Bacillus sp. Ye-1213이 생산하는 chitinase의 동정, 분리정제 및 특성

오두환,허남윤,백무열 연세대학교 산업기술연구소 1990 논문집 Vol.22 No.1

국내 해변 연안의 해변의 토양으로부터 chitinase 활성이 우수한 균주를 분리하고 동정하여 Bacillus sp. YE-1213으로 명명하였다. Chitinase를 생산하기 위한 최적 배양조건은 1.0%(w/v, dry base) Colloidal chitin, 1.0%(w/v) Tryptone, 0.05%(w/v) MgSo₄·7H₂O, pH 8.0, 30℃, 배양시간 48시간이었다. 효소는 (NH₄)₂SO₄분별침전, affinity adsorption, Sephadex G-200 gel filtration의 과정을 거쳐 정제한 결과 비활성 81.32units/mg, 정제도 16.7배, 수율은 15.8%이었다. 정제된 효소는 최적 반응조건은 pH 6.0, 40℃ 이었으며, 온도에 대한 효소안전성은 40℃까지는 매우 안정하였고 pH에 대한 안정성은 pH 5.0-9.0에서 안정하였다. 정제된 효소의 Km 값은 1.74mg/ml이었고 PCMB, I₂, ?? ion, ?? ion 에 의해 저해되었으며 ?? ion, ?? ion 및 ?? ion 에 의해 1.21배, 1.12배, 1.18배의 효소활성이 증가하였다. A bacterium with the highest chitinase activity selected from the soil and identified as a Bacillus sp. YE-1213. The optimal condition for the production of chitinase was as follows; 1.0% (w/v, dry base) colloidal chitin, 1.0%(w/v) tryptone, 0.05%(w/v) MgSO₄·7H₂O, pH8.0, 30℃, 48 hrs. The chitinase was purified about 16.7 folds by ammonium sulfate fraction, affinity adsorption and Sephadex G-200 gel filtration. Optimal temperature and pH for the enzyme activity were 40℃ and pH 6.0. The enzyme was stable between pH 5.0-9.0 and up to 40℃. Its activity was activated by ??, ??, and ?? while inhibited by PCMB, I₂, ?? and ??.

Plasmid DNA pBR 322의 증폭과 Hydroxyapatite Chromatography에 의한 분리

오두환,유주현,신원철,정건섭,유승구 연세대학교 산업기술연구소 1984 논문집 Vol.16 No.1

The amplification and the isolation conditions of pBR322 plasmid DNA were investigated. For the amplification of pBR322 plasmid DNA, cells from logarithmic growth phase were most effective. The optimal conditions for the amplification of pBR322 plasmid DNA in Escherichia coli GM4 were obtained as follows; 50-150 ㎍/ml of chloramphenicol concentration, 6-8 hours of incubation time in the presence of chloramphenicol. Ampicillin and tetracycline had no effect on the amplification of pBR322 plasmid DNA. Approximately 1 mg of pBR322 plasmid DNA was purified from 3.5 ℓof Escherichia coli GM4 culture broth by hydroxyapatite chromatography. The purified pBR322 plasmid DNA was expressed in Escherichia coli C600.

변이주인 Trichoderma reesei YC-3156이 생산하는 Cellulase에 관한 연구

오두환,안순철,유주현,허남윤 연세대학교 산업기술연구소 1987 논문집 Vol.19 No.2

After the treatment 15 strains of Trichoderma sp. and 4 isolates with N-methyl-N´-nitro-nitrosoguanidine, T. reesei YC-3156 among the mutants showed the highest cellulase activity. The Avicelase of T. reesei YC-3156 was purified by 70% ammonium sulfate fractionation, dialysis, DEAE-Sepharose column chromatography and Sephadex G-100 Gel-filtration. With the result of the procedures, its purification degree was 13 folds, yield 9.3% and specific activity 0.9 unit/mg. The homogeneity and the molecular weights of the purified Avicelase were determined by SDS-polyacrylamide gel electrophoresis, it was electrophoretically homogeneous and showed the molecular weights of 46,000-48,000. The purified Avicelase was stable between pH 4.0 and pH 5.0 and it was inactivated rapidly above 60℃.

