Effects of Radioprotective Ginseng Protein on MMS Induced Sister Chromatid Exchanges and Chromosome Aberrations

김춘미,박혜숙,Kim, Choon-Mi,Park, Hae-Sook 생화학분자생물학회 1988 한국생화학회지 Vol.21 No.4

CHO-K1 세포에 MMS를 가하여 높은 반도의 자매염색분체교환 (SCE)과 염색체이상 (CA)을 유도 한 후, 한국인삼으로부터 분리, 정제한 방사선 방어효과가 있는 인삼단백질을 가하여 그 영향을 연구 하였다. SCE에 대한 연구에서, MMS의 용량을 $10^{-5}M$에서 $10^{-3}M$까지 증가시킴에 따라 그 빈도는 몇 배로 증가하였으며, 인삼단백질 500 ${\mu}g/ml$을 MMS 처리전과 후에 배양세포에 가했을 때, 그 빈도는 유의성 있게 감소하였다. 또한 $10^{-3}M$ MMS에서는 전처리의 경우에 그 효과가 더 현저하였다. 인삼단백질의 양을 100에서 500 ${\mu}g/ml$까지 증가시켰을 때, $10^{-5}M$, $10^{-4}M$, $10^{-3}M$ MMS의 각 농도에서, 그 빈도는 처음 100 ${\mu}g/ml$ 첨가시에는 크게 감소하였으나, 그 이상의 농도에서는 거의 plateau를 나타내었다. $10^{-3}M$ MMS에서는 전처리의 경우 후처리보다 SCE 빈도의 감소가 더 현저하였다. CA에 대한 연구에서, MMS의 용량을 $10^{-3}M$에서$5{\times}10^{-3}M$까지 증가시킴에 따라 그 빈도는 수배로 증가하였으며, 인삼단백질 500 ${\mu}g/ml$을 MMS 처리 후에 가했을 때 그 빈도는 유의성있게 감소하였다. 인삼단백질의 양을 200에서 500 ${\mu}g/ml$까지 증가시켰을 때, $10^{-3}M$, $2.5{\times}10^{-3}M$, $5{\times}10^{-3}M$ MMS의 각 농도에서, 그 빈도는 계속적으로 감소하였다. 이 결과는 방사선 방어작용이 있는 인삼단백질이 SCE와 CA로 연결되는 DNA 상의 손상을 감소시킴을 의미하며, 이러한 효과는 이 단백질이 DNA repair 과정의 어느 단계에 작용하거나, DNA 분자 자체를 안정화시킴으로써 나타날 수 있다고 생각된다. Radioprotective ginseng protein was purified from Korean ginseng and its effects were studied on the frequencies of sister chromatid exchanges (SCE) and chromosome aberrations (CA) induced by MMS in CHO-K1 cells. In SCE study, as the dose of MMS was increased from $10^{-5}M$ to $10^{-3}M$, the frequency was markedly increased to several fold. When the radioprotective ginseng protein was added to the cells at $500\;{\mu}g/ml$, before and after MMS treatment, the frequency was decreased significantly at all MMS doses and more in the case of pre-treatment especially at the concentration of $10^{-3}M$ MMS. When the concentration of the ginseng protein was increased from 100 to 500 ${\mu}g/ml$, at each dose of $10^{-5}M$, $10^{-4}M$ and $10^{-3}M$ MMS, the reduction of SCE frequency was sharp at the first addition of 100${\mu}g/ml$ and then reached a sort of plateau at all higher doses. At $10^{-3}M$ MMS, the pre-treatment resulted in significantly lower SCE than the post-treatment. In CA study, as the dose of MMS was increased from $10^{-3}M$ to $5{\times}10^{-3}M$ the frequency was increased sharply to many fold. When the radioprotective ginseng protein was added after MMS treatment, the frequency was reduced significantly at all MMS doses. When the concentration of the ginseng protein was increased from 200 to 500 ${\mu}g/ml$, at each dose of $10^{-3}M$, $2.5{\times}10^{-3}M$ and $5{\times}10^{-3}M$ MMS, the reduction of CA proceeded rather continuously at all three doses. The results seem to suggest that the radioprotective ginseng protein reduces DNA damage that leads to the formation of SCE and CA, possibly by acting at certain steps of repair processes and/or by stabilizing the DNA molecule.

