Diagnostic Accuracy of Elevated Serum Carcinoembryonic Antigen for Recurrence in Postoperative Stage II Colorectal Cancer Patients: Comparison With Stage III

김호승,이민로 대한대장항문학회 2013 Annals of Coloproctolgy Vol.29 No.4

Purpose: Elevated levels of serum carcinoembryonic antigen (CEA) following a curative resection of colorectal cancer (CRC) indicate recurrence; however, the levels of CEA may be elevated above the normal limit without recurrence. The aim of this study is to analyze the diagnostic accuracy of elevated serum CEA for predicting recurrence in postoperative stage II and stage III CRC patients. Methods: A total of 336 stage II and stage III CRC patients who underwent a curative resection between January 2005 and October 2009 were enrolled. Sensitivities, specificities, positive predictive values (PPVs), negative predictive values (NPVs), likelihood ratios and post-test probabilities of recurrence associated with elevated CEA were analyzed and compared. Results: The median follow-up duration was 45 months (36 to 134 months). Twenty-seven of 189 stage II patients (14.3%) and 52 of 147 stage III patients (35.4%) developed recurrence during the follow-up period. Sensitivities, specificities, PPVs, and NPVs of elevated CEA were 37.0%, 91.4%, 41.7%, and 89.7%, respectively, in stage II patients and 46.2%, 90.5%, 72.7%, and 75.4% in stage III patients. Post-test probabilities of recurrence associated with elevated CEA were 41.8% in stage II patients and 71.9% in stage III patients. Conclusion: The predictive performance of the probability of recurrence associated with elevated serum CEA after a curative resection in stage II CRC patients is lower than that in stage III CRC patients.

유기주석화합물(Tributyltin Acetate)이 미성숙 생쥐의 정소 내 스테로이드 생합성효소 유전자의 발현에 미치는 영향

김호승,김슬기,김태형,한주리,이창주,이재성,윤용달 대한남성과학회 2005 The World Journal of Men's Health Vol.23 No.2

Clostridium difficile Infection After Ileostomy Reversal

김호승,강재현,김한길,Young Hun Kim,배현우,김남규 대한대장항문학회 2021 Annals of Coloproctolgy Vol.37 No.S

Clostridium difficile infection (CDI) after ileostomy reversal is rare, with few reports available in the available literature describing this condition. The diagnosis of CDI after ileostomy reversal is challenging because symptoms such as diarrhea observed in these patients can occur frequently after surgery. However, CDI can be fatal, so early diagnosis and prompt treatment are important. We discuss 2 patients with positive C. difficile toxin assay results on stool cultures performed after ileostomy reversal. Clinical progression differed between these patients: one patient who presented with severe CDI and shock was successfully treated following a prolonged intensive care unit stay for the management of vital signs and underwent hemodialysis, while another patient showed symptoms of mild colitis but we could not confirm whether diarrhea was associated with CDI or with the usual postoperative state. To our knowledge, these represent 2 of just a few cases reported in the literature describing CDI after ileostomy reversal.

Differential Down-regulation of Zeaxanthin Epoxidation in Two Rice (Oryza sativa L.) Cultivars with Different Chilling Sensitivities

김호승,Minh Hien Hoang,전영아,Guangxi Wu,이춘환 한국식물학회 2017 Journal of Plant Biology Vol.60 No.4

When the leaf segments of rice (Oryza sativa L.)plants were subjected to chilling in the moderate light,zeaxanthin (Zx) formation was faster in a chilling-tolerantDongjin-byeo (DJ) than in a chilling-sensitive IR841. Althoughthe rate of Zx formation was accelerated by the treatment of5 mM salicylaldoxime, an inhibitor of Zx epoxidase (ZE),there was almost no changes in DJ. A similar result wasobserved when leaf segments were treated with 50 mMsodium fluoride, a potent inhibitor of chloroplast phosphatase. The slow Zx epoxidation in IR841 during light-chilling wasconfirmed in leaf segments treated with 10 mM dithiothreitol,an inhibitor of violaxanthin de-epoxidase (VDE). However,the differences between the two cultivars were not observedat 25oC. These results suggest that compared with IR841 thehigher rate of Zx formation in DJ is not due to the higherVDE activity in DJ but is due to more rapid down-regulationof ZE in DJ, possibly by its phosphorylation. Compared withDJ, IR841 accumulated more superoxide with PSI inactivationduring light-chilling, which eliminates the possibility of increasedZE down-regulation in DJ leaves by photo-oxidation. Invitro study with alkaline phosphatase supports the idea ofdown-regulation of ZE by phosphorylation under light-chillingcondition. We propose that this reversible down-regulation ofZx epoxidation possibly by the phosphorylation of ZE is animportant regulation mechanism of violaxanthin cycle thatconfers chilling tolerance of a rice cultivar under chillingstress in the light with moderate intensities.

