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기니픽 망막에서 개시형 콜린성 아마크린세포에 연접하는 칼레티닌 면역반응성 신경절 세포
김형정,서울,문정일,최규룡,Hyung-Chung Kim,M,D,Wool Suh,M,D,Jung-Il Moon,M,D,Kyu-Ryong Choi,M,D 대한안과학회 2008 대한안과학회지 Vol.49 No.2
`Purpose: To demonstrate the characterization calretinin-immunoreactive displaced amacrine cells in the ganglion cell layer using immunohistochemistry and electron microscopy. Methods: For immunohistochemistry, sections from guinea pig retina were incubated with mouse monoclonal antibody directed against calretinin. For double label studies, sections were incuated in mixture of mouse monoclonal anti-calretinin or rabbit polyclonal anti-calretinin with following antibodies : goat polyclonal anti-ChAT, rabbit polyclonal anti-GABA, mouse monoclonal anti-GABAA receptor α1, β2/3. Sections were analyzed using Bio-rad Radiance Plus confocal scanning microscope. Stained sections from three guinea pig were observed with transmission electron microscope. Results: Calretinin immunoreactivity was present in displaced amacrine cells and ganglion cells gaving rise to processes ramified in the inner part of the inner plexiform layer in stratum 4. The same stratum was also occupied by the dendrites of ON-cholinergic amacrine cells. Double-labeling demonstrated that dendrites and cell bodies of displaced amacrine cells colocalized with ON-cholinergic amacrine cells and dendrites of ganglion cells directly overlapped with dendrites of ON-cholinergic amacrine cells. The synaptic connectivity was identified by electron microscopy. Ganglion cell dendrites received synaptic input from ON-cholinergic amacrine cell. GABAA receptor β2/3 subunit bands cofaciculates the dendrites of displaced amacrine cell and ganglion cell that are juxtapose to the α1 subunit of GABAA receptor. Conclusions: These results indicate that ON-cholinergic amacrine cells modulate calretinin-labeled ganglion cell via GABAA receptor β2/3 in the guinea pig retina. `
수정체낭 크기 측정을 위한 수정체낭확장고리의 개발 및 술 전 예측인자에 관한 연구
차영동,김형정,이도형,Young Dong Cha,Hyung Jung Kim,Do Hyung Lee 대한안과학회 2006 대한안과학회지 Vol.47 No.2
Purpose: This study evaluates the capsular bag diameter (CBD) with a capsular tension ring (CTR) following cataract surgery and analyzes the predictive factors correlated with capsular bag size to predict CBD preoperatively. Methods: We inserted the CTR before intraocular lens (IOLs) implantation in 25 eyes undergoing cataract surgery. The axial length (AL), anterior chamber depth (ACD), and lens thickness were measured and keratometry and corneal size were checked preoperatively. Also, the continuous curvilinear capsulorhexis size was measured at 7 days postoperative, and CBD was calculated with CTR in a dilated state at 1day, 1week, 1 month, and 2 months postoperative. Results: The mean CBD gradually decreased from 11.30±1.76 mm at 1 day, 10.86±1.06 mm at 1 week, 10.23±1.43 mm at 1 month, and 9.88±1.31 mm at 2 months. The AL (R=0.657), ACD (R=0.481), and corneal size (R=0.348) had a positive correlation with CBD at 1 week postoperative. The AL (P=0.000) and ACD (p=0.015) were statistically significant predictive factors for CBD. Conclusions: Calculating CBD with the CTR helps to improve visual acuity following cataract surgery after implantation of IOLs having appropriate sizes predicted with AL, ACD, and corneal size.