근로자의 뇨중 상피세포에서 ³²P-postlabeling에 의한 발암물질의 DNA adducts측정방법에 대한 연구

이진헌,노재훈,그린 탈라스카,Lee, Jin Heon,Roh, Jaehoon,Talaska, Glenn 한국산업보건학회 2000 한국산업보건학회지 Vol.10 No.1

Carcinogen-DNA adduct analysis has potential for biomonitoring the earliest effects of exposure to many chemical carcinogens. They are the covalent reaction products of electrophiles and nucleophilic sites on DNA and the initial damage to DNA induced by many carcinogens. So many researchers begin to use them as biomarker for monitoring the earliest exposure of carcinogens and develop the effective analytical techniques about them. Randerath, Gupta and coworkers(1981, 1982) has also developed a $^{32}P$-postlabelling method as one among them. A major project for biomonitoring workers with carcinogen-DNA adducts is to develop non-invasive samples instead of tissues of target organs such as baldder and lung. This study use the exfoliated urothelial cells in urine for examine benzidine-DNA adducts. The content of exfoliated urothelial cells is not enough to significantly measure DNA content with spectrophotometer, and require the another way. So firstly washing the collected cells with PBS and 70% ethanol and centrifuge them for removing the crystals in urine, which block the isolation of DNA adducts. And then, measure the total nucleotide after $^{32}P$-postlabelling for calculating RAL. $[{\gamma}-^{32}P]ATP$ using for $^{32}P$-postlabelling, can synthesize with $[^{32}P]H_3PO_4$, and reagent and enzyme mixture (RM, EM), which is very economic in case of requiring a lot of them. Chromatography was composed of two steps. First step was to separate adduct ones from unadducted nucleotide, and secondary step was separate each adduct, which were performed with 4 kinds of solvents and different directions on TLC. With this procedure, we measure the DNA adducts in exfoliated urothelial cells of workers who were employed in benzidine and benzidine-dye company. RAL of adducts were $89.0{\times}10^7$ and $57.0{\times}10^7$ in them. In conclusion, we can significantly measure the DNA adduct in exfoliated urothelial cells by using the above $^{32}P$-postlabelling procedures, and use them to be biomonitoring workers who exposed carcinogens.

페놀 노출 근로자의 요중 페놀농도에 영향을 미치는 요인

심규진(Gyu Jin Shim),노재훈(Jaehoon Roh),김치년(Chinyon Kim),이신영(Shinyoung Lee),원종욱(Jong Uk Won) 한국실내환경학회 2011 한국실내환경학회지 Vol.8 No.2

Objectives : The purpose of this study is to identify the characteristics that affect the urinary phenol of workers exposed to phenol. Subjects and Methods : Total 41 workers were selected at bisphenol A manufacturing plant and their urinary phenol concentration were measured before and after work along with the phenol concentration in the workplace air, and carried out a survey on work characteristics and lifestyle factors that could affect urinary phenol. Results : The phenol concentration in air during work hours was 0.91 (non-detection~2.88) ㎎/㎥, and the worker’s urinary phenol concentrations before and after work were 100.27±75.76 and 138.13±109. 58 ㎎/g creatinine, respectively, which showed a statistically significant increase. Comparing smoking and urinary phenol concentration, smokers had 194.54±137.52 ㎎/g creatinine while non-smokers had 108.88±80.10 ㎎/g creatinine, thus showing the urinary phenol concentration of smokers to be statistically significantly higher (p=0.046). The urinary phenol concentration increased as work hours, the frequency of skin exposure to phenol, and the amount of drinking increased, and there were differences in its concentration depending on the work type and whether or not workers wore protective gear. The results of carrying out a multiple regression analysis showed that phenol concentration in air, work hours, frequency of skin exposure, and smoking were statistically significant. In other words, the urinal phenol concentration increased more for smokers than for non-smokers, when work hours became longer, and when the frequency of skin exposure was over five times. Conclusions : The factors that influenced urinary phenol in workers exposed to phenol were phenol concentration in air, work hour, frequency of skin exposure, smoking, work and lifestyle habits. Accordingly, biological monitoring for phenol exposure assessment must reflect these factors, and effort must be made to reduce skin exposure at workplace.

