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한국재래산양에서 계절이 정소기능, 정자의 내동성 및 수정능력에 미치는 영향 I. 정액성상과 정자의 내동성 및 난자침입능력의 계절적 변화
김창근,정영채,김광식,윤종택,이장희,정영호,최선호,김흥률,김수,권처진,Kim, C.K.,Chung, Y.C.,Kim, K.S.,Yoon, J.T.,Lee, J.H.,Chung, Y.H.,Choi, S.H.,Kim, H.Y.,Kim, S.,Kwon, C.J. 한국동물번식학회 1994 Reproductive & developmental biology Vol.17 No.4
This study was conducted to observe seasonal and individual changes in semen characteristics and sperm freezability, and sperm penetration into zona-free hamster eggs in Korean native goats. Buck response and change in semen characteristics to electrical stimulations was evaluated for four seasons throughout 2 years and percentage of motile sperm and normal apical ridge acrosome was investigated after equilibration and thawing for 4 seasons with 5 bucks. Sperm penetration rate was evaluated for 4 bucks. 1. Probe insertion at depth of 7cm and repeated stimulation for 3 sec was more effective(P<0.05) in buck response and semen collection than those of other conditions. 2. Semen characteristics from electrojaculation was signficantly(P<0.005) higher in spring and fall for semen volume, in spring and summer for sperm concentration and in fall for sperm motility than those in other seasons, respectively. However, there were no differences in total sperm among seasons. 3. Buck response to electrical stimulation showed significant difference(P<0.05) among individuals in all 3 seasons except winter. Significant individual difference in semen volume was only in spring and summer, but there was no indivudual difference in sperm concentration and total sperm in all season. 4. Washing of semen before freezing treatment was greatly(P<0.05) beneficial to sperm motility after thawing, no matter whether ejaculates exhibit egg yolk coagulation or not. 5. Sperm motility after glycerol equilibration was significantly(P<0.05) low in summer semen and motility after thawing was greatly(P<0.05) higher in winter semen than in other seasons. Freezability of unwashed sperm was significantly difference among bucks, but a yearly freezability of washed sperm after chilling and thawing were no differences among bucks and percentage of normal apical ridge acrosome were not different among seasons and bucks. 6. There was no significant difference in sperm motility after thawing between egg yolk levels in summer, although 20% level gave more higher motility than 5% level. 7. In summer, 3.2% glycerol and 3-h equilibration gave greatest percentage(P<0.05) of sperm motility and normal apical ridge acrosome after thawing. 8. Sperm penetration rate into zona-free hamster eggs was not different between bucks and seasons. Overall, it is concluded that to obtain maximum sperm output and successive semen freezing by electrojaculation method, buck selection with good response in all season could be basically considered and that seasonal effect on sperm freezability was more greater than that of individual bucks.
한국재래산양에서의 계절이 정소기능, 정자의 내동성 및 수정능력에 미치는 영향 II. 혈청내 Testosterone수준의 계절 및 하루중 변화
김창근,정영채,김광식,김수,권처진,한기영,윤종택,정영호,이장희,Kim, C.K.,Chung, Y.C.,Kim, K.S.,Kim, S.,Kwon, C.J.,Han, K.Y.,Yoon, J.T.,Chung, Y.H.,Lee, J.H. 한국동물번식학회 1994 Reproductive & developmental biology Vol.17 No.4
Seasonal and diurnal variation in serum testosterone was measured by radioimmunossary in 3 mature Korean native male goats. Blood was collected at 3-h intervals for 24h in each season. Mean levels of 4 seasons ranged from 0.82 to 2.06ng/ml and no difference was among seasons, although level in fall and winter was slightly higher. Diurnal level tended to increase at daytime in all season and when diurnal variation in level was compared to daily mean of each season, level of daytime greatly(P<0.05) increased in summer. However, variation between light and dark period in other seasons did not show significant difference. Daytime increase of testosterone was found 3 to 4 after dawn and level increased 1 to 2h ahead in summer and fall than that in others. These results in serum testosterone suggest that there was no great seasonal variation in endocrine function of goat testis.
