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      저자 : 장철수 ; 류재기 ; 김태호 (검색결과 2 건)
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      • 겨우살이 lectin의 흰쥐 간세포 독성에 미치는 영향

        장철수 ; 류재기 ; 김태호 김천대학교 2007 김천대학교 논문집 Vol.28 No.-

        In the present study, we investigated to the cytotoxocity of Viscum album lectin in cultured rat hepatocytes. The cytotoxcity effect in Viscum album lectin was determined by the activity of LDH and the staining of hepatocyte. Viscum album lectin significantly increased LDH, which leakage into medium, of hepatocytes treated or untreated with CCI₄ (P<0.001). However, Viscum album lectin signifi-cantly increased LDH leakage from CC1₄-induced hepatocyte (P<0.001). There was a significant increase in LDH levels relative to the control group. Histological observation basically supportedly the result obtained from LDH assay. The livers of rats challenged with CC1₄ produced a marked increased cyto- plasmic vacuoles and inflammatorycells, while the number of necrotic cells and swollen hepatocytes was not changed significnatly. Rats administered DMSO alone did not alter the normal hepatic architecture. Histological observation of liver sec- tion in rat treated 72 h with either Viscum album lectin CCI₄-induced liver damage showed number of cytoplasmic vaculoe and necrotic cell. The number of inflam- matory cell increased markedly. This results suggest to the conclusion that Viscum album lectin has the effect of hepatotoxicity activator.

      • 겨우살이 lectin의 흰쥐 간세포 독성에 미치는 영향

        장철수 ( Choul Soo Chang ),류재기 ( Jae Ki Ryu ),김태호 ( Tae Ho Kim ) 대한임상검사과학회 2007 대한임상검사과학회지(KJCLS) Vol.39 No.3

        In the present study, the author investigated to the cytotoxocity in cultured rat hepatocytes of Viscum album lectin. The cytotoxcity effect in Viscum album lectin on the activity of LDH was also investigated. Viscum album lectin significantly increased LDH leakage into medium of hepatocytes treated or untreated with CCl4 (p<0.001). However, Viscum album lectin significantly increased LDH leakage from CCl4-induced hepatocyte (p<0.001). There was a significant increase in LDH levels relative to the control group. Histological observation basically supported the result obtained from LDH assay. The livers of rats challenged with CCl4 produced a marked increased cytoplasmic vacuoles and inflammatory cells in number, while the number of necrotic cells and swollen hepatocytes did not change significnatly. Rats administered DMSO alone did not alter the normal hepatic architecture. Histological observation of liver section in rat treated 72 hrs with either Viscum album lectin CCl4-induced liver damage showed number of cytoplasmic vaculoe and necrotic cell. The number of inflammatory cell increased markedly. This results suggest to the conclusion that Viscum album lectin has a effect of hepatotoxicity activator.

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