옥수수 대로부터 생리활성물질 생산 증대를 위한 마이크로파 추출 공정 최적화

민보라,한여정,이도경,조재민,정현진,김진우,Min, Bora,Han, Yeojung,Lee, Dokyeoung,Jo, Jaemin,Jung, Hyunjin,Kim, Jin-Woo 한국화학공학회 2018 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.56 No.1

옥수수 대는 셀룰로오스와 헤미셀룰로오스 이외에 높은 함량의 리그닌을 포함하고 있어 리그닌 분해를 통해 폴리페놀 생산이 가능하여 천연 항산화물 생산이 가능한 후보이다. 옥수수 대로부터 마이크로파 전처리를 이용해 폴리페놀과 플라보노이드 추출 증대를 위해 반응표면분석법(RSM)을 이용하여 공정조건을 최적화하였다. 폴리페놀과 플라보노이드의 함량은 마이크로파 출력과 추출 시간 증가에 따라 유의하게 증가하는 경향을 보였다(p<0.05). 조건 최적화에 있어 698.6 W, 240 sec, 0 mol 조건에서 최대 TPC 82.4 mg GAE/g DM과 플라보노이드 18.1 mg QE/g DM이 예측되었다. 기존 추출법인 속실렛과 마이크로파 추출을 비교하였을 때, 마이크로파 추출이 폴리페놀과 플라보노이드 생산에 있어 13.5와 8.0배가 각각 높고 짧은 추출 시간과 낮은 에너지 소비로 기존 추출 대비 효과적인 공정임을 확인하였다. 본 연구는 옥수수 대로부터 유용물질 생산 가능성과 마이크로파 추출법이 상업화 공정 적용이 가능한 효과적인 추출법임을 확인하고 옥수수 대를 이용한 폴리페놀과 플라보노이드 생산을 통해 바이오당 생산과 더불어 부산물 크레딧을 확보하여 바이오 에탄올 가격 경쟁력을 높일 수 있는 추출공정을 제안하였다는 데 의의가 있다고 하겠다. Corn stover is known as a good candidate for a functional food ingredient when the main lignocellulosic material, lignin, is used as bioactive materials as form of polyphenolic compounds. The purpose of this study was to determine the microwave extraction conditions under which total phenolic compounds (TPC) and flavonoid contents of corn stover were maximized. Microwave-assisted extracts using sulfuric acid ranging from 0 to 1.0 mol with extraction time between 40 and 240 sec were conducted by using response surface methodology (RSM). Microwave power showed significant effects (p<0.05) and the concentrations of TPC and flavonoids increased with increased level of microwave power and extraction time. The optimum conditions for corn stover extraction were determined as 698.6 W, 240 sec, and 0 mol sulfuric acid, and the predicted value of TPC and flavonoid is 82.4 mg GAE/g DM and 18.1 mg/g DM, respectively. Microwave extraction was evaluated as an economic process with low energy consumption, short extraction and high extraction yield of bioactive including TPC and flavonoids compared to conventional extractions.

보문 : 조선시대 펠트(felt)의 사용범위와 특성

민보라 ( Bo Ra Min ),홍나영 ( Na Young Hong ) 韓國衣類學會 2008 한국의류학회지 Vol.32 No.10

보문 : 조선후기 감로탱화를 통해서 본 무녀복식에 관한 연구 (제2보)

