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국내 분리 Bacillus anthracics의 Plasmid pXO1과 pXO2의 유전적 다양성 분석
문성희,유천권,오희복,성원근,전정훈,유재연,이상섭,Mun, Sung-Hee,Yoo, Cheon-Kwon,Oh, Hee-Bok,Seong, Won-Keun,Chun, Jeong-Hoon,Yu, Jae-Yon,Lee, Sang-Seob 대한미생물학회 2003 Journal of Bacteriology and Virology Vol.33 No.4
We compared genetic variations in virulence mega plasmids pXO1 and pXO2 of twenty-seven Bacillus anthracis strains from Korean patients and environmental samples together with those of Bacillus anthracis Sterne, Pasteur and A2012 standard strains. Genetic variations were analyzed in twenty-three variable regions (ten and thirteen variable-number tandem repeats and insertion/deletions in pXOl and pXO2, respectively). The pXO1 plasmids were classified into 7 groups and pXO2 plasmids to 12 groups. Discrete phylogenic lineages could be differentiated between environmental and clinical strains by UPGMA (unweighted pair group method with average) method. In addition, clinical strains showed more variations than environmental isolates. The pXO2 plasmid appeared genetically more unstable than pXO1. A general plasmid genotype could be suggested for Korean soil isolates since they mostly clustered into a representative group.
2016년 소양호에서 채집한 자연산 잉어과 어류와 갑각류의 법정전염병 및 기생충성 질병 모니터링
문성희 ( Seong Hee Mun ),허준욱 ( Jun Wook Hur ),차승주 ( Seung Joo Cha ),황성돈 ( Seong Don Hwang ),손맹현 ( Maeng-hyun Son ),권준영 ( Joon Yeong Kwon ),권세련 ( Se Ryun Kwon ) 한국수산과학회(구 한국수산학회) 2018 한국수산과학회지 Vol.51 No.1
The presence of pathogens in wild fish is a potential threat to the fish being raised nearby fish farm. Surveillance of these pathogens in the wild is, thus, highly important to keep fish in the farm safe from serious communicable diseases. Fish and crustacean were sampled 4 times at Soyang Lake in 2016. Pathogens for five reportable communicable diseases including KHVD (koi herpesvirus disease), SVC (spring viraemia of carp), EUS (epizootic ulcerative syndrome) and WSD (white spot disease), and parasites were investigated. In Soyang Lake, pale chub Zacco platypus was the most abundant. Forty seven pale chub, 8 Korea piscivorous chub Opsarichthys uncirostris, 3 oily shiner Sarcocheilichthys variegatus wakiyae and 2 crucian carp Cyprinus carassius were investigated for detection of target diseases of fish. Seventeen Caridina denticulata denticulata, 12 Oriental river prawn Macrobrachium nipponense and 1 Chinese mitten crab Eriocheir sinensis were used for detection of WSD. As the result, reportable communicable diseases were not detected in all the freshwater animals. Dactylogyrus was detected in Korea piscivorous chub. Copepoda was also detected in pale chub and Korea piscivorous chub. Metacercaria was detected in the pale chub sampled in September and October. Those were not metacercaria of liver fluke Clonorchis sinensis.
붉바리(Epinephelus akaara) 기형 발생 치어의 형태형성 유전자 발현
유진호,문성희,오현지,백혜자,이영돈,이치훈,권준영,You, Jin Ho,Mun, Seong Hee,Oh, Hyeon Ji,Baek, Hea Ja,Lee, Young-Don,Lee, Chi Hoon,Kwon, Joon Yeong 한국해양생명과학회 2019 한국해양생명과학회지 Vol.4 No.1
붉바리(Epinephelus akaara) 종자생산 시 기형 발생에 의한 손실이 크지만 기형어에 대한 생물학적 정보는 많지 않다. 본 연구에서는 부화 후 96일 붉바리 치어를 정상 그룹과 두 유형의 기형 그룹(머리, 턱)으로 나누어 형태형성과 연관된 4개의 주요 유전자(insulin like growth factor 1: IGF-1, bone morphogenic protein 4: BMP4, peroxisome proliferator-activated receptors γ: PPARγ, matrix Gla protein: MGP) 발현을 조사하였다. 각 그룹에서 뇌, 간 및 근육을 잘라낸 다음 total RNA를 추출한 후 real-time PCR을 사용하여 유전자 발현 차이를 비교하였다(n=20). 부화 후 96일 붉바리 치어에서 IGF-1과 BMP4 유전자는 기형 그룹의 뇌와 간에서 정상 그룹과 비교하여 유의한 발현 차이를 나타냈다(p<0.05). 반면에 PPARγ와 MGP 유전자는 어떤 조직에서도 정상 그룹과 기형 그룹 사이에 유의한 발현 차이를 보이지 않았다. IGF-1과 BMP4 유전자는 치어 단계의 붉바리 기형 상태와 관련되어 있는 것으로 보인다. The deformity occurring at the early developmental stage of red spotted grouper (Epinephelus akaara) causes detrimental effects on the process of juvenile production. In this study, we have compared the expressions of several key genes (insulin like growth factor 1: IGF-1, bone morphogenic protein 4: BMP4, peroxisome proliferator-activated receptors γ: PPARγ, matrix Gla protein: MGP) for morphogenesis between normal and 2 types (cephalic and jaw) of deformed juvenile fish. Expression of these genes were investigated in the brain, liver and muscle of each group of fish (n=20) by real-time PCR. Expression of IGF-1 and BMP4 mRNA in the brain and liver showed significant difference between normal and deformed fish (p<0.05). However, no difference was observed in the expression of PPARγ and MGP mRNA between normal and deformed fish in any tissues. It seems certain that IGF-1 and BMP4 are associated with the state of deformity in juvenile red spotted grouper.
코로나 방전 플라즈마 처리수에 의한 어류 병원체 소독 효과
유진호 ( Jin Ho You ),이지현 ( Ji Hyun Lee ),문성희 ( Seong Hee Mun ),권세련 ( Se Ryun Kwon ),박태섭 ( Tae Sup Park ),권준영 ( Joon Yeong Kwon ) 한국어병학회 2020 한국어병학회지 Vol.33 No.1
Fish culture is constantly threatened by various infectious diseases which are largely transmitted by water. Plasma technology is being used to sterilize polluted water in many industries. In this study, two bacterial pathogens Aeromonas salmonicida and Streptococcus iniae, and a virus (viral hemorrhagic septicemia virus, VHSV) were subjected to plasma water that was produced by a corona discharge system. Growth of A. salmonicida was greatly inhibited from 10<sup>5.61</sup> CFU/ml in positive control to 10<sup>3.51</sup> CFU/ml in treated group by only 60 sec contact with plasma water. Similarly, S. iniae was inhibited from 10<sup>5.85</sup> CFU/ml to 10<sup>3.40</sup> CFU/ml. VHSV titer also decreased from 10<sup>4.1</sup> TCID<sub>50</sub>/ ml to 10<sup>1.45</sup> TCID<sub>50</sub>/ml by the same treatment. Activation of water by the plasma was confirmed by the existence of ozone in the plasma water. These results suggest that plasma water could efficiently disinfect fish pathogens, possibly by the action of reactive oxygen species contained in the plasma water.