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The purposes of this study are to investigate the level of bacteriological contamination of puffs which are used most frequently for touch ups during the day as a makeup tool, and to compare the degree of contamination before and after washing puffs with a neutral detergent. A total of 40 powder puffs that were used for six months to three years have been collected since May, 2005. The collected puff samples were cut into the size of lem by a sterilized scissor, and were put into a sterilized test tube that contained 5ml of DW (distilled water). Then the tube was strongly agitated. Supernatants (100ml) con-taining bacteria was applied to TSA (Trypticase Soy Agar), MacConkey agar, and SDA (Sabouraud Dextrose Agar) plates, respectively. The TSA (Trypticase Soy Agar) and MacConkey agar plates were incubated at 37C for 24-48 hours. The SDA (Sabouraud Dextrose Agar) plates were incubated at 28 C for 7-14 days. As a result of the level of contamination of puffs used over a certain period of time, there was no significant difference cxcept aerobic bacteria. Staphylococcus was not detected. Aerobic bacteria was detected from the puffs older than one year and the colony count increased sharply. The puffs used for more than two years had three times more colony count than the one-year old puffs. In particular, out of 10 puffs older than two years, two were found with 500CFU/ml exceeding the limit of microbiology of cos-metics. Also, the puffs older than two years had 31,1% less microbiology after washing with a PH neutral detergent than before washing. Therefore, as the period of using puffs is longer, thorough checks of their hygiene are needed; as the number of using times increase or the length of using times is longer, frequent washing is inevitable. Since this study restrained the number of puffs to 40,7 would be problematic for generalization and a secondary experiment would be necessary for precise identification of cultured germs.