일반연제 발표 : 불임 ; 체외수정 배양액에 대한 제대혈청 및 단백질 첨가의 배아성장 자극효과에 관한 연구

류무현,임영경,이여일 대한산부인과학회 1991 대한산부인과학회 학술대회 Vol.68 No.-

난소갑상선종 3예

류무현,최호선,변지수 대한산부인과학회 1990 대한산부인과학회 학술대회 Vol.66 No.-

Modified Continuous Wave Technique을 이용한 근관충전시 형성된 치근단 크기가 치근단 폐쇄에 미치는 영향

류무현,정일영,이승종,신수정,김의성 大韓齒科保存學會 2008 Restorative Dentistry & Endodontics Vol.33 No.1

본 연구에서는 근관형성의 치근단 크기가 근관 충전의 치근단 폐쇄에 미치는 영향에 대해 알아보고자 하였다. 36개의 단근관, 단근치를 대상으로 3가지 치근단 크기 (Master apical file : #25, #40, #60)에 따라 3가지 실험군으로 나누어 Profile^(ⓡ) LightSpeed^(ⓡ)를 이용하여 근관을 확대하고, Modified continuous wave technique으로 근관 충전을 시행하였다. 미세누출의 측정을 위해 실험군당 10개의 치아를 1주일간 India ink에 넣어 색소 침투를 일으키고 치아를 투명화시켜 미세누출의 정도를 측정하였다. 미세누출에 치근단 크기가 미치는 영향을 분석하기 위하여 Kuskal-Wallis one-way ANOVA를 시행하였고, 사후 검정을 위하여 Student-Newman-Keuls method를 시행하였다. 또한, 치아 단면의 형태를 관찰하기 위하여 색소 침투까지 실행된 각 실험군당 2개의 치아를 근첨 1 ㎜에서 4 ㎜까지 1 ㎜간격으로 수평 절단하고 사진 촬영하였다. 각 실험군에서 측정된 미세누출양을 Kruskal-Wallis one-way ANOVA에서 분석 한 결과 치근단 크기는 유의성 있게 (P < 0.05) 미세누출에 영향을 미쳤으며 치근단 크기가 클수록 미세누출의 평균값과 표준편차 값은 감소하는 경향을 보였다. Student-Newman-Keuls method를 통한 사후 검정에서 치근단 크기가 #25 - #40, #25 - #60에서 유의한 차이를 보였으며 #40 - #60간에는 통계적인 유의차는 없었다. 단면형태에서는 대부분의 치아에서 난원형의 근관 형태를 관찰 할 수 있었고, 치근단의 크기가 작을수록 성형된 근관에 불규칙한 내면이 존재하였고 gutta-percha나 sealer로 충전되지 않는 recess의 면적 이 증가함을 알 수 있었으며 이러한 recess는 미세누출의 통로가 됨을 확인 할 수 있었다. 본 연구 결과, Modified continuous wave technique으로 근관충전시 근관 형성된 치근단 크기가 커질수록 보다 일관되고 성공적인 결과를 예상할 수 있는 근관충전을 시 행할 수 있음을 알 수 있었다. This study examined the influence of the apical sizes on the sealing ability of a root canal filling. Thirty-six single rooted teeth with a single canal were divided into 3 groups (n = 12) and instrumented with either the Profile^(ⓡ) or LightSpeed^(ⓡ) system to achieve three different apical sizes (master apical file (MAF) of #25, #40, or #60). The teeth were filled with gutta percha using a modified continuous wave technique. The level of microleakage was determined by immersing ten teeth from each group into India ink for 1 week followed by clearing with nitric acid, ethyl-alcohol, and methylsalicy late. The microleakage was measured using vernier calipers. The data was analyzed statistically using Kruskal-Wallis one-way ANOVA and a Student-Newman-Keuls Method. Two teeth from each group were sectioned horizontally at 1, 2, 3 and 4 ㎜ from the apex in order to observe a cross section. The apical size was significantly (p < .05) influenced the level of microleakage. In the Student-Newman-Deuls Method, MAF sizes of #25 and #40; and MAF sizes of #25 and #60, respectively showed a statistically significant difference. There was no significant difference between #40 and #60. In most cross sections, oval-shaped canals were observed, and the irregularity of the internal surface increased with decreasing apical size. There was also an increase in the area of recess, which is the area where the canal space is not filled with either gutta-percha or sealer. When the root canals are filled using a modified continuous wave technique, canal filling with more consistent and predictable outcome may be expected as the apical preparation size is increased.

원발성 간장임신 1 예

이여일,류무현,정상우 대한산부인과학회 1992 Obstetrics & Gynecology Science Vol.35 No.11

Primary hepatic pregnancy is an extremely rare form of extrauterine pregnancy. Only three cases have previously been documented, none of which occured in Korea. A case of this rare entity is presented in which a pregnancy was found to arise from the posteroinferior segment of right liver lobe, requiring segmentectomy. In addition to the classic signs of an ectopic pregnancy, a history of right upper abdominal pain was the only distinguishing feature.