방사선 방어효과가 있는 인삼단백성분이 MMS에 의해 유도된 자매염색분체 교환과 염색체이상에 미치는 영향

김춘미,박헤숙 ( Choon Mi Kim,Hae Sook Park ) 생화학분자생물학회 1988 BMB Reports Vol.21 No.4

Radioprotective ginseng protein was purified from Korean ginseng and its effects were studied on the frequencies of sister chromatid exchanges (SCE) and chromosome aberrations (CA) induced by MMS in CHO-K1 cells. In SCE study, as the dose of MMS was increased from 10^(-5)M to 10^(-3)M, the frequency was markedly increased to several fold. When the radioprotective ginseng protein was added to the cells at 500㎍/㎖, before and after MMS treatment, the frequency was decreased significantly at all MMS doses and more in the case of pre-treatment especially at the concentration of 10^(-3)M MMS. When the concentration of the ginseng protein was increased from 100 to 500㎍/㎖, at each dose of 10^(-5)M, 10^(-4)M and 10^(-3)M MMS, the reduction of SCE frequency was sharp at the first addition of 100㎍/㎖ and then reached a sort of plateau at all higher doses. At 10^(-3)M MMS, the pre-treatment resulted in significantly lower SCE than the post-treatment. In CA study, as the dose of MMS was increased from 10^(-3)M to 5×10^(-3)M, the frequency was increased sharply to many fold. When the radioprotective ginseng protein was added after MMS treatment, the frequency was reduced significantly at all MMS doses. When the concentration of the ginseng protein was increased from 200 to 500㎍/㎖, at each dose of 10^(-3)M, 2.5×10^(-3)M and 5×10^(-3)M MMS, the reduction of CA proceeded rather continuously at all three doses. The results seem to suggest that the radioprotective ginseng protein reduces DNA damage that leads to the formation of SCE and CA, possibly by acting at certain steps of repair processes and/or by stabilizing the DNA molecule.

한국 작곡가 연구를 둘러싼 가치 판단의 쟁점들

김춘미 ( Choon Mi Kim ) 한국음악학학회 2005 음악학 Vol.12 No.-

인삼 단백질분획에 대한 폴리아크릴아마이드 전기영동

김춘미(Choon Mi Kim),황정주(Jung Joo Hwang) 大韓藥學會 1986 약학회지 Vol.30 No.6

Korean ginseng was purified to obtain radioprotective protein fractions by buffer extraction, ammonium sulfate fractionation, CM-cellulose column chromatography, heat inactivation and Sephadex G-75 column chromatography. The final three fractions, GI, GII and GIII were subjected to Disc-polyacrylamide gel electrophoresis (PAGE) and SDS-PAGE. The molecular weights (M.W.) of native and denatured proteins were estimated by using regression line equations obtained from the mobilities of standard proteins. As the results, in Disc-PAGE, the GI fraction showed two protein bands with M.W. of above 213,000 and 55,000, GII showed one band with M.W. of 44,000 and GIII, also one band with M.W. of 19,000. In SDS-PAGE, GI fraction gave four subunit bands with M.W. of above 114,000, 27,000, 24,000 and 19,000, GII gave two bands with M.W. of 46,000 and 22,000 and GIII, one band of 19,000.

