겨우살이 lectin의 흰쥐 간세포 독성에 미치는 영향

장철수 ; 류재기 ; 김태호 김천대학교 2007 김천대학교 논문집 Vol.28 No.-

In the present study, we investigated to the cytotoxocity of Viscum album lectin in cultured rat hepatocytes. The cytotoxcity effect in Viscum album lectin was determined by the activity of LDH and the staining of hepatocyte. Viscum album lectin significantly increased LDH, which leakage into medium, of hepatocytes treated or untreated with CCI₄ (P<0.001). However, Viscum album lectin signifi-cantly increased LDH leakage from CC1₄-induced hepatocyte (P<0.001). There was a significant increase in LDH levels relative to the control group. Histological observation basically supportedly the result obtained from LDH assay. The livers of rats challenged with CC1₄ produced a marked increased cyto- plasmic vacuoles and inflammatorycells, while the number of necrotic cells and swollen hepatocytes was not changed significnatly. Rats administered DMSO alone did not alter the normal hepatic architecture. Histological observation of liver sec- tion in rat treated 72 h with either Viscum album lectin CCI₄-induced liver damage showed number of cytoplasmic vaculoe and necrotic cell. The number of inflam- matory cell increased markedly. This results suggest to the conclusion that Viscum album lectin has the effect of hepatotoxicity activator.

면역검사실에서 이월오염에 대한 영향의 분석

류재기 ; 장철수 김천대학교 2008 김천대학교 논문집 Vol.29 No.-

The purpose of the carry-over recovery experiment in clinical immunology test is performed to estimate effect variation from high concentration to low concentration. In practice, carry over experiment is a problem only for analyte with a wide clinical range of analyte concentration, such that a minute degree of carry over could have significant clinical implications. One suggested method to study carry-over contamination is to run known high level patient samples, followed by known low level patient samples to see if the results of the low level material are affected significantly. If carry-over is detected, th team leader and laboratory physician in managing it the analyte concentration above which subsequent patient samples may be affected, and define this output value in the procedure. Carry-over experiment should be expressed as a percentage because the experimental objective is to estimate to reduce effect variation from high level patient sample to low level patient sample, which is a percentage type of error, Good carry-over is 0%. The results of carry-over experiment were Imunoglobulin G (-0.41%), Imunoglobulin A (-0.86%), third complement component (-0.47%) , fourth complement (0.19%) with turbidimetric immunoassay of DADE Behring . hepatitis B surface antibody (0%), hepatitis B surface antigen (-0.004%), hepatitis B core antibody imunoglobulin M (0.12%), hepatitis B core antibody (0%), hepatitis B envelope antigen (0.0043%), hepatitis B envelope antibody (0.012%), with chemiluminescent microparticle immunoassay (CMIA) of ABBOTT Diagnostic Division in Germany. We concluded that the results of carry-over experiment compared to the range of error allowable for the test. There were no item betond the 1% criterion for acceptable performance.

미국토목학회 1989년도 연차 총회 참석후기

劉哲秀(유철수) 대한토목학회 1989 대한토목학회지 Vol.37 No.6

한국인에서 혈청 중성지방, 복부비만과 허리둘레의 상관성연구

장철수 ; 류재기 ; 박갑태 김천대학교 2015 김천대학교 논문집 Vol.36 No.-

Purpose: The purpose of this study was to determine the association between serum triglyceride, abdominal obesity, and waist circumference in Korean. Method: A total of 168 healthy examinees that enrolled in this study were examined at Gimcheon Medical Center from July 2012 to December 2013. Waist circumference (WC) and body mass index (BMI) of subjects were measured as well as blood pressure, fasting blood sugar level, High Density Lipoprotein (HDL-cholesterol), Low Density Lipoprotein (LDL-cholesterol), cholesterol, and C-reactive protein (CRP). Results: In men, WC(p<0.011) had a significant correlation with triglyceride and BMI(p<0.001) had a significant correlation with triglyceride. In women(p<0.001), WC and BMI had a significant correlation with triglyceride. In men, triglycerides (r=0.501, p<0.011) had a positive correlation with WC. However, CRP (r=0.407, p<0.001) had a positive correlation with BMI. In women, triglycerides (r=0.470, p<0.001) and CRP (r=0.367, p<0.001) had a positive correlation with WC. Conclusion: Therefore, WC and triglyceride may be the most effective anthropometric indices for screening Korean people in regards to obesity.