민보라 ( Bo Ra Min ),홍나영 ( Na Young Hong ) 韓國衣類學會 2007 한국의류학회지 Vol.31 No.8

조선후기 감로탱화 하단화를 통해 본 예인복식 연구(제1보) - 사당패 남자복식을 중심으로 -

홍나영,민보라,Hong, Na-Young,Min, Bo-Ra 한국의류학회 2006 한국의류학회지 Vol.30 No.1

This study is focused on the man's costumes of Sadangpae(the troupe of performers) shown in Gamrotaenghwa (감로탱화) during the late Chosun dynasty. Originally Gamrotaenghwa was used as a painting for a Buddhist praying ceremony, 또Young-ga-cheon-do-je(영가천도제). It shows us the lives of the commoners in those days. Gamrotaenghwa reflects the changes of costume style in the late Chosun period. The early styles of po(포, coat) for male performers in the 17th century were changeui(창의) and dopo(도포), which had the front panels(seop) overlapped deeply and sleeves that got wider as time passed. After the 18th century, Male performers wore a simpler coat such as sochangeui(소창의), which had narrow sleeves and long slits on the both sides. Especially the coats of acrobats were fastened on the center front with buttons. Heuklip(흑립: black hat), somoja(소모자: small cap) and jeonlip(전립: wool hat) were used as hats for the male performers. Originally, heuklip was the hat that represented the noble status: yangban(양반). However, it was popularized among the commoners in the late Chosun period. As time passed on, the heuklip became more popular and its shape also changed. Somoja and jeonlip were shown throughout the entire Gamrotaenghwa. Unlike heuklip, those were common hats for Sadangpae. The costumes of entertainers shown in Gamrotaenghwa were very similar to those of the commoners. However, it seems that there were some differences of the costumes depending on the roles they performed.

조선후기 甘露幀畵 下段畵를 통해서 본 藝人복식 연구 (제1보)-사당패 남자복식을 중심으로-

홍나영,민보라 한국의류학회 2006 한국의류학회지 Vol.30 No.1

This study is focused on the man's costumes of Sadangpae(the troupe of performers) shown in Gamrotaenghwa (甘露幀畵) during the late Chosun dynasty. Originally Gamrotaenghwa was used as a painting for a Buddhist praying ceremony, Young-ga-cheon-do-je(靈歌天度齎). It shows us the lives of the commoners in those days. Gamrotaenghwa reflects the changes of costume style in the late Chosun period. The early styles of po(袍, coat) for male performers in the 17th century were changeui( 衣) and dopo(道袍), which had the front panels(seop) overlapped deeply and sleeves that got wider as time passed. After the 18th century, Male performers wore a simpler coat such as sochangeui(小 衣), which had narrow sleeves and long slits on the both sides. Especially the coats of acrobats were fastened on the center front with buttons. Heuklip(黑笠: black hat), somoja(小帽子: small cap) and jeonlip(戰笠: wool hat) were used as hats for the male performers. Originally, heuklip was the hat that represented the noble status: yangban(兩班). However, it was popularized among the commoners in the late Chosun period. As time passed on, the heuklip became more popular and its shape also changed. Somoja and jeonlip were shown throughout the entire Gamrotaenghwa. Unlike heuklip, those were common hats for Sadangpae. The costumes of entertainers shown in Gamrotaenghwa were very similar to those of the commoners. However, it seems that there were some differences of the costumes depending onthe roles they performed.

건설폐기물(建設廢棄物) 재활용(再活用) 과정에 대한 경제성(經濟性) 평가(評價) 연구(硏究)

박원우,이상덕,민보라,박이란,김의경,백미화,김동수,Park, Won-Woo,Lee, Sang-Duck,Min, Bo-Ra,Park, Lee-Ran,Gim, Ui-Gyeong,Baek, Mi-Hwa,Kim, Dong-Su 한국자원리싸이클링학회 2008 資源 리싸이클링 Vol.17 No.2