미경산돈의 발정유기 및 혈중 Progesterone 수준변화에 관한 연구
김창근,정영채,정영호,김형태 ( C . K . Kim,Y . C . Chung,Y . H . Chung,H . T . Kim ) 한국축산학회 1982 한국축산학회지 Vol.24 No.3
These experiments were conducted to investigate the effects of PGF₂α injection on induction of estrus and change in blood progesterone concentration in normal and anestrous gilts. Six anestrous gilts and 5 normal gilts were used. For induction of estrus either 10㎎ PGF₂α or 10㎎ PGF₂α plus 1,000IU PMSG was intramuscularly injected. Serum progesterone concentration were determined by radioimmunoassay. Conception of gilts were confirmed by necropsy examination for ovary and uterus condition within 35 days after mating. The number of gilts which showed standing or dull estrus after either PGF₂α or PGF₂α plus PMSG injection was 5(83.3%) out of 6 long-anestrous gills and 4(80.0%) out of 5 normal gills. The average interval from PGF₂α injection to the induced estrus was 7.2 days (4 to 12 days) in 5 long-anestrous gills and 13.5 days (6 to 25 days) in 4 normal gills. The number of pregnant gills was 2 out of 4 long-anestrous gills and 3 out of 4 normal gills, except each one get in two groups which was necropsied too early to be confirmed whether pregnant or not, although their ovary and uterus were normal condition. Blood progesterone concentration of 11.0 ng/㎖ before PGF₂α injection in 5 longanestrous gills which showed estrus decreased to 3.0 ng/㎖ at 1 day and 1.3 ng/㎖ (11.8% of initial concentration) at 4 days and increased again to 4.3 ng/㎖ at 8 days after PGF₂α injection, respectively and the concentration of 16.5 ng/㎖ before PGF₂α injection in 4 normal gills which showed estrus decreased to 3.2 ng/㎖ at 1 day and 2.3 ng/㎖ (13.9% of initial concentration) at 4 days and increased to 4.7 ng/㎖ after PGF₂α injection, respectively. Blood progesterone concentrations at 10 to 15 days and 30 to 35 days after mating in pregnant gills were 8.2 to 26.4 ng/㎖ and 17.7 to 19.6 ng/㎖ in 2 anestrous gills and 23.1 to 28.5 and 19.6 to 19.9 ng/㎖ in 3 normal gills respectively. The number of corpus luteum in the induced estrous gifts averaged 18.8 in 3 longanestrous gifts and 15.0 in 4 normal gifts. There would appear to be larger number of CL in PGF₂α plus PMSG injection than in only PGF₂α injection.
과학영재교육기관(科學英才敎育機關)을 위(爲)한 도서관기준설정(圖書館基準設定)에 관(關)한 연구(硏究)(I)
김창근,Kim, Chang-Keun 한국정보관리학회 1987 정보관리학회지 Vol.4 No.1
국가발전의 원동력으로서의 과학기술밭전을 위한 국가적 조치로서 영재교육기관을 설립하고, 교육을 통한 과학영재의 발굴, 육성을 시도하고 있는 싯점에서 교육기관의 환경적 요인이 크게 중요시되고 있다. 그 환경요인 중에서도 지식의 보고로서의 도서관 환경조성이야 말로 소기의 목표를 달성하고 그 시기를 더욱 앞당길 수 있는 가장 중요한 요인이라 할 수 있기 때문에, 본 논문에서는 과학영재교육의 중요성을 기술하고 그 교육을 위한 도서관의 환경적 개선요인을 조사, 비교 분석하여 과학영재교육 기관을 위한 도서관 기준을 시설, 인원, 장서, 예산등의 포괄적인 기준에 관한 합리적 타당성을 도출하고 최적 기준을 제시하였다. Many countries make it their national policy to develop science and technology as the primary element in their overall national development, they concentrate on identifying gifted students in those areas and on educating them in special institutions. At this point the environmental factors in those educational institutions are highlighted. Of all these factors, a favorable and amenable environment of a library, the treasure - house of knowledge, is the most essential to the early achievement of the stated educational aims. Thus, this thesis describes the importance of educating gifted students, and then examines, compares and analyzes the factors involved in improving the environments of institutional libraries for gifted students ; finally, this thesis proposes the minimum and optimum standards for library facilities, personnel, collections, and budgets.
안료의 배합비가 더블 도공지의 물성 및 인쇄적성에 미치는 영향
김창근,이용규,Kim, Chang-Keun,Lee, Yong-Kyu 강원대학교 산림과학연구소 2001 Journal of Forest Science Vol.18 No.-
The main objective of this study was to investigate effects of pigment mixed with different ratio on the double coated paper. Mineral pigments such as clay and ground calcium carbonate(GCC) as well as hollow sphere plastic pigment were used to evaluate the physical, optical properties and printabilities of double coated paper. The physical properties such as gloss and smoothness, and the printability(ink gloss) of double coated paper were measured to evaluate the effects of the bottom layer on improving the properties of top layer. The data indicated that the usage of hollow sphere plastic pigment for the bottom layer coating improved the surface properties of double coated paper, and that ink gloss was significantly influenced by the structure of bottom layer even when hollow sphere plastic pigment was used for the bottom layer coating.