http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Streptomyces peucetius subsp . caesius 돌연변이주에 의한 doxorubicin 생산의 최적배양조건
김승욱,송수문,문순옥 ( Seung Wook Kim,Soo Moon Song,Soon Ok Moon ) 한국공업화학회 1997 공업화학 Vol.8 No.4
본 연구에서는 Streptomyces peucetius subsp. caesius 돌연변이주에 의한 doxorubicin의 생산에 있어서 배양조건 및 배지의 성분을 확립하여 doxorubicin의 생산성을 높이는데 목적이 있다. Doxorubicin 생산을 위한 최적 배지조성은 4% maltose, 0.5% HEPES, 0.02% K₂HPO₄, 0.01% MgSO₄로 나타났고, 가장 적합한 종균 접종량과 시기는 10%(v/v), 72시간이었다. Doxorubicin생산에 적합한 소포제를 찾기위해 여러 종류의 소포제를 배지에 첨가한 결과 가장 적합한 소포제는 KG(10% K+10% G)이었으며 최적농도는 0.01%이었다. 교반식반응기에서 배양할 경우 적합한 통기량은 1.5v/v min으로 최대 29mg/l의 doxorubicin을 생산하였고, 1.0v/v min의 경우에도 플라스크 배양보다 15% 증가된 23mg/l의 doxorubicin을 생산하였다. The production of doxorubicin by a mutant of Streptomyces peucetius subsp. cazesius was studied. The optimal culture conditions, such as inoculum size and medium composition were established to improve the productivity of doxorubicin. The optimal medium composition was found to be 4% maltose, 0.5% HEPES, 0.02% K₂HPO₄, 0.01% MgSO₄. As an antiform agent, 0.01% KG(10% Adekanol+10% Silicone) was suitable one among various agents. Culture was carried out in 2.5 L jar-fermenter with different aeration rates of 1.5, 1.0, and 1.5 v/v min. The maximum production of doxorubicin(29 mg/l) was obtained at 1.5 v/v min of aeration rate, and even at 1.0 v/v min, the production of doxorubicin was increased up to 15% compared with that of shake-flask culture.
가용성 난각막이 Fibroblast 세포의 증식 및 Type Ⅲ Collagen 생성에 미치는 영향
문순옥 수원대학교 기초과학연구소 1996 基礎科學論文集 Vol.5 No.-
In this study it has been demonstrated that egg membrane hydrolysate(EMH) is able to stimulate the proliferation of NIH 3T3 cells and increase the production of type Ⅲ collagen in NIH 3T3 and Malme-3 (human skin fibroblast) cells. The addition of EMH to quiescent NIH 3T3 cells resulted in an increase of proliferation which was assayed by MTT method. The maximum effect of EMH was detected in 0.4% EMH treated cells which was comparable to that of 5% serum (96% of 5% serum effect). The addition of EMH to NIH 3T3 and Malme-3 cells also increased the production of type Ⅲ collagen in both cells. These results indicate that EMH may enhance the repair process after injury and prevent aging processes in connective tissues.
펩타이드 성장인자의 second Messenger가 NIH 3T3 세포증식에 미치는 영향
文順玉 水原大學校 1992 論文集 Vol.10 No.-
Various peptide growth factors bind to their specific cell surface receptors and produce second messengers which transduce the growth signal into the cytoplasm and nucleus. To study the effects of these second messengers on the cell proliferation, tumor promotor TPA, calcium ionophore A23187 and dibutyl cAMP were used. Among These agents TPA showed strongest stimulation on the proliferation of NIH 3T3 cells and also synergistic effect with dibutyl cAMP. But these effects were eliminated by amilolide, ?? antiporter inhibitor which implied that TPA and dibutyl cAMP transfer the signal to the ?? antiporter. And this signal is important for the cell proliferation.
Colo 320 세포에서 Streptomyces sp. No.2303 배양추출액의 항암활성 작용기작의 규명
문순옥 수원대학교 자연과학연구소 1998 자연과학논문집 Vol.1 No.-
Streptomyces sp. No. 2303 was selected previously by its cytotoxic effect against human lung carcinoma A549 cells and its inhibitory activity to expression of oncogene, c-myc. In this study, the cytotoxic effect of N0.2303 on c-myc amplified human cancer cell, Colo320 DM and HSR was performed by using 3-(4,5-dimethyltiazol-2-yl)2, 5-diphenyltetrazolium bromide(MTT) assay. Culture extract of No.2303 showed cytotoxic effect and induced DNA fragmentation which is hallmark of apoptosis in Colo320 DM and HSR cells. To elucidate the action mechanism of culture extract of No.2303, the effect of messengers of polypeptide growth factors on the anticaner activity of No.2303 was investigated. 12-0-tetradecanoyl phorbol-13-acetate(TPA) and dibutyryl cAMP(db cAMP) plus 3-isobutyl-1-methylxanthine(IBMX) were used as second messengers of growth factor because they act like diaclyglycerol(DAG) and cAMP respectivly inside of the cells. Anticancer activity and apoptosis induced by No.2303 were inhibited by treatment with TPA and db cAMP plus IBMX. These results imply that No.2303 induce anticancer activity by inhibiting c-myc expression and inducing apoptosis through pathway which involves the activation of protein kinase C(PKC) and protein kinase A.
In Vitro에서의 항암물질 Screening 방법에 대한 연구
文順玉 수원대학교 산업기술연구소 1991 산업기술연구소논문집 Vol.6 No.-
Screening method for the isolation of anticancer molecule from microbial extract was entablished by using sulforhodamine B(SRB) assay, Hep-2 and PC14 cell lines which were derived from human epidermoid carcinoma and prostate adenocarcinoma respectively. The effect of cell number on SRB assay showed the absorbance is proportional to cell number. The growth rates of cell lines were determined using the SRB assay. These results can be used for the optimization of screening test. Primary screening test showed that extracts of Actinomycetes isolated from soil have cytotoxic activity to PC14 and Hep-2 cell lines.
세포질의 알카리화가 Fibroblast Monolayer에 미치는 Mitogenic Effect
文順玉 水原大學校 1989 論文集 Vol.7 No.-
Stimulation of ?? exchange by growth factors has been implicated as an event required for progression of cells from quiescence to a state competent for proliferation. To test this hypothesis, confluent monolayers of mouse and human fibroblasts, were treated with the carboxylic ionophore monensin which catalyzes ?? exchange at the plasma membrane. When ?? monensin is incubated with cells DNA synthesis is stimulated. Growth factors that stimulate ?? exchange also induce expression of two cellular proto-oncogenes, c-fos and c-myc. These gene products have been implicated as regulators of proliferation of mammalian cells. Transient exposure of quiescent Balb/c-3T3 cells to monensin stimulates expression of both c-fos and c-myc proto-oncogene. These data suggest that stimulation of ?? exchange regulates the level of mRNA decoding for c-fos and c-myc and provide a key stimulus for cell proliferation.