이황화탄소를 경구투여한 흰쥐의 혈중 이황화탄소에 관한 연구

조명화(Muyung Hwa Cho),노재훈(Jaehoon Roh),이경종(Kyung Jong Lee),김치년(Chi Nyun Kim),조영봉(Yong Bong Cho),문영한(Young Hahn Moon) 대한직업환경의학회 1993 대한직업환경의학회지 Vol.5 No.2

한 사업장 부속의원의 비용-편익 분석

유인근(Inkeun Yoo),원종욱(Jonguk Won),송재석(Jaeseok Song),노재훈(Jaehoon Roh) 대한직업환경의학회 2001 대한직업환경의학회지 Vol.13 No.2

유증기 회수설비 유무에 따른 주유소 근로자들의 요중 trans, trans-Muconic acid, Hippuric acid에 관한 연구

최재준 ( Jaejun Choi ),원종욱 ( Jong Uk Won ),김치년 ( Chi Nyon Kim ),노재훈 ( Jaehoon Roh ) 한국산업보건학회 2014 한국산업보건학회지 Vol.24 No.2

Objectives: This study aims to investigate the excretion aspect of urinary t, t-MA and hippuric acid by measuring concentrations of urinary metabolites according to the use of gasoline vapor recovery system. Materials:In order to analyze urinary metabolites, samples from the 23 gas station workers ten gas stations in the Seoul and Gyeonggi Province area were collected once daily after work. In addition, a survey was conducted on work factors and lifestyle habits as factors affecting the concentration of urinary metabolites. Results: The average concentrations of t, t-MA and hippuric acid after work were 0.124±0.177 mg/g creatinine and 0.557±0.251 g/g creatinine among workers at gas stations where gasoline vapor recovery systems were installed. The average concentrations of t, t-MA and hippuric acid were 0.160±0.113 mg/g creatinine and 0.682±0.619 g/g creatinine among workes at gas stations where gasoline vapor recovery systems were not installed. Average concentrations were higher at gas stations where a gasoline vapor recovery system was not installed, but the differences were not statistically significant differences. Urinary t, t-MA and hippuric acid average concentrations of smokers and non-smokers were higher in the gas stations where gasoline a vapor recovery system was not installed. T, t-MA as a factor evaluation affecting the concentration of urinary metabolites was not statistically significant in all factors, while hippuric acid was statistically significant only for age(p=0.024). Conclusions: The average concentrations of urinary t, t-MA and hippuric acid were higher in gas stations where gasoline vapor recovery systems were not installed compared to gas stations where such a system was installed. There needs to be an assessment of biological monitoring according to refueling activity considering skin absorption of benzene and toluene and presence of gasoline vapor recovery system.

석유화학공장 대정비 작업 근로자의 벤젠 노출과 요중 trans, trans-muconic acid 배설과의 관계

이승민 ( Seung Min Lee ),원종욱 ( Jong Uk Won ),김치년 ( Chi Nyon Kim ),노재훈 ( Jaehoon Roh ) 한국산업보건학회 2014 한국산업보건학회지 Vol.24 No.1

Objectives: Using laborers participating in the petrochemical factory turnaround process as subjects, this study aims to identify exposure to benzene in the air and examine the relationship between exposure and the excretion of urinary metabolites by measuring concentrations of urinary trans, trans-muconic acid(t,t-MA). Methods: A passive sampler was used to measure the level of benzene in the air. In order to analyze urinary metabolites, the urine of laborers participating in the turnaround process was collected twice daily, both before and after work. In addition, a survey was conducted on work factors and lifestyle habits as factors affecting the concentration of urinary metabolites. Results: During the survey period, benzene was detected in the samples from all workers, and its average concentration was 0.16±0.22 ppm. The average concentration of t,t-MA after work was 1.20±1.86 ㎎/g creatinine, and the results of analyzing urinary metabolites concentration before and after work showed statistically significant differences(p=0.003). There was also a statistically significant correlation(r=0.52, p=0.002) between benzene in the air and the concentration of after-work urinary t,t-MA. Conclusions: During the turnaround process, the average benzene concentration in workers was 0.16±0.22 ppm, which was below the exposure limit. However, their average t,t-MA concentration was 1.20±1.86 ㎎/g creatinine, which exceeded the exposure limit of 1㎎/g creatinine. The characteristics of turnaround process work require considerations such as underestimating the passive sampler being used and the skin absorption of benzene, and there needs to be a simultaneous assessment of working environment measurements and biological monitoring.