1988년도 대한토목학회 학술발표회 개최보고

劉哲秀(유철수) 대한토목학회 1988 대한토목학회지 Vol.36 No.6

대학생들의 콘텍트렌즈 저장용기의 미생물 오염도 조사

류재기, 장철수, 김현경 김천대학교 2013 김천대학교 논문집 Vol.33 No.-

The use of contact lenses is currently increasing because of a various consumers requirements such as those related to treatment and cosmetic issues. The increase in the number of contact lens users has been accompanied by eye infection due to improper han-dling of contact lenses. Therefore, this study was performed to determine the contamina-tion levels of lens storage cases, which can be an important cause of contamination of con-tact lenses, the cases belonged to students from the Department of Biomedical LaboratoryScience, Gimcheon University. A total of 62 lens storage cases were used in this study, and our results showed that 63% (39 cases) were contaminated with microorganisms. Stenotrophomonas maltophilia, Serratia marcescens, Achromobacter denitrificans, and fungi were detected in 8%, 10%, 72%, and 10%, respectively, of the cases. Stenotrophomonas maltophilia is a non-pathogenic bacterium; however, it is resistant to several antibiotics and is thought to cause endocarditis and meningitis. Eye infection with Serratia marcescens can lead to microbial keratitis and contact lens-induced acute red eye, particularly during overnight contact lens wear. Fungal eye infection is characterized by eye redness, pain, excessive tears formation and blurred vision. In conclusion, the results, which showed that a high proportion of the contact lens storage cases studied were contaminated with bacteria and fungi, suggest that providing proper education regarding the use and storage of contact lenses is important to prevent contact lens-induced eye infection.

유전 알고리즘을 이용한 최적 모델 추종형 보일러-터빈 제어 시스템의 설계에 관한 연구

류철수(C. S. Ryu),황현준(H. J. Hwang),김동완(D. W. Kim),박준호(J. H. Park),황창선(C. S. Hwang) 대한전기학회 1997 대한전기학회 학술대회 논문집 Vol.1997 No.7

메이크업 시 사용되는 퍼프의 사용기간에 따른 오염도에 관한 연구

류재기 ; 김은주 ; 장철수 김천대학교 2010 김천대학교 논문집 Vol.31 No.-

The purposes of this study are to investigate the level of bacteriological contamination of puffs which are used most frequently for touch ups during the day as a makeup tool, and to compare the degree of contamination before and after washing puffs with a neutral detergent. A total of 40 powder puffs that were used for six months to three years have been collected since May, 2005. The collected puff samples were cut into the size of lem by a sterilized scissor, and were put into a sterilized test tube that contained 5ml of DW (distilled water). Then the tube was strongly agitated. Supernatants (100ml) con-taining bacteria was applied to TSA (Trypticase Soy Agar), MacConkey agar, and SDA (Sabouraud Dextrose Agar) plates, respectively. The TSA (Trypticase Soy Agar) and MacConkey agar plates were incubated at 37C for 24-48 hours. The SDA (Sabouraud Dextrose Agar) plates were incubated at 28 C for 7-14 days. As a result of the level of contamination of puffs used over a certain period of time, there was no significant difference cxcept aerobic bacteria. Staphylococcus was not detected. Aerobic bacteria was detected from the puffs older than one year and the colony count increased sharply. The puffs used for more than two years had three times more colony count than the one-year old puffs. In particular, out of 10 puffs older than two years, two were found with 500CFU/ml exceeding the limit of microbiology of cos-metics. Also, the puffs older than two years had 31,1% less microbiology after washing with a PH neutral detergent than before washing. Therefore, as the period of using puffs is longer, thorough checks of their hygiene are needed; as the number of using times increase or the length of using times is longer, frequent washing is inevitable. Since this study restrained the number of puffs to 40,7 would be problematic for generalization and a secondary experiment would be necessary for precise identification of cultured germs.

