자궁내막증 환자의 복강액내 항자궁내막항체에 관한 연구

김정구,김동호,최두석,김대원,문신용,강순범,이진용,Kim, Jung-Gu,Kim, Dong-Ho,Choi, Doo-Suck,Kim, Dae-Won,Moon, Shin-Yong,Kang, Soong-Beom,Lee, Jin-Yong 대한생식의학회 1998 Clinical and Experimental Reproductive Medicine Vol.25 No.1

We have previously demonstrated that specific antigens involved in autoimmunity in endometriosis may be endometrial proteins with molecular weight (mw) of 71, 92, and 103 kilodalton (kDa). The purposes of this study were to determine the incidence of IgG antibodies against these endometrial antigens in peritoneal fluid of patients with endometriosis and to evaluate the antigenic differences between the endometria of patients with and without endometriosis. Forty peritoneal fluid (PF) from 24 patients with endometriosis and 16 patients without endometriosis (control patients) were tested against endometrial protein from patients (n=8) with endometriosis and from control patients (n=10) by western blot. Fifteen (62.5%) of 24 PF samples from patients with endometriosis had specific Immunoglobuiin (Ig) G antibodies against one of three endometrial proteins with mw of 71, 92 and 103 kDa but none of PF samples from control patients had these antibodies. The electrophoretic pattern of endometrial proteins from patients with endometriosis was similiar to that from control patients. Furthemore there was no significant difference in specific PF Immunoglobulin G binding to endometrial proteins regardless of origin of these proteins. Our data indicate that specific humoral immune response can be found in PF of patients with endometriosis and that specific antigens inducing this immune response are present in human endometrium and that there is no antigenic difference between the endometria of patients with and without endometriosis.

자궁내막증환자에서 성선자극호르몬 유리호르몬 협동제 치료후 체액성 면역인자의 변화양상

김정구,김동호,최두석,김대원,문신용,강순범,이용희,이진용,Kim, Jung-Gu,Kim, Dong-Ho,Choi, Doo-Suck,Kim, Dae-Won,Moon, Shin-Yong,Kang, Soong-Beom,Lee, Yong-Hee,Lee, Jin-Yong 대한생식의학회 2000 Clinical and Experimental Reproductive Medicine Vol.27 No.1

난관내막증의 임상 및 병리학적 특성에 관한 연구

김정구,김종혁,박태식,이경희,김석현,최영민,신창재,문신용,이진용,장윤석,Kim, Jung-Gu,Kim, Jong-Hyeok,Park, Tae-Sic,Lee, Kyung-Hee,Kim, Seok-Hyun,Choi, Young-Min,Shin, Chang-Jae,Moon, Shin-Yong,Lee, Jin-Yong,Chang, Yoon-Seok 대한생식의학회 1991 Clinical and Experimental Reproductive Medicine Vol.18 No.2

Endosalpingiosis is defined as the ectopic location of tubal epithelium. The term is purely descriptive and does not necessarily imply either the direct origin from tubal mucosa or the similarity of function. This study was performed to evaluate the clinical and pathologic characteristics of endosalpingiosis in the patients with endosalpingiosis proven by pathologic investigation. The medical histories and pathologic reports of 22 endosalpingiosis patients who received the laparotomy at Seoul National University Hospital from August, 1982 to December, 1990 were reviewed. The results were as follows: 1. Age distribution was from 26 to 46 years with the mean age of 34.0 years. 2. Twelve(54.6%) patients were nulligravida and the chief complaint of these patients was infertility(45.5%). 3. Six (27.3%) patients had the previous laparoscopic tubal sterilization by electrocautery or ring application. 4. Four (18.2%) patients had previous history of tubal pregnancy. 5. The most prevalent location of endosalpingiosis was the proximal segment of the salpinx, and the length of the proximal stump from the lesion was less than 1cm in 16(72.7%) patients.