산업화 과정에서는 항상 폐기물이 발생하며 그 양은 점차 증가하고 있다. 2003년 생활 및 사업장 폐기물은 전년대비 9.5%의 증가추이를 보인 반면, 건설 폐기물은 전년대비 21.0%의 높은 증가 추이를 보였다. 현재 전체폐기물 중 건설 폐기물은 50%에 육박하며, 그 중에서도 폐콘크리트와 폐아스콘이 73%를 차지하고 있다. 이에 더하여 지금까지 풍부한 천연자원으로만 여겨졌던 골재의 양이 점차 줄어들고 있어, 건설 폐기물 재활용의 필요성은 더욱 강조되고 있다. 재생골재의 활용은 막대한 건설폐기물의 처리를 용이하게 함과 동시에 골재채취로 인한 환경파괴는 물론 부족한 신규골재를 대체할 수 있는 효과를 가진다. 본 연구는 건설 폐기물중 배출량이 상대적으로 많은 폐아스콘을 중심으로 재활용 경제성 분석을 위해 비용/편익 모형을 사용하였으며, 신규골재와 재생골재간의 비용 비교만을 수행하였다. 이 식을 이용하여 2003년과 2004년을 기준으로 한, 수도권 지역 내 아스콘에 대한 재활용 경제성을 평가해 본 결과, 2003년의 0.0694보다 2004년이 0.0805로 더 높게 나타났다. 두 자료만으로 결론을 내릴 수 없으나, 이는 건설부문에서 아스콘의 재활용 가치가 높아짐을 반증하고 있다고 사료된다. Amount of waste is always generated in industrialization process and it is gradually increasing. Domestic and industrial waste in 2003 increased by 9.5 percent than that of the last year(2002), whereas the amount of construction waste increased largely by 21 percent. Recently construction waste of total waste accounts for nearly 50 percent, waste concrete and Ascon from the construction waste takes up to 73 percent. Furthermore, amount of natural materials are gradually decreasing, that is, they are not sufficient any more. Owing to these reasons, the importance of recycling construction waste has been emphasized. The use of recycling aggregate makes the disposal of construction waste easier as well as protects environment from gathering raw aggregate. Also, it has the alternative effect economizing the insufficient new natural aggregate. This study employs the cost-benefit model to analyze the economic effect of construction waste recycling of Ascon which takes relatively high part of the total waste. The cost-comparison between raw aggregate and recycling aggregate were analyzed. With the model, the economic effect of Ascon recycling in 2003 and 2004 in capital area of Korea were analysed. Cost comparison between raw aggregate and recycling aggregate were also carried out. The result showed that the economic effect of Ascon recycling increased to 0.0808 for 2004 as compared 0.0694 for 2003. We could not conclude using above data, but this result shows that the economic benefit of Ascon recycling of construction waste has increased.

마이크로파 추출공정에 의한 옥수수속대 생리활성물질 추출조건 최적화

정현진(Hyunjin Jung),민보라(Bora Min),김승기(Seungki Kim),조재민,서주현(Joohyun Seo),김진우(Jin Woo Kim) 한국생물공학회 2018 KSBB Journal Vol.33 No.1

This study investigated the optimization of extraction of bioactive compounds from corncob using various microwave extraction conditions. The central composite design (CCD) was applied to optimize microwave power, extraction time, and sulfuric acid concentration. Among tested variables, microwave power and extraction time showed significant effects on total polyphenol compounds (TPC) as well as flavonoid contents. However, the change in concentration of sulfuric acid only had a significant effect on extraction of flavonoid. The optimum conditions of microwave-assisted extraction (MAE) were 644 w, 66.3 sec, and 0.03 mol of sulfuric acid, and in this condition, maximum concentrations of 7.91 mg GAE/g DM of TPC and 0.79 mg QE/g DM of flavonoid were extracted. Antimicrobial activity of extracts from MAE, hotwater extraction (HWE), and ultrasound-assisted extraction (UAE) were measured by disk diffusion assay. HWE and UAE had no antimicrobial effects, whereas the antimicrobial effect of MAE has been identified. In conclusion, MAE was found to be effective in extracting bioactive compounds, including TPC and flavonoid from corncob despite short extract time and low extraction under 84 sec and 120℃, respectevely.

탈지미세조류의 무효소 당화를 위한 마이크로파 전처리 조건 최적화

정현진 ( Hyun Jin Jung ),민보라 ( Bora Min ),김승기 ( Seung Ki Kim ),조재민 ( Jae Min Jo ),김진우 ( Jin Woo Kim ) 한국화학공학회 2018 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.56 No.2

