행동이상 Pogo마우스 솔기핵 Serotonin 면역반응 신경세포의 증가

김철태,한승연,정재현,윤대성,이서울,Richard Hawkes,이남섭,정영길 대한해부학회 2005 Anatomy & Cell Biology Vol.38 No.5

본 연구는 한국산 야생마우스(KJR/Mskist)에서 유래된 선천성 행동이상마우스인 pogo마우스 중간뇌솔기핵 serotonin 면역반응 신경세포의 발현양상을 면역조직화학적 방법으로 확인하여 pogo마우스에서 관찰되는 과도한 폄운동을 동반한 비정상적인 행동의 발생과의 상관관계를 밝히고자 계획되었다. 본 연구를 통하여 다음과 같은 결과를 얻었다. 첫째, 행동이상 pogo마우스 중간뇌 솔기핵의 serotonin 면역반응 신경세포가 대조군에 비하여 유의하게 증가되었음을 확인하였으며, 둘째 행동이상 pogo마우스 솔기핵의 serotonin 면역반응 신경세포의 증가는 솔기핵 전부위에서 관찰되지 않고 부위에 따라 상이하였다. 즉, 등쪽솔기핵의 등쪽세포군, 배가쪽세포군 및 정중솔기핵에서는 serotonin 면역반응 신경세포가 증가되었지만 등쪽솔기핵의 배쪽세포군과 다발사이세포군에서는 대조군과 차이가 없었다. 이상의 결과는 pogo 마우스에서 관찰되는 비정상적 뒷다리 폄운동을 포함한 행동이상이 중간뇌 솔기핵의 serotonin 발현 증가와 상관관계가 있을 것으로 생각되며 궁극적으로 소뇌연구, 각종 신경활성물질의 기능연구를 위한 모델동물로 사용되기 위한 pogo 마우스의 뇌에 대한 중요한 기초자료로 사용될 수 있을 것으로 생각된다. This study was carried out to investigate the distribution of serotonin-immunoreactive neruons in the raphe nucleus of the ataxic pogo (pogo/pogo) mice derived from a Korean wild mice. Using by immunohistochemistry, we undertook to elucidate any correlation between the serotonin expression and behavior ataxia including abnormal hindlimb extension in the ataxic pogo mice. The present study has two important findings. First, serotonin immunoreactivity was increased in the raphe nucleus of the ataxic pogo mice. Second, serotonin immunoreactivity was different with the region of raphe nucleus. In the dorsal part of dorsal raphe nucleus (DRD), ventrolateral part of dorsal raphe nucleus (DRVL) and median raphe nucleus (MR), serotonin immunoreactivity was increased, whereas the ventral part of dorsal raphe nucleus (DRV) and interfascicular part of dorsal raphe nucleus (DRI) was similar with the control mice. Therefore, elevated expression of the serotonin in the raphe nucleus of ataxic pogo mice might be a source of behavior ataxia and may be related with the induction of the ataxic phenotype including abnormal hindlimb movements.

위 지방증식증 1예

김철태,최준,권지혜,김지민,임수진,김지섭,박재석,석지혜,김용욱,최정,박승근,박희욱,옥종한,김혜숙 대한소화기내시경학회 2002 Clinical Endoscopy Vol.25 No.1

Lipohyperplasia is a lesion characterized by diffuse infiltration of lipocyte in the submucosal layer, which was formally known as 'lipomatosis' or 'lipomatous hyperplasia'. Lipohyperplasia is distinguished from lipoma, the former is not encapsulated and has normal mucosa. Most of lipohyperplasia developed at the ileocecal valve, showed clinical manifestation of abdominal pain, rectal bleeding, intestinal intussuception and intestinal obstruction. We experienced a case of a 45-year-old woman who had polypoid lipohyperplasia of the stomach. Her chief complaint was recurrent epigastric discomfort. Endoscopic finding was a 10 9 mm sized polypoid lesion in the anterior wall of lower body of the stomach. Endoscopic polypectomy was performed and the histological examination revealed infiltration of lipocyte in the submucosal layer. (Korean J Gastrointest Endosc 2002;25:34-37) 지방증식증은 점막하층에 지방세포의 미만성 침착을 보이는 질환으로 대부분 대장, 직장, 회맹판에서 발생하여 임상증상을 나타내는 질환으로 과거 ‘지방종증’ 또는 ‘지방종성 비후’로 불렸다. 본 증례에서는 건강 검진에서 발견된 위의 용종성 병변에 대하여 용종절제술 시행 후 지방증식증으로 밝혀진 예로, 점막하 종양의 감별진단에 포함되어야 할 것으로 생각한다.

