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고추 에탄올 추출물의 항산화 효과 및 생리활성에 관한 연구
김헌중(Hun-Joong Kim),홍충의(Chung-Oui Hong),남미현(Mi-Hyun Nam),하영민(Young-Min Ha),이광원(Kwang-Won Lee) 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.6
고추(Capsicum annuum)의 항산화 효과 및 생리활성 측정을 통하여 기능성식품의 소재로 활용하기 위한 기초 연구를 진행하였다. 폴리페놀 함량은 과육보다는 씨에서 높게 나타났다. 그러나 플라보노이드 함량은 풋고추 과육에서 높은 함량을 보였다. DPPH radical 소거활성에서 SC50값은 홍고추 씨 1,935±162 ㎍ DM/mL, ABTS radical 소거활성에서는 SC50값이 풋고추 씨 3,354±76 ㎍ DM/mL로 과육에 비해 높았다. 최종당화산물(AGEs) 생성 억제활성은 풋고추와 홍고추 씨, 풋고추와 홍고추 과육 순으로, α-glucosidase 저해활성은 풋고추 과육, 홍고추 씨, 홍고추 과육, 풋고추씨 순이었다. Tyrosinase 억제활성은 홍고추와 풋고추 씨, 홍고추와 풋고추 과육 순이었다. 종합해보면 기능성식품 소재로서 홍고추 씨 및 풋고추 씨가 과육보다 활용도가 높은 것으로 결론지을 수 있다. The goal of this study is to determine the activities of antioxidants and antiglycation from the extracts of various Capsicum annum (known as pepper) ethanolic extract (CAE). We tested the extracts of Capsicum annum seeds and pericarps using 70% ethanol. The CAE showed antioxidant activities in a 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical cation assay, 1,1-diphenyl-2-picrylhydrazyl free radical-scavenging assay, ferric-reducing antioxidant power assay, total flavonoid content, and total polyphenol content. Also, the physiological activities of CAE on glycation inhibition activities, anti-α-glucosidase activities, and tyrosinase activities were measured. As a result, green and red Capsicum annuum seeds show higher levels of antioxidant activities. In addition, the physiological activities are also more effective in the seeds than in the plant pericarps. A radar chart proves that antioxidants and physiological activities are more effective coming from the seeds. And the red Capsicum annuum seeds are more effective than the green ones.
세엽 한국들잔디 변이체 식별을 위한 SCAR 마커 개발
정성진(Sung Jin Chung),박수정(Su Jeong Park),최영인(Young In Choi),김인경(In-Kyung Kim),이가연(Ka-Yeon Lee),김헌중(Hun- Joong Kim),이긍주(Geung-Joo Lee) 충남대학교 농업과학연구소 2013 농업과학연구 Vol.40 No.2
Polymorphic bands of two fine-leaf zoysiagrass mutants (CNU 70-1, CNU 70-2) induced via a gamma-ray irradiation on seeds of Zoysia japonica were obtained by using randomly amplified polymorphic DNA (RAPD) primers. The genotype-specific fragments were then converted into PCR-based sequence characterized amplified region (SCAR) markers, which are now amenable to detecting them among other zoysiagrass species widely noticeable in Korea. The CNU 70-1-specific primer set amplified about 900 bp successfully, while the CNU 70-6 marker produced the expected 1,500 bp band, by which those markers were nominated by CNU 70-1_900 and CNU 70-6_1500 SCARs, respectively. The developed SCAR markers can be an applicable tool in sod industry where illegal appropriation hampers breeder’s right and profits due to the turfgrass plant vegetatively propagating.