본 연구의 목적은 탈지미세조류(LEA) 세포벽 분해를 통한 바이오당 생산에 있어 당화효소 사용없이 마이크로파 전처리만을 이용하여 글루코오스와 자일로오스를 생산하는 것이다. LEA의 주성분인 셀룰로오스와 헤미셀룰로오스의 무효소 당화를 위해 산 가수분해 기반의 마이크로파 전처리 조건을 반응표면분석법을 이용하여 최적화하였다. 마이크로파를 이용한 무효소 당화 공정의 주요 변수는 마이크로파 출력(198~702 W), 전처리 시간(39~241 sec)와 황산 농도(0~0.1 mol)로 최적 조건 예측을 위해 중심합성계획법을 이용하여 2차 회귀함수를 도출하였다. 마이크로파 출력과 전처리 시간이 LEA로부터 육탄당(C6)과 오탄당(C5) 생산에 유의한 영향을 주는 변수이며 증가에 따라 육탄당과 오탄당 당화율이 증가하는 경향을 확인하였다. 육탄당과 오탄당 당화율 최대화를 위한 산 가수분해를 적용한 마이크로파 전처리 최적 조건은 마이크로파 출력 700W, 전처리 시간 185.7 sec와 황산 0.48 mol으로 육탄당 당화율 92.7%와 오탄당 당화율 74.5%가 예측되었으며 확인 실험을 통해 육탄당 당화율 94.2%와 오탄당 당화율 70.8%가 확인되어 예측의 유효성을 확인할 수 있었다. 이는 LEA의 셀룰로오스와 헤미셀룰로오스 당화를 위해 산 가수분해 적용 마이크로파 전처리만을 이용한 무효소 당화 공정 적용과 100 °C 이하의 낮은 온도와 짧은 전처리 시간 적용을 가능하여 기존 전처리 대비 효과적인 공정 임을 입증했다. The purpose of this study was to effectively produce the biosugar from cell wall of lipid extracted microalgae (LEA) by using microwave-assisted pretreatment without enzymatic hydrolysis process. Response surface methodology (RSM) was applied to optimization of microwave-assisted pretreatment conditions for the production of biosugar based on enzyme-free process from LEA. Microwave power (198~702 W), extraction time (39~241 sec), and sulfuric acid (0~1.0 mol) were used as independent variables for central composite design (CCD) in order to predict optimum pretreatment conditions. It was noted that the pretreatment variables that affect the production of glucose (C6) and xylose (C5) significantly have been identified as the microwave power and extraction time. Additionally, the increase in microwave power and time had led to an increase in biosugar production. The superimposed contour plot for maximizing dependent variables showed the maximum C6 (hexose) and C5 (pentose) yields of 92.7 and 74.5% were estimated by the predicted model under pretreatment condition of 700 w, 185.7 sec, and 0.48 mol, and the yields of C6 and C5 were confirmed as 94.2 and 71.8% by experimental validation, respectively. This study showed that microwave-assisted pretreatment under low temperature below 100 °C with short pretreatment time was verified to be an effective enzyme free pretreatment process for the production of biosugar from LEA compared to conventional pretreatment methods.

커피박으로부터 생리활성물질 생산 증대를 위한 열수추출 공정 개발

조재민 ( Jaemin Jo ),김승기 ( Seungki Kim ),민보라 ( Bora Min ),정현진 ( Hyunjin Jung ),한여정 ( Yeojung Han ),김진우 ( Jinwoo Kim ) 한국화학공학회 2017 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.55 No.3

커피침출 공정의 부산물인 커피박을 이용한 고부가가치 식품 및 화장품 소재 생산을 위해 열수추출 공정의 폴리페놀 추출인자(용매, 온도, 시간, 농도) 최적화를 수행하였다. 실험에 적용 된 모든 추출인자가 폴리페놀 생산에 유의한 효과가 있음을 확인할 수 있었고 NaOH 농도가 폴리페놀 생산에 미치는 효과가 가장 큰 것으로 평가되었다. 특히, 열수추출 보다는 산 또는 염기를 이용한 열수추출이 폴리페놀 추출 효과가 높았으며 NaOH 0.1 mol 첨가 시 증류수를 이용한 열수추출에 비해 1.5배 추출 효과가 증가하여 열수를 이용한 추출에 비해 염기-열수 추출이 보다 효과적임을 알 수 있었다. 폴리페놀 추출의 최적 조건인 100 ℃, 2 mol NaOH와 30 min 추출 조건에서 36.5 mg GAE/g DM를 얻을 수 있었다. 이는 최적화 이전의 열수추출에서 얻은 12.5 mg GAE/g DM 대비 2.9배 증가된 결과로 커피박은 항산화활성 등 기능성 강화 소재로 식품 및 화장품 산업에 활용이 가능할 것으로 판단된다. In this study, the optimization of extraction parameters (solvent, temperature, time, solvent concentration) for the maximization of polyphenol extraction was performed to produce value-added food and cosmetic additives using a byproduct of coffee extraction process (coffee residue). All of the extraction parameters evaluated in this experiment had significant effects on polyphenol extraction and the results showed the effect of NaOH concentration on the polyphenol production was most significant among tested parameters. Especially, hot water extraction using acid or base was effective rather than hot-water extraction and the addition of 0.1 mol of NaOH increased 1.5 times extraction concentration compared with hot-water extraction using distilled water. It was found that hot-water extraction with NaOH was more effective than hot-water extraction, and 36.5 mg GAE/g DM was obtained under optimum condition of 100 ºC, 2 mol of NaOH and 30 min. This result was 2.9 times higher than that of 12.5 mg GAE/g DM obtained from the hot-water extraction before optimization. Thus, coffee residue could be used for food and cosmetic industry as a high-value additive such as antioxidant.