사무실 내 라돈 농도에 관한 연구

최현우 ( Hyun Woo Choi ),김치년 ( Chi-nyon Kim ),원종욱 ( Jong-uk Won ),김홍관 ( Hong-kwan Kim ),노재훈 ( Jaehoon Roh ) 한국산업보건학회 (구 한국산업위생학회) 2016 한국산업보건학회지 Vol.26 No.3

Objectives: This study is aimed at examining radon exposure in offices and the factors that can influence the concentrations. Methods: Indoor radon concentrations in a total of 30 places were measured from January 18 to 21, 2016, targeting six buildings in Seoul with different completion years. The measurement was conducted according to the radon measurement guidelines for indoor air suggested by the Ministry of Environment. Results: As a result of comparing each average concentration, underground area concentration was 42.850±22.501 ㏃/㎥, and that of the ground floors was 27.850±12.232 ㏃/㎥, which was lower than the concentration in the underground areas and statistically significant (p=0.045). As a result of comparing the concentration according to whether or not outside air entered, the average concentration for ventilated areas was 24.876±11.833 ㏃/㎥, and the average concentration for enclosed areas was 47.892±19.375 ㏃/㎥. The concentration in ventilated areas was lower at a statistically significant level (p=0.001). Finally, as a result of the multiple regression analysis for evaluating the factors influencing radon concentration, only ventilation was significant (p=0.007). Conclusions: As a result of measuring radon in office buildings, there was no place that exceeding the recommended standard of the US EPA, but the concentration in poorly ventilated areas was measured to be high. An effort to manage radon concentration and reduce it through the improvement of ventilation systems, repeated measurement is necessary in the future.

석유화학 공장 사무실 공기질과 근로자 자각 증상과의 연관성에 관한 연구

김기연(Kiyoun Kim),김혜정(Hyejung Kim),김현수(Hyunsoo Kim),김치년(Chinyon Kim),원종욱(Jonguk Won),노영만(Youngman Roh),노재훈(Jaehoon Roh) 한국실내환경학회 2006 한국실내환경학회지 Vol.3 No.3

This research was performed with the subject of 111 offices in a chemical plant of Korea. Airborne concentration level of carbon dioxide, carbon monoxide, formaldehyde and total suspended particulates in the office rooms were measured along with temperature and relative humidity. Simultaneously, general characteristics and subjective health symptoms of 500 office workers were investigated through a questionnaire consisting of the five point weighting method : 0 = not at all, 1 = a little bit, 2 = moderately, 3 = quite a bit and 4 = extremely. Results showed that the mean values for temperature and relative humidity in investigated office rooms were 22.2℃ and 34.3%, respectively. The concentrations of formaldehyde, PM10, carbon dioxide and carbon monoxide were 0.17 ppb, 28.8 ㎍/㎥, 0.95 ppm and 424 ppm, respectively. 56.4% of all the workers investigated replied with a sense of fatigue or weariness (mean=2.23), drowsiness or languidness (mean=2.22), sneezing (mean=2.11) and ophthalmia oreye fatigue (mean=2.07). Female workers, non-smokers, non-drinkers, workers who do shift more than nine hours a day, and workers in poor health experienced more subjective symptoms than other workers (p<0.05). Subjective symptoms of office workers were significantly associated with the following items of office characteristics: the purchase date of office equipment, the change of office furniture, the use of paint, smoking in the office, ventilation condition, the use of an individual air-conditioner, the use of a fax machine, the degree of office repair, the proportion of workers per office, the degree of satisfaction in office environment, and the operation hours of the air-conditioner. Also, subjective symptoms were positively correlated with indoor environmental factors such as relative humidity, carbon dioxide level, level of PM10, and formaldehyde (p<0.05). In conclusion, office characteristics and air quality in a chemical plant affected subjective health symptoms of office workers. Thus, in order to improve the health of workers, to enhance work efficiency, and to establish a better office environment, air quality control in office rooms by optimal ventilation, adequately occupied number of workers in one office, and maintenance of office equipment should be fulfilled thoroughly.

PET, PET/CT 방사선 종사자의 피폭 관련요인

박훈희(Hoon-Hee Park),이정배(Jeongbae Rhie),정필균(PilkyunJung),이종두(Jong Doo Lee),원종욱(Jong Uk Won),노재훈(Jaehoon Roh) 대한직업환경의학회 2012 대한직업환경의학회지 Vol.24 No.1

염화비닐 노출 근로자에 대한 노출평가 방법에 관한 연구

장정환(Jung Hwan Chang),김치년(Chi Nyon Kim),윤영식(Young Shik Yun),이용호(Yong-Ho Lee),조영봉(Young Bong Cho),노재훈(Jaehoon Roh) 한국항공우주의학협회 2000 항공우주의학회지 Vol.10 No.3