응급알레르기 상황에서 사용하기 쉬운 하이드로겔 제제의 제조 및 평가 과정

김철태,김훈 한국디지털정책학회 2021 디지털융복합연구 Vol.19 No.3

인구비례 응급출동 현황 분석

김철태 한국디지털정책학회 2014 디지털융복합연구 Vol.12 No.12

액티브 러닝 학습방법을 활용한 심전도 개론 및 실습 교과과정의 학습효과와 만족도 조사

김철태,김정선,Kim, Chul-Tae,Kim, Jung Sun 한국응급구조학회 2019 한국응급구조학회지 Vol.23 No.2

Purpose: This study aimed to introduce active learning methods, including flipped, case-based, and team-based learning in an electrocardiography (ECG) course and to investigate outcomes and satisfaction with these methods. Methods: To identify the learning effect of active learning, pre-and post-academic self-efficacy was compared between the experimental and control groups. In the experimental group, pre-and post-knowledge and clinical performance regarding ECG were also assessed. In addition, class satisfaction was investigated after application of active learning methods in the experimental group. Data were collected from 84 paramedic students and analyzed using SPSS 22.0 (IBM, Armonk, NY, USA). Results: The experimental group showed significant improvement in post-academic self-efficacy and knowledge. The experimental group also showed high clinical performance (9.83 out of 10 in ECG checking ability and 9.63 out of 10 in ECG reading ability). The mean satisfaction score was 4.23 out of 5 (responses based on a Likert scale) in the experimental group. Conclusion: Active learning in an ECG course was found to be highly effective and satisfactory. Furthermore, paramedic students can enhance their accountability and judgement with team-based learning through free engagement in discussion.

행동이상 Pogo 마우스 소뇌 Calretinin 면역반응성 홑극붓털세포(Unipolar brush cells)의 분포

김철태(Chul-Tae Kim),박오성(O-Sung Park),한승연(Seung-Yun Han),정재현(Jae-Hyun Jeong),윤대성(Dae-Sung Yoon),이서울(Seoul Lee),Richard Hawkes, 김무강(Moo-Kang Kim),이남섭(Nam-Seob Lee),정영길(Young-Gil Jeong) 대한해부학회 2005 Anatomy & Cell Biology Vol.38 No.4

홑극붓털세포 (Unipolar Brush Cell)은 소뇌 과립층에서 최근 새롭게 발견된 흥분성 사이신경원(excitatory interneuron)으로 포유류 안뜰소뇌(vestibulocerebellum)에 집중적으로 분포한다. 홑극붓털세포는 소뇌외부에서 기원한 이끼섬유(mossy fiber)로부터 내인성 신경연접을 중계하는 역할 뿐만 아니라, GABA성 사이신경세포와 조롱박세포 (Purkinje cell)로 투사하는 흥분성, 글루타민성 사이신경세포로 알려져 있으며, 홑극붓털세포의 가지돌기들은 과립층 내에서 분지하여 이끼섬유, 과립세포 또는 다른 홑극붓털세포들과 시냅스를 형성한다. 본 연구는 새로운 행동이상(ataxia) 모델인 pogo 마우스를 이용하여, 대조군과의 calretinin 양성반응 홑극붓털세포의 분포 양상의 차이를 규명하고, Ca2± 항상성과의 연관관계를 알아보기 위해 시행하였다. 전체적으로 행동이상 pogo 마우스 소뇌에서 calretinin 양성반응 홑극붓털세포는 대조군, 즉 pogo/± 마우스에서의 경우와 동일한 양상으로 소뇌벌레(cerebellar vermis)의 후엽 중 IX, X번 소엽에서 주로 관찰되었다. 그러나 대조군에 비하여 calretinin 면역반응 홑극붓털세포의 전체적인 수의 감소가 관찰되었으며, 대조군에서 홑극붓털세포의 분포가 IX, X 소엽에서 시상면성 배열양상을 보이는 데에 반하여, 행동이상 pogo 마우스 소뇌에서는 시상면성 배열양상을 보이지 않았다. 이와 같은 행동이상 pogo 마우스 소뇌의 calretinin 양성반응 홑극붓털세포수와 배열양상의 비정상성은 소뇌 Ca2± 항상성에 영향을 미칠 것으로 생각되며, 행동이상 pogo 마우스에서 관찰되는 다양한 행동이상과도 밀접한 연관 관계가 있을 것으로 사료된다. Unipolar brush cells (UBCs) are a class of putative interneurons found in the granular layer of mammalian cerebellum and dorsal cochlear nucleus. The unipolar brush cells (UBCs), as with granular cells, which receives afferent synaptic input from extrinsic mossy fiber and whose axons branch in the granular layer and establish a system of cortex-intrinsic mossy fibers, which synapse with granule cells and other UBCs. In general, UBCs have been identified most readily by their expression of the calcium-binding protein, calretinin. The purpose of this study was to provide information about UBCs distributions of the new ataxic animal model, pogo mouse cerebellum using anti-calretinin immunohistochemistry, immunofluorescence and its effect on calcium homeostasis. Through the examination of calretinin immunohistochemistry and immunofluorescence, we observed that many calretinin immunoreactive UBCs were distributed widely throughout the lobules IX and X of the granular layer of both group. But, we found the number of calretinin immunoreactive UBCs of ataxic pogo (pogo/pogo) mouse was decreased and distribution pattern was altered, compared to control mouse. This result also suggest that reduced calretinin expression may effect on cerebellar Ca2± homeostasis, and it may in turn, explain the impaired motor coordination found in the ataxic pogo mice.