환경문제에 관한 고등학생들의 인식에 관한 연구

손부순,류무현,정용택,문정숙,박태술 한양대학교 환경및산업의학연구소 2001 環境과 産業醫學 Vol.10 No.1

The purpose of this study is to provide the basic data in solving the environmental problem and in environmental education to Senior-highschool's students by searching the consciousness of senior-highschool's students on the environmental problem, and to clarify the effects of the environmental education system. The study is accomplished by the survey which includes a total of 473 students. 1.In the source of information concerning the environment, 69.6% of respondents from the TV, 13.5% of them from the newspaper and 9.3% of them from their schools. 2.Regarding the overall conditions of the environmental pollution in our country, 87.7% of respondents marked "Serious". 3.In suffering from Air pollution, 45.5% of respondents sometimes suffered, 26.0% of them frequently suffered and 14.3% of them always suffered. In suffering from noise pollution, 46.5% of respondents sometimes suffered, 32.6% of them frequently suffered, 14.2% of them always suffered. 4.In the attitude whether they participate in the environmental protection campaign spontaneously, 81.0% of respondents answer affirmatively. 5.Regarding the correlation between the economic progress and environmental protection, 67.6% of respondents are environmental protection.

자궁경부암에서 인형 유두종바이러스 16/18형 감염과 p53 유전자 변형과의 상관성

변지수,이여일,류무현,정상우,남종희,박형기 대한산부인과학회 1997 Obstetrics & Gynecology Science Vol.40 No.2

Recent studies of molecular biology have suggested that infection with human papillomavirus(HPV) is implicated in the pathogenesis of cervical carinoma. HPV infection alone, however, does not appear to be sufficient for the process of maliganant transformation, suggesting the requirement of additional cellular events. The mutation of p53, which is involved in negative control of cell proliferation, may play a role in the carcinogenesis of cervical carcinoma. The present study was designed to clarify the association between infection with HPV and p53 alteration in primary carcinoma of human uterine cervix. We investigated 46 prim-ary cervical carcinomas for the presence of HPV DNA by in situ hybridization(ISH) with probe specific for HPV 16/18, and examined the accumulation of p53 protein by immunohis-tochemistry(IHC) and the p53 alteration by polymerase chain reaction-single strand confor-mation polymorphism(PCR-SSCP) using formalin fixed, paraffin -embedded tissue. HPV DNA 16/18 was detected in 18 cases(39.1%) of 46 cervical carcinomas. The accumulation of p53 was identified in tumor cells: low level 43.5%(20/46) and high level 32.6% difference of positive reaction by IHC method. But there was no statistical significant between the infection of HPV and the accumulation of p53(p=0.847). Mutations in exons 4 through 9, where the vast majority of point mutations were reported in human neoplasms, were screened by PCR-SSCP analysis. Altered mobilities of the PCR product of p53 were also found in 9 cases(26.5%) of 34 cervical carcinoma: one in exon 4, four in exon 5/6, two in exon 7, and two in exon 8/9. The mutation of p53 was observed in 41.1%(19/46) respective of the result of IHC and PCR-SSCP, and there was slightly higher p53 alteration in HPV negative cases(23.8%, 11/46) than in HPV positive cases(17.4%, 8/46) without statistical significance(p=0.729). The conclusion of these observations suggests that HPV infection and alteration of p53 may play a critical role in tumorigenesis of carcinoma of the human uterine cervix independently, ant there is important difference in the tumorigenic pathway between two factors.

체외수정배양액에 대한 제대혈청 및 단백질첨가의 배아성장 자극효과에 관한 연구

이여일,임영경,류무현 대한산부인과학회 1992 Obstetrics & Gynecology Science Vol.35 No.8

단순배양액SECM 과 복합배양액Ham`s F-10, TC199에 생쥐 2세초기 배아를 이용하여 제대혈청 및 BSA를 첨가 배양하고 각각 72시간후의 상실배와 배포까지의 난할율을 관찰하여 다음과 같은 결과를 얻었다. 1. 단순배양액SECM, SECM+HCS(10%) 그리고SECM+BSA(4mg/ml)에서 각각 52.1%, 86%, 79.8%의 난할율을 얻었다. 2. 복합배양액Ham`S F-10, Ham`s F-10 + HCS 그리고 Ham`s F-10+ BSA(4mg/ml)에서 각각 69%, 85.7%, 82.8%의 난할율을 얻었다. 3. 복합배양액TC199+HCS(10%), TC199+ BSA(4mg/ml)에서 각각 86.1%, 87.5%의 난할율을 보였다. 4. 이상과 같은 결과로 미루어 보아 단순배양액과 복합배양액 모두BSA 및 제대혈청 첨가군에서 생쥐 배아발육이 촉진됨을 알 수 있었고, BSA와 제대혈청 첨가군 간의 배아성장 자극효과는 유의있는 차이를 보이지 않았다. Two cell stage mouse embryos were cultured in SECM, SECM with 10% human cord serum (HCS), SECM with 4mg/ml bovine serum albumin (BSA), Ham`s F-10, Ham`s F-10 with 10% HCS, Ham`s F-10 sith 4mg/ml BSA, TC 199 with 10% HCS or TC199 with 4mg/ml BSA. Development was assessed by the percent of embryos developed to the morula and blastocyst. Development to the blastocyst stage was significantly higher in Ham`s F-10 with 10% HCS or 4mg/ml BSA than Ham`s F-10 only and also significantly higher in SECM with 10% HCS or 4mg/ml BSA than SECM only. In our results, human cord serum and protein supplement promote the development of two cell stage mouse embryo to the blastocyst stage.