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RISS 인기검색어
- 검색키워드
- 저자 : 라세둘(M. R. Hoque) (검색결과 4 건)
- 무료
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- 유료
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Identification of SNPs in Highly Variable Lysozyme Gene in Korean Native Chicken Populations
라세둘,강보석,임희경,최강덕,이준헌,Hoque, M.R.,Kang, B.S.,Lim, H.K.,Choi, K.D.,Lee, J.H. Institute of Agricultural Science 2010 Korean Journal of Agricultural Science Vol.37 No.3
닭의 진화를 이해하기 위하여 변이가 많다고 알려진 LYZ 유전자의 엑손과 인트론에 존재하는 단일염기다형이 본 연구를 통해 확인되었다. 2개의 한국 재래실용계에서 총 24개체의 DNA 샘플이 본 연구에서 이용되었으며 단일염기 다형의 확인을 위하여 3개체의 샘플을 혼합하여 염기서열 분석을 실시하였다. 적색야계와의 비교를 통하여 두 한국 재래실용계는 18개의 염기서열변이를 확인할 수 있었으며 한국 재래실용계 간에는 15개의 염기서열 변이를 확인할 수 있었다. 총 33개의 변이 중 두 개의 삽입변이(21 bp와 4 bp)가 확인되었다. 한편, 2번째 엑손의 1426 bp 위치에 존재하는 단일염기 다형(p.Ala49Val)은 아미노산의 변이를 나타내는 미스센스 돌연변이로 확인되었다. 이 돌연변이는 이 lysozyme 효소의 촉매작용을 하는 위치에 놓여 있어 효소의 활성과 밀접한 관계가 있을 것으로 추정된다. 본 연구에서 밝혀진 LYZ 유전자의 변이는 이 유전자의 기능뿐 아니라 한국 재래실용계 집단의 구조를 이해하는데 기초자료로 이용될 것으로 사료된다. Single nucleotide polymorphisms (SNPs) in chicken lysozyme (LYZ) gene were investigated in this study. The identification of SNPs in both exon and intron in LYZ gene has led to understanding of evolution for the domestic chicken populations. A total of 24 samples from two Korean native commercial chicken populations (CCPs) were used for the initial identification of SNPs by mixing three DNA samples for sequencing experiments. By comparing with red jungle fowl (RJF), two commercial chicken populations have 18 common polymorphisms. Between two commercial chicken populations, 15 polymorphisms were identified. Of the 33 polymorphisms identified, two indels (21 and 4 bp) were found. Whereas, only one polymorphism in exon 2 at the bp position 1426 was a non-synonymous substitution (p.Ala49Val), indicating the amino acid changes. The identified non-synonymous substitution (p.Ala49Val) is located close to the catalytic sites of the enzyme, which might affect its activity. In our investigation, the polymorphisms in LYZ gene can provide broad ideas for the variation of Korean native chicken populations from the ancestor of chicken breeds as well as the some biological functions of the LYZ gene.
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SNP typing in Korean Native Chicken Mitochondrial D-loop region for Breed Identification
M. R. Hoque(라세둘),B. S. Kang,H. K. Lim,K. D. Choi,J. H. Lee 한국가금학회 2010 한국가금학회 정기총회 및 학술발표회 Vol.27 No.-
The mitochondrial (mt) DNA D-loop sequences have been widely used for identification of origin of the breeds in animals. In this study, single nucleotide polymorphisms (SNP) from chicken mtDNA were investigated for identifying breed differences between the two commercial Korean native chicken (KNC) breeds. A total of 566 individual birds were used in this study. Of these, 280 mtDNA sequences were obtained from different chicken breeds. The sequence data indicated that there are 14 mtDNA clades from the 27 nucleotide variations. We also investigated whether the D-loop hypervariable region in chicken mtDNA found four specific SNPs can be used for the breed identification. In addition to our previous study, three more SNPs were also included for discrimination of KNC breeds. Using the seven SNPs markers, the probabilities of breed identification in CCP1 and CCP2 were 78.4% and 90.8%, respectively. The results obtained in this study can help for the consumers when they select the KNC meats.