119구급대원의 감정노동과 직무스트레스가 피로에 미치는 영향

임완영,김철태 사단법인 한국응급구조학회 2022 한국응급구조학회지 Vol.26 No.1

Purpose: This is a descriptive research study that aims to identify factors affecting fatigue by examining the degree of emotional labor, job stress, and fatigue of 119 emergency medical technicians (119 EMTs). The correlation between emotional labor, job stress, and fatigue was also confirmed. Methods: A total of 139 EMTs working in the Daejeon area participated in this study. Data were collected from July 6-15, 2021. Results: When the number of dispatches was high and the job was dissatisfying, the degree of emotional labor was high. In addition, the shorter the work experience and the lower the job satisfaction are, the higher the job stress is. The degree of fatigue was significantly higher in female than male 119 EMTs, and when they had short work experience and were dissatisfied with their job, the degree of fatigue was higher. Moreover, there was a significant correlation between emotional labor, job stress, and fatigue of the 119 EMTs. Conclusion: To reduce emotional labor, job stress, and fatigue of 119 EMTs, appropriate interventions for each factor and institutional improvements are required.

응급구조사들이 사용하는 약물의 제형 개발에 관한 연구

김훈,김철태 사단법인 한국응급구조학회 2020 한국응급구조학회지 Vol.24 No.2

Purpose: In this study we aimed to manufacture and evaluate an oral disintegrating film containing ibuprofen. Methods: Optimal oral ventilation was manufactured using ibuprofen 3g, polyvinyl alcohol #500 4.2g, HPMC K 100M 1.6g, glycerol 4g, TWEEN #20 0.3g, PEG #20 0.3g, citric acid 0.5g, sucralose 0.1g, ethamol 10mL, and distilled water 30mL. Results: Film mass ranged from 110 to 130mg in all prescriptions, showing general uniformity while the water content ranged from 6 to 12%. Measurement of ibuprofen content in all manufactured film solutions averaged 100.12% (98.0-102.0%). The elution test predicted the time taken from the body and the film agent of all prescriptions was released 100% within 5 minutes to confirm the rapid elution. Conclusion: Based on the results of all test, prescription E was proved to be the most suitable.

신체억제대에 대한 일반인의 인식, 태도, 지식 및 교육경험과 교육요구도 조사

한다연,김철태,Han, Da-Yeon,Kim, Chul-Tae 한국응급구조학회 2018 한국응급구조학회지 Vol.22 No.2

Purpose: This study aimed to investigate recognition, attitudes, knowledge, education experience, and education demand regarding physical restraint among laypersons. Methods: A self-reported questionnaire was completed by 105 randomly recruited laypersons in D city. Excluding 2 incomplete answers, 103 data were analyzed by frequency analysis, t-test, ANOVA, and chi-square test using SPSS 23.0. Results: Laypersons' recognition of physical restraints was rated at 3.37 out of 5 points, and showed a positive perspective. Knowledge on physical restraints was rated at 13.68 out of 18 points and was affected by age and education level (F = 2.845, p = .028)(F = 3.126, p = .029) A majority of the participants had no education on physical restraints. Nevertheless, participants showed intention to receive education in physical restraint to reach further understanding. Conclusion: Education on physical restraints is necessary for lay persons. However, there were education limits for people living in modern times. As an intervention addressing this problem, observing videos on the internet is recommended. Moreover, for advertising/educational purposes, accessing the internet and using smart-phone applications are suggested.

기도확보가 어려운 상황에서 Miller blade와 Macintosh blade를 이용한 기관내삽관의 비교 : 숙련되지 않은 인턴을 대상으로 한 마네킨 연구

이미림,김철태,이효철 사단법인 한국응급구조학회 2018 한국응급구조학회지 Vol.22 No.3

Purpose: The purpose of this study was to compare laryngoscopic views and ease of use and success of intubation, via the percentage of glottic opening (POGO) scale when using the Miller blade and Macintosh blade in paraglossal approach. Methods: Forty intern doctors were randomized for laryngoscopy to be performed in a crossover manner. They performed endotracheal intubation with Miller blade and Macintosh blade in two airway scenarios: normal airway and difficult airway with edema. We observed the rate of successful intubation, time required for visualizing the glottis, time to complete endotracheal intubation, ease of intubation, and the POGO scale. Results: In the normal airway, there was no difference in intubation between the two endoscopes. In the difficult airway, the time for visualizing the glottis (7.80 versus 10.24 sec; p=.006), the time to tube passage (19.38 versus 23.03 sec; p=.038) and the time to complete endotracheal intubation (21.84 versus 28.54 sec; p=.022) with Miller blade was shorter than with Macintosh blade. The POGO scale(%) of the Miller blade was higher than that of the Macintosh blade’s (62.25 versus 56.32; p=.030). Conclusion: Compared to the Macintosh blade, Miller blade provided better visualization of the glottis and POGO scale, and faster time to completion of endotracheal intubation.