충주댐 수문부 댐체의 지진 안전성 평가

김호승,이지호 동국대학교 산업기술연구원 2008 산업기술논문집 Vol.18 No.1

중력식 콘크리트 댐인 충주댐의 지진 안전성 평가를 위하여 댐의 가장 취약한 부분인 수문부 댐체 절편(monolith)을 대상으로 3차원 선형동해석을 수행하였다. 해석모형에 작용하는 하중으로 정수압, 동수압, 댐의 자중 및 Koyna 수평 및 수직 지반운동을 고려하였으며, 댐의 수위와 지반운동 규모를 고려하여 모두 네 가지 상황에 대해서 평가하였다. 이를 통해 댐 수위가 지진하중에 대한 거동을 변화시키는 중요한 인자라는 사실을 확인하였다. 또한, 해석모형을 통해 최대인장응력을 확인해 본 결과 충주댐이 강진에 매우 취약하다는 것을 알 수 있었다. Three dimensional linear dynamic analysis of a floodgate monolith of Choong-Joo dam is performed to evaluate its earthquake safety under strong earthquake ground motions. Hydro-static, hydro-dynamic and gravity forces are loaded as well as the horizontal and vertical ground motions from the Koyna earthquake data. Four test cases are generated using full/half water levels and ground acceleration inputs. From the present research, it is concluded that the reservoir water level is one of the most important factors affecting the dam behavior subjected to earthquake and Choong-Joo dam is highly vulnerable to strong ground motions.

프로테이믹스를 이용한 내분비계 교란물질 환경독성 연구

김호승,계명찬 한국환경생물학회 2003 환경생물 : 환경생물학회지 Vol.21 No.2

군제대 후 경력단절 외과의사의 진로 탐색기

김호승 대한외과학회 2023 대한외과학회 학술대회 초록집 Vol.2023 No.5

한국 여수 연안에 출현하는 참문어(Octopus vulgaris)의 위내용물 조성

김호승,진수연,문성용,김희용,백근욱 한국수산과학회 2023 한국수산과학회지 Vol.56 No.5

The diet composition of octopus Octopus vulgaris was studied using 392 specimens collected from January to December 2020 in the coastal waters of Yeosu, Korea. The body weight ranged from 86.4 to 3,645.4 g. The most important prey item in the diet of O. vulgaris was decapods, which constituted 43.9% in ranking index (%RI). The second largest prey item was Pisces (%RI=27.9%), and cephalopods (%RI=21.8%) constituted the third largest prey item. The average trophic level of O. vulgaris was 3.97±0.59. Ontogenetic changes were significant among size classes (<400 g, 400–800 g, ≥800 g). The small size class (<400 g) mainly fed on decapods, whereas the medium (400–800 g) and large size (≥800 g) classes mainly fed on cephalopods and Pisces, respectively. The dietary composition varied significantly with season.

Potential Importance of Proteomics in Research of Reproductive Biology

김호승,윤용달 한국발생생물학회 2004 발생과 생식 Vol.8 No.1

프로테오믹스(proteomics, 단백질체학이라고도 함)의 잠재적 중요성은 간질환, 심장질환, 몇몇 종류의 암 등의 의학, 생식 독성, 발생 독성, 생체 독성 연구 분야에서도 명백하게 제시되었다. 그러나 단백질을 대상으로 연구하여 유전자 기능을 연구하는 프로테오믹스 연구를 각각의 분야에 접목시키려는 노력은 아직까지 빈약하다. 프로테오믹스는 기능을 갖는 단백질들의 발현을 종합적이고 정량적으로 측정하는 가장 직접적인 수단이고, 질병, 약물투여, 쇼크, 내분 The potential importance of proteomic approaches has been clearly demonstrated in other fields of human medical research, including liver and heart disease and certain forms of cancer. However, reproductive researches have been applied to proteomics poorly. Proteomics can be defined as the systematic analysis of proteins for their identity, quantity, and function. It could increase the predictability of early drug development and identify non-invasive biomarkers of toxicity or efficacy. Proteome analysis is most commonly accomplished by the combination of two-dimensional gel electrophoresis(2DE) and MALDI-TOF(matrix-assisted laser desorption ionization-time of flight) MS(mass spectrometry) or protein chip array and SELDI-TOF(surface-enhanced laser desorption ionization-time of flight) MS. In addition understanding the possessing knowledge of the developing biomarkers used to assess reproductive biology will also be essential components relevant to the topic of reproduction. The continued integration of proteomic and genomic data will have a fundamental impact on our understanding of the normal functioning of cells and organisms and will give insights into complex cellular processes and disease and provides new opportunities for the development of diagnostics and therapeutics. The challenge to researchers in the field of reproduction is to harness this new technology as well as others that are available to a greater extent than at present as they have considerable potential to greatly improve our understanding of the molecular aspects of reproduction both in health and disease.