Streptobacillus sp.가 생산하는 pullulanase에 관한 연구

오두환,지희숙,최형택,신원철,유주현 연세대학교 산업기술연구소 1986 논문집 Vol.18 No.2

A bacterium with the highest pullulanase activity was isolated from the soil and identified as Streptobacillus sp. Y-716. The optimum culture condition of Streptobacillus sp. for the production of pullulanase was as follows; 1.2%(w/v)soluble starch, 0.7%(w/v) CH₃COONH₄, 0.05%(w/v) MgSO₄7 H₂O, 0.01%(w/v)FeSO₄7H₂O, at 30℃, for 48 hrs. The pullulanase from the culture broth of Streptobacillus sp. Y-716 was purified about 46 fold by ammonium sulfate precipitation, dialysis. DEAE-cellulose and Sephadex G-200 column chromatography. The purified enzyme was stable between pH 5.0 and pH 7.5. It was inactivated rapidly above 35℃. The Km value and Vmax of the purified enzyme were 0.0137mg/m/ and 0.666μm/min. on pullulan, respectively.

수출입 컨테이너 장치장 배정을 위한 소프트웨어의 개발

김갑환,김홍배,홍봉희,김기영,배종욱,최진오,김두열,이영기,박영만,박강태,손행대 한국경영과학회 1995 經營 科學 Vol.12 No.3

The Pusan Container Terminal faces a rapid increase in berthing time of container ships as well as in waiting time of external trucks, which is due to an absolute lack of yard space. This research is focused on the development of a decision support system for the planning of the container terminal yard assignment so that the yard space would be utilized most effectively. Efforts should be given to the reasonable assignment of the yard storage and the dynamic adaptation to the ever changing environment. The software introduced here is based o the know-how of the field exports and its framework takes the approach of the hierarchical decision making.

승객용 엘리베이터 와이어로프의 유효수명 및 교체시기 평가

손두익,박재석,오환섭 한국안전학회 2003 한국안전학회지 Vol.18 No.4

The wire rope, one of the component of the elevator, is extremely important for the safe operation of the elevator. That is why the wire rope is replaced at a specific interval regardless of the operating conditions or the degree of damage to prevent the breakage of the wire rope. This is the waste of resources, causing economic and environmental loss. This paper has surveyed and analyzed the status of replacing wire-rope by buildings that use elevators, in order tc compare the replacement cycles of the wire rope under each operating conditions.

태양추적장치의 상태공간 적용분석

안두수,유상진,오민환 成均館大學校 科學技術硏究所 1990 論文集 Vol.41 No.2

The purpose of a solar tracking system is to control the attitude of a space vehicle so that it will track the sun with high accuracy. In this paper, tracking of the sun in one plane is accomplished, an iterative procedure for control modellig of solar tracking system is presented by simultaneous differential equations and transfer functions. Also a program for obtaining a state-space representation is developed. A program for computation of state variables is based on the use of a 4th-order Runge-Kutta numerical integration algorithm included subroutines. It is applicable for the nonlinear time-variant case by appropriate modification of the subroutine. Computer simulation results are shown for the dynamic characteristics of vehicle system, armature -controlled DC motor and controller. Althogh certain interesting results are well established and documented, it is still a difficult task in the hardware realization.

