펩타이드 성장인자의 second Messenger가 NIH 3T3 세포증식에 미치는 영향

文順玉 水原大學校 1992 論文集 Vol.10 No.-

Various peptide growth factors bind to their specific cell surface receptors and produce second messengers which transduce the growth signal into the cytoplasm and nucleus. To study the effects of these second messengers on the cell proliferation, tumor promotor TPA, calcium ionophore A23187 and dibutyl cAMP were used. Among These agents TPA showed strongest stimulation on the proliferation of NIH 3T3 cells and also synergistic effect with dibutyl cAMP. But these effects were eliminated by amilolide, ?? antiporter inhibitor which implied that TPA and dibutyl cAMP transfer the signal to the ?? antiporter. And this signal is important for the cell proliferation.

In Vitro에서의 항암물질 Screening 방법에 대한 연구

文順玉 수원대학교 산업기술연구소 1991 산업기술연구소논문집 Vol.6 No.-

Screening method for the isolation of anticancer molecule from microbial extract was entablished by using sulforhodamine B(SRB) assay, Hep-2 and PC14 cell lines which were derived from human epidermoid carcinoma and prostate adenocarcinoma respectively. The effect of cell number on SRB assay showed the absorbance is proportional to cell number. The growth rates of cell lines were determined using the SRB assay. These results can be used for the optimization of screening test. Primary screening test showed that extracts of Actinomycetes isolated from soil have cytotoxic activity to PC14 and Hep-2 cell lines.

Colo 320 세포에서 Streptomyces sp. No.2303 배양추출액의 항암활성 작용기작의 규명

문순옥 수원대학교 자연과학연구소 1998 자연과학논문집 Vol.1 No.-

Streptomyces sp. No. 2303 was selected previously by its cytotoxic effect against human lung carcinoma A549 cells and its inhibitory activity to expression of oncogene, c-myc. In this study, the cytotoxic effect of N0.2303 on c-myc amplified human cancer cell, Colo320 DM and HSR was performed by using 3-(4,5-dimethyltiazol-2-yl)2, 5-diphenyltetrazolium bromide(MTT) assay. Culture extract of No.2303 showed cytotoxic effect and induced DNA fragmentation which is hallmark of apoptosis in Colo320 DM and HSR cells. To elucidate the action mechanism of culture extract of No.2303, the effect of messengers of polypeptide growth factors on the anticaner activity of No.2303 was investigated. 12-0-tetradecanoyl phorbol-13-acetate(TPA) and dibutyryl cAMP(db cAMP) plus 3-isobutyl-1-methylxanthine(IBMX) were used as second messengers of growth factor because they act like diaclyglycerol(DAG) and cAMP respectivly inside of the cells. Anticancer activity and apoptosis induced by No.2303 were inhibited by treatment with TPA and db cAMP plus IBMX. These results imply that No.2303 induce anticancer activity by inhibiting c-myc expression and inducing apoptosis through pathway which involves the activation of protein kinase C(PKC) and protein kinase A.

세포질의 알카리화가 Fibroblast Monolayer에 미치는 Mitogenic Effect

文順玉 水原大學校 1989 論文集 Vol.7 No.-

Stimulation of ?? exchange by growth factors has been implicated as an event required for progression of cells from quiescence to a state competent for proliferation. To test this hypothesis, confluent monolayers of mouse and human fibroblasts, were treated with the carboxylic ionophore monensin which catalyzes ?? exchange at the plasma membrane. When ?? monensin is incubated with cells DNA synthesis is stimulated. Growth factors that stimulate ?? exchange also induce expression of two cellular proto-oncogenes, c-fos and c-myc. These gene products have been implicated as regulators of proliferation of mammalian cells. Transient exposure of quiescent Balb/c-3T3 cells to monensin stimulates expression of both c-fos and c-myc proto-oncogene. These data suggest that stimulation of ?? exchange regulates the level of mRNA decoding for c-fos and c-myc and provide a key stimulus for cell proliferation.