탈지미세조류로부터 초음파추출을 이용한 항산화 물질 생산 공정 최적화

조재민 ( Jaemin Jo ),신슬기 ( Suelgl Shin ),정현진 ( Hyunjin Jung ),민보라 ( Bora Min ),김승기 ( Seungki Kim ),김진우 ( Jinwoo Kim ) 한국화학공학회 2017 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.55 No.4

초음파 추출법(Ultrasonic-assisted extraction, UAE)은 기존의 추출법 대비 높은 추출 효율과 짧은 추출시간으로 식물 세포벽으로부터 생리활성물질 추출 또는 분리에 효과적인 방법으로 인식되어 관심이 증대되고 있다. 본 연구에서는 탈지미세조류(Tetraselmis KCTC 12236BP)에서 폴리페놀 추출을 위해 UAE를 적용하여 총 폴리페놀(TPC) 생산에 미치는 주요 추출변수의 영향을 평가하였다. 추출변수의 최적화를 위해 입자크기, 고액비(L/S ratio), 에탄올 농도, 추출 온도 및 추출 시간을 요인으로 하여 순차적인 최적화를 진행하였다. 실험에 적용 된 모든 변수는 TPC 추출에 유의한 효과를 보였으며 추출 온도가 TPC생산에 가장 큰 영향을 미침을 확인 할 수 있었다. 최적 추출조건은 혼합 입자 사용 시, 10%고액비, 60% 에탄올, 추출온도 100℃와 추출시간 30 분을 적용한 추출에서 8.7 mg GAE/g DW를 얻을 수 있었다. 동일한 추출조건에서 열수추출과 UAE를 비교하였을 때 UAE에서 TPC 추출이 1.8배 증가함을 확인하였다. 본 연구를 통해 저온 및 짧은 추출시간을 적용한UAE가 기존의 열수추출 공정에 비해 LEA를 이용한 생리활성물질 생산에 보다 효과적임을 확인하였다. Ultrasound-assisted extraction (UAE) has attracted growing interest, as it is an effective method for the rapid extraction of bioactive compounds from plants with a high extraction efficiency comparable to the conventional extraction. In this study, UAE was used for the extraction of polyphenols from lipid extracted microalgae (Tetraselmis KCTC 12236BP) and the effects of five extraction variables on the total phenolic compounds (TPC) were studied. For the optimization of extraction parameters, particle size, solid-to-liquid (L/S) ratio, ethanol concentration, extraction temperature and extraction time have been examined as independent variables. All variables exhibited the significant effects on the extraction of TPC and extraction temperature showed the most significant effect among five variables. The optimal extraction conditions were the extraction using mixed particle, S/L ratio of 10%, ethanol concentration of 60%, extraction temperature of 100℃ and extraction time of 30 min, which gave the 8.7 mg GAE/g DW for TPC. Compared with conventional hot-water extraction, TPC extraction under UAE was increased by up to 1.8 fold with same extraction condition. This study showed that UAE under low temperature and short extraction time was proven to be an effective extraction process for TPC production from LEA compared to conventional hot-water extraction process.