Human Menopausal Gonadotropin으로 과배란유도된 월경주기에서의 혈청 ${\beta}$-endorphin에 과한 연구

김정구,문신용,장윤석,이진용,Kim, Jung-Gu,Mun, Shin-Yong,Chang, Yoon-Seok,Lee, Jin-Yong 대한생식의학회 1990 Clinical and Experimental Reproductive Medicine Vol.17 No.2

It has been reported that endogenous opioid peptides play a role in the control of the reproductive function. The goal of this study was to evaluate changs in the serum levels of ${\beta}$-endorphin during hyperstimulated menstrual cycle and their relationship to serum prolactin levels. Serum ${\beta}$-endorphin and prolactin levels were measured during menstrual cycles of 10 normal cylic women hyperstimulated by human menopausal gonadotropin (HMG) and of 10 women by clomiphene/HMG among in vitro fertilization candidates. The results were summarized as follows. 1. In clomiphene/HMG hyperstimulated menstrual cycle the mean serum ${\beta}$-endorphin level insignificantly on 2 day before aspiration of oocyte compared to basal level and reached maximum level on 1 day after aspiration. 2. There was a significant peak of the mean serum ${\beta}$-endorphin level on 1 day before aspiration in HMG hyperstimulated menstrual cycle. 3. On the same day from aspiration, there was no significant differences in the mean serum ${\beta}$-endorphin levels between HMG and clomiphene/HMG hyperstimulated cycles. 4. No significant correlation was noted between serum ${\beta}$-endorphin levels and prolactin levels.

여성 생삭기에 있어서의 ${\beta}$-Endorphin에 관한 면역조직학적 연구

김정구,민응기,문신용,이진용,장윤석,Kim, Jung-Gu,Min, Eung-Gi,Moon, Shin-Yong,Lee, Jin-Yong,Chang, Yoon-Seok 대한생식의학회 1988 Clinical and Experimental Reproductive Medicine Vol.15 No.1

The aim of this study was to examine the presence of ${\beta}$-endorphin in female reproductive organs. A total of 104 fresh tissue samples were obtained from normal ovary, tube, endometrium, placenta, amniotic membrane and umbilical cord, and immunostained by the method using biotin-streptoavidin amplified system. The results were as follows: 1. In reproductive age, corpus luteum only showed ${\beta}$-endorphin immunostained cells but no cells in ovaries during proliferative phase of menstrual cycle were stained. 2. Secretory endometrium revealed positive reactions in the cytoplasm of glandular epithelial cells and around the vessels, while proliferative endometrium negative reactions. 3. All the tissues of menopausal women were negative to ${\beta}$-endorphin antibody. 4. In the pregnant women, there are no ${\beta}$-endorphin containing cells in the placenta, amniotic membrane and umbilical cord regardless of gestational age.

자궁내막증에서 순환 항자궁내막 항체의 유용성

김정구,박창수,최영민,신창재,문신용,장윤석,이진용,Kim, Jung-Gu,Park, Chang-Soo,Choi, Young-Min,Shin, Chang-Jae,Moon, Shin-Yong,Chang, Yoon-Seok,Lee, Jin-Yong 대한생식의학회 1993 Clinical and Experimental Reproductive Medicine Vol.20 No.1

Antiendometrial antibodies were tested by passive hemagglutination assay(PHA) and enzyme linked immunosorbent assay(ELISA) in sera of 45 patients with endometriosis and 21 controls for the evaluation of its clinical usefulness. Both titers(reciprocal) of serum antiendometrial antibody by PHA and ELISA reactivity against endometrial antigens at 405nm were significantly higher in patients with endometriosis than in the control group. Of 45 patients with endometriosis, twenty-six(57.8%) by PHA and twenty-three(51.1%) by ELISA were determined antiendometrial antibody positive but none of the controls were positive. Antiendometrial antibody titers were followed up by PHA and ELISA in 14 patients with high antiendometrial antibody titers before and after treatment with Decapeptyl. There was a decreasing tendency of antiendometrial antibody titers after treatment but there was no statistical significance. These data suggest that the measurement of circulating antiendometrial antibody may be useful for the diagnosis but not for the follow up of endometriosis.