방사선 방어작용이 있는 인삼 단백분획의 CHO-KI 세포에 대한 세포 독성

김춘미(Choon Mi Kim),윤석란(Suk Ran Yoon) 대한약학회 1988 약학회지 Vol.32 No.5

Radioprotective ginseng protein fraction was isolated from Korean white ginseng and its cytotoxic effect on CHO-KI cells was studied by the method of measuring the relative cell survival and total cellular protein content (FRAME method). When ginseng protein at the dose of 300, 600, 900, 1200mcg/ml was treated to cells for 24 hrs, the relative survival was significantly decreased at the concentration of above 600mcg/ml, indicating the presence of cytotoxic effect of the protein at certain concentration. When cellular protein content was measured after ginseng protein at the dose of 300, 600, 900, 1200mcg/ml was treated, the amount of cellular protein was significantly reduced at the concentration above 600mcg/ml in the case of 24 hr treatment and at all concentrations including 300mcg/ml in the case of 72 hr treatment. The data suggest that the protein may inhibit cell growth, resulting in the reduction of live cells in culture. ID50 value which is the concentration of ginseng protein that reduces the total cellular protein content to 50% of the control was calculated as 2276.86 and 1323.32mcg/ml in groups treated for 24 and 72 hr, respectively. Since ID50 value of above 1000mcg/ml indicates very weak cytotoxicity, the ginseng protein seems to exert very weak cytotoxic effect on CHO-KI cells.

세계화 담론과 한국음악의 선택

김춘미(Kim Choon-Mi) 한국학중앙연구원 2002 한국학 Vol.25 No.3

인삼단백질의 방사선 방어작용

김춘미(Choon Mi Kim),한규선(Gyu Sun Han) 대한약학회 1985 약학회지 Vol.29 No.5

Ginseng proteins were isolated and partially purified to obtain two fractions, namely GI and GII. Radioprotective effects of these fractions were examined on gamma-ray irradiated ICR mice by observing 30-day survival rates after irradiation. Also investigated were the effects of GI fraction on the recovery of radiation damage. As the results, the GI fraction showed strong protection against radiation indicated by the increment of 30-day survival rates, while the GII fraction did not. The GI fraction enhanced the recovery of body and splenic weights and increased the amount of DNA in liver significantly. It also helped to recover the damage done on erythrocytes by increasing the number to normal in short period, however, it had no effect on the recovery of leukocyte counts.

Beta-lactamase 저해제 이성체의 결합양상과 활성관계

목성희,최인희,박인선,김춘미,Mok, Sung-Hee,Choi, In-Hee,Park, In-Sun,Kim, Choon-Mi 대한화학회 2005 대한화학회지 Vol.49 No.5

Correlation between the Binding Mode and Inhibitory Activity of Benz[f]indole-4,9-dione Analogs with Human Topoisomerase I-DNA

최인희,우선아,박혜숙,박혜영,김춘미,Choi, In-Hee,Woo, Sun-A,Park, Hae-Sook,Park, Hea-Young,Kim, Choon-Mi Korean Chemical Society 2005 대한화학회지 Vol.49 No.5

인삼 사포닌이 일산화탄소중독 및 노화과정에서 흰쥐의 신경전달물질 함량 변화에 미치는 영향

박혜영(Hea Young Park),김춘미(Choon Mi Kim),주지연(Ji Yeon Ju),최현진(Hyun Jin Choi) 대한약학회 1992 약학회지 Vol.36 No.3

After rats were exposed to 5,000 ppm carbon monoxide for 30 minutes, the amounts of catecholamine neurotransmitters in stratum were measured using high performance liquid chromatograph equipped with electrochemical detector. The concentration of dopamine in stratum was significantly decreased after carbon monoxide intoxification, but those of dihydroxyphenylacetic acid, norepinephrine, and epinephrine was not changed. However the pretreatments of Ginseng total saponin and panaxatriol saponin increased the concentrations of dopamine and its acidic metabolites (DOPAC and HVA). Ginseng total saponin also increased the concentrations of norepinephrine and epinephrine. Similar results were obtained from aged rats.