이황화탄소를 경구투여한 흰쥐의 혈중 이황화탄소에 관한 연구

조명화(Muyung Hwa Cho),노재훈(Jaehoon Roh),이경종(Kyung Jong Lee),김치년(Chi Nyun Kim),조영봉(Yong Bong Cho),문영한(Young Hahn Moon) 대한직업환경의학회 1993 대한직업환경의학회지 Vol.5 No.2

이황화탄소를 경구투여한 흰쥐의 혈중 이황화탄소에 관한 연구

조명화,이경종,문영한,조영봉,노재훈,김치년 大韓産業醫學會 1993 대한직업환경의학회지 Vol.5 No.2

Carbon disulfide (CS₂) ditributed in the body by the blood stream, presents free form and bound form carbon disulfide that is acid-labile carbon disulfide(ALCS₂) in blood. ALCS₂could be analyzed by acid-treatment fraction which binds to celluar macromolecules or amino acid. We observed the variation of blood concentration of carbon disulfide and the amounts of free form CS₂and ALCS₂in blood were determined according to their oral doses. After administration for six days, the amount of free form CS₂and ALCS₂in blood was increased significantly through duration of administration for six days. ALCS₂was remained longer than free form CS₂in blood for all doses. The amount of free form CS₂and ALCS₂in red blood cells was larger than that of plasma. As the above results, ALCS₂might be a useful indicator for biological monitoring of CS₂in blood.

흰쥐 적출간 관류법을 이용한 벤지딘계 염료 Direct Black 38의 대사

원종욱,노재훈,김춘성,박윤정,김치년 大韓産業醫學會 1996 대한직업환경의학회지 Vol.8 No.1

Direct Black 38, a kind of benzidine-based azo dye, is widely used as a dye for fabric, leather, cotton, cellulosic material, paper, wool, silk, and so on. Benzidine-based azo dyes are proven as a mutagen and linked to bladder cancer. In 1978, National Institute for Occupational safety and Health recommended that three widely used benzidine-based dyes(Direct Black 38, Direct Blue 6, and Direct Brown 95) should be treated as carcinogens. In this experiment, metabolism of the benzidine-based dye, Direct Black 38 was examined by using an isolated liver perfusion system. To measure the metbolites of Direct Black 38, 8.0μM, 30.5μM and 63,3μM of Direct Black 38 was added into the recirculating perfusate of the isolated perfused rat liver. Samples were collected at 0, 10, 20, 30, 60, 90, 120 minute. They were treated with sep-pak and methanol, and the metabolites were detected and quantified with high performance liquid chromatography(HPLC). Residual non-reactive dye in the perfusate and liver was reduced to benezidine and then analyzed by HPLC. Detected metabolites of Direct Black 38 were benzidine, N-acetybenzidine, and N,N'-diacetylbenzidine. The average concentration of benzidine was 0.1μM and this concentration was maintained throughout the experimental period. The average concentration of Nacetylbenzidine was 0.22μM and took the same pattern of benzidine. When 30.5μM of Direct Black 38 was added to the perfusate, only N,N'-diacetylbenzidine increased slightly with time. From the above results we suggest that only small amount of Dirst Black 38 might be metabolized to benzidine regardless of the s, amount of the Direct Black 38. There are some possible explanations. The liver was damaged during the perparation. And the function of the perfused liver decreased rapidly because adsorbing of dye. The benzidine, acetybezidine and diacetylbenzidine metabolized to other metabolites were not detected in this experiment.

크롬 노출에 의한 건강장해 및 생물학적 지표

노재훈,김치년 가톨릭대학교 산업의학센터 2000 韓國의 産業醫學 Vol.39 No.3

Chromium exposure generally occurs through both environmental and work-related pollution. The exposure limit of chromium compounds should be set differently because the extent of its harmful effects on the body differs depending on its atomic valence and water solubility. The Korean Ministry of Labor classified chromium compounds into simply bichromium and chromic acid. Chromium compounds used as a pigment component are generally insoluble, have strong carcinogenic and toxic effects. Regardless of the large use of chromium compounds in pigment, most health-related interests have extended to plating and welding industries and relatively little to the pigment industry in Korea. Therefore, for proper health management of workers exposed to chromium compounds, including workers involved in pigment manufacturing, the exposure limits should be more specified. The ACGIH uses the urinary chromium concentration as the biological exposure index of manual metal arc welders, who are chronically exposed to chromium. The blood chromium concentration, however, has not been used as an exposure index because of the lack of useful data and analytic difficulties. Presently in Korea biological monitoring of chromium compounds does not account for the different compound types and exposure duration and uses both urinary and blood chromium concentrations selectively. The guidelines of worker's health examination sets the exposure limit of chromium at the end of the workday at 10 μg/g creatinine of urinary chromium, but the ACGIH recommends increasing during shift work at 10μg/g creatinine of urinary chromium. For a more accurate biological monitoring of chromium, more studies on the exposure limits of these agents, and on the revision and supplementation to Occupational Health and Safety Acts are needed.

