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박송용,황규계,최문기,유연우,백승복,김경호 대한바이러스학회 1991 Journal of Bacteriology and Virology Vol.21 No.1
A human embryonic lung fibroblast cell line has been established to isolate and propagate varicella-zoster virus (VZV) from Korean patients. The cells were confirmed to be free from extraneous agents as well as mycoplasma. Chromosome analysis of the established cell line revealed that it is less variable than the control cell lines such as WI-38 and MRC-5 obstained from ATCC. From every vesicle fluids of seven varicella patients who were 1 to 4 years old, viruses could be isolated. After the propagation of the viruses, they were proved to be VZV by CPE, IFA, western blot analysis, and electron microscopic photographs. In addition, culture conditions for the multiplication of VZV isolated from Korean patients were established.
박송용 한국미생물학회 1992 微生物과 産業 Vol.18 No.2
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박송용,조도현,김경호 대한바이러스학회 1986 Journal of Bacteriology and Virology Vol.16 No.2
The influenza virus type, A/Kumamoto/37/79 (H1N1), A/Ishikawa/102/81 (H3N2), and B/Singapore/ 222/79, were inoculated into egg allantoic cavity and the egg allantoic fluids was harvested after 2days incubation at 34C. The influenza virus was purified with BaSO4, and futher purified by sucrose gradient ultracentrifugation. The purified virus was treated with ether in order to remove lipid-rich part from viruses. The following results were obtained. 1. The profiles of the sucrose gradient ultracentrifugation(SGUC) showed that the fractions corresponding to the 39%-45% of sucroseare highest in the HA titer. 2. Electron photographs indicated that the ether treated virus took a completely deranged form, compared with the regular granular form of the untreated virus. 3. SDS-PAGE along the purification revealed that after SGUC, the egg proteins were almost completely removed from the virus. 4. After the final vaccine were produced by the addition of formalin and other stabilizer, the HAI and potency test were carried out. The HAI titer of the final vaccine was almost simliar to the reference vaccine. When the potency test was carried out by the method of neutralizing antibody, the Edvalue of the final test vaccine was 5' , average value of three virus strains, whereas the ED value of the reference vaccine was 5' , average value of three virus strains. Based on data, therefore, the final test vaccine almost equivalent to the reference vaccine obtained from the Kitasato Institute, Japan.
김민재,박송용,김혜숙,이미현,신광순,김경호 대한바이러스학회 1987 Journal of Bacteriology and Virology Vol.17 No.2
Influenza virus strains recommended by WHO for influenza virus vaccine in 1986-1987 season [A/ Bangkok/10/83(.H,N,), A/Yamagata/120/86(H,N,), A/Fukuoka/c29/85(H,N,) and B/Ibaragi/2/85] were cultured and concentrated with BaSO chicken-RBC, PEG-6000 or ultrafiltration. And the further purification was performed employing controlled pore glass gel permeation chromatography(CPG-PC) or sucrose gradient ultracentrifugation(SGUC). The purity of virus preparations achieved by CPG-PC was comparable to that by SGUC. The methods of SUGC or CPG-PC can be suitable to mass-scale purification of influenza virus.
국내에서 분리한 수두바이러스의 Restriction Fragment Length Polymorphism분석
황규계,박송용,김성진,유연우,김경호 대한바이러스학회 1991 Journal of Bacteriology and Virology Vol.21 No.2
Varicella-zoster viruses(VZV) were isolated from vesicle fluid of seven korean patients who were 1 to 4 years old and propagated in human embryonic lung fibroblast. We classify the seven VZV isolates in 3 groups by restriction fragment length polyrnorphism(RFLP) analysis using restriction endonucleases-Bam HI, Bgl I, Sma I and Pst I . These 3 types of VZV isolates were different from Ellen strain and Webster strain obtained from ATCC. Compared with Oka strain, two types of them showed different RFLP pattern but the other type showed the same RFLP pattern with above four restriction endonucleases.
Hantaan Virus 중화항체가 측정을 위한 플라크 감소 중화시험법과 형광 항체 중화시험법의 비교
김혜숙,박송용,안창남,백승복,김경호 대한바이러스학회 1990 Journal of Bacteriology and Virology Vol.20 No.2
The production of neutralizing antibody in mouse serum immunized with Hantaan virus was investigated. Mouse sera were prepared at one week interval after 2-3 times immunization with Hantaan virus. The sera were titrated neutralizing antibody against Hantaan virus using plaque reduction neutralization and immunofluorescence neutralizing method in Vero E6 cells. In mice immunized with Hantaan virus, the titer of plaque reduction neutralization on Hantaan virus was 30-50. The same results was obtained with immunofluorescence neutralizing technique. In this study, it was observed that plaque reduction neutralizing technique shows almost same results as immunofluorescence neutralizing technique. But the immunofluorescence neutralizing technique was easier than the plaque reduction neutralizing technique for titration of neutralizing antibodies against Hantaan virus.