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Identification of SNPs in Highly Variable Lysozyme Gene in Korean Native Chicken Populations
M. R. Hoque(라세둘),B. S. Kang(강보석),H. K. Lim(임희경),K. D. Choi(최강덕),J. H. Lee(이준헌) 충남대학교 농업과학연구소 2010 농업과학연구 Vol.37 No.3
닭의 진화를 이해하기 위하여 변이가 많다고 알려진 LYZ 유전자의 엑손과 인트론에 존재하는 단일염기다형이 본 연구를 통해 확인되었다. 2개의 한국 재래실용계에서 총 24개체의 DNA 샘플이 본 연구에서 이용되었으며 단일염기 다형의 확인을 위하여 3개체의 샘플을 혼합하여 염기서열분석을 실시하였다. 적색야계와의 비교를 통하여 두 한국재래실용계는 18개의 염기서열변이를 확인할 수 있었으며 한국 재래실용계 간에는 15개의 염기서열 변이를 확인할 수 있었다. 총 33개의 변이 중 두 개의 삽입변이(21 bp와 4 bp)가 확인되었다. 한편, 2번째 엑손의 1426 bp 위치에 존재하는 단일염기 다형(p.Ala49Val)은 아미노산의 변이를 나타내는 미스센스 돌연변이로 확인되었다. 이 돌연변이는 이 lysozyme 효소의 촉매작용을 하는 위치에 놓여 있어 효소의 활성과 밀접한 관계가 있을 것으로 추정된다. 본 연구에서 밝혀진 LYZ 유전자의 변이는 이 유전자의 기능뿐 아니라 한국 재래실용계 집단의 구조를 이해하는데 기초자료로 이용될 것으로 사료된다. Single nucleotide polymorphisms (SNPs) in chicken lysozyme (LYZ) gene were investigated in this study. The identification of SNPs in both exon and intron in LYZ gene has led to understanding of evolution for the domestic chicken populations. A total of 24 samples from two Korean native commercial chicken populations (CCPs) were used for the initial identification of SNPs by mixing three DNA samples for sequencing experiments. By comparing with red jungle fowl (RJF), two commercial chicken populations have 18 common polymorphisms. Between two commercial chicken populations, 15 polymorphisms were identified. Of the 33 polymorphisms identified, two indels (21 and 4 bp) were found. Whereas, only one polymorphism in exon 2 at the bp position 1426 was a non-synonymous substitution (p.Ala49Val), indicating the amino acid changes. The identified non-synonymous substitution (p.Ala49Val) is located close to the catalytic sites of the enzyme, which might affect its activity. In our investigation, the polymorphisms in LYZ gene can provide broad ideas for the variation of Korean native chicken populations from the ancestor of chicken breeds as well as the some biological functions of the LYZ gene.
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홀스타인종과 한우에 있어서 BoLA-DRB3 유전자의 단일염기다형과 반수체 분석
정행진(Hang-Jin Jeong),유성란(Seong-Lan Yu),라세둘(M. R. Hoque),이준헌(Jun-Heon Lee),도창희(Chang-Hee Do),류승희(Seung-Heui Ryoo),상병찬(Byung-Chan Sang) 충남대학교 농업과학연구소 2011 농업과학연구 Vol.38 No.1
BoLA (bovine leukocyte antigens) have been known as gene complex related with bovine diseases and immunological traits. This study was conducted to find out the characteristics of BoLA-DRB3 gene related to mastitis and BL(bovine leukocyte) from 280 cattle [193 animals of Holstein cattle and 87 animals of Hanwoo]. As a result, five PCR-RFLP types (b, d, e, f and g) using HaeⅢ restriction enzyme, three BstYI restriction patterns (b, d and e) and eight RsaI restriction types(b, d, f, I, j, n, o and w) were identified. Moreover, we identified new d' type (197→175/22), having one more cutting site by BstYI enzyme than d type allele and n' type (180→169/11) having one more cutting site by RsaI enzyme than n allele was additionally identified. Next, we identified 53 SNPs in BoLA-DRB3 exon2 from 280 cattle. SNP frequency and heterozygosity of Holstein and Hanwoo were investigated in all the SNP genotype. These results might be based on research for identifying marker associated with bovine diseases.
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