改正信用狀統一規則의 運用上의 問題點에 관한 硏究 : 運送書類關聯規定을 中心으로

朴斗學,梁暎煥,吳元奭,金正秀 東亞大學校 大學院 1987 大學院論文集 Vol.12 No.-

The U.C.P(1983 Revison) set out guidelines relating to a transport document which would enable banks to accept such a document, or compel them to reject it, regardless of what the document appers to call itself, as follows: a) The case where the credit stipulates a marine bill of lading as the required transport document(Article 26) or, b) The case where the credit stipulates dispatch of goods by post(Article 30), of which case was excluded in this thesis or, c) All other cases where the credit calls for a transport document(Article 25). The definitions of the "shipment" are of "loading on board" or "dispatch" or "taking in charge", so Article 25 which made a clear distinction betwen a marine bill of lading and other transport document, especially combined transport document, referred mainly to the combined transport document as a popular transport document into four paragraphs: Paragraph(a): "mandatory general requirement" which the transport doucument must need for tis to be acceptable under the credit: Paragraph(b): "optional extras", i.e. additional elements in the transport document which will not affect its acceptability; Paragraph(c): elements in the transport document which make its rejection mandatory such as charter-party B/L, or the document issued by sailing vessel; Paragraph(d): elements in a transport document which may cause it to be rejected, such as freight forwarder's transport document. or which may permit its acceptance, such as FIATA FBL, including the freight forwarder's transport document acting as a carrier or agent of a named carrier. In making application of this paragraph(d) in practical side, what is mattered is that the U C P (1983 revision) did not provide the definition of a "carrier" which may be a norm in deciding of acceptability of the freight forwarder's transport document. The 1980 Revision of Incoterms, appreciating that one of the basic functions of a transport document is to evidence the contract of carriage, states that "carrier" means any person by whom or in whose name a contract of carriage by road, rail, sea or a combination of modes has been made, but this definition does not have a binding force and effect on the L/C tranaction because the L/C is a separate transaction because the L/C is a separate transaction from the sales contract. Article 26 applies only when the credit calling for a transport document stipulates as such document "a marine bill of lading", and it broadly follows the structure of Article 25. A marine bill of lading in the Article, unkess otherwise stupylated in the credit must be an "on board" form. As this Article only apply to " a marine bill of lading", the document which contains the word "intended" or "issued by freight forwarder", unless it indicates that the freight forwarder is acting as a carrier or its agent, will not be acceptable. But the Paragraph(b) in this Article stipulates that banks will not reject a document which bears a title such as "Combined transport billof Lading", Combined transport doucument", "Combined transport bill of lading or port-to-port bill of lading ......, so in cases of Combined transport documents which meet the requirements of Paragraph(a) of article 26, it is very confusing to find a appropriate Article in application. thus to avoid a confusion L/C applicant showed designate Article 26 in L/C if he wants it to be applied in Combined transport document. Article 27 made the change of UCp(1974 revision) that an "on board" document should be the norm; namely "taken in charge" or "received for shipment" now because an equivalent alternative for transport documents other than marine bill of lading.

간접적응 신경회로망을 이용한 안테나 축받이 시스템의 안정화

이오걸,김태우,박두환,이준탁,송효신 동의공업대학 1999 論文集 Vol.25 No.1

In this paper, the stabilization control problems of antenna pedestal system under the parametric variations and non-linearities such as gravity torque and under external disturbances were described. The proposed controller could stabilize the given antenna system by an indirect adaptive neural network controller. The proposed indirect adaptive neural network controller was consist of an indirect off-line model identifier by neural network and an adaptive on-line neural network controller, based on the reference model and the least square method. The designed controller was applied to an stabilization control of Inmarsat-M antenna pedestal system with the azimuth and the elevation axis. The simulation results were desirable and were superior to the conventional PID controller tuned by Ziegler-Nichols' method.

월쉬변환에 의한 시지연계의 간단화 모델링 및 최적제어에 관한 연구

안두수,오민환,채영무 成均館大學校 科學技術硏究所 1992 論文集 Vol.43 No.1

시지연계를 지연이 없는 선형계로 모델링 할 경우에는 고차 방정식으로 표현되기 때문에 실제로 제어하기는 어렵다. 본 연구에서는 시지연요소를 직접 처리하지 않고 지연이 없는 형식을 이용하여 모델을 간략화 시키므로서 시지연계의 최적제어를 간단히 할 수 있도록 하였다. It is difficult to analyze time delay system because the system is commonly expressed by high order equations. To avoid the difficulty, the undelayed linear formulations have been used. However, it is time consuming to directly analyze the undelayed linear formulations in case of complicated system. In this paper, to prevent disadvantage of time consuming, a proper solution technique having advantage of time saving is used to solve the system.