마그네슘 합금제 휠 제조에 관한 연구

김정구,신일성,금동화,Kim, Jung-Gu,Shin, Il-Seong,Kum, Dong-Hwa 한국재료학회 1999 한국재료학회지 Vol.9 No.7

마그네슘은 20여년간 자동차 산업에서 휠소재로 사용되어 왔다. 마그네슘 휠은 무게가 알루미늄 휠보다 25% 가벼워서 주행성이 우수하다. 이 연구의 목적은 사형주조 및 영구금형주조 공정에 의한 AZ91D 합금제 췰을 개발하는 것이다 보호개스$(SF_6+CO_2)$를 사용하는 비플럭스 용해기술을 적용하여 용탕의 산화와 불순물의 유입을 배제하였다 마그네슘 용탕은 가압식 펌프시스템을 사용하여 가열된 파이프를 통하여 모울드에 자동으로 공급된다. 열처리 및 인고트의 조성에 따른 AZ91B 합금제 휠의 기계적 특성을 조사하였다. Magnesium has been used as wheel materials in the automotive industry for more than 20 years. The magnesium wheels, which are lighter by 25% than aluminum wheels, provide easy controllability providing excellent road holding by the reduction of weight. The purpose of this work is to develop cast AZ91D alloy wheel by sand cast and permanent mold cast. The fluxless melting with the protective gas $(SF_6+CO_2)$ was Performed to eliminate oxidation of melt and impurity. The transfer of molten magnesium to the mold was done by using gas-pressurized Pump system through the heated pipe. The mechanical properites of AZ91D alloy wheel were investigated as a function of heat treatment, ingot composition.

자궁내막증 환자의 복강액내 IGF가 자궁내막 기질세포 증식에 미치는 영향

김정구,서창석,김석현,최영민,문신용,이진용,Kim, Jung-Gu,Suh, Chang-Seok,Kim, Seok-Hyun,Choi, Young-Min,Moon, Shin-Yong,Lee, Jin-Yong 대한생식의학회 1999 Clinical and Experimental Reproductive Medicine Vol.26 No.3

The purposes of this study were to evaluate the effects of insulin-like growth factor (IGF)s in peritoneal fluid (PF) from patients with and without endometriosis on the proliferation of endometrial stromal cells and to investigate the effects of type I IGF receptor antibody on the response of endometrial stromal cells to PF from patients with endometriosis. IGFs in PF from patients with endometriosis (n=14) and without endometriosis (n=10) were measured by immunoradiometric assay and PF samples were divided into low IGF-I PF group (less than 85 ng/ml) and high IGF-I PF group (more than 85 ng/ml). Endometrial stromal cells from patients without endometriosis were cultured in serum free media in the presence or absence of 1 % PF and thymidine incorporation test were used to evaluate the proliferation of endometrial stromal cells. Also cultures were incubated with type I IGF receptor monoclonal antibody (${\alpha}IR_3$) before adding PF. PF from patients with endometriosis and without endometriosis increased thymidine incorporation in endometrial stromal cells. In patients with endometriosis, high IGF-I PF group had high IGF-II levels and resulted in higher thymidine incorporation than low IGF-I PF group but no significant difference in increase in thymidine incorporation between high IGF-I and low IGF-I PF group was noted in patients without endometriosis. There was not a significant correlation between increase in thymidine incorporation and IGF-I levels in PF from patients without endometriosis but in PF from patients with endometriosis. Preincubation with ${\alpha}IR_3$ significantly inhibited the mitogenic response of endometrial stromal cells to PF. Our data indicate that IGF-I in PF may be involved in the growth of ectopic endometrium in patients with endometriosis.