Polypeptide 성장인자의 second messenger가 c-fos 및 c-myc 발현에 미치는 영향

文順玉,金鉉培 수원대학교 산업기술연구소 1992 산업기술연구소논문집 Vol.7 No.-

Various polypeptide growth factors stimulate the expression of c-fos and c-myc genes. To elucidate the mechanism by which growth factors stimulate these genes we investigate the effect of agents which mimic second messengers on the expression of c-fos and c-myc proto-oncogene. TPA and A23187 increased the amount of the e-fos and c-myc mRNA respectively and also showed synergistic effect in the expression of c-fos. The effect of TPA and IBMX on the expression of c-fos was different from that of c-myc.

가용성 난각막이 Fibroblast 세포의 증식 및 Type Ⅲ Collagen 생성에 미치는 영향

문순옥 수원대학교 기초과학연구소 1996 基礎科學論文集 Vol.5 No.-

In this study it has been demonstrated that egg membrane hydrolysate(EMH) is able to stimulate the proliferation of NIH 3T3 cells and increase the production of type Ⅲ collagen in NIH 3T3 and Malme-3 (human skin fibroblast) cells. The addition of EMH to quiescent NIH 3T3 cells resulted in an increase of proliferation which was assayed by MTT method. The maximum effect of EMH was detected in 0.4% EMH treated cells which was comparable to that of 5% serum (96% of 5% serum effect). The addition of EMH to NIH 3T3 and Malme-3 cells also increased the production of type Ⅲ collagen in both cells. These results indicate that EMH may enhance the repair process after injury and prevent aging processes in connective tissues.

비안와대뇌형 모균증 치험 3례

문영일,김종남,홍순관,이지애,정옥경,김문정 梨花女子大學校 醫科大學 醫科學硏究所 1997 EMJ (Ewha medical journal) Vol.20 No.3

Rhinocerebral mucormycosis is a well-described fulminant fungal infection that typically presents in a rapidly fulminant manner. This condition is more likely to occur among patient with diabetes mellitus, malignant tumors, who have long-term steroid treatment, or who suffer from some debilitating diseases. We report 3 cases of rhinoorbitocerebral mucormycosis, all suffered from diabetes mellitus. In all 3 cases, endoscopic sinus surgery was done and the antifungal agent(amphotericin B) was administered intravenously, and two patients died. One survivor who had focal extension of paranasal sinus and orbit, required one surgical intervention without orbital exenteration and he is alive without recurrence disease. The severity of the disease is probably dependent upon the gost resistance to the invasion of phycomycetes. Early diagnosis and immediate, effective treatment are the key for cure of the disease.

OCT 플라스미드를 갖는 원유 분해세균에 의한 Octane 분해능

최순영,김창숙,이명혜,황문옥,민경희 숙명여자대학교 자연과학연구소 1991 자연과학논문집 Vol.- No.2

원유 분해세균에 의한 원유 분해능을 조사한 결과, Xanthomonas campestris M12. Xanthomonas sp. M28, Acinetobacter lwoffi G1, and Klebsiella pneumoniae L25, 그리고 Pseudomonas maltophilia N246 등의 순서로 나타났다. Xanthomonas campestris M12, Xanthomonas sp. M28, 그리고 Pseudomonas maltophilia N246 균주 모두 octane 분해시의 온도는 30℃에서 최적이었으며, 최적 pH는 X. campestris M12와 Xanthomonas sp. M28이 7.0∼7.5이었고, P. maltophilia N246이 7.5∼9.0이었다. N246 균주의 최적 NaCl 농도는 3.0∼3.5%이었다. P. maltophilia N246와 X. campestris M12는 모두 플라스미드를 갖고 있음을 확인하였고, N246 균주로부터 플라스미드를 제거하였을 경우 octane 분해능이 소실되었으므로 이 플라스미드 위에 octane 분해 유전자가 있음이 확인되었다. 이 균주의 OCT 플라스미드의 크기는 118kb이었다. 또한, N246 균주는 ampicillin 항생제에 내성을 나타내었다. Xanthomonas campestris M12. Xanthomonas sp. M28. Acinetobacter lwoffi G1, and Klebsiella pneumoniae L25, Pseudomonas maltophilia N246 were screened to increase the ability for crude oil utilization. All of these could utilize hexadecane and octane with the exception of N 246 strain for only octane biodegradation. Thus N246, M12, and M28, strains were specially examined for octane oxidation. Octane biodegradation by three strains showed the optimal conditions at 30C, pH7.0∼9.0 and 0.2∼0.3% octane concentration as a substrate. It was found that P. maltophila N246 and X. campestris M12 had plasmid and the cured plasmid from N246 strain lost octane utilization. Therefore, it was confirmed that certain genes for octane utilization were located on OCT plasmid in N246 strain. The size of OCT plasmid in N246 strain was 118 kb. The N246 strain was resistant to ampicillin.

