments during seasonal warming and/or global climate change.

수온 증가에 따른 말전복, Nordotis gigantea과 둥근전복, Haliotis discus discus (Reeve, 1846) hemolymph의 생리학적 변화

민은영,김신후,황인기,김경욱,박보미,이정식,강주찬 한국패류학회 2015 The Korean Journal of Malacology Vol.31 No.3

This study was conducted to examine the effects of alterations in water temperature (WT) on biochemical and immunological factors in the hemolymph of the abalones, Nordotis gigantea and Haliotis discus discus. The abalone were exposed to various WT; 18, 20, 22, 24, 26 and 28℃ for 96 hours. In biochemical factors, total-protein (TP), glucose, magnesium (Mg), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were not significant changes in hemolymph of N. gigantea and H. discus. But calcium was significantly increased by high WT (≥ 24℃). In immunological factor, The phenoloxidase (PO) activity was decreased in hemolymph of N. gigantea and H.D. hannai exposed to high temperature (≥ 22℃) compared to the control (P < 0.05). Whereas alkaline phosphatase (ALP) was not significantly changed. These results suggested that high temperature adversely affects the immunity of N. gigantea and H.D. hannai.

Changes in Antioxidant Enzyme Activities in the Gill And Digestive Glands of the Manila Clam Ruditapes philippinarum exposed to Cu

민은영,장석우,김성길,강주찬 한국수산과학회 2010 Fisheries and Aquatic Sciences Vol.13 No.1

We investigated the effect of Cu exposure on the activities of protective antioxidant enzymes in the gills and digestive glands of the manila clam Ruditapes phillippinarum exposed to sub-chronic concentrations (0, 20, 40, and 80 µg L-1) of waterborne Cu. No mortality occurred during the experimental period, and no significant condition index differences were observed in any exposure group compared with the control. No significant differences were observed in the digestive glands and gills of the clams observed during 15 days of exposure, but after 30 days, the SOD activity in the gill showed a significant difference between the 80 μg L-1 Cu-exposed group and the control. GPx activities in the digestive glands and gills were significantly lower after 30 days of Cu exposure. Gill GR activity in the high-exposure group (80 μg L-1) was significantly elevated compared with that in the control group. GST activities in the digestive glands of all groups did not change over 30 days. However, GST activity in the gill at 80 μg L-1 Cu was significantly higher after 15 and 30 days of exposure. GSH activities in the gill showed patterns similar to those of GST activities during exposure periods. In the digestive glands, GSH activity was higher only at 80 μg L-1 after 30 days exposure. In digestive glands and gills, the MDA levels of clams exposed to 80 μg L-1 Cu were significantly higher after 30 days of exposure.

Effects of Cadmium on Embryo Hatchability, Larval Development and Survival of the Olive flounder, Paralichthys olivaceus

민은영,강주찬 한국어병학회 2013 한국어병학회지 Vol.26 No.1

The cadmium (Cd) toxicological effects on the fertilized eggs, embryos and larvae were investigated in olive flounder, Paralichthys olivaceus water-borne exposed to Cd. The survival rate and hatching success of the embryos significantly diminished in treated groups in dependence of the Cd concentration. Significant differences were found at ≥ 30 µg L-1 compared to the control. A significant increase of malformation of the embryo was observed at ≥20 µg L-1 groups. They usually include such symptoms as clouded yolk-sac abnormality, fin erosion and spinal curvature. A significant reduction in the survival rate of the larvae was observed in ≥20 µg L-1 groups with accompanied by the disorder. Notably, in larvae, a concentration as low as 10 µg L-1 caused significant elevated abnormalities that is incidences of spinal cord deformation, abnormal eyes, deformation of the head region and severe developmental delay.