요로감염의 원인균과 항생제에 대한 고찰

조기창 ; 류재기 ; 장철수 김천대학교 2007 김천대학교 논문집 Vol.28 No.-

In order to provide useful information on the choice of adequate drugs in the treatment of urinary tract infections (UTI), this paper analyzed the causative organisms of urinary tract infection and studied their changes in antibiotic sensi- tivity from the year of 2000 to 2004 for five years. 5,452 uropathogens and their antimicrobial sensitivity were analyzed from 4,815 inpatients and outpatients, all of whom had causative organisms more than 105 CFU/ml in their urine cultures. As a result of the study, the incidence of UTI increased.with age and was highest in patients who were in their 70s (21.3%). The incidence of Gram negative organ- isms that were measured through Gram stain decreased from 74.2% in 2000 to 58.7% in 2004, whereas Gram positive organisms increased from 25.8% in 2000 to 41.3% in 2004. The major Gram negative and positive pathogens were Escherichia coli (29.0%), Enterococcus (15.9%), Klebsiella (9.4%), and Escherichia coli was the most importanat pathogen of UTI during the research period. In terms of a test conducted on antibiotic sensitivity to Gram negative organisms, the susceptibility of amikacin, an aminoglycoside, increased from 78.2% in 2000 to 82.3% in 2004, and that of trimethoprim-sulfamethoxazole decreased from 66.2% in 2001 to 60.6% in 2004. As for antibiotic sensitivity to Gram positive organisms, the susceptibility of clindamycin increased from 46.2% in 2000 to 68.2% in 2004. However, the antibiotic susceptibility of trimethoprim-sulfamethoxazolen decreased from 90.7% in 2001 to 76.8% in 2004. As for Escherichia coli, the main causal microorganisms of urinary tract infection, the antibiotic sensitivity of cefa- zolin decreased from 88.3% in 2002 to 83.0% in 2004; as for Klebsiella, the sen- sitivity of amikacin decreased from 93.1% in 2000 to73.7% in 2004; and as for the trimethoprim-sulfamethoxazole, the antibiotic sensitivity of Staphylococcus aureus decreased from 100% in 2001 to 86.7% in 2004. In conclusion, the study confirms that it is possible to use cephalosporins and aminoglycosides as initial experiential antibiotics for urinary tract infection, and suggests that trimethoprim-sulfamethoxazole, traditionally used as primary treat- ment for urinary tract infection, should be used more carefully to treat urinary tract infection accurately.

혈액배양에서 분리된 Extended Spectrum β-Lactamase 생성균의 역학적 특성 조사

서충원 ; 류재기 ; 장철수 김천대학교 2008 김천대학교 논문집 Vol.29 No.-

the data which analyze the results of blood cultures are crucial basic information of the empirical therapy for patients with infection since the patterns of the species of microorganism isolated from blood and the results of antibiotics resistance of in-patients as well as out-patients in one hospital in the middle area of South Korea from 2004 to 2006. Blood culture was performed with BacT/Alert 3D(North Carolina, Durham, USA) for 5 days using BacT/Alert SA (Areobic) and BacT/Alert SN (Aneorbic) medium for adult patient and BacT/Alert PF medium for children patients. The antibiotic susceptibility test was performed with VITEK (BioMerieux vitek. Hazelwood. Missori. USA) automated identification as well as susceptibility equipments. Total 39,305 cases of blood culture sample were investigated and positive patients of 2,216 (20.0%) were found. Among those, there were 40 patient with ESBL positive, and blood culture positive smples were 4,798 (12.2%). ESBL positive bacteria were found in 86 samples (including double checked culture bacteria). The majority of ESBL producing bacteria were E. coli, K. pneumoniae and K. oxitoca as ordering based on the number. The research showed the results that there were monre females than male with the bacterias, more E. coli in over 50 years old aged group than other bacterias and largest number of patients with 13 patient (32.5%) in Chungcheongnam – do province were found. he most common ESBL producing bacteria were E. coli throughout 3 years, but K. pneumoniae and K. ocitoca were also fairly found. Interestingly, E. coli was highly found in over 50 years old patients. In conclusion, recovery progress of patients will be badly affected by the high emergence rate of ESBL producing bacteria due to the using of broad spectrum antibiotics. Therefore, significant considering is necessary in the antibiotic use, and continuous infection control and trace observations for all lpatients in hospitals including infected patients are required.