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정상인과 만성 부비동염 환자에서 PCR과 진균배양을 이용한 진균검출의 비교
김향복,김선태,양시호,박정수,정유삼,차흥억,서일혜,황유진,이희승 대한이비인후과학회 2002 대한이비인후과학회지 두경부외과학 Vol.45 No.7
Background and Objectives:Fungi are found in normal flora in various amounts. However, fungi in chronic sinusitis are poorly understood, and it is not clearly defined whether it is a pathogen or simply a part of normal flora. The polymerase chain reaction (PCR)aim of this study was to compare the sensitivity of conventional culture technique and PCR analysis in detecting fungus of the nasal cavity and to compare fungal species found in normal controls and patients with chronic sinusitis. Materials and Method:Sterile saline irrigation was performed and then mucin filled saline was collected from the nasal cavity of patients with chronic sinusitis and normal controls. Fungus culture and PCR analysis were carried out with irrigated saline. Results:PCR for panfungal gene was positive in 92.5% and 97.5% while fungus cultures were positive in 23.3% and 30.5% of pat-and species of fungus betwen patients with chronic sinusitis and normal controls. Conclusion:PCR for panfungal gene was more sensitive for fungus detection than fungus culture in patients with chronic sinusitis and normal controls. Presence of fungus alone was insuficient to implicate it as the pathogen of chronic sinusitis. It will be necessary to study further about fungal species in patients with chronic sinusitis and normal controls. (Korean J Otolaryngol 2002;45:666-70)