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유동층 반응기에서 HZSM-5 촉매를 이용한 에탄올 전환반응에 관한 연구
김의식,이상복,김미영 ( Eui Sik Kim,Sang Bok Lee,Mi Young Kim ) 충북대학교 산업과학기술연구소 1995 산업과학기술연구 논문집 Vol.9 No.2
Abstract_Roman The characteristics of HZSM-5 catalyst was investigated by varying temperature and contact time. HZSM-5 catalyst was synthesized by hydrothermal method using a high pressure reactor. Catalysts were characterimd by X-ray diffractometer, sca
연구논문 : ECVAM 등의 국제 독성시험법 국내 확립 및 적용
이진영 ( Jin Young Lee ),강미선 ( Mi Sun Kang ),김지명 ( Ji Myoung Kim ),곽승준 ( Seung Jun Kwack ),한의식 ( Eui Sik Han ),한범석 ( Beom Seok Han ),박순희 ( Sue Nie Park ),강태석 ( Tae Seok Kang ),한순영 ( Soon Young Han ) 한국동물실험대체법학회 2010 동물실험대체법학회지 Vol.4 No.1
Repeated dose toxicity testing in rodents is used to have information on potential target organs in terms of toxicity, and the NOAEL (no-observed-adverse-effect-level). ECVAM and ICCVAM have not validated any non-animal methods for assessing chronic toxicity endpoints or repreated exposure target organ toxicity because in vivo systems often poorly resemble in vitro cell culture systems. However, recently ECVAM recommended a proposed approach for the assessment of repeated dose toxicity in vitro. We applied ECVAM`s proposal to assess target organ toxicity using liver and kidney cell lines. In order to predict liver and kidney target organ toxicities, we used three kinds of kidney toxicants(HgCl2, CH3HgCl, CdCl2) and two kinds of liver toxicants(acetaminophen, CuCl2) in human hepatoma(HepG2) cells, human renal tubular epithelial(HK-2) cells and human embryonic kidney(HEK293) cells. We performed cytotoxicity assays, mitochondria damage and calcium influx tests to predict target organ toxicity. Cell viability and cell proliferation were assessed by NRU assay and MTT assays, respectively. Mitochondria potential and cell calcium concentration([Ca2+]i) were estimated by a fluorescence microscopy using JC-1 dye and a fluorometer using Fluo-4/AM dye, respectively. The cytotoxicity of nephrotoxicants(HgCl2, CH3HgCl, CdCl2) from the result of NRU and MTT assay was higher in the kidney cells than in the liver cells. Also, the cytotoxicity of hepatotoxicants(acetaminophen, CuCl2) was higher in the liver cells than in the kidney cells. Mitochondria potential and [Ca2+]i show compatability with cytotoxicity results in the kidney cells but not in the liver cells. The results demonstrated that nephrotoxicants can predict target organ toxicity by in vitro tests like NRU, MTT assay, mitochondria potential and [Ca2+]i. However, hepatotoxicants can predict target organ toxicity by cytotoxicity assays like NRU and MTT assays but not by mitochondria potential and [Ca2+]i.
이진영 ( Jin Young Lee ),강미선 ( Mi Sun Kang ),김지명 ( Ji Myoung Kim ),곽승준 ( Seung Jun Kwack ),한의식 ( Eui Sik Han ),한범석 ( Beom Seok Han ),박순희 ( Sue Nie Park ),강태석 ( Tae Seok Kang ),한순영 ( Soon Young Han ) 한국동물실험대체법학회 2010 동물실험대체법학회지 Vol.4 No.1
Repeated dose toxicity testing in rodents is used to have information on potential target organs in terms of toxicity, and the NOAEL (no-observed-adverse-effect-level). ECVAM and ICCVAM have not validated any non-animal methods for assessing chronic toxicity endpoints or repreated exposure target organ toxicity because in vivo systems often poorly resemble in vitro cell culture systems. However, recently ECVAM recommended a proposed approach for the assessment of repeated dose toxicity in vitro. We applied ECVAM`s proposal to assess target organ toxicity using liver and kidney cell lines. In order to predict liver and kidney target organ toxicities, we used three kinds of kidney toxicants(HgCl2, CH3HgCl, CdCl2) and two kinds of liver toxicants(acetaminophen, CuCl2) in human hepatoma(HepG2) cells, human renal tubular epithelial(HK-2) cells and human embryonic kidney(HEK293) cells. We performed cytotoxicity assays, mitochondria damage and calcium influx tests to predict target organ toxicity. Cell viability and cell proliferation were assessed by NRU assay and MTT assays, respectively. Mitochondria potential and cell calcium concentration([Ca2+]i) were estimated by a fluorescence microscopy using JC-1 dye and a fluorometer using Fluo-4/AM dye, respectively. The cytotoxicity of nephrotoxicants(HgCl2, CH3HgCl, CdCl2) from the result of NRU and MTT assay was higher in the kidney cells than in the liver cells. Also, the cytotoxicity of hepatotoxicants(acetaminophen, CuCl2) was higher in the liver cells than in the kidney cells. Mitochondria potential and [Ca2+]i show compatability with cytotoxicity results in the kidney cells but not in the liver cells. The results demonstrated that nephrotoxicants can predict target organ toxicity by in vitro tests like NRU, MTT assay, mitochondria potential and [Ca2+]i. However, hepatotoxicants can predict target organ toxicity by cytotoxicity assays like NRU and MTT assays but not by mitochondria potential and [Ca2+]i.
유동층 연소로에서 진동형 공급기의 고체혼합 및 충전물에 대한 압력요동 특성
김미영,김의식 한국산업안전학회 1999 한국안전학회지 Vol.14 No.3
This study attempts to analyse the solid mixing in the feeder and the packing effect for pressure fluctuations in the fluidized bed. To study the mixing characteristics of solid in vibrating feeder for the stable operations of fluidized combustion, the system consisted of two groups of particles such that fine particles were located on the top of the coarse particles before vibratory mixing had started. The effects of packing materials on the pressure fluctuations in a fluidized bed were analysed by using a statistical method to interpret the behavior of fluidized bed. The experiments were carried out in a fluidized bed of 6.7㎝-ID, and the experimental variables were particle sizes, of 115 to 1,015㎛ in diameter and the multi-sized particles having Rosin-Rammler and Gaussian distributions. The settled bed heights of particles to diameter ratios (L/D) were ranged from 0.5 to 2.0. And fluidizing of particles was carried out by air. The packing materials used were screen packing, and the properties of the pressure fluctuations in the fluidized bed were measured by a differential pressure transducer. The properties of the pressure fluctuations calculated were the mean, the standard deviation, and the major frequency of the power spectral density functions.