조기난소부전증 환자에서의 면역학적 연구;I. 입파구아형외 변화

김정구,이진용,장윤석,Kim, Jung-Gu,Lee, Jin-Yong,Chang, Yoon-Seok 대한생식의학회 1989 Clinical and Experimental Reproductive Medicine Vol.16 No.2

The purpose of this study is to detect certain change in peripheral blood lymphocyte subpopulations in women with premature ovarian failure. The B cells, T cells and subsets were counted in 21 women with premature ovarian failure and 30 age-matched normal control women. The B cells were measured by identifying lymphocyte with surface membrane immunoglobulin and T cells and subsets by indirect immunofluorescence technique with the monoclonal antibodies OK T3, OK T4, and OK T8. The results were as follows. 1. No significant difference in the absolute number of B cells, T cells and subsets between women with premature ovarian failure and normal control women was observed. 2. The percentage of B cells, T cells and OK T8(+) cells in women with premature ovarian failure was not significantly different from that in normal control subjects respectively. 3. The percentage of OK T4(+) cells and OK T4/0K T8(+) ratio was significantly higher in women with premature ovarian failure than in control subjects.

한국 폐경여성에서 interleukin - 1 수용체 길항제의 유전자 다형성 및 골밀도의 연관성

김정구(Jung Gu Kim),김석현(Seok Hyun Kim),최영민(Young Min Choi),문신용(Shin Yong Moon),이진용(Jin Yong Lee) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.6

목적 : 한국 자연폐경 여성에서 IL-1ra 유전자 다형성양상과 골밀도 사이에 연관성이 있는가를 분석하고자 하였다. 연구 방법 : 자연폐경 여성 285명에서 PCR, 전기영동를 통하여 IL-1ra 유전자내 86bp 반복다형성 양상를 분석하고 혈청 BAP, CrossLaps 농도를 면역측정법으로, 요추 및 대퇴 근위부에서의 골밀도를 DEXA로 측정하였다. 결과 : 4종의 IL-1ra 대립인자형즉 A1 (반복횟수 4회), A2 (반복횟수 2회), A3 (반복횟수 5회), A4 (반복횟수 3회)이 관찰되었다. A2 대립인자 양성인 여성에서의 요추, 대퇴골경부, 대퇴돌기에서의 골밀도는 음성인 여성에서의 것보다 낮았다. 전자에서의 혈청 BAP 농도는 후자에서의 것보다 유의하게 높았으나 혈청 CrossLaps 농도는 차이가 없었다. IL-1ra 유전자형을 구성하는 각 대립인자에서의 반복횟수 합이 6-7인 유전자형과 그 반복횟수 합이 8-10인 유전자형에서의 골밀도와 생화학적 인자 비교시에도 유사한 경향이 관찰되었다. 정상골밀도를 가진 여성, 골감소증 여성, 골다공증여성군에서 IL-1ra A2 대립인자형의 분포양상의 차이가 없었다. 결론 : 한국 자연폐경 여성에서 골밀도는 IL-1ra A2 대립인자 양상과 연관성이 있었다. Objective : To investigate the relationship between polymorphism in interleukin-1 receptor antagonist (IL-1ra) gene and bone mineral density (BMD) in postmenopausal Korean women. Methods : The 86-base pair repeat polymorphism in the IL-1ra gene was examined by polymerase chain reaction (PCR) and electrophoresis in 285 postmenopausal Korean women. Serum bone alkaline phosphatase (BAP) and CrossLaps (CTX) were measured by immunoassay and BMD at the lumbar spine and proximal femur by dual energy X-ray absorptiometry. Results : Four alleles in IL-1ra gene were identified; A1 (4 repeats), A2 (2 repeats), A3 (5 repeats), A4 (3 repeats). BMD at the lumbar spine, femoral neck, and trochanter in women carrying A2 allele was significantly lower, compared with noncarriage of that allele. Serum BAP levels in the former group were higher than those in the latter group, but no difference in serum CTX levels between two groups was observed. There were no differences in the distribution of IL-1ra A2 allele among normal, osteopenic, and osteoporotic postmenopausal women. Conclusions : BMD are associated with a polymorphism in IL-1ra gene in postmenopausal Korean women.