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보문 : 식물 근권에서 분리한 미생물의 식물병원성 진균에 대한 길항효과 검정
김윤석 ( Yun Seok Kim ),김상우 ( Sang Woo Kim ),거비르람살 ( Kabir Lamsal ),이윤수 ( Youn Su Lee ) 한국균학회 2016 韓國菌學會誌 Vol.44 No.1
본 연구는 식물 근권에서 분리한 유용미생물 PA1, PA2, PA4, PA5, PA12 의 식물 생장 촉진능력과 식물 병원성 진균인 Colletotrichum acutatum, C. coccodes, C. gloeosporioides, C. dematium, Botrytis cinerea, Rhizoctonia solani, Sclerotinia minor 그리고 Fusarium sp.에 대한 생장억제능력을 평가하는데 그 목적이 있다. In vitro 실험에서 유용미생물의 식물 병원성 진균의 생장억제 능력을 확인하기 위해 세균배지인 TSA 배지와 곰팡이 배지인 PDA배지, 그리고 TSA와 PDA배지를 각각 50%씩 혼합한 배지(v/v, 1:1)에서 대치배양을 실시하였다. 그 결과 PDA배지에서는 PA2가 C. coccodes에 대해 65.5%로 가장 높은 억제능력을 보였으며, TSA배지에서는 PA2가 S. minor에 대해 96.5%로 가장 높은 억제력을 보였다. 또한 PDA와 TSA를 혼합한 배지에서는 PA2가 C. acutatum에 대해 58.5%로 가장 높은 억제능력을 보였다. 분리한 5균주 모두에서 식물병원성 진균에 대하여 생물적 방제 효과가 있음을 확인하였다. 또한 식물 생장 촉진능력을 유발하는 원인물질을 탐색하기 위해 siderophore, protease, chitinase, hydrogen cyanide (HCN) 생성 유무를 확인하였고, phosphate solubilizing 실험을 실시 하였다. 본 연구에서 사용된 유용미생물 5균주를 16s rDNA sequencing 결과 PA1, PA2는 Bacillus subtilis, PA4, PA5, PA12 각각 Bacillus altitudinis, Paenibacillus polymyxa, Bacillus amyloliquefaciens로 동정되었다. This study was conducted to evaluate five different strains of rhizobacterial isolates viz. PA1, PA2, PA4, PA5 and PA12 for biological control against Colletotrichum acutatum, C. coccodes, C. gloeosporioides, C. dematium, Botrytis cinerea, Rhizoctonia solani, Sclerotinia minor and Fusarium sp. In vitro inhibition assay was performed on three different growth mediums, potato dextrose agar (PDA), tryptic soy agar (TSA), and PDA-TSA (1:1 v/v) for the selection of potential antagonistic isolates. According to the result, isolate PA2 showed the highest inhibitory effect with 65.5% against C. coccodes on PDA and with 96.5% against S. minor on TSA. However, the same isolate showed the highest inhibition with 58.5% against C. acutatum on PDA-TSA. In addition, an in vivo experiment was performed to evaluate these bacterial isolates for biological control against fungal pathogens. Plants treated with bacteria were analyzed with phytopathogens and plants inoculated with phytopathogens were treated with isolates to determine the biological control effect against fungi. According to the result, all five isolates tested showed inhibitory effects against phytopathogens at various levels. Mode of action of these rhizobacterial isolates was evaluated with siderophore production, protease assay, chitinase assay and phosphate solubilizing assay. Bacterial isolates were identified by 16S rDNA sequencing, which showed that isolates PA1 and PA2 belong to Bacillus subtilis, whereas, PA4, PA5, and PA12 were identified as Bacilus altitudinis, Paenibacillus polymyxa and Bacillus amyloliquefaciens, respectively. Results of the current study suggest that rhizobacterial isolates can be used for the plant growth promoting rhizobacteria (PGPR) effect as well as for biological control of various phytopathogens.
중급 분해능 질량분석장치를 적용한 환경시료의 다이옥신 측정
최재원 ( Jae Won Choi ),김윤석 ( Youn Seok Kim ),문부식 ( Bu Shik Moon ) 한국환경분석학회 2009 환경분석과 독성보건 Vol.12 No.4
An middle resolution mass spectrometer (MRMS) with resolving power of approximately 3,000 (10% valley) was applied to the measurement of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) in environmental samples such as sediment, soil and bottom ash. Similar range of relative response factors (RRFs) obtainable in the accredited method but higher relative standard deviations (RSD) were observed by MRMS with resolving power of 1,000. Some of recovery rates using the environmental reference material (RM) significantly exceeded the criteria of quality assurance/quality control (QA/QC), possibly due to the effect of ion suppression by interfering fragments originated from the sample matrices. MRMS was applied to the quantification of RM samples for sediment, soil and bottom ash. The results were compared to those obtained using a high resolution mass spectrometer (HRMS); the same sample preparation procedures were used. Elevated concentrations of each PCDD/F congeners and toxic equivalents were obtained by MRMS: 2 times higher for river sediment and 1.7 times higher for soil and bottom ash than those from the analysis using HRMS. From the result, it was concluded that a MRMS could be used as precautional screening methods for PCDD/Fs of the samples from highly contaminated areas.