충남지방의 전통연못유적에 관한 연구 : 예산군 대술면 방산리 내곡지의 현지측량 및 현황조사를 중심으로 Focused on the Excavation Survey on Naegokji in Bangsan - ri , Daesul - myon , Yesan - gun

이재근,김치년 한국정원학회 2001 한국전통조경학회지 Vol.19 No.1

This study is analysis of the traditional pond at Dae-Sul Myeon, Yesan-gun, Chung-Nam based on old documents, actual survey, interviews with inhabitants and Lee's descendents to excavate traditional pond. The important results are following. 1. When Lee San-haes grave was built in mid Yi dynasty, this traditional pond was also built to make a front yard of grave into a very good place by Lee Duck-Hyung (Han Eum, 1561-1613), the second son-in-law of Lee San-hae(1539-1609) on geomancer's advice. 2. Looked down from his grave in Mt. Bonsu whose shape is girl-playing-a string, this artificial pond was not only for getting water but for making a good site with soil in view of feng-shui, Yin and Yang theory. This is a differentiated and specific style compared with those of palaces, high officials gardens, temples and villas. 3. As this pond is in the approach to the town, it comes to play a main entrance role in town and surroundings and its practical usages give inhabitants and Lee's descendents much convenience because it divides rice field into two parts as reservoir, the upper and the lower part. 4. Three round islands in the pond symbolize Bonrae, Bangzang, Youngju and a zelkova tree in each island expresses hermits thought of Taoism pursuing no aging and longevity. We can infer Yin and Yang from the shape of changed trapezoid pond (L: 90M×W: 20M) with round islands in the its middle which was popular in pond near the house at that time. 5. This pond flows from the east-southern corner to north-west coner, and its depth is 1.8M-1M with relatively clean water quality. These mean that heights of both water gate is different in diagonal direction to make its flow smooth. So this pond has kept its good condition in muddy degree, clarity, chromaticity to taking circulation system of traditional ponds. 6. To maintain structural stability, an embankment with small stones are built in lower part, with soil in upper part. But it needs to recover its original shape as a relic because repair work was done with stone for convenience sake in northern part. And two cherry trees and weeping willows around the pond should be repaired into original shapes according to old documents and specialists opinions. This study is about peculiar pond as a relic which was built for another goal and by special method. But due to its rare documents and detail research, this study has lots of limits, and leave them as next tasks.

공기중 염화비닐단량체의 포집시 공기 포집량이 파괴에 미치는 영향

윤존중,임남구,김치년,노재훈 한국산업위생학회 2001 한국산업보건학회지 Vol.11 No.3

The main factors of breakthrough are known to sampling time, flow rate, concentration of the sample, temperature, humidity, and tee physical characteristics of the solid sorbent tube. However, no study has been reported the effect of temperature and sampling volume on the breakthrough of a charcoal tube during vinyl chloride monomer (VCM) sampling. The objective of this study is to suggest the optimal sampling condition during VCM sampling based on National Institute for Occupational Safety and Health(NIOSH) method. To evaluate adequate sampling volume for VCM without breakthrough, volume of 1, 2, 3, 4, and 5 L each from VCM of 1, 5, 10, 15, and 20ppm at flow rate of 0.05 L/min were sampled in 22℃ and 40℃. At 22℃, in the case of 1, 5, 10, and 15ppm, VCM was absorbed completely in first section of charcoal tube regardless of sampling volume. But in 20ppm, detection rates are 99.56℃ in first section and 0.44℃ in second section. At 40℃ of 1ppm. VCM was adsorbed completely in first section. in 10, 15, and 20ppm, detection rates of second, third, and forth sections were decreased significantly by reduction of sampling volume, In determination of breakthrough based on NIOSH method, no breakthrough was occurred in 20ppm at 22℃. At 40℃, breakthrough was occurred in 10, 15, and 20ppm when sampling volume was 5L. Although no breakthrough was occurred when sampling volume was 3L. Finally, in environment of temperature around 22℃, breakthrough may not occurred up to 20ppm during sampling for VCM. During sampling for VCM in environment of temperature around 40℃, no breakthrough occurred in 1-5ppm and 10-20ppm when sampling volume is 5L and 3L respectively. This result suggests that the sampling volume should be considered when VCM sampling under hot conditions (>22℃) by the NIOSH method No. 1007.