뇌졸중환자의 사회적 재적응과 관련요인

조남옥,서문자,김금순,김인자,최희정,정성희 성인간호학회 2001 성인간호학회지 Vol.13 No.1

Social readjustment is very important in rehabilitation of stroke survivors. The purpose of this study was to examine the level of social readjustment and related factors in stroke survivors. Especially, to find the most useful predictor for social readjustment is a major point of this research. The study included 254 outpatients who were given follow-up care after discharge from. Occupational rehabilitation and resumption of the perceived meaningful social activity prios to the stroke were used to measure social readjustment. The resumptions of first meaningful social activity was the best predictor for life satisfaction in stroke survivors(r=.245, p<.01). The substance of perceived meaningful social activities were job, meeting with friends, hobby and activities for the family. The sum of meaningful social activities (r = 175. p<.01) and occupational rehabilitation (r= 116, p<.05) were significantly related to life satisfaction. There were significant difference in IADL, depression and life satisfaction according to resumption of meaningful social activity. But, occupational rehabilitation was not related to depression. The level of social readjustment was different between occupational rehabilitation and resumption of meaningful social activity. It was 45.7% in the first meaningful social activity and 36.6% in occupational rehabilitation. The related factors with resumption of the first meaningful social activity were that of IADL, depression, illness intrusiveness and cognitive function. And the level of IADL, illness intrusiveness, cognitive function, and age were significantly related to occupational rehabilitation. In conclusion, we suggest that the resumption of the first meaningful activity in stroke survivors is the best predictor of social rehabilitation. Thus. nurses need to work for resumption of meaningful activity as well as occupational rehabilitation.

성인간호학회지 논문(1989-2000)의 내용과 경향 분석

서문자,김소선,송미순,이명선,오의금,신경림,김옥수,이숙자,이영자,오효숙,정재심,김철규 성인간호학회 2001 성인간호학회지 Vol.13 No.4

"Journal of Korean Academy of Adult Nursing" has been published since 1989 and more than 330 articles have been published up 2000. We reviewed all the articles published in this journal to analyze the contents and trend of published articles and to find out the way of improvement both in quantity and quality of studies in this area. The results are as follows : 1) Ninety percent of articles were quantitative studies. Experimental design was used in 16.1% and quasi-experimental design was adapted in 73.6% of articles. Qualitative research were 10.3% of total articles reviewed. 2) Only 5.1% of articles described theoretical framework and 73.6% of experimentally designed articles included research hypotheses. 3) Among experimental studies, only one was true experimental studie design. Eighty nine point three persent had descriptive survey method among non-experimental studies. Amog the thirty four qualitative studies, phenomenologic(73.5%), grounded theory(14.7%), and ethnographic (11.8%) design were used. 4) Most of research subject were patients and few healthy people, healthcare worker, and patient family. 5) Psychosocial data collection method which used questionnaire were 53.7% and interview. physical measurement, observation were used in 22.8%. 8.2% 5.4% of articles respectively. 6) Total number of keywords were 692, and average number of key words per article was 2.2. Health-related keyword was the most frequently used according to four metaparadigm of nursing (human, health, nursing, and environment). Environment related keyword was rarely appeared. The number and quality of nursing research in "Journal of Korean Academy of Adult Nursing" have been improved continuously. It is difficult to find out special trend or characteristics which is unique in this journal because of relatively short history of adult nursing studies. This review study was performed to analyze adult nursing studies only in the view of quantitative aspect, but it is needed to analyse qualitatively to find philosophical or theoretical trend in nursing.