조필재(Pil-Jae Cho),김윤석(Yoon Seok Kim),이승갑(Seung-Kap Lee),윤영(Young Youn),고한서(Han Seo Ko) 대한기계학회 2004 대한기계학회 춘추학술대회 Vol.2004 No.11
A quality of a refrigeration cycle and a reliability of a compressor can be reduced if a refrigerant including excessive lubricating oil is exhausted from the compressor. Thus, the analysis of the oil behavior inside the compressor is required to prevent the problem. A tested rotary compressor with visualization windows has been manufactured in this study to investigate the oil behavior using developed visualization techniques. The oil behaviors at various operating conditions have been quantified to obtain the relationship with the outlet pressure inside the compressor. Also, the effect of the operating conditions on the quantity of the exhausted oil from the rotary compressor has been investigated using the visualization technique.
물시료 대용량 현장추출을 위한 PUF-ACF-PUF 방식의 소형 포집장치 개발과 적용: I. 다이옥신류
문부식 ( Bu Shik Moon ),김윤석 ( Youn Seok Kim ),김용연 ( Yong Yeon Kim ),최재원 ( Jae Won Choi ) 한국환경분석학회 2009 환경분석과 독성보건 Vol.12 No.4
Compact type large volume filtration system using polyurethane foam (PUF) and active carbon felt (ACF) was developed for the field sampling of persistent organic pollutants (POPs). To overcome clogging by particles in compact sampler, exception of GFF in front of PUF plug was unavoidable. Active carbon felt (ACF) layers were added between PUF slices to make up trap efficiency. Recovery tests in the lab using tap and surface water resulted in corresponding ranges of QA/QC of the accredited method for PCDD/Fs. Next, field applications for 100L volume were carried out using compact large volume sampler (LVS) with triplicates and TEQs were compared with the concentrations by conventional LVS (FS-142K model). Similar concentrations for real samples were observed between newly developed compact LVS and commercial LVS. The compact sampler would be useful as a complementary options in field water sampling.
유용 미생물을 활용한 식물 병원 곰팡이의 억제와 식물 생장촉진 효과
정진희 ( Jin Hee Jung ),김상우 ( Sang Woo Kim ),김윤석 ( Yun Seok Kim ),거비르람살 ( Kabir Lamsal ),이윤수 ( Youn Su Lee ) 한국균학회 2013 韓國菌學會誌 Vol.41 No.2
The experiment was carried out to analyze the inhibition effect of plant pathogenic fungi and growth promotion activity induced by the bacterial strains isolated from peatmoss. Among the isolated bacterial strains, B10-2, B10-4, B10-5 and B10-6 which showed more than 30% inhibition rate against Botrytis cinerea and Rhizoctonia solani in vitro, were further analyzed in the greenhouse for the growth promotion activity on lettuce (Lactuca sativa), pak-choi (Brassica compestris L. ssp. chinensis) and Chinese cabbage (Brassica campestris L. ssp. pekinensis). The results showed the treatment of B10-4 on lettuce showed the highest growth promotion activity with the leaf area (169.17 cm2), fresh weight (leaf: 40.29 g, root: 8.80 g)and dry weight (leaf: 11.24 g, root: 4.17 g), which was about two folds as compared to control. On pak-choi, the growth promotion rate was the highest with the leaf area of 112.87 cm2, leaf fresh weight of 60.70 g, root fresh weight of 3.37 g, leaf dry weight of 14.34 g, and root dry weight of 1.90 g. As a result of treatment of B10-13 on chinese cabbage, the growth promotion rate was the highest with the leaf area (293.56 cm2), fresh weight (leaf: 113.67 g, root: 2.40 g) and dry weight (leaf: 6.03 g, root: 0.53 g). The production of Indole Acetic Acid (IAA) and Indole-3-Butylic Acid (IBA) were also analyzed in these bacterial isolates. The IAA and IBA analyses were carried out in all bacterial isolates each day within the 5 days of incubation period. The highest production of IAA was observed with 112.57 μg/mg protein in B10-4 after 3 days of incubation and IBA production was the highest in B10-2 with 58.71 μg/mg protein after 2 days of incubation. Also, phosphate solubilizing activity was expressed significantly in B10-13 in comparison to that of other bacterial isolates. Bacterial identification showed that B10-2 was Bacillaceae bacterium and B10-5 was Bacillus cereus, B10-4 and B10-6 were Bacillus sp. and B-13 was Staphylococcus sp. by ITS sequence.