프로테오믹스를 이용한 내분비계 교란물질 환경독성 연구

김호승,계명찬 한국환경생물학회 2003 환경생물 : 환경생물학회지 Vol.21 No.2

환경오염이 심각해짐에 따라 국내외적으로 환경에 대한 관심이 고조되고 인체에 해를 끼치는 환경요인으로부터 방어하기 위한 많은 노력들이 기울여지고 있다. 특히 내분비계 장애물질이 생식기능과 면역기능을 약화시키고, 행동 이상을 일으키며, 암 발생률을 높인다는 점이 밝혀지기 시작하면서 많은 연구들이 발표되고 여러 가지 방법들이 내분비계 장애물질과 더불어 환경분야 연구에 응용되어 왔지만 단백질을 대상으로 연구하여 유전자 기능을 연구하는 프로테오믹스(proteomics)연구를 접목시키려는 시도가 아직까지는 빈약하다. 프로테오믹스는 기능을 갖는 단백질들의 발현을 종합적이고 정량적으로 측정하는 가장 직접적인 수단이고, 질병, 약물투여, shock등 생물학적인 동요(perturbation)에 의하여 변하는 단백질들의 발현양상의 변화를 정확하제 관찰할 수 있으며, 유전자발현의 궁긍적인 양상을 규명할 수 있고, 또한 유전자, 단백질 및 질병간의 연결고리를 제공한다. 기존의 biomarker는 다른 질병 표지자와 연관성이 높아 직접적인 유해물질 노출 위험도를 정확히 판정하기 어렵다. 따라서 대량발굴탐색(high-throughput screening)이 가능한 2차원 전기영동 분석과 MALDI-TOF 또는 protein chip array와 SELDI-TOF에 의한 단백질 분자구조 분석기술 및 이들을 지원하는 생물정보학(bioinformatics)의 발전을 이용하여 환경독성 연구에 이용할 수 있는 표적단백질(biomarker)발굴에 적절한 이용이 가능할 것이다. It is important to understand the potential human health implications of exposure to environmental chemicals that may act as hormonally active agents. It is necessary to have an understanding of how pharmaceutical and personal care products and other chemicals affect the ecosystem of our planet as well as human health. Endocrine disruption is defined as the ability of a chemical contaminating the workplace or the environment to interfere with homeostasis, development, reproduction, and/or behav- ior in a living organism or it's offspring. Certain classes of environmentally persistent chemicals such as polychlorinated iphenyls (PCBs), dioxins, fnrans, and some pesticides can adversely effect the endocrine systems of aquatic life and terrestrial wildlife. Research continues to support the theory of ndocrine disruption. However, endocrine disruption researches have heen applied to proteomics poorly. Proteomics can be defined as the systematic analysis of proteins for their identity, quantity and function. It could increase the predictability of early drug development and identify non-invasive hiomarkers of toxicity or efficacy. Proteome analysis is most commonly accomplished hy the combination of two-dimensional gel eleetrophoresis(2D/ E) and MALDI-TOF mass spectrometry (MS) or protein chip array and SELDI-TOF MS. Proteomics have an opportunity to play an important role in resolving the question of what role endoerine disruptors play in initiating human disease. Proteomics can also play an important role in the evaluation of the risk assessment and use of risk management and risk communication tools required to address public health concerns related to actions of endocrine disruptors. Understanding the need for the proteomics and possessing knowledge of the developing biomakers used to assess endocrine activity potential will he essential components relevant to the topic of endocrine disruptors.