넙치, Paralichthys olivaceus의 수정난과 부화자어에 미치는 저염분의 영향

민은영,이옥현,강주찬 한국어병학회 2007 한국어병학회지 Vol.20 No.3

The hypo-salinity effects on fertilized eggs, embryos and larvae were investigated in olive flounder (Paralichthys olivaceus) obtained from hatcheries in Cheju-Island, Yeosu and Chungnam. Those were treated to eight concentration; 0, 3.4, 6.7, 10.1, 13.4, 20.2, 27.4 and 33.6 ‰. It was not discrepancy in the survival rate and hatching success rate of fertilized embryos obtained from different regions. Also, in the larvae, the regional difference was not appeared. The survival rate and hatching ability of embryos significantly diminished in the lower groups than 13.4 ‰ compared to 33.6 ‰. After fertilization, namely embryos are tolerant of a wide range of salinity (13.4 - 33.6 ‰). Reduced salinity induced an increase of the malformed embryo and larvae including various deformities; irregular embryos membrane (or yolk sac depression), fin erosion and swim bladder inflation in the flounder embryo. The hatching success of embryos was significantly reduced in lower salinity than 13.4 ‰. Notably, the reduction of larval survival rate significantly was observed in ≤10.1 ‰ treated groups with the same manner of survival rates of the embryos. Additionally, olive flounder was found to be adequate model for measuring external impulses because there are no the regional differences.

Toxicological Efects of Aroclor 1254 on the Embryonic Development of the Olive Flounder Paralichthys olivaceus

민은영,강주찬 한국수산과학회 2013 Fisheries and Aquatic Sciences Vol.16 No.4

We investigated the toxicological effects of Aroclor 1254 on the fertilized eggs, embryos and larvae of the olive flounder Paralichthys olivaceus. The survival rate and hatching success of the embryos decreased significantly in treated groups in an Aroclor1254-dose-dependent manner. Significant differences were found at ≥5 μg/L Aroclor 1254 compared to the control group. Hatching success occurred at ≤10 μg/L Aroclor 1254, which was not significantly different to the control. Embryo malformationincreased significantly at ≥1 μg/L, and included yolk-sac and tail-flexure abnormalities. There was a significant decrease in the survival rate of the larvae at ≥5 μg/L, which was accompanied by the malformations described above. Notably, concentrations as low as 1 μg/L caused a significant increase in abnormalities in the larvae, including incidences of multi-focal hemorrhages, pericardial and yolk-sac edema, inhibition of swim bladder inflation and severe developmental delay. The responses to Aroclor 1254-induced toxicity were generally similar among fertilized eggs, embryos and larvae from three separate flounder hatcheries: Cheju Island, Yeosu and Chungnam, South Korea. These results indicate the high acute toxicity of Arolcor 1254 concentrations of which as low as 1 μg/L in olive flounder larvae can affect unhatched embryos. To conclude, the average LC50 values for Aroclor 1254 in the embryos and larvae were 50.92 and 3.08 μg/L, respectively. Additionally, the average EC50 values, based on the rate of damage were 14.72 and 5.61 μg/L, respectively.

Fluctuation of Temperature Induces Pathogenicity of Streptococcus iniae and Changes of Immunology Related Genes of Korean Rockfish, Sebastes schlegeli

민은영,정선명,Hyun-Ja Han,조미영 한국하천호수학회 2023 생태와 환경 Vol.56 No.4

This study was designed to examine the immune response in Korean rockfish during water temperature fluctuation and to elucidate the factors contributing to streptococcal pathogenesis in cultured Korean rockfish, S. schlegeli. We investigated cumulative mortality against Streptococcus iniae (FP5228 strain) infection in the exposed Korean rockfish (39.7±5.8 g) to environmentally relevant temperature (Control, 23℃; High temperature, 28℃ and 23℃ and 28℃ with 12 hours interval exchange, 23↔28℃) for 48 hours. Also, the expression of the mRNA related to the immune response genes (heat shock protein 70, interleukin- 1β, lysozyme g-type and thioredoxin-like 1) were measured in spleen and head kidney by real-time PCR analysis in the exposed fish to thermal stress. In this study, the combined stress with bacterial challenge in fishes exposed to thermal stress lowered the survival rate than that of control (23℃). The cumulative mortality in the group of control, 28℃ and 23↔28℃ was 24%, 24% and 40% (P<0.05), respectively. Also, thermal stress modulated the mRNA level of immune related genes; heat shock protein 70, interleukin-1β, lysozyme g-type and thioredoxin-like 1 in Korean rockfish. The present study indicates that a high and sudden water temperature change affect immune responses and reduce the disease resistance in Korean rockfish.