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cDNA cloning and red light regulated expression of pea chloroplast fructose - 1,6 - bisphosphatase
동승명,임재환,전용희,한태룡 한국농화학회 1993 Applied Biological Chemistry (Appl Biol Chem) Vol.36 No.3
Chloroplast fructose-1,6-bisphosphatase from pea seedlings was purified to homogeniety. Polyclonal antibody was raised from the purified enzyme and used for cDNA cloning and immunoblot analysis. The amino acid sequence of the coding region of cloned cDNA was 83-85% homologous with that of chloroplast enzyme from wheat and spinach, whereas it was only 43% homologous with that of spinach cytosolic enzyme. The enzyme activity of chloroplast fructose-1,6-bisphosphatase was markedly increased by light: illumination. Immunoblot and northern blot analysis indicate that the expression of the enzyme was stimulated by red light while repressed by far red light, suggesting that the enzyme expression is regulated by phytochrome. The enzyme was maximally expressed in pea leaves but not expressed in stems and roots.
동승명,이현규,조성규,권승현,윤희제,권현진,이지혜,김혜림,박필구,김호근,S. Diane Hayward,박전한,이재면 한국미생물학회 2015 The journal of microbiology Vol.53 No.1
Interferon regulatory factor-5 (IRF-5), a member of the mammalianIRF transcription factor family, is regulated by p53,type I interferon and virus infection. IRF-5 participates invirus-induced TLR-mediated innate immune responses andmay play a role as a tumor suppressor. It was suppressed invarious EBV-infected transformed cells, thus it is valuable toidentify the suppression mechanism. We focused on a promoterCpG islands methylation, a kind of epigenetic regulationin EBV-associated Burkitt’s lymphomas (BLs) and gastriccarcinomas. IRF-5 is not detected in most of EBV-infectedBL cell lines due to hypermethylation of IRF-5 distalpromoter (promoter-A), which was restored by a demethylatingagent, 5-aza-2 -deoxycytidine. Hypomethylation ofCpG islands in promoter-A was observed only in EBV type IIIlatent infected BL cell lines (LCL and Mutu III). Similarly,during EBV infection to Akata-4E3 cells, IRF-5 was observedat early time periods (2 days to 8 weeks), concomitant unmethylationof promoter-A, but suppressed in later infectionperiods as observed in latency I BL cell lines. Moreover, hypermethylationin IRF-5 promoter-A region was also observedin EBV-associated gastric carcinoma (EBVaGC) cell lines orprimary gastric carcinoma tissues, which show type I latentinfection. In summary, IRF-5 is suppressed by hypermethylationof its promoter-A in most of EBV-infected transformedcells, especially BLs and EBVaGC. EBV-induced carcinogenesistakes an advantage of proliferative effects of TLRsignaling, while limiting IRF-5 mediated negative effects inthe establishment of EBVaGCs.
cDNA CLONING AND RED LIGHT REGULATED EXPRESSION OF PEA CHLOROPLAST FRUCTOSE - 1,6 - BISPHOSPHATASE
동승명,임채환,전용희,한태룡 한국응용생명화학회(구 한국농화학회) 1993 한국응용생명화학회 학술발표회 Vol.1993 No.-
Chloroplast fructose-1,6-bisphosphatase from pea seedlings was purified to homogeniety. Polyclonal antibody was raised from the purified enzyme and used for cDNA cloning and immunoblot analysis. The amino acid sequence of the coding region of cloned cDNA was 83-85% homologous with that of chloroplast enzyme from wheat and spinach, whereas it was only 43% homologous with that of spinach cytosolic enzyme. The enzyme activity of chloroplast fructose-1,6-bisphosphatase was markedly increased by light: illumination. Immunoblot and northern blot analysis indicate that the expression of the enzyme was stimulated by red light while repressed by far red light, suggesting that the enzyme expression is regulated by phytochrome. The enzyme was maximally expressed in pea leaves but not expressed in stems and roots.